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991.
Bryony?L. Jones Tamiru?O. Raga Anke Liebert Pawel Zmarz Endashaw Bekele E.?Thomas Danielsen Anders?Krüger Olsen Neil Bradman Jesper?T. Troelsen Dallas?M. Swallow 《American journal of human genetics》2013,93(3):538-544
The persistent expression of lactase into adulthood in humans is a recent genetic adaptation that allows the consumption of milk from other mammals after weaning. In Europe, a single allele (−13910∗T, rs4988235) in an upstream region that acts as an enhancer to the expression of the lactase gene LCT is responsible for lactase persistence and appears to have been under strong directional selection in the last 5,000 years, evidenced by the widespread occurrence of this allele on an extended haplotype. In Africa and the Middle East, the situation is more complicated and at least three other alleles (−13907∗G, rs41525747; −13915∗G, rs41380347; −14010∗C, rs145946881) in the same LCT enhancer region can cause continued lactase expression. Here we examine the LCT enhancer sequence in a large lactose-tolerance-tested Ethiopian cohort of more than 350 individuals. We show that a further SNP, −14009T>G (ss 820486563), is significantly associated with lactose-digester status, and in vitro functional tests confirm that the −14009∗G allele also increases expression of an LCT promoter construct. The derived alleles in the LCT enhancer region are spread through several ethnic groups, and we report a greater genetic diversity in lactose digesters than in nondigesters. By examining flanking markers to control for the effects of mutation and demography, we further describe, from empirical evidence, the signature of a soft selective sweep. 相似文献
992.
Joachim Albers Johannes Keller Anke Baranowsky Frank Timo Beil Philip Catala-Lehnen Jochen Schulze Michael Amling Thorsten Schinke 《The Journal of cell biology》2013,200(4):537-549
Although Wnt signaling is considered a key regulatory pathway for bone formation, inactivation of β-catenin in osteoblasts does not affect their activity but rather causes increased osteoclastogenesis due to insufficient production of osteoprotegerin (Opg). By monitoring the expression pattern of all known genes encoding Wnt receptors in mouse tissues and bone cells we identified Frizzled 8 (Fzd8) as a candidate regulator of bone remodeling. Fzd8-deficient mice displayed osteopenia with normal bone formation and increased osteoclastogenesis, but this phenotype was not associated with impaired Wnt signaling or Opg production by osteoblasts. The deduced direct negative influence of canonical Wnt signaling on osteoclastogenesis was confirmed in vitro and through the generation of mice lacking β-catenin in the osteoclast lineage. Here, we observed increased bone resorption despite normal Opg production and a resistance to the anti-osteoclastogenic effect of Wnt3a. These results demonstrate that Fzd8 and β-catenin negatively regulate osteoclast differentiation independent of osteoblasts and that canonical Wnt signaling controls bone resorption by two different mechanisms. 相似文献
993.
994.
Lutz Sulimma Anke Bullach Souvik Kusari Marc Lamshöft Sebastian Zühlke Michael Spiteller 《Chirality》2013,25(6):336-340
For almost four decades, the chiral fungicides metalaxyl and furalaxyl have been in use in plant protection on a global scale. Both substances are distributed as racemic mixtures, yet the desirable interference in nucleic acid synthesis of harmful fungi only occurs by the (‐)‐R‐enantiomer. As enantioselective degradation in Scheyern (Germany) and Yaoundé (Cameroon) soils has been documented, the influence of 50 isolated microorganisms on the R/S ratio was investigated. A high‐pressure liquid chromatography method with a chiral column to separate enantiomers of metalaxyl and furalaxyl, and subsequent detection by tandem mass spectrometry, was employed. Only one of these microorganisms, a strain of Brevibacillus brevis, showed an enantioselective degradation pattern in liquid culture; the respective (‐)‐R‐enantiomers were preferably degraded. Moreover, (‐)‐R‐furalaxyl was degraded faster in cultures supplemented simultaneously with both fungicides of the same concentration. Chirality 25:336–340, 2013. © 2013 Wiley‐Liss Inc. Chirality 00:000‐000:, 2013. © 2013 Wiley Periodicals, Inc. 相似文献
995.
Lowell J. Mills Jeremy D. Wilson Anke Lange Karen Moore Barry Henwood Hazel Knipe Dominique L. Chaput Charles R. Tyler 《Journal of avian biology》2020,51(9)
Breeding ground food availability is critical to the survival and productivity of adult birds. The common cuckoo Cuculus canorus is a brood-parasitic Afro-Palearctic migrant bird exhibiting long-term (breeding) population declines in many European countries. Variation in population trend between regions and habitats suggests breeding ground drivers such as adult food supply. However, cuckoo diet has not been studied in detail since before the most significant population declines in Europe began in the mid-1980s. 20th century studies of cuckoo diet largely comprised field observations likely to carry bias towards larger prey taxa. Here we demonstrate the potential value of 1) using high-throughput DNA sequencing of invertebrate prey in faeces to determine cuckoo diet with minimal bias towards large prey taxa, and 2) using crowd-sourced digital photographs from across Britain to identify lepidopteran cuckoo prey taxa during recent years post-decline (2005–2016). DNA analysis found a high frequency of Lepidoptera, including moths of family Lasiocampidae, prominent within the past literature, but also grasshoppers (Orthoptera) and flies (Diptera) that may be overlooked by field observation methodologies. The range of larval lepidopteran prey identified from photographs largely agreed with those previously documented, with potential signs of reduced diversity, and identities of key adult prey taxa were supported by molecular results. Notably, many identified cuckoo prey taxa have shown severe declines due to agricultural intensification, suggesting this has driven spatial patterns of cuckoo loss. Landscape-scale, lowland rewilding interventions provide opportunities to understand the scale of reversal of previous agricultural intensification that may be necessary to restore prey populations sufficiently to permit recolonization by cuckoos. 相似文献
996.
Enrique Valencia Francesco de Bello Jan Lep Thomas Galland Anna E‐Vojtk Luisa Conti Jií Danihelka Jürgen Dengler David J. Eldridge Marc Estiarte Ricardo García‐Gonzlez Eric Garnier Daniel Gmez Susan Harrison Tomas Herben Ricardo Ibez Anke Jentsch Norbert Juergens Mikls Kertsz Katja Klumpp Frdrique Louault Rob H. Marrs Gbor
nodi Robin J. Pakeman Meelis Prtel Begoa Peco Josep Peuelas Marta Rueda Wolfgang Schmidt Ute Schmiedel Martin Schuetz Hana Skalova Petr milauer Marie milauerov Christian Smit Ming‐Hua Song Martin Stock James Val Vigdis Vandvik Karsten Wesche Ben A. Woodcock Truman P. Young Fei‐Hai Yu Martin Zobel Lars Gtzenberger 《植被学杂志》2020,31(5):792-802
997.
Anke F. Hofacker Andreas Voegelin Ralf Kaegi Tina Lösekann-Behrens Andreas Kappler 《Geomicrobiology journal》2013,30(2):130-139
Recent studies have reported the formation of Cu0 nanoparticles (CuNP) by suspended bacteria in pore water of periodically flooded soils, but the bacteria have not yet been identified. The aim of this study was to identify the CuNP-forming bacteria and to determine the location of CuNP formation relative to the bacterial cell surface. Electron microscopy revealed that the bacteria were rod-shaped spore formers and suggested that CuNP were formed in the periplasm. Combined results from denaturing gradient gel electrophoresis, 16S rRNA gene clone libraries, and classic microbiological cultivation techniques provided strong evidence for a Clostridium sp. strain as the CuNP-forming bacteria. Clostridia are well-adapted to frequent flooding and drying due to their ability to form spores and may play an important role in Cu cycling and metallic Cu formation in redox-dynamic environments. 相似文献
998.
Susanne Reitemeier Maria Hänse Anke Hahn Volker Schmidt Katrin Steinbach-Sobiraj Susanne Tätzner Maria-Elisabeth Krautwald-Junghanns Almuth Einspanier 《Theriogenology》2013
The success of breeding programs is limited by the sparse knowledge about endocrine regulation and biochemical reactions in the psittacine male tract. The immunocytochemical analysis of parrots' testicular tissues provides an insight into their reproductive system but is often hampered by the lack of reliable antibodies. In the present study, we tested a large panel of antibodies raised against steroid receptors, steroidogenic enzymes, relaxin peptides including their receptors, and proliferation markers on paraffin sections of testicular tissue from eight psittacine genera representing three continents. All investigated species displayed the tested markers in somatic and germ cells of testis and epididymis, even though cell distribution was partly heterogenous and in species-specific patterns. The 17β-hydroxysteroid-dehydrogenase-2, 3β-hydroxysteroid-dehydrogenase, and smooth muscle actin allowed the cross-species differentiation between active and nonactive gonads. The remaining steroidogenic enzymes, steroid receptors, relaxin peptides, and Ki67 proved to be suitable to define reproductive activity depending on the parrot species. Adapting immunocytochemical methods to different psittacines was successful, though various cellular expression patterns do not allow the transfer of results among different parrot species. However, the availability of a reliable repertory of sexual markers is important to examine reproductive biology of psittacine birds. 相似文献
999.
Hunting is often either portrayed as the ultimate means to enact a close connection between the human being and nature, or investigated in terms of its contribution to livelihoods. Through in-depth interviews and focus group discussions, we explored the meaning of hunting in the lower Omo valley, Ethiopia, and found that large game hunting was best understood as an activity that served to establish and maintain human–human relationships. Hunting was important as it created the basis for long-term bond-relations between a hunter and his friend (‘misso’) and a hunter and his honorary elder sister (‘misha’) that could be drawn on in times of hardship. By contrast, interactions between hunter and wildlife were given hardly any attention by our participants. We discuss implications in relation to the stark decline in wildlife and the degradation of grazing land over the last decades, and the consequences of our findings for conservation and development activities. 相似文献
1000.
Kerry Ann Waylen Anke Fischer Philip J. K. McGowan E. J. Milner-Gulland 《Human ecology: an interdisciplinary journal》2013,41(4):575-585
Many conservation policies advocate engagement with local people, but conservation practice has sometimes been criticised for a simplistic understanding of communities and social context. To counter this, this paper explores social structuring and its influences on conservation-related behaviours at the site of a conservation intervention near Pipar forest, within the Seti Khola valley, Nepal. Qualitative and quantitative data from questionnaires and Rapid Rural Appraisal demonstrate how links between groups directly and indirectly influence behaviours of conservation relevance (including existing and potential resource-use and proconservation activities). For low-status groups the harvesting of resources can be driven by others’ preference for wild foods, whilst perceptions of elite benefit-capture may cause reluctance to engage with future conservation interventions. The findings reiterate the need to avoid relying on simple assumptions about ‘community’ in conservation, and particularly the relevance of understanding relationships between groups, in order to understand natural resource use and implications for conservation. 相似文献