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101.
Epithelial M cells in the rabbit caecal lymphoid patch display distinctive surface characteristics 总被引:5,自引:0,他引:5
Mark A. Jepson M. Ann Clark Nicholas L. Simmons Barry H. Hirst 《Histochemistry and cell biology》1993,100(6):441-447
The follicle-associated epithelium (FAE) in the rabbit caecal lymphoid patch is characterised by the presence of membranous (M) cells, which are believed to be functionally equivalent to those present at other sites of gut-associated lymphoid tissue (GALT). Caecal patch M cells display distinctive features compared with those of other GALT sites, despite similar general morphology and expression of the M cell marker vimentin, suggesting marked heterogeneity in the apical surface of M cells at discrete GALT sites. Electron microscopy reveals that rabbit caecal patch M cells differ from those in the small intestinal Peyer's patch FAE: the former have a prominent aspect within the epithelium and possess microvilli which are longer than those of adjacent enterocytes. Many of the M cells in peripheral regions of the caecal patch FAE are not associated with leucocytes and may thus represent an immature M cell population. The M cells are also histochemically distinct from adjacent enterocytes and from Peyer's patch M cells, showing greater expression of brush-border alkaline phosphatase activity and affinity for certain lectins (peanut and wheat germ agglutinins, Bandeiraea simplicifolia agglutinin II). The differences in the brush-border morphology and glycocalyx structure between M cells at different GALT sites may affect their function at these sites by influencing the interaction of luminal antigens and microorganisms with the M cell surface. The present data also support the hypothesis that M cells arise directly from differentiation of crypt stem cells and not from the transformation of existing fully differentiated enterocytes. 相似文献
102.
Abstract The thymus of the sole Solea solea contained lymphoblasts and thymocytes within a network of pale and dark epithelial cells. The pale cells were characterized by tonofilaments and desmosomes and some embraced rodlet cells within their cytoplasmic processes. The dark epithelial cells had numerous electron-dense inclusions and electron-lucent vacuoles. Lymphocytes were closely associated with the plasma membrane of both types of epithelial cells and with macrophages. Breakdown of effete lymphocytes appeared to be the main function of the macrophages. Some macrophages were multinucleated. Those containing melanin granules associated with phagosomes were classified as melanomacrophages. Pigment cells including melanophores and guanophores were present along the connective tissue trabeculae and surrounding the blood vessels. A few plasma cells and mucous cells were present. 相似文献
103.
The importance of ecological factors such as sex lability, spatial segregation, and resource allocation in the evolution of dioecy were examined in Schiedea globosa. S. globosa is a subdioecious species with equal numbers of plants possessing strictly male or female function and a small proportion of hermaphrodites. The propensity for labile sex expression was under both environmental and genetic control; some plants with male function became hermaphroditic (by producing female flowers) under better growing conditions in the field and in the greenhouse. There was some spatial segregation of the sexes. Because of sex lability, more hermaphrodites than males occurred on moister slopes. Although there were not measurable sex-related differences in mortality within or between two flowering seasons, more females than males and hermaphrodites occurred at the bottom of slopes. Males and females produced the same number of ramets and inflorescences, but females had a greater number of flowers per inflorescence. Males and females had the same number of ovules (vestigial in males), but females had larger ovules and longer stigmas. Hermaphrodites and males had the same amount of pollen per flower despite the production of fruit by the hermaphrodites. In hermaphrodites, there was no apparent tradeoff within flowers between pollen production and ovule production. These results indicate that spatial segregation, sex lability, and environmental conditions influence allocation patterns of S. globosa, and in combination with high inbreeding depression and selling rates, may promote the further evolution of dioecy in S. globosa. 相似文献
104.
Victoria G. Thayer Andrew J. Read Ari S. Friedlaender David R. Colby Aleta A. Hohn William A. McLellan D. Ann Pabst Jennifer L. Dearolf Nan I. Bowles John R. Russell Keith A. Rittmaster 《Marine Mammal Science》2003,19(4):617-629
We describe reproductive seasonality of bottlenose dolphins in North Carolina (NC), U.S.A., using strandings data from the entire coast of NC and sighting data from Beaufort, NC and by estimating dates of birth of known females. We found a strong peak of neonate strandings in the spring (April-May), and low levels of neonate strandings in the fall and winter. The distribution of neonate strandings was significantly different from a uniform distribution ( P < 0.001, K = 3.8). We found a unimodal distribution of 282 sightings of neonates with a diffuse peak in the summer. The temporal distribution of sightings of neonates departed significantly from a uniform distribution (P < 0.001, K = 5.1). Estimated birth dates of neonates from known females occurred in May ( n = 6) and June ( n = 4), with a single fall birth. These methods shed light on bottlenose reproductive patterns and underscore the value of using information from multiple types of data. Clarification of bottlenose dolphin reproductive patterns, such as the seasonality of birth, may enhance our understanding of the population structure of this species in the mid-Atlantic region. 相似文献
105.
106.
107.
Anthony L. Moore Ann L. Umbach James N. Siedow 《Journal of bioenergetics and biomembranes》1995,27(4):367-377
A major characteristic of plant mitochondria is the presence of a cyanide-insensitive alternative oxidase which catalyzes the reduction of oxygen to water. Current information on the properties of the oxidase is reviewed. Conserved amino acid motifs have been identified which suggest the presence of a hydroxo-bridged di-iron center in the active site of the alternative oxidase. On the basis of sequence comparison with other di-iron center proteins, a structural model for the active site of the alternative oxidase has been developed that has strong similarity to that of methane monoxygenase. Evidence is presented to suggest that the alternative oxidase of plant mitochondria is the newest member of the class II group of di-iron center proteins. 相似文献
108.
109.
Cytokinins are plant growth regulators that induce shoot formation, inhibit senescence and root growth. Experiments with hydroponically grown tobacco plants, however, indicated that exogenously applied cytokinin led to the accumulation of proline and osmotin. These responses were also associated with environmental stress reactions, such as salt stress, in many plant species. To test whether increased endogenous cytokinin accumulation led to NaCl stress symptoms, the gene ipt from Agrobacterium tumefaciens, encoding isopentenyl transferase, was transformed into Nicotiana tabacum cv. SR-1 under the control of the light-inducible rbcS-3A promoter from pea. In high light (300 mol PPFD m-2 s-1), ipt mRNA was detected and zeatin/zeatin glucoside levels were 10-fold higher than in control plants or when transformants were grown in low light (30 mol PPFD m-2 s-1). High light treatment was accompanied by increased levels of proline and osmotin when compared to low light grown transformed and untransformed control plants. Elevated in planta cytokinin levels induced responses also stimulated by salt stress, suggesting either common or overlapping signaling pathways are initiated independently by cytokinin and NaCl, setting in motion gene expression normally elicited by developmental processes such as flowering or environmental stress.Abbreviations IPT
isopentenyl, transferase
-
rbcS-3A
gene encoding a small subunit protein (SSU) of Rubisco from Pisum sativum
- Rubisco
ribulose 1,5-bisphosphate carboxylase/oxygenase 相似文献
110.
Mary Ann Kowatch Jeremiah F. Kelly Natalia A. Denisova George S. Roth 《Molecular and cellular biochemistry》1995,148(1):73-77
The age related decrease in alpha1-adrenergic stimulated inositol 1,4,5 trisphosphate (IP3) production in parotid cells of aged rats can be partially restored by treatment with S-adenosylmethionine (SAM). This effect is completely blocked by S-adenosyl homocysteine (SAH) and occurs in association with an increase in the conversion of phosphatidylethanolamine to phosphatidylcholine and a decrease in membrane viscosity. In contrast, SAM treatment actually inhibits stimulated IP3 production in cells of young rats. The membrane viscosity of these cells is lower than that of those from aged rats. Although conversion of phosphatidylethanolamine to phosphatidylcholine is enhanced, no further decrease in membrane viscosity is elicited in young cell preparations. These findings suggest that age changes in the membrane environment may result in impaired alpha1-adrenergic signal transduction and that such alterations may be at least partially reversible by SAM treatment. 相似文献