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151.
Field evaluation of a sound system to reduce estuarine fish intake rates at a power plant cooling water inlet 总被引:1,自引:0,他引:1
J. Maes † A. W. H. Turnpenny ‡ D. R. Lambert ‡ J. R. Nedwell ‡ A. Parmentier § F. Ollevier 《Journal of fish biology》2004,64(4):938-946
An acoustic deterrent system producing 20–600 Hz sound was used to repel estuarine fishes away from a power station cooling water inlet. During sound emission, total fish impingement decreased by 60%. The avoidance response varied among species from no effect to highly efficient deflection. Lampetra fluviatilis and Pleuronectiformes were less affected by the sound system while the deflection of clupeoid species was particularly effective. Average intake rates of Clupea harengus and Sprattus sprattus decreased by 94·7 and 87·9%, respectively. The results were explained as a function of species‐specific differences in hearing ability and swimming performance. In general, species without swimbladders showed no or a moderate response while intake rates of species with accessory structures increasing the hearing abilities, such as a swimbladder or a functional connection between the swimbladder and the inner ear, were significantly reduced during test periods. 相似文献
152.
Hakkoum D Imhof A Vallet PG Boze H Moulin G Charnay Y Stoppini L Aronow B Bouras C Giannakopoulos P 《Journal of neurochemistry》2008,106(4):1791-1803
Clusterin or apolipoprotein J is a heterodimeric glycoprotein which is known to be increased during tissue involution in response to hormonal changes or injury and under circumstances leading to apoptosis. Previous studies in wild-type (WT) and clusterin-null (Clu−/−) mice indicated a protective role of clusterin over-expression in astrocytes lasting up to 90 days post-ischemia. However, in in vitro and in vivo models of neonatal hypoxia-ischemia, clusterin exacerbates necrotic cell death. We developed recombinant forms of clusterin and examined their effect on propidium iodide uptake, neuronal and synaptic markers as well as electrophysiological recordings in hippocampal slice cultures from Clu−/− and WT mice subjected to oxygen-glucose deprivation (OGD). WT mice displayed a marked up-regulation of clusterin associated with electrophysiological deficits and dramatic increase of propidium iodide uptake 5 days post-OGD. Immunocytochemical and western blot analyses revealed a substantial decrease of neuronal nuclei and synaptophysin immunoreactivity that predominated in WT mice. These findings contrasted with the relative post-OGD resistance of Clu−/− mice. The addition of biologically active recombinant forms of human clusterin for 24 h post-OGD led to the abolishment of the ischemic tolerance in Clu−/− slices. This deleterious effect of clusterin was reverted by the concomitant administration of the NMDA receptor antagonist, d -2-amino-5-phosphonopentanoate. The present data indicate that in an in vitro model of ischemia characterized by the predominance of NMDA-mediated cell death, clusterin exerts a negative effect on the structural integrity and functionality of hippocampal neurons. 相似文献
153.
154.
Gut contents of sand goby Pomatoschistus minutus showed higher C and N isotope values than the food before consumption. This enrichment was more pronounced in the hindgut than in the foregut, probably because of preferential assimilation of 12 C and 14 N along the gastro-intestinal tract. The results indicated that the shift towards higher values in the alimentary canal occurs in the first 2 h after feeding. 相似文献
155.
Ingrid Dijkgraaf Pieter Van de Vijver Anouk Dirksen Tilman M. Hackeng 《Bioorganic & medicinal chemistry》2013,21(12):3555-3564
Angiogenesis is a multi-step process regulated by pro- and anti-angiogenic factors. Inhibition of angiogenesis is a potential anti cancer treatment strategy that is now investigated clinically. In addition, advances in the understanding of the angiogenic process have led to the development of new angiogenesis therapies for ischemic heart disease.Currently, researchers search for objective measures that indicate pharmacological responses to pro- and anti-angiogenic drugs and therefore, there is a great interest in techniques to visualize angiogenesis noninvasively. As CD13 is selectively expressed in angiogenic blood vessels, it can serve as a target for molecular imaging tracers to noninvasively visualize angiogenic processes in animal models and patients. Here, an overview on the currently used CD13 targeted molecular imaging probes for noninvasive visualization of angiogenesis is given. 相似文献
156.
p53 is a major suppressor of human malignancy. The protein levels and activity are tightly regulated in cells. Early experiments
identified nuclear localization signal 1 (NLS1) as a regulator of p53 localization. We have generated mice bearing a mutation
in p53
NLS1
, designated p53
NLS1
. Our experiments confirm a role for NLS1 in regulating p53 function. Murine embryonic fibroblasts generated from homozygous
p53
NLS1
animals are partially defective in cell cycle arrest and do not respond to inhibitory signals from oncogenic Ras. In addition, p53-dependent apoptosis is abrogated in thymocytes. Contrary to predicted results, fibroblasts from homozygous
p53
NLS1
animals have a greater rate of proliferation than p53-null cells. In addition, p53
NLS1
cells are more resistant to UV-induced death. Surprisingly, the homozygous p53
NLS1
animals exhibit embryonic and peri-natal lethality, with a significant portion of the animals developing exencephaly. Thus,
p53
NLS1/NLS1
embryos exhibit a reduced viability relative to p53-null mice. These studies indicate that the NLS1 is a major regulator of p53 activity in vivo. 相似文献
157.
Eszter Majer Zaira Salvador Mark P. Zwart Anouk Willemsen Santiago F. Elena José-Antonio Daròs 《Journal of virology》2014,88(8):4586-4590
Potyviruses express most of their proteins from a long open reading frame that is translated into a large polyprotein processed by three viral proteases. To understand the constraints on potyvirus genome organization, we relocated the viral RNA-dependent RNA polymerase (NIb) cistron to all possible intercistronic positions of the Tobacco etch virus (TEV) polyprotein. Only viruses with NIb at the amino terminus of the polyprotein or in between P1 and HC-Pro were viable in tobacco plants. 相似文献
158.
Huang H Lavoie-Lamoureux A Moran K Lavoie JP 《American journal of physiology. Lung cellular and molecular physiology》2007,292(5):L1147-L1154
Little is known concerning the possible contribution of T helper 2 (Th2)-type cytokines to the recruitment of neutrophils into the lung tissue. In the present study, endothelial cells from equine pulmonary arteries were cultured in the presence of recombinant equine (re) IL-4 and reIL-5, and the cytokine mRNA expression of molecules implicated in the chemotaxis and migration of neutrophils was studied using real-time RT-PCR. The functional response of reIL-4-induced endothelial cell stimulation on neutrophil migration was also studied using a chemotaxis chamber. ReIL-4 either increased the expression of CXCL-8, E-selectin, vascular endothelial growth factor (VEGF), and inducible nitric oxide synthase (iNOS), or potentiated the coeffects of lipopolysaccharide (LPS) and tumor necrosis factor-alpha (TNF-alpha) on CXCL-8. Supernatants collected from cultured endothelial cells stimulated with reIL-4 significantly promoted neutrophil migration in a dose-dependent manner. Dexamethasone (DXM) decreased the expression of CXCL-8, VEGF, and iNOS induced by reIL-4, while 1400W dihydrochloride (1400W), a selective inhibitor of iNOS, decreased the expression of E-selectin, VEGF, and iNOS. DXM and 1400W attenuated the mRNA expression of E-selectin and iNOS induced by the costimulation of reIL-4, reTNF-alpha, and LPS. Neither equine nor human recombinant IL-5 influenced the mRNA expression of CXCL-8, E-selectin, or VEGF. These findings suggest that Th2-type cytokines may contribute to pulmonary neutrophilia during allergic inflammation by the increased expression of neutrophil chemokines and adhesion molecules by endothelial cells. DXM and the iNOS inhibitors may decrease pulmonary neutrophilia due, in part, to a direct inhibition of some of these factors. 相似文献
159.
Metacaspase activity of Arabidopsis thaliana is regulated by S-nitrosylation of a critical cysteine residue 总被引:7,自引:0,他引:7
Belenghi B Romero-Puertas MC Vercammen D Brackenier A Inzé D Delledonne M Van Breusegem F 《The Journal of biological chemistry》2007,282(2):1352-1358
Nitric oxide (NO) regulates a number of signaling functions in both animals and plants under several physiological and pathophysiological conditions. S-Nitrosylation linking a nitrosothiol on cysteine residues mediates NO signaling functions of a broad spectrum of mammalian proteins, including caspases, the main effectors of apoptosis. Metacaspases are suggested to be the ancestors of metazoan caspases, and plant metacaspases have previously been shown to be genuine cysteine proteases that autoprocess in a manner similar to that of caspases. We show that S-nitrosylation plays a central role in the regulation of the proteolytic activity of Arabidopsis thaliana metacaspase 9 (AtMC9) and hypothesize that this S-nitrosylation affects the cellular processes in which metacaspases are involved. We found that AtMC9 zymogens are S-nitrosylated at their active site cysteines in vivo and that this posttranslational modification suppresses both AtMC9 autoprocessing and proteolytic activity. However, the mature processed form is not prone to NO inhibition due to the presence of a second S-nitrosylation-insensitive cysteine that can replace the S-nitrosylated cysteine residue within the catalytic center of the processed AtMC9. This cysteine is absent in caspases and paracaspases but is conserved in all reported metacaspases. 相似文献