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991.
Pereira JM Severino RP Vieira PC Fernandes JB da Silva MF Zottis A Andricopulo AD Oliva G Corrêa AG 《Bioorganic & medicinal chemistry》2008,16(19):8889-8895
Chagas' disease, a parasitic infection caused by the flagellate protozoan Trypanosoma cruzi, is a major public health problem affecting millions of individuals in Latin America. On the basis of the essential role in the life cycle of T. cruzi, the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH) has been considered an attractive target for the development of novel antitrypanosomatid agents. In the present work, we describe the inhibitory effects of a small library of natural and synthetic anacardic acid derivatives against the target enzyme. The most potent inhibitors, 6-n-pentadecyl- and 6-n-dodecylsalicilic acids, have IC(50) values of 28 and 55 microM, respectively. The inhibition was not reversed or prevented by the addition of Triton X-100, indicating that aggregate-based inhibition did not occur. In addition, detailed mechanistic characterization of the effects of these compounds on the T. cruzi GAPDH-catalyzed reaction showed clear noncompetitive inhibition with respect to both substrate and cofactor. 相似文献
992.
A. Cruz B. Angelova P. Fernandes J. M. S. Cabral H. M. Pinheiro 《Biocatalysis and Biotransformation》2004,22(3):189-194
The selective side-chain cleavage of β-sitosterol by free cells of Mycobacterium sp. NRRL B-3805 is a well-established multi-enzymatic process for the production of the pharmaceutical steroid precursors androstenedione (AD) and androstadienedione (ADD). In this study, bis(2-ethylhexyl) phthalate (BEHP) was used as a reaction medium for carrying out the process with freely suspended cells. The work aimed to show that microbial sitosterol side-chain cleavage is possible in this essentially mono-phasic organic medium, provided that some important parameters are adequately controlled. The effects of the biocatalyst/substrate mass ratio, system aeration rate and minimum buffer addition to the organic medium on the product yield and the reaction rate were thus evaluated. 相似文献
993.
Sucrose Metabolism in Lupinus albus L. Under Salt Stress 总被引:3,自引:0,他引:3
Salt stress (50 and 150 mM NaCl) effects on sucrose metabolism was determined in Lupinus albus L. Sucrose synthase (SS) activity increased under salt stress and sucrose phosphate synthase activity decreased. Acid invertase
activity was higher at 50 mM NaCl and decreased to control levels at 150 mM NaCl. Alkaline invertase activity increased with
the salt stress. Glucose content decreased with salt stress, sucrose content was almost three times higher in plants treated
with 150 mM NaCl and fructose content did not change significantly. The most significant response of lupin plants to NaCl
excess is the increase of sucrose content in leaves, which is partially due to SS activity increase under salinity.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
994.
Claudino MJ Soares D Van Keulen F Marques MP Cabral JM Fernandes P 《Bioresource technology》2008,99(7):2304-2311
Silicone rubbers are hydrophobic, a feature that may prove advantageous if this material is to be used as immobilization matrix in bioconversion systems where hydrophobic species are present, such as sterols and mycobacterial cells. Mycobacterium sp. cells with sitosterol side chain cleavage activity were accordingly effectively adsorbed onto silicone and the potential application of the concept was assessed by matching the behavior of the resulting immobilized biocatalyst with free cells and Celite immobilized cells. Mass transfer, kinetics, thermal and storage stability characterization of a biotransformation system based in the use of the silicone immobilized biocatalyst was performed. The feasibility of biocatalyst reutilization was tentatively explored. 相似文献
995.
Fernandes L Fischer FL Ribeiro CW Silveira GP Sá MM Nome F Terenzi H 《Bioorganic & medicinal chemistry letters》2008,18(16):4499-4502
Hydrolysis of DNA is of increasing importance in biotechnology and medicine. In this Letter, we present the DNA-cleavage potential of metal-free hydroxylamines and oximes as new members of nucleic acid cleavage agents. 相似文献
996.
We have examined the effect of the uncharged species of lidocaine (LDC) and etidocaine (EDC) on the acyl chain moiety of egg phosphatidylcholine liposomes. Changes in membrane organization caused by both anesthetics were detected through the use of EPR spin labels (5, 7 and 12 doxyl stearic acid methyl ester) or fluorescence probes (4, 6, 10, 16 pyrene-fatty acids). The disturbance caused by the LA was greater when the probes were inserted in more external positions of the acyl chain and decreased towards the hydrophobic core of the membrane. The results indicate a preferential insertion of LDC at the polar interface of the bilayer and in the first half of the acyl chain, for EDC. Additionally, (2)H NMR spectra of multilamellar liposomes composed by acyl chain-perdeutero DMPC and EPC (1:4 mol%) allowed the determination of the segmental order (S(mol)) and dynamics (T(1)) of the acyl chain region. In accordance to the fluorescence and EPR results, changes in molecular orientation and dynamics are more prominent if the LA preferential location is more superficial, as for LDC while EDC seems to organize the acyl chain region between carbons 2-8, which is indicative of its positioning. We propose that the preferential location of LDC and EDC inside the bilayers creates a "transient site", which is related to the anesthetic potency since it could modulate the access of these molecules to their binding site(s) in the voltage-gated sodium channel. 相似文献
997.
Avilez IM Hori TS de Almeida LC Hackbarth A da Cunha Bastos Neto J da Cunha Bastos VL Moraes G 《Comparative biochemistry and physiology. Toxicology & pharmacology : CBP》2008,148(2):136-142
Parameters of the antioxidant defense systems of Brycon amazonicus (matrinx?--a neotropical fish) exposed to phenol for 96 h plus the recovery over 1 and 2 weeks were studied in erythrocytes and liver. Hematocrit increase was observed during phenol exposure and recovery for 1 week. Total superoxide dismutases (SOD), glutathione peroxidase (GPx) and reduced glutathione (GSH) did not change during phenol exposure. Erythrocyte glucose-6-phosphate dehydrogenase (G6PDH) increased during that period while catalase (CAT) activity decreased during phenol exposure and recovery for 2 weeks. In the liver, SOD and CAT did not change, whereas GPx increased in the first week of recovery and decreased after 2 weeks. A late response was observed for G6PDH activity which increased only at the second week. Ascorbate concentration in the brain decreased during phenol exposure and increased over recovery. From our results it appears that the oxidative stress was limited in matrinx? exposed to phenol, but seemed to occur during the recovery period. 相似文献
998.
Carlos A. Fernandes Catarina Ginja Iris Pereira Rogério Tenreiro Michael W. Bruford Margarida Santos-Reis 《Conservation Genetics》2008,9(3):681-690
Genetic species identification of non-invasively collected samples has become an important tool in ecological research, management
and conservation and wildlife forensics. This is especially true for carnivores, due to their elusive nature, and is crucial
when several ecologically and phylogenetically close species, with similar faeces, hairs, bones and/or pelts, occur in sympatry.
This is the case of the Iberian Peninsula, a region with a carnivore community of 16 species—about two-thirds of the European
carnivore fauna. Here we present a simple, efficient and reliable PCR-based protocol, using a novel set of species-specific
primers, for the unambiguous identification to species of non-invasively collected samples or forensic materials from Iberian
carnivores. For each species, from the consensus of all cytochrome b haplotypes, found here and previously reported, we designed species-specific primer pairs for short fragments, the most likely
to persist in low-quantity and degraded DNA samples. The predicted specificity of each primer pair was assessed through PCR
of positive DNA extracts from the carnivore species, from an exhaustive array of potential prey and from humans. The robustness
of PCR amplification for non-invasively sampled DNA was tested with scat samples. The primers did not produce false positives
and correctly identified all carnivore samples to the species level. In comparison with sequencing and PCR-RFLP assays, our
method is, respectively, cost- and time-effective, and is especially suited for monitoring surveys targeting multiple populations/species.
It also introduces an approach that works for a whole community of carnivores living sympatrically over a large geographic
area. 相似文献
999.
Michelle Andrade Furtado Lenita Caetano Fernandes de Almeida Ricardo Andrade Furtado Wilson Roberto Cunha Denise Crispim Tavares 《Mutation Research - Genetic Toxicology and Environmental Mutagenesis》2008,657(2):150-154
Rosmarinic acid (RA) is a natural phenolic compound which presents different biological activities such as antitumor, antibacterial, anti-inflammatory, hepatoprotective and cardioprotective properties. In view of its important biological activities, the study of the effects of RA on genetic material becomes relevant. Thus, the objective of the present study was to evaluate the mutagenic and/or antimutagenic potential of RA on peripheral blood cells of Swiss mice using the micronucleus assay. Three doses of RA (50, 100 and 200 mg/kg body weight, b.w.) were used for the evaluation of its mutagenic potential. In the antimutagenicity assays, the different concentrations of RA were combined with the chemotherapeutic agent doxorubicin (DXR, 15 mg/kg b.w.). Peripheral blood samples were collected 24, 48 and 72 h after treatment for the evaluation of micronucleated polychromatic erythrocytes (MNPCEs). The results of the mutagenicity assays showed no increase in the frequency of micronuclei in animals treated with different concentrations of RA when compared to the negative controls. Treatment with different concentrations of RA combined with DXR revealed a significant reduction in the frequency of micronuclei compared to animals treated with DXR only. Although the mechanisms underlying the protective effect of RA are not completely understood, the putative antioxidant activity of RA might explain its effect on DXR mutagenicity. 相似文献
1000.
Buckup L Dutra BK Ribarcki FP Fernandes FA Noro CK Oliveira GT Vinagre AS 《Comparative biochemistry and physiology. Part A, Molecular & integrative physiology》2008,149(1):59-67
The crayfish Parastacus defossus occurs in Uruguay and the state of Rio Grande do Sul in Brazil. It lives in swamps and lakes, where it digs sloped subterranean tunnels that are used as burrows. Because there is little information about the biology, physiology and ecology of this species, the aim of this study was to identify the seasonal variations of its carbohydrate and lipid metabolism. Crayfish were collected monthly (from November 2002 to February 2004) in the Lami region, Porto Alegre municipality (30 degrees 11'41'S-51 degrees 06'00'W). Haemolymph samples, used for determination of glucose, total proteins, triglycerides, total cholesterol and total lipids, were collected in the field using potassium oxalate as an anti-clotting agent. The animals and haemolymph samples were immediately frozen in the field. In the laboratory, the hepatopancreas, gills and abdominal muscles were removed for determination of glycogen, triglycerides, total cholesterol and total lipids. The findings suggest that in P. defossus, lipids are an important reserve of energy used during reproduction in both males and females; whereas glycogen may be used during periods of intense activity or environmental stress. 相似文献