The ability of air ions to produce changes in the electrical properties of L 1210 mouse leukemia cells was tested. The fluorescence of ANS incorporated into a membrane lipid bilayer (measured microphotometrically) was used as a probe. It was shown that the action of air ions of both signs could change (negative ions by increasing and the positive ones by decreasing) the fluorescence intensity of ANS in the cell surface structures or to an imbalance of ions inside and outside the cell. Both possibilities are discussed in the light of the results of experiments using ouabain or biguanide as factors diminishing the intensity of ANS fluorescence. 相似文献
Paleontological Journal - Foraminifer assemblages from five massifs of the Triassic limestones of the Dalnegorsk District have been described for the first time, and images of these foraminifers... 相似文献
The brown trout populations of the Baltic Sea region have been drastically affected by various human activities during the
past century. Due to their propensity to home to their natal site to spawn and their tendency to evolve local adaptations,
populations may be genetically differentiated in water systems where no physical barriers preventing interbreeding exist.
Consequently, identification of management units, a prerequisite for appropriate conservation and management planning, cannot
necessarily be deduced from the physical properties of the habitat. In this study, microsatellite markers were employed to
assess the spatio-temporal genetic structuring of inter-connected brown trout populations from a river-system in Northwest
Russia. Populations were found to be genetically differentiated from each other (global FST 0.06) and the genetic structuring within the river to follow an isolation by distance pattern. Indications of temporal stability
were found in some populations, however others appeared to be temporally unstable suggesting differences in the demographic
forces affecting the populations. Based on the observed isolation by distance pattern of genetic differentiation, preserving
several breeding sites spaced evenly throughout the river-system would appear to be more appropriate than focussing conservation
effort on any single stretch of the river.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
Fusarium head blight (FHB) is one of the most serious diseases in barley and wheat, as it is usually accompanied by the production of harmful mycotoxins in the grains. To identify FHB-resistant breeding resources, we evaluated 60 elite germplasm accessions of barley (24) and wheat (36) for FHB and mycotoxin accumulation. Assessments were performed in a greenhouse and five heads per accession were inoculated with both Fusarium asiaticum (Fa73, nivalenol producer) and F. graminearum (Fg39, deoxynivalenol producer) strains. While the accessions varied in disease severity and mycotoxin production, four wheat and one barley showed <20% FHB severity repeatedly by both strains. Mycotoxin levels in these accessions ranged up to 3.9 mg/kg. FHB severity was generally higher in barley than in wheat, and Fa73 was more aggressive in both crops than Fg39. Fg39 itself, however, was more aggressive toward wheat and produced more mycotoxin in wheat than in barley. FHB severity by Fa73 and Fg39 were moderately correlated in both crops (r = 0.57/0.60 in barley and 0.42/0.58 in wheat). FHB severity and toxin production were also correlated in both crops, with a stronger correlation for Fa73 (r = 0.42/0.82 in barley, 0.70 in wheat) than for Fg39. 相似文献
An aerobic, gram-stain-negative, pink-colored, non-motile and rod-shaped algicidal bacterium, designated as JA-25T was isolated from freshwater in Geumgang River, Republic of Korea. Strain JA-25T grew at 15–30 °C and pH 6–9, and did not require NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain JA-25T belongs to the family ‘Spirosomaceae’ and is most closely related to Fibrella aestuarina BUZ 2T (93.6%). Strain JA-25T showed?<?90% sequence similarity to other members of the family ‘Spirosomaceae’. The average nucleotide identity(ANI), in silico DNA-DNA hybridization and average amino acid identity(AAI) values based on the genomic sequences of JA-25T and F. aestuarina BUZ 2T were 74.4, 20.5, and 73.6%, respectively. Strain JA-25T showed an algicidal effect on the marine flagellate alga Heterocapsa triquetra, but no effect on fresh water cyanobacterium (Nostoc). In genome analysis, RIPP-like peptides were detected and predicted to resemble the indolmycin biosynthetic gene cluster, which possibly influence its algicidal effect. Furthermore, a bacteriorhodopsin gene with photoheterotrophic characteristics was detected. The genomic DNA G?+?C content was 52.5 mol%. The major cellular fatty acids were summed feature 3 (C16:1 ω6c/C16:1 ω7c), C16:1 ω5c, C16:0 (>?10%). The major respiratory quinone was menaquinone 7 and major polar lipids were phosphatidylethanolamine, two unidentified aminolipids, two phospholipids, and five unidentified lipids. Considering the phylogenetic inference, phenotypic, and chemotaxonomic data, strain JA-25T should be classified as a novel species in the novel genus Fibrivirga, with the proposed name Fibrivirga algicola sp. nov. The type strain is JA-25T (=?KCCM 43334T?=?NBRC 114259T).
This paper considers the clustering problem of physical step count data recorded on wearable devices. Clustering step data give an insight into an individual's activity status and further provide the groundwork for health‐related policies. However, classical methods, such as K‐means clustering and hierarchical clustering, are not suitable for step count data that are typically high‐dimensional and zero‐inflated. This paper presents a new clustering method for step data based on a novel combination of ensemble clustering and binning. We first construct multiple sets of binned data by changing the size and starting position of the bin, and then merge the clustering results from the binned data using a voting method. The advantage of binning, as a critical component, is that it substantially reduces the dimension of the original data while preserving the essential characteristics of the data. As a result, combining clustering results from multiple binned data can provide an improved clustering result that reflects both local and global structures of the data. Simulation studies and real data analysis were carried out to evaluate the empirical performance of the proposed method and demonstrate its general utility. 相似文献
Effects of fusaproliferin (FUS) on membrane potential (EM), electrolyte leakage, enzymes activity and respiration of roots, were studied in two maize cultivars (Zea mays L.), differing in their susceptibility to this toxin. In short-term experiments (≤ 6 h), EM has been rapidly and significantly depolarized by FUS. The rapidity of EM depolarization in tolerant cv. Lucia was more expressive in comparison with susceptible cv. Pavla, but the extent of EM depolarization was higher in cv. Pavla. In both maize cultivars, higher depolarization of EM was registered in cells of root zone I. In long-term experiments after the first EM depolarization, which occurred during the first 6 h of FUS treatment, gradual depolarization continued up to 24 h and was
represented not only by the active component (EP) but also by the passive component (ED) of EM. The decrease in EM and ED was followed by a loss of K+ ions from FUS treated roots of both cultivars. The leak of K+ ions from the root cells of both root zones as well as both maize cultivars increased with the time of FUS treatment and
was significantly higher in susceptible cv. Pavla than in tolerant cv. Lucia. FUS treatment of maize roots resulted in a significant
decrease of root respiration which was higher in susceptible cv. Pavla than in tolerant cv. Lucia. 相似文献