排序方式: 共有38条查询结果,搜索用时 15 毫秒
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Journal of Applied Phycology - An experimental farm has been installed in the Kiel Fjord, western Baltic Sea, aiming at the development of a sustainable production process for Fucus species (Fucus... 相似文献
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Karlo Komorowski Annalena Salditt Yihui Xu Halenur Yavuz Martha Brennich Reinhard Jahn Tim Salditt 《Biophysical journal》2018,114(8):1908-1920
We have studied the adhesion state (also denoted by docking state) of lipid vesicles as induced by the divalent ions Ca2+ or Mg2+ at well-controlled ion concentration, lipid composition, and charge density. The bilayer structure and the interbilayer distance in the docking state were analyzed by small-angle x-ray scattering. A strong adhesion state was observed for DOPC:DOPS vesicles, indicating like-charge attraction resulting from ion correlations. The observed interbilayer separations of ~1.6 nm agree quantitatively with the predictions of electrostatics in the strong coupling regime. Although this phenomenon was observed when mixing anionic and zwitterionic (or neutral) lipids, pure anionic membranes (DOPS) with highest charge density σ resulted in a direct phase transition to a multilamellar state, which must be accompanied by rupture and fusion of vesicles. To extend the structural assay toward protein-controlled docking and fusion, we have characterized reconstituted N-ethylmaleimide-sensitive factor attachment protein receptors in controlled proteoliposome suspensions by small-angle x-ray scattering. 相似文献
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Lech Kaczmarczyk Melvin Schleif Lars Dittrich Rhiannan H. Williams Marua Koderman Vikas Bansal Ashish Rajput Theresa Schulte Maria Jonson Clemens Krost Fabio J. Testaquadra Stefan Bonn Walker S. Jackson 《PLoS pathogens》2022,18(8)
Selective vulnerability is an enigmatic feature of neurodegenerative diseases (NDs), whereby a widely expressed protein causes lesions in specific cell types and brain regions. Using the RiboTag method in mice, translational responses of five neural subtypes to acquired prion disease (PrD) were measured. Pre-onset and disease onset timepoints were chosen based on longitudinal electroencephalography (EEG) that revealed a gradual increase in theta power between 10- and 18-weeks after prion injection, resembling a clinical feature of human PrD. At disease onset, marked by significantly increased theta power and histopathological lesions, mice had pronounced translatome changes in all five cell types despite appearing normal. Remarkably, at a pre-onset stage, prior to EEG and neuropathological changes, we found that 1) translatomes of astrocytes indicated reduced synthesis of ribosomal and mitochondrial components, 2) glutamatergic neurons showed increased expression of cytoskeletal genes, and 3) GABAergic neurons revealed reduced expression of circadian rhythm genes. These data demonstrate that early translatome responses to neurodegeneration emerge prior to conventional markers of disease and are cell type-specific. Therapeutic strategies may need to target multiple pathways in specific populations of cells, early in disease. 相似文献
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Johannes M. Wagner Annalena Wille Maria Fueth Sarah Weske Sebastian Lotzien Felix Reinkemeier Christoph Wallner Alexander Sogorski Stephanie Dittfeld Mustafa Becerikli Thomas A. Schildhauer Marcus Lehnhardt Bodo Levkau Björn Behr 《Journal of cellular and molecular medicine》2023,27(23):3786-3795
Posttraumatic osteomyelitis and the ensuing bone defects are a debilitating complication after open fractures with little therapeutic options. We have recently identified potent osteoanabolic effects of sphingosine-1-phosphate (S1P) signalling and have now tested whether it may beneficially affect bone regeneration after infection. We employed pharmacological S1P lyase inhibition by 4-deoxypyrodoxin (DOP) to raise S1P levels in vivo in an unicortical long bone defect model of posttraumatic osteomyelitis in mice. In a translational approach, human bone specimens of clinical osteomyelitis patients were treated in organ culture in vitro with DOP. Bone regeneration was assessed by μCT, histomorphometry, immunohistology and gene expression analysis. The role of S1P receptors was addressed using S1PR3 deficient mice. Here, we present data that DOP treatment markedly enhanced osteogenesis in posttraumatic osteomyelitis. This was accompanied by greatly improved osteoblastogenesis and enhanced angiogenesis in the callus accompanied by osteoclast-mediated bone remodelling. We also identified the target of increased S1P to be the S1PR3 as S1PR3−/− mice showed no improvement of bone regeneration by DOP. In the human bone explants, bone mass significantly increased along with enhanced osteoblastogenesis and angiogenesis. Our data suggest that enhancement of S1P/S1PR3 signalling may be a promising therapeutic target for bone regeneration in posttraumatic osteomyelitis. 相似文献
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G. Loudianos Valeria Dessì Andrea Angius Mario Lovicu Angela Loi Manila Deiana Nejat Akar Pietro Vajro Annalena Figus Antonio Cao Mario Pirastu 《Human genetics》1996,98(6):640-642
This study reports 12 novel mutations of the Wilson disease (WD) gene which have been detected by the molecular analysis
of 29 patients of Mediterranean descent carrying uncommon chromosomal haplotypes at the WD locus. These mutations include
two nonsense, one splice site and nine missense. The missense mutations lie in regions of the WD gene critical for its function,
such as the transmembrane region, the transduction domain and the ATP loop and ATP-binding domain, indicating that they are
disease-causing mutations. These new findings improve our knowledge for the role played by functional domains on the ATP7B
function.
Received: 20 March 1996 相似文献