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871.
Linda Hernández Anita Aisenberg Jorge Molina 《Ethology : formerly Zeitschrift fur Tierpsychologie》2018,124(1):1-13
In Leucauge orb‐web spiders, females form mating plugs, which play a part in cryptic female choice after they evaluate male performance during courtship and mating. Our aim was to assess sexual behavior and mating plug formation of Leucauge mariana from Colombia (CO). We carried out mating trials to describe in detail courtship and mating behavior of sexual pairs from the CO population, and then compared the results to previously collected data from a Costa Rican population (CR). In addition, we assessed the amount of sperm transferred during mating predict plug formation in CO pairs. All CO virgin females mated (n = 23), and mating plug formation occurred in only 11 cases (48%). In contrast, all CR virgin females mated (n = 43) and 74% formed mating plugs. None of the male courtship or mating behaviors that we measured in CO pairs predicted mating plug formation, in contrast to previous reports from CR. Mating plugs of CO consisted of a matrix of unknown composition (77.2%) and encapsulated and decapsulated sperm (22.8%). In CR, plug composition varied in color and consistency, but also comprised a matrix with encapsulated and decapsulated sperm. We observed female cannibalism of males in CO pairs, which had never been reported in CR pairs. Some female displays during courtship and mating were unique to each population. Different female preferences could explain the observed geographic differences between L. mariana in male traits and behaviors that could, in turn, promote reproductive isolation. More studies are needed to test this hypothesis. 相似文献
872.
Two platinum(IV) complexes, [Pt(4bt)Cl4] (4) and [Pt(dpyam)Cl4]·DMF (5) (where 4bt is 4,4′-bithiazole and dpyam is 2,2′-dipyridylamine) were prepared from the reaction of H2PtCl6·6H2O with 4,4′-bithiazole and 2,2′-dipyridylamine, respectively, in methanol. Both complexes were fully characterized and their structures were determined by the X-ray diffraction method. These complexes have a bidentate nitrogenous ligand with four chloride anions attached to a Pt(IV) metal in a distorted octahedral environment. These complexes along with three previously reported analogous complexes were used for in vitro cytotoxicity evaluation against four cultures, NIH-3T3, Caco-2, HT-29 and T47D by MTT assay. The methyl group position in the ligand plays an important role in the cytotoxicity of relevant compounds in different cultures. Interestingly, in some cases, the IC50 values of the new complexes were higher for normal cells but lower against cancer cells in comparison with cisplatin, especially in T47D (breast ductal carcinoma). 相似文献
873.
Thaqif El Khassawna Wolfgang B?cker Parameswari Govindarajan Nathalie Schliefke Britta Hürter Marian Kampschulte Gudrun Schlewitz Volker Alt Katrin Susanne Lips Miriam Faulenbach Henriette M?llmann Daniel Zahner Lutz Dürselen Anita Ignatius Natali Bauer Sabine Wenisch Alexander Claus Langheinrich Reinhard Schnettler Christian Heiss 《PloS one》2013,8(8)
Many postmenopausal women have vitamin D and calcium deficiency. Therefore, vitamin D and calcium supplementation is recommended for all patients with osteopenia and osteoporosis. We used an experimental rat model to test the hypothesis that induction of osteoporosis is more efficiently achieved in peripheral bone through combining ovariectomy with a unique multi-deficiencies diet (vitamin D depletion and deficient calcium, vitamin K and phosphorus). 14-week-old Sprague-Dawley rats served as controls to examine the initial bone status. 11 rats were bilaterally ovariectomized (OVX) and fed with multi-deficiencies diet. Three months later the treated group and the Sham group (n = 8) were euthanized. Bone biomechanical competence of the diaphyseal bone was examined on both, tibia and femur. Image analysis was performed on tibia via µCT, and on femur via histological analysis. Lower torsional stiffness indicated inferior mechanical competence of the tibia in 3 month OVX+Diet. Proximal metaphyseal region of the tibia showed a diminished bone tissue portion to total tissue in the µCT despite the increased total area as evaluated in both µCT and histology. Cortical bone showed higher porosity and smaller cross sectional thickness of the tibial diaphysis in the OVX+Diet rats. A lower ALP positive area and elevated serum level of RANKL exhibited the unbalanced cellular interaction in bone remodeling in the OVX+Diet rat after 3 month of treatment. Interestingly, more adipose tissue area in bone marrow indicated an effect of bone loss similar to that observed in osteoporotic patients. Nonetheless, the presence of osteoid and elevated serum level of PTH, BGP and Opn suggest the development of osteomalacia rather than an osteoporosis. As the treatment and fracture management of both osteoporotic and osteomalacia patients are clinically overlapping, this study provides a preclinical animal model to be utilized in local supplementation of minerals, drugs and growth factors in future fracture healing studies. 相似文献
874.
Eric L. Voorn Merel A. Brehm Anita Beelen Arnold de Haan Frans Nollet Karin H. L. Gerrits 《PloS one》2014,9(7)
Objective
To assess the reliability of contractile properties of the knee extensor muscles in 23 individuals with post-polio syndrome (PPS) and 18 age-matched healthy individuals.Methods
Contractile properties of the knee extensors were assessed from repeated electrically evoked contractions on 2 separate days, with the use of a fixed dynamometer. Reliability was determined for fatigue resistance, rate of torque development (MRTD), and early and late relaxation time (RT50 and RT25), using the intraclass correlation coefficient (ICC) and standard error of measurement (SEM, expressed as % of the mean).Results
In both groups, reliability for fatigue resistance was good, with high ICCs (>0.90) and small SEM values (PPS: 7.1%, healthy individuals: 7.0%). Reliability for contractile speed indices varied, with the best values found for RT50 (ICCs>0.82, SEM values <2.8%). We found no systematic differences between test and retest occasions, except for RT50 in healthy subjects (p = 0.016).Conclusions
In PPS and healthy individuals, the reliability of fatigue resistance, as obtained from electrically evoked contractions is high. The reliability of contractile speed is only moderate, except for RT50 in PPS, demonstrating high reliability.Significance
This was the first study to examine the reliability of electrically evoked contractile properties in individuals with PPS. Our results demonstrate its potential to study mechanisms underlying muscle fatigue in PPS and to evaluate changes in contractile properties over time in response to interventions or from natural course. 相似文献875.
Kveder M Marinić Z Krisko A Vikić-Topić D Pifat G 《Chemistry and physics of lipids》2006,141(1-2):225-229
Low density lipoprotein (LDL) particles exhibit extremely complex three-dimensional structural organization which is still not understood at the molecular level. The aim of this study was to provide the experimental evidence of a direct non-covalent interaction of the protein part with the lipid matrix. The approach was based on the combination of (1)H NMR (600 MHz) spectroscopy with thiol-specific spin labeling of the protein (apoB). It is shown that the spectral peaks assigned to the methyl head groups of phosphatidylcholine and sphingomyelin in the (1)H spectra of LDL exhibit line broadening when otherwise free thiol groups of apoB are covalently modified by methanethiosulfonate spin label. The effect is similar in the presence of water soluble paramagnetic compound. These results indicate that fragments of apoB, which are part of the receptor binding region, are directly in contact with the solvated phospholipid head groups of the lipid matrix. 相似文献
876.
Bridget Ann Knight Beverley M. Shields Adam Brook Anita Hill Dattatray S. Bhat Andrew T. Hattersley Chittaranjan S. Yajnik 《PloS one》2015,10(8)
Objective
Vitamin B12 and folate are critical micronutrients needed to support the increased metabolic demands of pregnancy. Recent studies from India have suggested that low vitamin B12 and folate concentrations in pregnancy are associated with increased obesity; however differences in diet, antenatal vitamin supplementation, and socioeconomic status may limit the generalisability of these findings. We aimed to explore the cross-sectional relationship of circulating serum vitamin B12 and folate at 28 weeks’ gestation with maternal adiposity and related biochemical markers in a white non diabetic UK obstetric cohort.Methods
Anthropometry and biochemistry data was available on 995 women recruited at 28 weeks gestation to the Exeter Family Study of Childhood Health. Associations between B12 and folate with maternal BMI and other obesity-related biochemical factors (HOMA-R, fasting glucose, triglycerides, HDL and AST) were explored using regression analysis, adjusting for potential confounders (socioeconomic status, vegetarian diet, vitamin supplementation, parity, haemodilution (haematocrit)).Results
Higher 28 week BMI was associated with lower circulating vitamin B12 (r = -0.25; P<0.001) and folate (r = -0.15; P<0.001). In multiple regression analysis higher 28 week BMI remained an independent predictor of lower circulating B12 (β (95% CI) = -0.59 (-0.74, -0.44) i.e. for every 1% increase in BMI there was a 0.6% decrease in circulating B12). Other markers of adiposity/body fat metabolism (HOMA-R, triglycerides and AST) were also independently associated with circulating B12. In a similar multiple regression AST was the only independent obesity-related marker associated with serum folate (β (95% CI) = 0.16 (0.21, 0.51))Conclusion
In conclusion, our study has replicated the previous Indian findings of associations between lower serum B12 and higher obesity and insulin resistance during pregnancy in a non-diabetic White British population. These findings may have important implications for fetal and maternal health in obese pregnancies. 相似文献877.
Honey bees display a powerful ability to recognize pollen from most plants as food and non-pollen materials as not being food. We sequentially extracted a mixed species blend of pollen with a range of non polar to polar solvents and tested the extracts for attractiveness and feeding enhancement by young bees. Both non polar and polar materials were independently attractive when added in trace quantities to a plain artificial diet. The attractants have little inherent nutritional value, as addition of phagostimulants to artificial diets did not increase the life spans of bees compared to phagostimulant-free diets. These data indicate that pollen phagostimulants consist not of a single or a few specific compounds, but rather are a suite of diverse components that additively or synergistically serve to exceed a threshold level of stimulation necessary for feeding. 相似文献
878.
In previous studies, we used a mathematical model of the thick ascending limb (TAL) to investigate nonlinearities in the tubuloglomerular
feedback (TGF) loop. That model does not represent other segments of the nephron, the water, and NaCl transport along which
may impact fluid flow rate and NaCl transport along the TAL. To investigate the extent to which those transport processes
affect TGF mediation, we have developed a mathematical model for TGF signal transduction in a short loop nephron. The model
combines a simple representation of the renal cortex with a highly-detailed representation of the outer medulla (OM). The
OM portion of the model is based on an OM urine concentrating mechanism model previously developed by Layton and Layton (Am.
J. Renal 289:F1346–F1366, 2005a). When perturbations are applied to intratubular fluid flow at the proximal straight tubule entrance, the present model predicts
oscillations in fluid flow and solute concentrations in the cortical TAL and interstitium, and in all tubules, vessels, and
interstitium in the OM. Model results suggest that TGF signal transduction by the TAL is a generator of nonlinearities: if
a sinusoidal oscillation is added to constant intratubular fluid flow, the time required for an element of tubular fluid to
traverse the TAL is oscillatory, but nonsinusoidal; those results are consistent with our previous studies. As a consequence,
oscillations in NaCl concentration in tubular fluid alongside the macula densa (MD) will be nonsinusoidal and contain harmonics
of the original sinusoidal frequency. Also, the model predicts that the oscillations in NaCl concentration at the loop-bend
fluid are smaller in amplitude than those at the MD, a result that further highlights the crucial role of TAL in the nonlinear
transduction of TGF signal from SNGFR to MD NaCl concentration. 相似文献
879.
Isolation and Functional Characterization of Two Distinct Sexual-Stage-Specific Promoters of the Human Malaria Parasite Plasmodium falciparum 总被引:4,自引:0,他引:4 下载免费PDF全文
880.
Freek Spitaels Anneleen D. Wieme Maarten Janssens Maarten Aerts Heide-Marie Daniel Anita Van Landschoot Luc De Vuyst Peter Vandamme 《PloS one》2014,9(4)
Lambic sour beers are the products of a spontaneous fermentation that lasts for one to three years before bottling. The present study determined the microbiota involved in the fermentation of lambic beers by sampling two fermentation batches during two years in the most traditional lambic brewery of Belgium, using culture-dependent and culture-independent methods. From 14 samples per fermentation, over 2000 bacterial and yeast isolates were obtained and identified. Although minor variations in the microbiota between casks and batches and a considerable species diversity were found, a characteristic microbial succession was identified. This succession started with a dominance of Enterobacteriaceae in the first month, which were replaced at 2 months by Pediococcus damnosus and Saccharomyces spp., the latter being replaced by Dekkera bruxellensis at 6 months fermentation duration. 相似文献