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991.
Catherine Deborde Mickaël Maucourt Pierre Baldet Stéphane Bernillon Benoît Biais Gilles Talon Carine Ferrand Daniel Jacob Hélène Ferry-Dumazet Antoine de Daruvar Dominique Rolin Annick Moing 《Metabolomics : Official journal of the Metabolomic Society》2009,5(2):183-198
Tomato is an essential crop in terms of economic importance and nutritional quality. In France, the third most important region
for tomato (Solanum lycopersicum L.) production is Aquitaine where the major part of production is now grown soilless under greenhouse conditions with harvest
from March to November. Tomato fruit quality at harvest is a direct function of its metabolite content at that time. The aim
of this work was to use a global approach to characterize changes in the fruit organoleptic quality at harvest under commercial
culture conditions during an entire season for two varieties and two different fertilization practices (with or without recycling
of the nutrient solution) for one variety. Absolute quantification data of 32 major compounds in fruit without seeds were
obtained through untargeted (proton nuclear magnetic resonance, 1H-NMR) quantitative profiling. These data were complemented by colorimetric analysis of ascorbate and total phenolics. They
were analyzed with chemometric approaches. Principal component analysis (PCA) or partial least square analyses (PLS) revealed
more discriminant metabolites for season than for variety and showed that nutrient solution recycling had very little effect
on fruit composition. These tendencies were confirmed with univariate analyses. 1H-NMR profiling complemented with colorimetric analyses therefore provided a diagnostic tool to follow the changes in organoleptic
and nutritional quality of tomato. In addition the quantitative information generated will help to increase our knowledge
on the mechanisms of plant response to environmental modifications.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
992.
Antoine Loquet Carole Gardiennet Christian Wasmer Anne Schütz Ronald Melki Anja Böckmann 《Journal of molecular biology》2009,394(1):108-118
The difference between the prion and the non-prion form of a protein is given solely by its three-dimensional structure, according to the prion hypothesis. It has been shown that solid-state NMR can unravel the atomic-resolution three-dimensional structure of prion fragments but, in the case of Ure2p, no highly resolved spectra are obtained from the isolated prion domain. Here, we demonstrate that the spectra of full-length fibrils of Ure2p interestingly lead to highly resolved solid-state NMR spectra. Prion fibrils formed under physiological conditions are therefore well-ordered objects on the molecular level. Comparing the full-length NMR spectra with the corresponding spectra of the prion and globular domains in isolation reveals that the globular part in particular shows almost perfect structural order. The NMR linewidths in these spectra are as narrow as the ones observed in crystals of the isolated globular domain. For the prion domain, the spectra reflect partial disorder, suggesting structural heterogeneity, both in isolation and in full-length Ure2p fibrils, although to different extents. The spectral quality is surprising in the light of existing structural models for Ure2p and in comparison to the corresponding spectra of the only other full-length prion fibrils (HET-s) investigated so far. This opens the exciting perspective of an atomic-resolution structure determination of the fibrillar form of a prion whose assembly is not accompanied by significant conformational changes and documents the structural diversity underlying prion propagation. 相似文献
993.
Michal Domanski Emil Dedic Maria Escura Prez Antoine Clry Sbastien Campagne Anne-Christine Uldry Sophie Braga Manfred Heller Julius Rabl Pavel Afanasyev Daniel Boehringer Jií Nov
ek Frdric
T Allain Oliver Mühlemann 《Nucleic acids research》2022,50(11):6300
Heterogenous nuclear ribonucleoproteins (hnRNPs) are abundant proteins implicated in various steps of RNA processing that assemble on nuclear RNA into larger complexes termed 40S hnRNP particles. Despite their initial discovery 55 years ago, our understanding of these intriguing macromolecular assemblies remains limited. Here, we report the biochemical purification of native 40S hnRNP particles and the determination of their complete protein composition by label-free quantitative mass spectrometry, identifying A-group and C-group hnRNPs as the major protein constituents. Isolated 40S hnRNP particles dissociate upon RNA digestion and can be reconstituted in vitro on defined RNAs in the presence of the individual protein components, demonstrating a scaffolding role for RNA in nucleating particle formation. Finally, we revealed their nanometer scale, condensate-like nature, promoted by intrinsically disordered regions of A-group hnRNPs. Collectively, we identify nuclear 40S hnRNP particles as novel dynamic biomolecular condensates. 相似文献
994.
Reconstructing geographical parthenogenesis: effects of niche differentiation and reproductive mode on Holocene range expansion of an alpine plant
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Bernhard Kirchheimer Karl Hülber Dietmar Moser Christoph C. F. Schinkel Marc Appelhans Simone Klatt Marco Caccianiga Agnes Dellinger Antoine Guisan Michael Kuttner Jonathan Lenoir Luigi Maiorano Diego Nieto‐Lugilde Christoph Plutzar Jens‐Christian Svenning Wolfgang Willner Elvira Hörandl Stefan Dullinger 《Ecology letters》2018,21(3):392-401
Asexual taxa often have larger ranges than their sexual progenitors, particularly in areas affected by Pleistocene glaciations. The reasons given for this ‘geographical parthenogenesis’ are contentious, with expansion of the ecological niche or colonisation advantages of uniparental reproduction assumed most important in case of plants. Here, we parameterized a spread model for the alpine buttercup Ranunculus kuepferi and reconstructed the joint Holocene range expansion of its sexual and apomictic cytotype across the European Alps under different simulation settings. We found that, rather than niche broadening or a higher migration rate, a shift of the apomict's niche towards colder conditions per se was crucial as it facilitated overcoming of topographical barriers, a factor likely relevant for many alpine apomicts. More generally, our simulations suggest potentially strong interacting effects of niche differentiation and reproductive modes on range formation of related sexual and asexual taxa arising from their differential sensitivity to minority cytotype disadvantage. 相似文献
995.
Obeid M Tesniere A Ghiringhelli F Fimia GM Apetoh L Perfettini JL Castedo M Mignot G Panaretakis T Casares N Métivier D Larochette N van Endert P Ciccosanti F Piacentini M Zitvogel L Kroemer G 《Nature medicine》2007,13(1):54-61
Anthracyclin-treated tumor cells are particularly effective in eliciting an anticancer immune response, whereas other DNA-damaging agents such as etoposide and mitomycin C do not induce immunogenic cell death. Here we show that anthracyclins induce the rapid, preapoptotic translocation of calreticulin (CRT) to the cell surface. Blockade or knockdown of CRT suppressed the phagocytosis of anthracyclin-treated tumor cells by dendritic cells and abolished their immunogenicity in mice. The anthracyclin-induced CRT translocation was mimicked by inhibition of the protein phosphatase 1/GADD34 complex. Administration of recombinant CRT or inhibitors of protein phosphatase 1/GADD34 restored the immunogenicity of cell death elicited by etoposide and mitomycin C, and enhanced their antitumor effects in vivo. These data identify CRT as a key feature determining anticancer immune responses and delineate a possible strategy for immunogenic chemotherapy. 相似文献
996.
Predicting reptile distributions at the mesoscale: relation to climate and topography 总被引:13,自引:1,他引:13
Aim To explore the respective power of climate and topography to predict the distribution of reptiles in Switzerland, hence at a mesoscale level. A more detailed knowledge of these relationships, in combination with maps of the potential distribution derived from the models, is a valuable contribution to the design of conservation strategies. Location All of Switzerland. Methods Generalized linear models are used to derive predictive habitat distribution models from eco‐geographical predictors in a geographical information system, using species data from a field survey conducted between 1980 and 1999. Results The maximum amount of deviance explained by climatic models is 65%, and 50% by topographical models. Low values were obtained with both sets of predictors for three species that are widely distributed in all parts of the country (Anguis fragilis, Coronella austriaca, and Natrix natrix), a result that suggests that including other important predictors, such as resources, should improve the models in further studies. With respect to topographical predictors, low values were also obtained for two species where we anticipated a strong response to aspect and slope, Podarcis muralis and Vipera aspis. Main conclusions Overall, both models and maps derived from climatic predictors more closely match the actual reptile distributions than those based on topography. These results suggest that the distributional limits of reptile species with a restricted range in Switzerland are largely set by climatic, predominantly temperature‐related, factors. 相似文献
997.
998.
Increasing temperature may compensate for lower amounts of dead wood in driving richness of saproxylic beetles
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![点击此处可从《Ecography》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Jörg Müller Herve Brustel Antoine Brin Heinz Bussler Christophe Bouget Elisabeth Obermaier Ina M. M. Heidinger Thibault Lachat Bernhard Förster Jakub Horak Jiří Procházka Frank Köhler Laurent Larrieu Ulrich Bense Gunnar Isacsson Livia Zapponi Martin M. Gossner 《Ecography》2015,38(5):499-509
Global warming and land‐use change are expected to be additive threats to global diversity, to which insects contribute the highest proportion. Insects are strongly influenced by temperature but also require specific habitat resources, and thus interaction between the two factors is likely. We selected saproxylic beetles as a model group because their life cycle depends on dead wood, which is highly threatened by land use. We tested the extent to which higher temperatures compensate for the negative effects of low amounts of dead wood on saproxylic beetle species richness (Temperature–Dead wood compensation hypothesis) on both a macroclimate and a topoclimate scale (north‐ and south‐facing slopes). We analyzed 1404 flight‐interception trap catches across Europe to test for interaction effects of temperature and dead‐wood amount on species richness. To experimentally test our findings from the activity trap data, we additionally reared beetles from 80 bundles of dead wood initially exposed at high and low elevations. At the topoclimate scale, we analyzed trap catches and reared beetles from dead wood exposed in 20 forest stands on south‐facing and north‐facing slopes in one region. On the macroscale, both temperature and dead‐wood amount positively affected total and threatened species richness independently, but their interaction was significantly negative, indicating compensation. On both scales and irrespective of the method, species richness decreased with temperature decline. Our observation that increasing temperature compensates for lower amounts of dead wood has two important implications. First, managers of production forests should adapt their dead‐wood enrichment strategy to site‐specific temperature conditions. Second, an increase in temperature will compensate at least partially for poor habitat conditions in production forests. Such a perspective contrasts the general assumption of reinforcing impacts of global warming and habitat loss on biodiversity, but it is corroborated by recent range expansions of threatened beetle species. 相似文献
999.
Robert Eustache Hounym Jacques Kabor Geoffrey Gimonneau Martin Bienvenu Somda Ernest Salou Antoine Abel Missihoun Zakaria Bengaly Vincent Jamonneau Alain Boulang 《PLoS neglected tropical diseases》2022,16(8)
BackgroundAnimal African Trypanosomosis (AAT) is a parasitic disease of livestock that has a major socio-economic impact in the affected areas. It is caused by several species of uniflagellate extracellular protists of the genus Trypanosoma mainly transmitted by tsetse flies: T. congolense, T. vivax and T. brucei brucei. In Burkina Faso, AAT hampers the proper economic development of the southwestern part of the country, which is yet the best watered area particularly conducive to agriculture and animal production. It was therefore important to investigate the extent of the infection in order to better control the disease. The objective of the present study was to assess the prevalence of trypanosome infections and collect data on the presence of tsetse flies.MethodsBuffy coat, Trypanosoma species-specific PCR, Indirect ELISA Trypanosoma sp and trypanolysis techniques were used on 1898 samples collected. An entomological survey was also carried out.ResultsThe parasitological prevalence of AAT was 1.1%, and all observed parasites were T. vivax. In contrast, the molecular prevalence was 23%, of which T. vivax was predominant (89%) followed by T. congolense (12.3%) and T. brucei s.l. (7.3%) with a sizable proportion as mixed infections (9.1%). T. brucei gambiense, responsible of sleeping sickness in humans, was not detected. The serological prevalence reached 49.7%. Once again T. vivax predominated (77.2%), but followed by T. brucei (14.7%) and T. congolense (8.1%). Seven samples, from six cattle and one pig, were found positive by trypanolysis. The density per trap of Glossina tachinoides and G. palpalis gambiensis was 1.2 flies.Conclusions/SignificanceOverall, our study showed a high prevalence of trypanosome infection in the area, pointing out an ongoing inadequacy of control measures. 相似文献
1000.
Prediction of Soil Organic Carbon at the European Scale by Visible and Near InfraRed Reflectance Spectroscopy 总被引:1,自引:0,他引:1
Soil organic carbon is a key soil property related to soil fertility, aggregate stability and the exchange of CO2 with the atmosphere. Existing soil maps and inventories can rarely be used to monitor the state and evolution in soil organic carbon content due to their poor spatial resolution, lack of consistency and high updating costs. Visible and Near Infrared diffuse reflectance spectroscopy is an alternative method to provide cheap and high-density soil data. However, there are still some uncertainties on its capacity to produce reliable predictions for areas characterized by large soil diversity. Using a large-scale EU soil survey of about 20,000 samples and covering 23 countries, we assessed the performance of reflectance spectroscopy for the prediction of soil organic carbon content. The best calibrations achieved a root mean square error ranging from 4 to 15 g C kg−1 for mineral soils and a root mean square error of 50 g C kg−1 for organic soil materials. Model errors are shown to be related to the levels of soil organic carbon and variations in other soil properties such as sand and clay content. Although errors are ∼5 times larger than the reproducibility error of the laboratory method, reflectance spectroscopy provides unbiased predictions of the soil organic carbon content. Such estimates could be used for assessing the mean soil organic carbon content of large geographical entities or countries. This study is a first step towards providing uniform continental-scale spectroscopic estimations of soil organic carbon, meeting an increasing demand for information on the state of the soil that can be used in biogeochemical models and the monitoring of soil degradation. 相似文献