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921.
Swarmistha Devi Aribam Tomoyuki Harada Marta Elsheimer-Matulova Taketoshi Iwata Katsushi Kanehira Hirokazu Hikono Hidenori Matsui Yohsuke Ogawa Yoshihiro Shimoji Masahiro Eguchi 《PloS one》2016,11(3)
Salmonella-specific antibodies play an important role in host immunity; however, the mechanisms of Salmonella clearance by pathogen-specific antibodies remain to be completely elucidated since previous studies on antibody-mediated protection have yielded inconsistent results. These inconsistencies are at least partially attributable to the use of polyclonal antibodies against Salmonella antigens. Here, we developed a new monoclonal antibody (mAb)-449 and identified its related immunogen that protected BALB/c mice from infection with Salmonella enterica serovar Typhimurium. In addition, these data indicate that the mAb-449 immunogen is likely a major protective antigen. Using in vitro infection studies, we also analyzed the mechanism by which mAb-449 conferred host protection. Notably, macrophages infected with mAb-449-treated S. Typhimurium showed enhanced pathogen uptake compared to counterparts infected with control IgG-treated bacteria. Moreover, these macrophages produced elevated levels of pro-inflammatory cytokine TNFα and nitric oxide, indicating that mAb-449 enhanced macrophage activation. Finally, the number of intracellular bacteria in mAb-449-activated macrophages decreased considerably, while the opposite was found in IgG-treated controls. Based on these findings, we suggest that, although S. Typhimurium has the potential to survive and replicate within macrophages, host production of a specific antibody can effectively mediate macrophage activation for clearance of intracellular bacteria. 相似文献
922.
Nirmala?Devi Adivitiya Yogender?Pal?KhasaEmail author 《Applied microbiology and biotechnology》2016,100(23):9979-9994
Human interleukin-7 (hIL-7) is a therapeutically important cytokine involved in lymphocyte development and survival. In previous reports, a uniformly poor expression of hIL-7 has been shown in Escherichia coli host with the problem of inclusion body formation. In this study, the role of codon optimization and N-terminus blocking using various solubility enhancer fusion tags was explored to improve its soluble expression. The use of codon optimization strategy improved its expression to 80 ± 5 mg/L at shake flask level. The utilization of pelB leader sequence resulted in an unprocessed protein in the form of cytoplasmic inclusion bodies with lower expression yields. The N-terminus fusion of small ubiquitin-like modifier (SUMO), thioredoxin (Trx), and NusA tags increased the expression in the range of 90–140 mg/L, where >90 % of the fusion protein was obtained in soluble form. The fed-batch fermentation of SUMO-tagged hIL-7 protein was optimized at bioreactor level, where a high volumetric product concentration of 2.65 g/L was achieved by controlling the plasmid segregation instability using high antibiotic concentration. The specific product yield (YP/X) and volumetric product concentration were 1.38 and 2.55-fold higher compared to batch results, respectively. A preparative scale affinity chromatography resulted in a high recovery yield of 50.6 mg/L with ~90 % purity. The conformational property of purified recombinant hIL-7 from CD spectroscopy showed a typical helical structure with 31.5 % α-helix and 26.43 % β-sheet. The biological activity of purified protein was tested using IL-7-dependent murine immature B lymphocyte (2E8) cell line by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide salt (MTT) assay, where it showed a similar biological activity as standard control. 相似文献
923.
Y. Basavaraju B. S. Renuka Devi G. Mukhatyakka L. Purushotham Reddy G. C. Mair E. E. Roderick D. J. Penman 《Journal of biosciences》1998,23(5):585-593
A variety of marking and tagging methods were tested on common carp (Cyprinus carpio L.) with the aim of identifying suitable methods for genetic studies in this and other species of carp. Elastomer and Alcian
blue dye marking; Cold and Silver nitrate branding; Floy, Fingerling, Carlin disc and visible implant tags; and fin clipping
were all tested on a range of sizes of common carp (from mean weights of 10–25 g up to 600–800 g). The branding and tagging
methods tested did not give satisfactory retention rates. A combination of elastomer marking and fin clipping was then tested
as a method for strain identification in a growth comparison trial on catla (Catla catla Hamilton) and found to be satisfactory for this purpose. Passive integrated transponders (PIT) tags were used to individually
identify catla of wild or hatchery origin being grown for use as broodstock. These had almost 100% (98.8%) retention rates,
but are expensive compared to most other tagging methods. 相似文献
924.
Ferulic acid mediated changes in oxidative enzymes of maize seedlings: implications in growth 总被引:1,自引:0,他引:1
Maize (Zea mays L. cv. Ganga-5) seedlings were grown in the presence of ferulic acid (0.5 – 3.0 mM) for 8 d. Treatment with ferulic acid considerably decreased shoot and root length, increased the activity of peroxidase, catalase and indole-3-acetic acid (IAA) oxidase and decreased the activity of polyphenol oxidase. The increased activity of peroxidase correlated with pronounced increase in content of lignin and phenolic compounds 相似文献
925.
Eight psychrotrophic strains belonging to four different genera were screened for the presence of cold-active alkaline phosphatase in sonicated cell homogenates. An approximately 1000-fold higher activity than E. coli was detected in two psychrotrophic strains of Sphingobacterium antarcticus and one mesophilic strain of Flavobacterium multivorum. The enzymes from the psychrotrophs showed maximum activity at 37°C and were also found to be active at 0°C. Alkaline phosphatase from one psychrotrophic Sphingobacterium lost 97% of its activity when it was heated for 10 min at 62°C. This enzyme was partially purified and characterised. The production of the enzyme was repressed when the organism was grown in the presence of phosphates and its activity was inhibited on preincubation with inorganic phosphates and ethylene diamine tetracetic acid. Potassium permanganate and potassium periodate did not inhibit the activity of the enzyme. The biotechnological importance of the enzyme is discussed. 相似文献
926.
Pawan K. Dhar M. R. Kumar Satish Nayak T. Ramesh Rao Anita Joseph Sulochna Devi Usha Kuamari S. M. Bhat K. R. Bhat 《Biotechnic & histochemistry》1995,70(6):302-303
Silver staining of nucleolar organizing regions (NOR) is common, but a standard protocol is lacking. A modification of a rapid silver nitrate staining technique for NORs is presented here. Advantages of the modified technique include reliability, speed, cost and the fact that it can be carried out in the light. 相似文献
927.
Ajay Elangovan Dhivya Venkatesan Priyanka Selvaraj Md. Younus Pasha Harysh Winster Suresh Babu Mahalaxmi Iyer Arul Narayanasamy Mohana Devi Subramaniam Abilash Valsala Gopalakrishnan Nachimuthu Senthil Kumar Balachandar Vellingiri 《Journal of cellular physiology》2023,238(2):329-354
Parkinson's disease (PD) is an age associated neurological disorder which is specified by cardinal motor symptoms such as tremor, stiffness, bradykinesia, postural instability, and non-motor symptoms. Dopaminergic neurons degradation in substantia nigra region and aggregation of αSyn are the classic signs of molecular defects noticed in PD pathogenesis. The discovery of microRNAs (miRNA) predicted to have a pivotal part in various processes regarding regularizing the cellular functions. Studies on dysregulation of miRNA in PD pathogenesis has recently gained the concern where our review unravels the role of miRNA expression in PD and its necessity in clinical validation for therapeutic development in PD. Here, we discussed how miRNA associated with ageing process in PD through molecular mechanistic approach of miRNAs on sirtuins, tumor necrosis factor-alpha and interleukin-6, dopamine loss, oxidative stress and autophagic dysregulation. Further we have also conferred the expression of miRNAs affected by SNCA gene expression, neuronal differentiation and its therapeutic potential with PD. In conclusion, we suggest more rigorous studies should be conducted on understanding the mechanisms and functions of miRNA in PD which will eventually lead to discovery of novel and promising therapeutics for PD. 相似文献
928.
Inhibition of electron transport through photosystem II (PS II) by formate (HCO− 2 ) or nitrite (NO− 2 ) in the presence or absence of chloride ions was studied. The inhibition induced by HCO− 2 or NO− 2 is overcome by HCO− 3 more in the presence, than in the absence of Cl− . The data on electron transport are supported by chlorophyll a fluorescence measurements. In experiments. In experiments in which water oxidation was blocked. Cl− was found to facilitate electron transport between bound quinone A (Q− A ) and the plastoquinone (PQ) pool. It can thus be concluded that in addition to the well known site of action of Cl− on water oxidation, another site of Cl− action is between Q− A and the PQ pool. 相似文献
929.
Maria João Feio Robert M. Hughes Sónia R. Q. Serra Susan J. Nichols Ben J. Kefford Mark Lintermans Wayne Robinson Oghenekaro N. Odume Marcos Callisto Diego R. Macedo Jon S. Harding Adam G. Yates Wendy Monk Keigo Nakamura Terutaka Mori Masanao Sueyoshi Norman Mercado-Silva Kai Chen Min Jeong Baek Yeon Jae Bae Ram Devi Tachamo-Shah Deep Narayan Shah Ian Campbell Nabor Moya Francis O. Arimoro Unique N. Keke Renato T. Martins Carlos B. M. Alves Paulo S. Pompeu Subodh Sharma 《Global Change Biology》2023,29(2):355-374
Rivers suffer from multiple stressors acting simultaneously on their biota, but the consequences are poorly quantified at the global scale. We evaluated the biological condition of rivers globally, including the largest proportion of countries from the Global South published to date. We gathered macroinvertebrate- and fish-based assessments from 72,275 and 37,676 sites, respectively, from 64 study regions across six continents and 45 nations. Because assessments were based on differing methods, different systems were consolidated into a 3-class system: Good, Impaired, or Severely Impaired, following common guidelines. The proportion of sites in each class by study area was calculated and each region was assigned a Köppen-Geiger climate type, Human Footprint score (addressing landscape alterations), Human Development Index (HDI) score (addressing social welfare), % rivers with good ambient water quality, % protected freshwater key biodiversity areas; and % of forest area net change rate. We found that 50% of macroinvertebrate sites and 42% of fish sites were in Good condition, whereas 21% and 29% were Severely Impaired, respectively. The poorest biological conditions occurred in Arid and Equatorial climates and the best conditions occurred in Snow climates. Severely Impaired conditions were associated (Pearson correlation coefficient) with higher HDI scores, poorer physico-chemical water quality, and lower proportions of protected freshwater areas. Good biological conditions were associated with good water quality and increased forested areas. It is essential to implement statutory bioassessment programs in Asian, African, and South American countries, and continue them in Oceania, Europe, and North America. There is a need to invest in assessments based on fish, as there is less information globally and fish were strong indicators of degradation. Our study highlights a need to increase the extent and number of protected river catchments, preserve and restore natural forested areas in the catchments, treat wastewater discharges, and improve river connectivity. 相似文献
930.