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81.
Lactoferrin and host defence: an overview of its immuno-modulating and anti-inflammatory properties 总被引:1,自引:0,他引:1
Lactoferrin is a member of the transferrin family of iron-binding glycoproteins that is abundantly expressed and secreted from glandular epithelial cells. In secretions, such as milk and fluids of the intestinal tract, lactoferrin is an important component of the first line of host defence. During the inflammatory process, lactoferrin, a prominent component of the secondary granules of neutrophils (PMNs), is released in infected tissues and in blood and then it is rapidly cleared by the liver. In addition to the antimicrobial properties of lactoferrin, a set of studies has focused on its ability to modulate the inflammatory process and the overall immune response. Though many in vitro and in vivo studies report clear regulation of the immune response and protective effect against infection and septic shock by lactoferrin, elucidation of all the cellular and molecular mechanisms of action is far from being achieved. At the cellular level, lactoferrin modulates the migration, maturation and function of immune cells. At the molecular level and in addition to iron binding, interactions of lactoferrin with a plethora of compounds, either soluble or membrane molecules, account for its modulatory properties. This paper reviews our current understanding of the cellular and molecular mechanisms that explain the regulatory properties of lactoferrin in host defence. 相似文献
82.
Bernard Pineau Jacqueline Girard-Bascou Stephan Eberhard Yves Choquet Antoine Trémolières Catherine Gérard-Hirne Annick Bennardo-Connan Paulette Decottignies Sylvie Gillet Francis-André Wollman 《European journal of biochemistry》2004,271(2):329-338
Two mutants of Chlamydomonas reinhardtii, mf1 and mf2, characterized by a marked reduction in their phosphatidylglycerol content together with a complete loss in its Delta3-trans hexadecenoic acid-containing form, also lost photosystem II (PSII) activity. Genetic analysis of crosses between mf2 and wild-type strains shows a strict cosegregation of the PSII and lipid deficiencies, while phenotypic analysis of phototrophic revertant strains suggests that one single nuclear mutation is responsible for the pleiotropic phenotype of the mutants. The nearly complete absence of PSII core is due to a severely decreased synthesis of two subunits, D1 and apoCP47, which is not due to a decrease in translation initiation. Trace amounts of PSII cores that were detected in the mutants did not associate with the light-harvesting chlorophyll a/b-binding protein antenna (LHCII). We discuss the possible role of phosphatidylglycerol in the coupled process of cotranslational insertion and assembly of PSII core subunits. 相似文献
83.
Borges JP Barre A Culerrier R Granier C Didier A Rougé P 《Biochemical and biophysical research communications》2008,365(4):685-690
Four IgE-binding epitopes have been characterized that cover a large area (40%) of the molecular surface of lipid transfer protein allergens of Rosaceae (apple, peach, apricot, and plum). They mainly correspond to electropositively charged regions protruding on the molecular surface of the modeled apple (Mal d 3), apricot (Pru ar 3), and plum (Pru d 3) allergens. Two of these epitopes consist of consensus epitopes structurally conserved among the lipid transfer protein allergens from the Rosaceae. Their occurrence in different lipid transfer protein allergens presumably accounts for the IgE-binding cross-reactivity often observed among different Rosaceae fruits. In this respect, LTP consist of phylogenetically- and structurally-related pan allergens. However, the IgE-binding cross-reactivity due to fruit lipid transfer protein has varying degrees of clinical relevance and this cross-reactivity is not necessarily accompanied by a cross-allergenicity to the corresponding fruits. 相似文献
84.
María Suárez-Cervera Elsa Arcalís Annick Le Thomas Juan A. Seoane-Camba 《Sexual plant reproduction》2002,14(5):291-298
The apertural inner layer (intine) of Euphorbia L. pollen grains has a characteristic but original structure that has paired thickenings, one on either side of the colpus. To determine the nature and role of this intine layer, pollen grains of Euphorbia peplus L. were germinated in vivo and in vitro. The germination process involves wall changes that facilitate formation of the pollen tube and its subsequent growth. In the thickenings of the intine of E. peplus, the unesterified pectin epitopes are more densely localised in the inner part of the middle intine. No such epitopes are located in the intine portion adjacent to the plasma membrane (cellulosic endintine). Unesterified pectin epitopes are also localised in the outer part of the intine but are restricted to the centre of the aperture, around and in the pore. The de-esterification of pectins is very advanced at the time of dehiscence and pollen germination. The stratification of the aperture intine may take the following pathway at the time of germination: the thin outer zone of the intine in the pore region becomes disorganised and undergoes dissolution with liberation of unesterified and esterified pectins; the middle intine thickenings undergo an important elastic modification, but without liberation of unesterified pectins; the cellulosic inner intine is the progenitor of the pollen tube wall. This special intine of E. peplus is an adaptation to the hydration process preceding germination, increasing intine and pollen grain wall elasticity. 相似文献
85.
Véronique Quillien Annick Moisan Thierry Lesimple Claudine Leberre Louis Toujas 《Cancer immunology, immunotherapy : CII》2001,50(9):477-482
PURPOSE: We have previously reported a clinical trial on the intravenous injection of autologous activated macrophages (AAM) in 15 patients with renal carcinoma. The present paper concerns scintigraphic investigations performed in 11 of these patients after injection of 111indium oxinate-radiolabeled AAM. METHODS: AAM were prepared from mononuclear cells (MNC) collected by apheresis from patients treated simultaneously with granulocyte-macrophage colony-stimulating factor (GM-CSF). MNC were cultured for 6 days in the presence of GM-CSF and exposed for 18 h to gamma-interferon, the AAM were then separated by elutriation and injected. RESULTS: After intravenous infusion, radiolabeled AAM were transiently retained in the lungs, where they predominated in the first hour. Later on, radioactivity accumulated in liver and spleen and then decreased from the first and second day, respectively. In one patient, two foci of radioactivity were detected in the lungs 1 h after injection, and persisted thereafter. Their association with tumor lesions was uncertain. This observation possibly resulted from the presence of granulocytes in the radiolabeled AAM populations of this patient. It seems that MNC collected from GM-CSF-treated patients and cultured in the presence of GM-CSF enables the differentiation of granulocytes. CONCLUSIONS: A series of 11 investigations confirms the previously reported distribution pattern of intravenously injected AAM. It is possible that in patients treated with hematopoietic cell-mobilizing agents, granulocytes develop in cultures designed to produce monocyte-derived antigen-presenting cells. 相似文献
86.
Julie M. Koeman Ryan C. Russell Min-Han Tan David Petillo Michael Westphal Katherine Koelzer Julie L. Metcalf Zhongfa Zhang Daisuke Matsuda Karl J. Dykema Heather L. Houseman Eric J. Kort Laura L. Furge Richard J. Kahnoski Stphane Richard Annick Vieillefond Pamela J. Swiatek Bin Tean Teh Michael Ohh Kyle A. Furge 《PLoS genetics》2008,4(9)
87.
Dieter Schouppe Pierre Rougé Yi Lasanajak Annick Barre David F. Smith Paul Proost Els J. M. Van Damme 《Glycoconjugate journal》2010,27(6):613-623
At present the three-dimensional structure of the tobacco lectin, further referred to as Nictaba, and its carbohydrate-binding
site are unresolved. In this paper, we propose a three-dimensional model for the Nictaba domain based on the homology between
Nictaba and the carbohydrate-binding module 22 of Clostridium thermocellum Xyn10B. The suggested model nicely fits with results from circular dichroism experiments, indicating that Nictaba consists
mainly of β-sheet. In addition, the previously identified nuclear localization signal is located at the top of the protein
as a part of a protruding loop. Judging from this model and sequence alignments with closely related proteins, conserved glutamic
acid and tryptophan residues in the Nictaba sequence were selected for mutational analysis. The mutant DNA sequences as well
as the original Nictaba sequence have been expressed in Pichia pastoris and the recombinant proteins were purified from the culture medium. Subsequently, the recombinant proteins were characterized
and their carbohydrate binding properties analyzed with glycan array technology. It was shown that mutation of glutamic acid
residues in the C-terminal half of the protein did not alter the carbohydrate-binding activity of the lectin. In contrast,
mutation of tryptophan residues in the N-terminal half of the Nictaba domain resulted in a complete loss of carbohydrate binding
activity. These results suggest that tryptophan residues play an important role in the carbohydrate binding site of Nictaba. 相似文献
88.
Annick Ohayon 《PSN》2003,1(4):50-61
A sexological movement look form in France in the 1930’s on the initiative of the alienist Dr. Edouard Toulouse. The movement combined the approach of mental hygiene with the “biocratic” ideas of this psychiatrist: to rationalise social and sexual life, through science. Even though this movement founded, an association, instigated a scholarly society, produced its own journal and was supported by eminent personalities of the medical, political and cultural worlds, there is very little historical trace of this movement and consequently it is generally unknown. The author of the article explores the reasons for this oversight and analyses the relationships between this movement and some members of the then emerging French psychoanalytical movement, particularly Dr. Paul Schiff and Princess Marie Bonaparte, from the beginning of 1930’s to the war. The author retraces the socio-political factors which underpinned Toulouse’s initiative and the causes of its apparent failure. This particular case also highlights the relationships between contemporary psychiatry, eugenics and psychoanalysis, and more generally between science, popularization and ideology. 相似文献
89.
André Breton Annick Bernalier Martine Dusser Gérard Fonty Brigitte Gaillard-Martinie Jean Guillot 《FEMS microbiology letters》1990,70(2):177-182
A new species of strictly anaerobic fungus was isolated from the cow rumen. It is characterized by a polycentric thallus, a polynuclear rhizomycelium, mucronate zoosporangia and uniflagellated zoospores. It is also singular in that the sporocysts do not react to the specific lectins of L-fucose, N-acetyl-D-galactosamine and diacetyl chitobiose. These characteristics justify the creation of a new genus. 相似文献
90.
Lucie Post Cécile Garnaud Danièle Maubon Hervé Pelloux Catherine Mansard Annick Bosseray Céline Dard 《Parasitology international》2018,67(1):1-3
Cystoisospora belli (previously known as Isospora belli) is a tropical coccidian parasite sometimes leading to severe diarrhea in immunocompromised patients. Here we describe a fatal case of cystoisosporiasis in a non HIV-immunocompromised 71-year-old female with no recent travel history. Infection was either latent or potentially caused by the consumption of contaminated imported food from Asia. Diagnosis was made by microscopical detection of numerous C. belli oocysts in stools without specific staining. Treatment with TMP-SMZ slightly improved diarrhea within 3 days, but dehydration subsequently led to acute decompensated heart failure and a fatal evolution. This report illustrates the possibility of severe cystoisosporiasis in non HIV-immunocompromised patients in a non-endemic country and highlights the risk of transmission through imported contaminated food consumption. 相似文献