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81.
This paper describes a protocol to develop cotton mutant lines with improved yield and fiber characters. Immature ovules [(15-day
post-anthesis ovules (dap)] were irradiated with 10–50 Gy gamma rays and treated with 1–5 mM EMS and SA to investigate the
stimulatory effects of mutagenic treatments. During the subsequent field trials, the mutant lines showed significant variations
from control lines. Lower dose/concentration of mutagenic treatments effectively stimulate the agronomical characters like
early flowering, plant height, number of bolls, yield of seed cotton, ginning percent, seed index, harvest index and fiber
characters while exposure at higher dose/concentration results in lowering the value of the parameters. Consequently, we use
this approach to induce genetic variability for obtaining novel mutant cotton cultivars. Among the 19 different mutant lines
isolated from the study, M7 showed higher morphological variations in terms of yield characters such as plant height, number
of bolls, yield of seed cotton, ginning percent and fiber characters. Significant increase in cellulose content was also noted
in mutant lines, whereas moderate increase was observed in total fiber units of the mutant lines. The selected cotton mutant
lines for cultivars were investigated systematically; these lines significantly increased the potential for agronomical enhancement
of cotton yield. 相似文献
82.
Qin J Dhondi P Huang X Mandal M Zhao Z Pissarnitski D Zhou W Aslanian R Zhu Z Greenlee W Clader J Zhang L Cohen-Williams M Jones N Hyde L Palani A 《Bioorganic & medicinal chemistry letters》2011,21(2):664-669
We herein report the discovery of four series of fused 5,6-bicyclic heterocycles as γ-secretase modulators. Synthesis and SAR of these series are discussed. These compounds represent a new class of γ-secretase modulators that demonstrate moderate to good in vitro potency in inhibiting Aβ42 production. 相似文献
83.
Anandan SK Webb HK Chen D Wang YX Aavula BR Cases S Cheng Y Do ZN Mehra U Tran V Vincelette J Waszczuk J White K Wong KR Zhang LN Jones PD Hammock BD Patel DV Whitcomb R MacIntyre DE Sabry J Gless R 《Bioorganic & medicinal chemistry letters》2011,21(3):983-988
1-(1-Acetyl-piperidin-4-yl)-3-adamantan-1-yl-urea 14a (AR9281), a potent and selective soluble epoxide hydrolase inhibitor, was recently tested in a phase 2a clinical setting for its effectiveness in reducing blood pressure and improving insulin resistance in pre-diabetic patients. In a mouse model of diet induced obesity, AR9281 attenuated the enhanced glucose excursion following an intraperitoneal glucose tolerance test. AR9281 also attenuated the increase in blood pressure in angiotensin-II-induced hypertension in rats. These effects were dose-dependent and well correlated with inhibition of the sEH activity in whole blood, consistent with a role of sEH in the observed pharmacology in rodents. 相似文献
84.
Jacob Jobin John Rachel Tanya Shankar Baby Abirami Gunasekaran Karthik Iyadurai Ramya Anandan Shalini Veeraraghavan Balaji 《Molecular biology reports》2020,47(10):7797-7803
Molecular Biology Reports - Traditional serotyping based on the phenotypic variation of O- and H-antigen remains as the gold-standard for the identification and classification of Salmonella... 相似文献
85.
Susannah Piek Zhirui Wang Jhuma Ganguly Adam M. Lakey Stephanie N. Bartley Shakeel Mowlaboccus Anandhi Anandan Keith A. Stubbs Martin J. Scanlon Alice Vrielink Parastoo Azadi Russell W. Carlson Charlene M. Kahler 《PloS one》2014,9(9)
The decoration of the lipid A headgroups of the lipooligosaccharide (LOS) by the LOS phosphoethanolamine (PEA) transferase (LptA) in Neisseria spp. is central for resistance to polymyxin. The structure of the globular domain of LptA shows that the protein has five disulphide bonds, indicating that it is a potential substrate of the protein oxidation pathway in the bacterial periplasm. When neisserial LptA was expressed in Escherichia coli in the presence of the oxidoreductase, EcDsbA, polymyxin resistance increased 30-fold. LptA decorated one position of the E. coli lipid A headgroups with PEA. In the absence of the EcDsbA, LptA was degraded in E. coli. Neisseria spp. express three oxidoreductases, DsbA1, DsbA2 and DsbA3, each of which appear to donate disulphide bonds to different targets. Inactivation of each oxidoreductase in N. meningitidis enhanced sensitivity to polymyxin with combinatorial mutants displaying an additive increase in sensitivity to polymyxin, indicating that the oxidoreductases were required for multiple pathways leading to polymyxin resistance. Correlates were sought between polymyxin sensitivity, LptA stability or activity and the presence of each of the neisserial oxidoreductases. Only meningococcal mutants lacking DsbA3 had a measurable decrease in the amount of PEA decoration on lipid A headgroups implying that LptA stability was supported by the presence of DsbA3 but did not require DsbA1/2 even though these oxidoreductases could oxidise the protein. This is the first indication that DsbA3 acts as an oxidoreductase in vivo and that multiple oxidoreductases may be involved in oxidising the one target in N. meningitidis. In conclusion, LptA is stabilised by disulphide bonds within the protein. This effect was more pronounced when neisserial LptA was expressed in E. coli than in N. meningitidis and may reflect that other factors in the neisserial periplasm have a role in LptA stability. 相似文献
86.
Biological evaluation and interconversion studies of rotamers of SCH 351125, an orally bioavailable CCR5 antagonist 总被引:1,自引:0,他引:1
Palani A Shapiro S Clader JW Greenlee WJ Blythin D Cox K Wagner NE Strizki J Baroudy BM Dan N 《Bioorganic & medicinal chemistry letters》2003,13(4):705-708
The separation and biological evaluation of rotamers as well as interconversion studies on rotamers of our clinical candidate SCH 351125 are described. 相似文献
87.
Anupriya G Roopa K Basappa S Chong YS Annamalai L 《Journal of molecular modeling》2011,17(12):3325-3332
The three-dimensional (3D) structure of the substrate binding domain (SBD) of human ubiquitin ligase Siah2 (seven in absentia
homolog) was constructed based on the homology modeling approach using the Modeller 9v7 program. The molecular dynamics method
was utilized to refine the model and it was further assessed by ProSA, three-dimensional structural superposition (3d-SS)
and PROCHEK in order to analyze the quality and reliability of the generated model. Furthermore, we predicted the binding
pocket of Siah2 and also validated it by both blind and normal docking using a known functional inhibitor, menadione. Using
structure-based high-throughput virtual screening, we identified five lead drug-like molecules against the modeled SBD of
Siah2 and analyzed its pharmacokinetic properties to identify the potential inhibitors for Siah2. The docking results for
menadione and the lead molecules at the ligand binding site of SBD of Siah2 revealed that the residue Ser39 (corresponding
to Ser167 in the full-length protein) is consistently involved in strong hydrogen bonding, and plays an important role in
phosphorylation and the enhanced activity of Siah2. 相似文献
88.
Ananda Anandan Laurence N. Gatehouse Richelle K. Marshall Colleen Murray John T. Christeller 《Plant Molecular Biology Reporter》2009,27(3):355-364
Two variants of the promoter of the squash aspartic acid protease inhibitor multigene family were isolated from Cucurbita maxima cv. ‘Supermarket’ Hybrid genomic DNA. The isolated promoters, possibly not full length, comprised a 5′-untranslated region
(UTR) of 202–208 bp, contained a 63-bp upstream open reading frame (uORF) and the immediate upstream sequences of 441–445 bp.
The two promoters contained several small deletions relative to each other and 22 single base differences but exhibit overall
92.5% homology over 654 bp. When the promoters were fused to a β-glucuronidase reporter gene and expressed in tobacco, one
variant was highly expressed in the companion cells of the inner and outer phloem of leaves and at lower levels in other organs.
The other variant was expressed at high levels in the long glandular trichomes of the leaf. Deletion analysis identified a
region of ~280 bp immediately upstream of the 5′-UTR containing the TATA box that was responsible for phloem specific expression
and a further region of ~180 bp that enhanced expression in one promoter and conferred trichome expression in the other. Removal
of the 5′-UTR, including the uORF, inactivated the phloem promoter. 相似文献
89.
Buddhan S Sivakumar R Dhandapani N Ganesan B Anandan R 《Prostaglandins, leukotrienes, and essential fatty acids》2007,76(6):349-355
Mitochondria are an important intracellular source and target of reactive oxygen species. The life span of a species is thought to be determined, in part, by the rate of mitochondrial damage inflicted by oxygen free radicals during the course of normal cellular metabolism. In the present study, we have investigated the protective effect of squalene supplementation for 15 days and 30 days on energy status and antioxidant defense system in liver mitochondria of 18 young and 18 aged rats. The dietary supplementation of 2% squalene significantly minimized aging associated alterations in mitochondrial energy status by maintaining the activities of TCA cycle enzymes (isocitrate dehydrogenase, α-ketoglutarate dehydrogenase, succinate dehydrogenase and malate dehydrogenase) and respiratory marker enzymes (NADH dehydrogenase and cytochrome-c-oxidase) at higher level in the liver mitochondria of aged rats compared with unsupplemented controls. It exerted an antioxidant effect by inhibiting mitochondrial lipid peroxidation (malondialdehyde) in liver of young and aged rats. Supplementation with squalene also maintained the mitochondrial antioxidant defense system at higher rate by increasing the level of reduced glutathione and the activities of glutathione-dependent antioxidant enzymes (glutathione peroxidase and glutathione-S-transferase) and antiperoxidative enzymes (superoxide dismutase and catalase) in the liver of young and aged rats. The results of this study provide evidence that dietary supplementation with squalene can improve liver mitochondrial function during aging and minimize the age-associated disorders in which reactive oxygen species are a major cause. 相似文献
90.
Divya K. Vijayan P. R. Sreerekha Pavan Kumar Dara B. Ganesan Suseela Mathew Rangasamy Anandan C. N. Ravisankar 《Cell stress & chaperones》2022,27(1):45
The aim of the present study was to investigate the ability of fish collagen peptides (FCP) from the skin of great hammerhead shark (Sphyrna mokarran) to avert the occurrence of gastric ulcer in experimental rats. FCP treatment prevented the formation of ulcerative lesions on gastric tissues with 86% of inhibition. The histopathology analysis of gastric tissue revealed that the FCP intake prevented the occurrence of hemorrhage and erosion in gastric tissue with formation of mild edema and necrosis, as well as normalized the pH and volume of gastric juice. It also downregulated the expression of pro-inflammatory marker interferon-ɤ (IFN-ɤ) and upregulated the anti-inflammatory marker interleukin-4 (IL-4) in gastric tissue. FCP is capable to modulate the oxidative stress by enhancing the activity of antioxidant defense enzymes superoxide dismutase (SOD) and catalase and by lowering the levels of membrane lipid peroxidation. 相似文献