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41.
Malay Choudhury Takahiro Oku Shoji Yamada Masaharu Komatsu Keita Kudoh Takao Itakura Seiichi Ando 《Central European Journal of Biology》2011,6(4):545-557
Apolipoproteins such as apolipoprotein (apo) A-I, apoA-IV, and apoE are lipid binding proteins synthesized mainly in the liver
and the intestine and play an important role in the transfer of exogenous or endogenous lipids through the circulatory system.
To investigate the mechanism of lipid transport in fish, we have isolated some novel genes of the apoA-I family, apoIA-I (apoA-I isoform) 1–11, from Japanese eel by PCR amplification. Some of the isolated genes of apoIA-I corresponded to 28kDa-1 cDNAs which had already been deposited into the database and encoded an apolipoprotein with molecular
weight of 28 kDa in the LDL, whereas others seemed to be novel genes. The structural organization of all apoIA-Is consisted of four exons separated by three introns. ApoIA-I10 had a total length of 3232 bp, whereas other genes except for apoIA-I9 ranged from 1280 to 1441 bp. The sequences of apoIA-Is at the exon-intron junctions were mostly consistent with the consensus sequence (GT/AG) at exon-intron boundaries, whereas
the sequences of 3′ splice acceptor in intron 1 of apoIA-I1-7 were (AC) but not (AG). The deduced amino acid sequences of all apoIA-Is contained a putative signal peptide and a propeptide
of 17 and 5 amino acid residues, respectively. The mature proteins of apoIA-I1-3, 7, and 8 consisted of 237 amino acids, whereas
those of apoIA-I4-6 consisted of 239 amino acids. The mature apoIA-I10 sequence showed 65% identity to amino acid sequence
of apoIA-I11 which was associated with an apolipoprotein with molecular weight of 23 kDa in the VLDL. All these mature apoIA-I
sequences satisfied the common structural features depicted for the exchangeable apolipoproteins such as apoA-I, apoA-IV,
and apoE but apoIA-I11 lacked internal repeats 7, 8, and 9 when compared with other members of apoA-I family. Phylogenetic
analysis showed that these novel apoIA-Is isolated from Japanese eel were much closer to apoA-I than apoA-IV and apoE, suggesting
new members of the apoA-I family. 相似文献
42.
The effectiveness of the metal oxide nanoparticles viz. CuO and Fe2O3 as antibacterial agents against multidrug resistant biofilm forming bacteria was evaluated. CuO nanoparticles were also experimented for antibiofilm and time kill assay. The CuO displayed maximum antibacterial activity with zone of inhibition of (22 ± 1) mm against methicillin resistant Staphylococcus aureus (MRSA) followed by Escherichia coli (18 ± 1) mm. The Fe2O3 showed the zone of inhibition against MRSA of (14 ± 1) mm followed by E. coli (12 ± 1) mm. CuO proved to be more toxic than Fe2O3 nanoparticles showing significantly high antibacterial activity and found to possess dose dependent antibiofilm properties. 相似文献
43.
Résumé
Bracon greeni
Ashmead est un ectoparasite larvaire de la noctuelleEublemma amabilis
Moore, prédatrice sur laquiers en Inde. L'effet de superparasitisme sur le développement, le sex ratio et la descendance du parasite
ont été étudiés en détail.
Le pourcentage de développement du parasite décro?t quand le nombre de parasites développés par h?te augmente. La période
de développement, de l'œuf à l'éclosion de l'adulte, est de 10, 85 jours et ce délai est un peu plus court quand l'h?te est
fortement superparasité. La taille de la femelle adulte décro?t avec l'augmentation du nombre de parasites développés par
h?te.
Il y a prédominance du sexe femelle chez le parasite. Le superparasitisme ne para?t avoir que peu d'action sur le taux sexuel.
Quand plus de trois parasites se partagent un seul h?te, la longévité de l'adulte et la fécondité du parasite sont réduits.
De tels individus sont mauvais voiliers et inactifs dans leur comportement.
相似文献
44.
Molecular Evidence for the Association of Tomato leaf curl Gujarat virus with a Leaf Curl Disease of Phaseolus vulgaris L. 下载免费PDF全文
Naimuddin Kamaal Mohammad Akram Aniruddha Kumar Agnihotri 《Journal of Phytopathology》2015,163(1):58-62
A leaf curl disease with symptoms typical of begomoviruses was observed in bean (Phaseolus vulgaris) at the Main Research Farm of the Indian Institute of Pulses Research, Kanpur, India. Infected plants had severe distortion of leaves and the plants were unproductive. PCR indicated the involvement of French bean leaf curl virus (JQ866297), a recently described Begomovirus, and Tomato leaf curl Gujarat virus (ToLCGV). The full‐length genome of ToLCGV associated with leaf curl disease of bean was 2757 nucleotides long and had maximum identity (97–98%) with seven isolates of ToLCGV (AY234383, AF449999, EU573714, GQ994098, AY190290, FR819708, AF413671) and is designated as Tomato leaf curl Gujarat virus‐(IN:Knp:Bean:2013) (KF440686). To the best of our knowledge, this is the first record of ToLCGV infecting a leguminous host, P. vulgaris. 相似文献
45.
Microsatellites are the markers of choice due to their high abundance reproducibility, degree of polymorphism and co-dominant nature. These are mainly used for studying the genetic variability in different species and Marker assisted selection. Expressed Sequence Tags (ESTs) serve as the main resource for Simple Sequence Repeats (SSRs). The computational approach for detecting SSRs and developing SSR markers from EST-SSRs is preferred over the conventional methods as it reduces time and cost to a great extent. The available EST sequence databases, various web interfaces and standalone tools provide the platform for an easy analysis of the EST sequences leading to the development of potential EST-SSR Markers. This paper is an overview of in silico approach to develop SSR Markers from the EST sequence using some of the most efficient tools that are available freely for academic purpose. 相似文献
46.
Murai H Qi H Choudhury B Wild J Dharajiya N Vaidya S Kalita A Bacsi A Corry D Kurosky A Brasier A Boldogh I Sur S 《PloS one》2012,7(2):e30280
Background
A series of epidemiologic studies have identified the fungus Alternaria as a major risk factor for asthma. The airway epithelium plays a critical role in the pathogenesis of allergic asthma. These reports suggest that activated airway epithelial cells can produce cytokines such as IL-25, TSLP and IL-33 that induce Th2 phenotype. However the epithelium-derived products that mediate the pro-asthma effects of Alternaria are not well characterized. We hypothesized that exposure of the airway epithelium to Alternaria releasing cytokines that can induce Th2 differentiation.Methodology/Principal Finding
We used ELISA to measure human and mouse cytokines. Alternaria extract (ALT-E) induced rapid release of IL-18, but not IL-4, IL-9, IL-13, IL-25, IL-33, or TSLP from cultured normal human bronchial epithelial cells; and in the BAL fluids of naïve mice after challenge with ALT-E. Both microscopic and FACS indicated that this release was associated with necrosis of epithelial cells. ALT-E induced much greater IL-18 release compared to 19 major outdoor allergens. Culture of naïve CD4 cells with rmIL-18 induced Th2 differentiation in the absence of IL-4 and STAT6, and this effect was abrogated by disrupting NF- κB p50 or with a NEMO binding peptide inhibitor.Conclusion/Significance
Rapid and specific release of IL-18 from Alternaria-exposed damaged airway epithelial cells can directly initiate Th2 differentiation of naïve CD4+ T-cells via a unique NF-κB dependent pathway. 相似文献47.
Rahman Anisur Choudhury Parthankar Talukdar Nazimur Rahman 《Biological trace element research》2019,188(2):451-460
Biological Trace Element Research - This study investigated trace elements in the different organs of Eonycteris spelaea, a hill cave from the Bhuban Hills of Sonai Reserve Forest, Cachar, Assam... 相似文献
48.
Auriculostoma n. gen. (Trematoda: Allocreadiidae) is proposed for Auriculostoma astyanace n. sp. from the intestine of the characid fish Astyanax fasciatus in the Atlantic coastal drainages of Nicaragua. The new genus differs from all papillose allocreadiid genera, except Bunoderella, in possessing 2 pairs of muscular oral papillae (instead of 1 or 3), of which the ventrolateral pair is moderately developed and the dorsolateral papillae are long and auricular. Auriculostoma differs from Bunoderella Schell, 1964, in having lateral vitelline follicles, completely separated or confluent only in the posttesticular region, a uterus limited to the pretesticular region or with a few eggs at the level of the testes, and a long cirrus sac that overlaps the acetabulum or usually reaches posteriorly to the ovarian level. Three other allocreadiid species, all from South American freshwater fishes and each of which had previously been placed in Crepidostomum, are transferred to Auriculostoma based on the presence of the diagnostic muscular oral papillae. These include Crepidostomum platense Szidat, 1954, C. macrorchis Szidat, 1954, and C. stenopteri Ma?é-Garzón and Gascón, 1973. Diagnostic features for Auriculostoma also include mainly pretesticular uterus, lateral vitellaria with variation in posttesticular confluence, and tandem testes. The genus appears to be typically associated with neotropical siluriforms (catfishes) and characiforms (tetras). 相似文献
49.
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