Lathyrus nissolia subsp.futakii subsp. nova in der Tschechoslowakei

A new subspecies,Lathyrus nissolia L. subsp.futakii Chrtková subsp. nova is described from East Slovakia. The diacritical characters are: high and rich branched stems, 50–110 cm long and larger, 10–12 mm long, bright orange-red flowers. It differs also in ecology, growing in wet lowland forests with the level of the ground water up to 15 cm over the earth also in summer.     

Selected chromosome counts of the Czechoslovak flora III

Chromosome numbers compared with as yet published data are given for the following 12 Phanerogams (both native species and aliens) from Czechoslovakia:Ambrosia trifida L.,Cardamine chelidonia L.,Dephne cneorum L.,Epipactis albensis Nováková etRydlo,Linum flavum L.subsp flavum, Lunaria rediviva L.,Nepeta grandiflora M.BIEB.,Reseda luteola L.,Thlaspi montanum L.,Tithymalus salicifolius (Host)Klotzsch etGarcke,Tithymalus virgultosus (Klokov) Holub andVerbascum speciosum Schrad. subsp.speciosum. The chromosome number 2n=40 is presented for the first time in autogamousEpipactis albensis Nováková etRydlo. New chromosome numbers were found inCardamine chelidonia L. (2n=32) and inTithymalus salicifolius (Host) Klotzch etGarcke (2n=40). Known but less frequent cytotypes are reported inLinum flavum L. subsp.flavum (2n=28) and inVerbascum speciosum Schrad. subsp.speciosum (2n=30).     

The vitelline envelope,chorion, and micropyle of Fundulus heteroclitus eggs

The architecture and transformation of the vitelline envelope of the developing oocyte into the chorion of the mature egg of Fundulus heteroclitus have been examined by scanning and transmission electron microscopy. The mature vitelline envelope is structurally complex and consists of about nine strata. The envelope is penetrated by pore canals that contain microvilli arising from the oocyte and macrovilli from follicle cells. During the envelope's transformation into the chorion, the pore canals are lost and the envelope becomes more fibrous and compact and its stratified nature less apparent. The micropyle, of pore, through which the sperm gains access to the enclosed egg is located at the bottom of a small funnel-shaped depression in the envelope. Internally, the micropyle opens on the apex of a cone-like elevation of the chorion. During the development of the envelope, structured chorionic fibrils, the components of which are presumed to be synthesized by the follicle cells, become attached to its surface. These chorionic fibrils are though to aid in the attachment of the egg to the substratum and perhaps to help prevent water loss during low tides when the egg may be exposed.     

High resolution two-dimensional gel electrophoresis of the proteins and glycoproteins of human blood platelets and platelet membranes

The proteins and glycoproteins of human blood platelets and platelet membranes in both the reduced and the unreduced states have been analysed by isoelectric focusing and sodium dodecyl sulphate-discontinuous polyacrylamide gel electrophoresis in a two-dimensional technique. Gels which had been stained with periodic acid-Schiff's reagent could be counter-stained with Coomassie Brilliant Blue, simplifying the recognition of components which stain with both reagents. The major glycoproteins and some of the proteins have been identified and the characteristics of the membrane and of the whole platelet components established in this system.     

Uptake of immune RNA by normal mouse spleen cells

Summary Normal mouse spleen cells take up in vitro radioactively labeled immune RNA. RNA taken up is present in nuclei, polysomes, membranes and cytoplasm. About 20–40% of immune RNA is nonspecifically associated with cell surface. 45% of RNA taken up is degraded and reutilized inside the cells within 2 hours.This work was supported by the Polish Academy of Sciences within the project 09.7.4.1.1.     

Solubilization of microsomal phosphoethanolaminetransferase by octyl glucoside

Phosphoethanolaminetransferase of high specific activity was solubilized from rat liver microsomes with the non-ionic detergent octyl glucoside. The solubilization method is fast and simple, allowing for processing of large amounts of material. The solubilized enzyme is stable. It contains virtually no phosphocholinetransferase activity. A preliminary characterization of the enzyme, with both diacyl- and alkylacyl-glycerol as substrate, is given. For the reaction, the lipid substrates were incorporated into artificial phospholipid bilayers (liposomes).     

Next-Generation Sequencing of Connective Tissue Genes in Patients with Classical Ehlers-Danlos Syndrome

Background: Ehlers-Danlos syndrome (EDS) is a common non-inflammatory, congenital connective tissue disorder. Classical type (cEDS) EDS is one of the more common forms, typically caused by mutations in the COL5A1 and COL5A2 genes, though causative mutations in the COL1A1 gene have also been described. Material and methods: The study group included 59 patients of Polish origin, diagnosed with cEDS. The analysis was performed on genomic DNA (gDNA) with NGS technology, using an Illumina sequencer. Thirty-five genes related to connective tissue were investigated. The pathogenicity of the detected variants was assessed by VarSome. Results: The NGS of 35 genes revealed variants within the COL5A1, COL5A2, COL1A1, and COL1A2 genes for 30 of the 59 patients investigated. Our panel detected no sequence variations for the remaining 29 patients. Discussion: Next-generation sequencing, with an appropriate multigene panel, showed great potential to assist in the diagnosis of EDS and other connective tissue disorders. Our data also show that not all causative genes giving rise to cEDS have been elucidated yet.     

Insights into the feeding ecology of the Seychelles Black Parrot Coracopsis barklyi using two monitoring approaches

Feeding ecology is an important factor for the survival of a species and knowledge of its parameters is a prerequisite for successful conservation work. In this study we describe the feeding ecology of the endemic Seychelles Black Parrot Coracopsis barklyi on Praslin, Seychelles, the only island on which this parrot is resident. We compared two methods to evaluate feeding choices: incidental observations and feeding walks on 25 transects in all habitat types. Black parrots fed on 46 different species, bringing the total number of known food plants to 53 species. They predominantly consumed endemic and native species (58% of observed feeding bouts), mainly their fruit pulp (in 68% of feeding bouts), followed by buds (15%) and seeds (37%) with occasional observations of leaves, bark and scale insects. The incidental method rendered many more observed bouts than the transect approach and the ratios of consumed species differed between methods but the transect results are regarded as more representative. The incidental method is not suitable for quantitative conclusions but complements the transect method, providing information about rarely occurring feeding events.