全文获取类型
收费全文 | 21921篇 |
免费 | 1497篇 |
国内免费 | 9篇 |
专业分类
23427篇 |
出版年
2024年 | 19篇 |
2023年 | 126篇 |
2022年 | 311篇 |
2021年 | 580篇 |
2020年 | 351篇 |
2019年 | 470篇 |
2018年 | 593篇 |
2017年 | 478篇 |
2016年 | 809篇 |
2015年 | 1215篇 |
2014年 | 1343篇 |
2013年 | 1659篇 |
2012年 | 1948篇 |
2011年 | 1892篇 |
2010年 | 1174篇 |
2009年 | 1016篇 |
2008年 | 1376篇 |
2007年 | 1287篇 |
2006年 | 1179篇 |
2005年 | 1081篇 |
2004年 | 981篇 |
2003年 | 927篇 |
2002年 | 751篇 |
2001年 | 117篇 |
2000年 | 106篇 |
1999年 | 135篇 |
1998年 | 125篇 |
1997年 | 115篇 |
1996年 | 89篇 |
1995年 | 86篇 |
1994年 | 90篇 |
1993年 | 88篇 |
1992年 | 53篇 |
1991年 | 56篇 |
1990年 | 63篇 |
1989年 | 45篇 |
1988年 | 50篇 |
1987年 | 38篇 |
1986年 | 42篇 |
1985年 | 51篇 |
1984年 | 39篇 |
1983年 | 44篇 |
1982年 | 32篇 |
1981年 | 42篇 |
1980年 | 26篇 |
1979年 | 34篇 |
1978年 | 32篇 |
1977年 | 29篇 |
1976年 | 25篇 |
1975年 | 22篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
131.
132.
Multiple components in two antigenic variants of staphylococcal exfoliative toxin were demonstrated by isoelectric focusing in polyacrylamide gel. The main components had an isoionic point of pH=7.0 while other components ranged from pI=4.5 to pI=8.5. Each individual component was shown to possess full biologic and serologic activity after focusing. Isoelectric focusing in the presence of 8 M urea also showed microheterogeneity in exfoliative toxin preparations. The various components appear to be reversible conformers, possibly raised by exposure to the ph gradient of the ampholines. 相似文献
133.
Biochemical changes of rat brain membranes with aging 总被引:4,自引:0,他引:4
Gabriella Calderini Anna Chiara Bonetti Antonio Battistella Fulton Timm Crews Gino Toffano 《Neurochemical research》1983,8(4):483-492
Modification of membrane composition and enzymatic activities both in total brain homogenate and purified synaptic plasma membrane of 3 and 24 month old rats has been investigated. Protein, cholesterol and phospholipid content and (Na+, K+)ATPase and 2',3' cyclic nucleotide phosphohydrolase activities were determined. The major changes occurred in the whole homogenate where a general increase in total protein and cholesterol content with age and a significant increase of the cholesterol/phospholipids molar ratio has been detected. In S.P.M. aging process induced a decrease of protein, cholesterol and phospholipids content associated with an increased membrane viscosity and a decrease of delta E. These data are consistent with a change in the structural organization and in the distribution pattern of different cell population in the aging brain. A possible artifactual effect of freezing on the reported parameter is also discussed. 相似文献
134.
Anna Chrtková 《Folia Geobotanica》1983,18(1):103-105
A new subspecies,Lathyrus nissolia L. subsp.futakii Chrtková subsp. nova is described from East Slovakia. The diacritical characters are: high and rich branched stems, 50–110 cm long and larger, 10–12 mm long, bright orange-red flowers. It differs also in ecology, growing in wet lowland forests with the level of the ground water up to 15 cm over the earth also in summer. 相似文献
135.
136.
Orsola Tiboni Rita Cantoni Roberta Creti Piero Cammarano Anna Maria Sanangelantoni 《Journal of molecular evolution》1991,33(2):142-151
Summary The gene (fus) coding for elongation factor G (EF-G) of the extremely thermophilic eubacteriumThermotoga maritima was identified and sequenced. The EF-G coding sequence (2046 bp) was found to lie in an operon-like structure between the ribosomal protein S7 gene (rpsG) and the elongation factor Tu (EF-Tu) gene (tuf). TherpsG, fus, andtuf genes follow each other immediately in that order, which corresponds to the order of the homologous genes in thestr operon ofEscherichia coli. The derived amino acid sequence of the EF-G protein (682 residues) was aligned with the homologous sequences of other eubacteria, eukaryotes (hamster), and archaebacteria (Methanococcus vannielii). Unrooted phylogenetic dendrogram, obtained both from the amino acid and the nucleotide sequence alignments, using a variety of methods, lend further support to the notion that the (present) root of the (eu)bacterial tree lies betweenThermotoga and the other bacterial lineages. 相似文献
137.
Anna Krahulcová 《Folia Geobotanica》1991,26(4):369-377
Chromosome numbers compared with as yet published data are given for the following 12 Phanerogams (both native species and aliens) from Czechoslovakia:Ambrosia trifida L.,Cardamine chelidonia L.,Dephne cneorum L.,Epipactis albensis Nováková etRydlo,Linum flavum L.subsp flavum, Lunaria rediviva L.,Nepeta grandiflora M.BIEB.,Reseda luteola L.,Thlaspi montanum L.,Tithymalus salicifolius (Host)Klotzsch etGarcke,Tithymalus virgultosus (Klokov) Holub andVerbascum speciosum Schrad. subsp.speciosum. The chromosome number 2n=40 is presented for the first time in autogamousEpipactis albensis Nováková etRydlo. New chromosome numbers were found inCardamine chelidonia L. (2n=32) and inTithymalus salicifolius (Host) Klotzch etGarcke (2n=40). Known but less frequent cytotypes are reported inLinum flavum L. subsp.flavum (2n=28) and inVerbascum speciosum Schrad. subsp.speciosum (2n=30). 相似文献
138.
Pamela Manzi Giovannella Bruscalupi Flavia Castellano Anna Trentalance 《Bioscience reports》1992,12(3):215-219
In female frogs (Rana Esculenta) during gametogenesis the cholesterol synthesized in the liver by 3-hydroxy-3-methylglutaryl coenzyme A reductase is mostly exported into the blood and taken up by the oocytes.In order to understand the fate of the neosynthesized cholesterol, female and male frogs and estrogenized male controls were injected with the labelled precursor14C mevalonate.In females and in estrogenized controls, mevalonate-derived radioactivity is found in a plasmatic lipoprotein that has been identified as vitellogenin by immunological detection.The increased 3-hydroxy-3-methylglutaryl coenzyme A reductase activity present in females in Fall is likely to be committed to provide cholesterol for the lipidation of this cholesterol-rich protein. 相似文献
139.
140.