全文获取类型
收费全文 | 13984篇 |
免费 | 1222篇 |
国内免费 | 11篇 |
专业分类
15217篇 |
出版年
2023年 | 51篇 |
2022年 | 132篇 |
2021年 | 235篇 |
2020年 | 148篇 |
2019年 | 193篇 |
2018年 | 220篇 |
2017年 | 180篇 |
2016年 | 337篇 |
2015年 | 586篇 |
2014年 | 621篇 |
2013年 | 840篇 |
2012年 | 995篇 |
2011年 | 1009篇 |
2010年 | 655篇 |
2009年 | 576篇 |
2008年 | 855篇 |
2007年 | 893篇 |
2006年 | 818篇 |
2005年 | 767篇 |
2004年 | 790篇 |
2003年 | 741篇 |
2002年 | 671篇 |
2001年 | 165篇 |
2000年 | 106篇 |
1999年 | 160篇 |
1998年 | 172篇 |
1997年 | 129篇 |
1996年 | 125篇 |
1995年 | 107篇 |
1994年 | 96篇 |
1993年 | 111篇 |
1992年 | 110篇 |
1991年 | 75篇 |
1990年 | 82篇 |
1989年 | 63篇 |
1988年 | 75篇 |
1987年 | 76篇 |
1986年 | 57篇 |
1985年 | 83篇 |
1984年 | 79篇 |
1983年 | 83篇 |
1982年 | 104篇 |
1981年 | 97篇 |
1980年 | 89篇 |
1979年 | 55篇 |
1978年 | 54篇 |
1977年 | 56篇 |
1976年 | 53篇 |
1974年 | 49篇 |
1973年 | 56篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
11.
12.
Nucleotide sequence of the Methylobacterium extorquens AM1 moxF and moxJ genes involved in methanol oxidation 总被引:10,自引:0,他引:10
The nucleotide sequence has been determined for two genes involved in methanol oxidation in the facultative methylotroph, Methylobacterium extorquens AM1. The two genes are moxF, encoding the 66-kDa subunit of the methanol dehydrogenase and moxJ, located immediately downstream from moxF, which encodes a 30-kDa protein with unknown function. This information completes the sequence of the 5.86-kb XhoI-SalI fragment containing the moxFJGI region in M. extorquens AM1, and the structure of this gene cluster is presented. Evidence is presented that moxJ is also present in Paracoccus denitrificans. The aa sequence of MoxJ has provided little information concerning its function, but it does appear to contain a signal sequence suggesting a periplasmic location. 相似文献
13.
Summary An integrated system for the collection, treatment and utilisation of piggery wastewater has been developed in Singapore which uses the cultivation of microalgae in high rate ponds to achieve reduction of BOD5 and COD5 of the effluent as well as producing single cell protein. A wide range of algal flora occurs in the ponds;Oocystis, Micratinium, Scenedesmus, Ankistrodesmus, Chlorella andOscillatoria spp were identified. Total algal counts, recorded from 1979 to 1981, ranged up to 107 per ml of pond water. There were considerable variations in the algal population and in the predominating species. No discernible pattern was evident. Consequently pond operations were frequently disturbed by these fluctuations in population which in turn was attributed to the heterogeneous composition of the piggery waste, to variable weather conditions and to predation by larger organisms particularlyMoina. After passing through the ponds, the total suspended solids were removed by a novel dissolved air flotation method which gave a clear effluent showing an 87% reduction in BOD5 value.
Resumen En Singapur se ha desrrollado un sistema integrado para le recogida, tratamiento y utilización de aguas residuales de pocilgas. Este sistema usa el cultivo de microalgas en estanques, de caudal rápido afin de reducir las DBO5 y DCO5 del efluencte produciendo asimismo proteínas celulares. La flora de algas producida en estos estanques es amplia y variada, habiendose identificado:Oocystis, Micratinium, Scenesdemus, Ankistrodesmus, Chlorella y Oscillatoria. Los recuentos totales de algas tomados desde 1979 a 1981 llegaron a alcanzar 10;7 por ml de agua del estanque. Se observaron variaciones considerables tanto en la población total de algas como en las especies predominantes sin que se pudiese, determinar un patrón de variación característico. Estas fluctuaciones en la población, causantes de frecuentes alteraciones en el funcionamiento del estanque, se atribuyeron a la composición heterogenea de los resuduos, a las variaciones climáticas y la predación por otros organismos particularmenteMoina Después de su paso por los estanque los solidos suspendidos totales se eliminaron mediante un nuevo método de flotación con aire disuelto, obteniendose un efluente limpio con una reducción de la DBO5 del 87%.
Résumé Un système intégré pour la collecte, le traitement et l'utilisation d'eaux résiduaires de porcherie a été développé à Singapoure. Il utilise la culture de micro-algues tant pour la réduction accélérée en lagune de la DBO5 et de la DCO de l'effluent que pour la production de protéines uni-cellulaires. On trouve une large gamme de flore algale dans les lagunes; des espèces d'-Oocystis, deMicratinium, deScenedesmus, Ankistrodesmus, deChlorella etd'Oscillatoria out été identifiées. Les comptages totaux d'algues, enregistrées de 1979 à 1981, ont donné jusqu' à 107 cellules par ml d'eau de la lagune. On a observé des variations considérables de population algale tant quantitatives que qualitatives. On ne discernait pas de spectres évidents. En conséquence, les opérations lagunaires ont été fréquemment perturbées par ces fermentations en population, qui, à leur tour, ont été attribuées à la composition hétérogène de l'effluent de porcherie, aux conditions atmosphériques et climatiques variables et à la prédation par des organismes plus conséquents, plus particulièrement desMoina. Après passage par les lagunes, les solides totaux en suspension ont été enlevés par une méthode nouvelle de flottation à l'air dissous, qui a donné un effluent limpide, présentant une réduction de DBO5 de 87%.相似文献
14.
15.
16.
17.
Identification of lactaldehyde dehydrogenase and glycolaldehyde dehydrogenase as functions of the same protein in Escherichia coli 总被引:6,自引:0,他引:6
E Caballero L Baldomá J Ros A Boronat J Aguilar 《The Journal of biological chemistry》1983,258(12):7788-7792
Lactaldehyde dehydrogenase is an enzyme involved in the aerobic metabolism of fucose in wild type Escherichia coli, and glycolaldehyde dehydrogenase is an enzyme involved in the metabolism of ethylene glycol in mutant cells able to utilize this glycol. Both enzyme sources display oxidative activity on either substrate with a constant ratio between these activities. We have found that both enzymatic activities present the same electrophoretic mobility when crude extracts were electrophoresed in polyacrylamide gels and the gels stained for enzyme activities. Furthermore, both enzymatic activities co-chromatograph in a DEAE-Sephadex column. If lactaldehyde dehydrogenase of wild type cells is purified near homogeneity and the purification procedure is screened for both aldehydes as substrates, only one enzyme is apparent, giving again a constant ratio between lactaldehyde and glycolaldehyde dehydrogenase activities. Genetic evidence of the fact that both activities are functions of the same protein is provided by the observation that mutation to thermosensitivity for the production of lactaldehyde dehydrogenase affected in the same way the production of glycolaldehyde dehydrogenase. Glycolaldehyde dehydrogenase from mutant cells is purified in a procedure coincident with the lactaldehyde dehydrogenase purification, yielding a single enzyme electrophoretically indistinguishable from the purified lactaldehyde dehydrogenase. Peptide mapping of the purified preparation after digestion with chymotrypsin or Staphylococcus aureus protease V8 gives an indistinguishable band pattern between both enzymes. 相似文献
18.
Neil S. Painter Anthony Z. Almeida Kenneth W. Colebourne 《BMJ (Clinical research ed.)》1972,2(5806):137
Seventy patients with diverticular disease of the colon were treated with a high-residue, low-sugar diet including unprocessed bran. Follow-up for an average of 22 months showed marked relief of symptoms in 62 patients. Bowel habit was restored towards normal and abdominal discomfort relieved. Only seven patients were unable to give up the use of laxatives. Eight patients did not tolerate the bran diet, and one of these needed surgical treatment. None of the 62 patients who took the diet needed surgery. 相似文献
19.
20.
Anthony J. Hedley Anne M. Scott R. Deans Weir James Crooks 《BMJ (Clinical research ed.)》1970,1(5695):556-558
An automated follow-up register for the detection of iatrogenic thyroid disease has been established as a joint venture between the general practitioners in the north-east of Scotland and the thyroid clinic of Aberdeen General Hospitals.The data-processing operations in the system are handled by an International Computers Limited 4/50 computer. Patients are followed up at predetermined intervals and the system has been designed to process, screen, and store clinical and biochemical follow-up data and report results to the patients, general practitioners, and the hospital records department. 相似文献