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181.
The mitochondrial membrane permeability transition induced byCa2+ is inhibited by quinine in a dose-dependent fashion.Competition experiments strongly suggest that quinine displacesCa2+ bound to the inner membrane. This is supported byexperiments showing that quinine inhibits Ca2+-dependent butnot Ca2+-independent mitochondrial swelling induced byphenylarsine oxide. As with Ca2+ chelators, quinine inducespermeability transition pore closure preventing the contraction induced bypoly(ethylene glycol) 2000 in mitochondria preswollen by incubation in KSCNmedium containing Ca2+ and inorganic phosphate. These resultssuggest that quinine dislodges Ca2+ bound to the protein site,which triggers pore opening. 相似文献
182.
Laura I. Ferreyra Juan C. Vilardi Anibal Verga Victoria López Beatriz O. Saidman 《Plant Systematics and Evolution》2013,299(6):1157-1173
The section Algarobia of genus Prosopis includes promising species for reforestation and afforestation programmes in arid and semiarid regions, mainly of the Americas. Many interspecific natural hybrid combinations have been described in this group. In this paper we analysed a hybrid zone in Chaco biogeographical province in Argentina, where P. ruscifolia and P. alba overlap and hybridise producing intermediate fertile hybrid forms. Eleven morphological traits and 76 loci RAPD were analysed to determine the effect of hybridization between these species. The comparison of morphological traits among groups yielded significant or highly significant differences for all traits. Estimates of H e in P. alba and P. ruscifolia did not differ from each other, but both showed significantly lower values than the hybrid group. The analysis of correlations between shared phenotypes and pair-wise relationships estimated from RAPD gave also strong support to the hypothesis that most of the phenotypic traits analysed have significant heritability. The analyses of population structure and clustering based on morphological and molecular data by DAPC and STRUCTURE were rather consistent and indicated that the three morphotypes studied here are differentiated with low overlapping. All results indicated that despite the occurrence of natural hybridization and introgression, interspecific gene flow would be limited by hybrid breakdown or natural selection favouring the maintenance of species integrity. 相似文献
183.
Deiviane Aparecida Calegar Beatriz Coronato Nunes Kerla Joeline Lima Monteiro Jéssica Pereira dos Santos Helena Keiko Toma Tais Ferreira Gomes Marli Maria Lima Márcio Neves Bóia Filipe Anibal Carvalho-Costa 《Memórias do Instituto Oswaldo Cruz》2016,111(2):114-119
This study aimed to estimate the frequency, associated factors, and molecular
characterisation of Entamoeba histolytica, Entamoeba
dispar, Entamoeba moshkovskii, andEntamoeba
hartmanni infections. We performed a survey (n = 213 subjects) to obtain
parasitological, sanitation, and sociodemographic data. Faecal samples were processed
through flotation and centrifugation methods.E. histolytica,
E. dispar, E. moshkovskii, and E.
hartmanni were identified by nested-polymerase chain reaction (PCR). The
overall prevalence of infection was 22/213 (10.3%). The infection rate among subjects
who drink rainwater collected from roofs in tanks was higher than the rate in
subjects who drink desalinated water pumped from wells; similarly, the infection rate
among subjects who practice open defecation was significantly higher than that of
subjects with latrines. Out of the 22 samples positive for morphologically
indistinguishableEntamoeba species, the differentiation by PCR
was successful for 21. The species distribution was as follows: 57.1% to E.
dispar, 23.8% to E. histolytica, 14.3% toE.
histolytica and E. dispar, and 4.8% E.
dispar and E. hartmanni. These data suggest a high
prevalence of asymptomatic infection by the group of morphologically
indistinguishable Entamoeba
histolytica/dispar/moshkovskiicomplex
and E. hartmanni species. In this context of water scarcity, the
sanitary and socioenvironmental characteristics of the region appear to favour
transmission. 相似文献
184.
185.
Mauricio Grecco Zaia Sandra Regina Pereira de Oliveira Cynthia Aparecida de Castro Edson Garcia Soares Ana Afonso Luis Gustavo S Monnazzi Oscar Peitl Filho Lúcia Helena Faccioli Fernanda de Freitas Anibal 《Memórias do Instituto Oswaldo Cruz》2015,110(6):726-731
The protective effect of infectious agents against allergic reactions has been
thoroughly investigated. Current studies have demonstrated the ability of some
helminths to modulate the immune response of infected hosts. The objective of the
present study was to investigate the relationship between Toxocara
canis infection and the development of an allergic response in mice
immunised with ovalbumin (OVA). We determined the total and differential blood and
bronchoalveolar lavage fluid cells using BALB/c mice as a model. To this end, the
levels of interleukin (IL)-4, IL-5 and IL-10 and anti-OVA-IgE were measured using an
ELISA. The inflammatory process in the lungs was observed using histology slides
stained with haematoxylin and eosin. The results showed an increase in the total
number of leukocytes and eosinophils in the blood of infected and immunised animals
at 18 days after infection. We observed a slight lymphocytic inflammatory infiltrate
in the portal space in all infected mice. Anti-OVA-IgE levels were detected in
smaller proportions in the plasma of immunised and infected mice compared with mice
that were only infected. Therefore, we concluded that T. canis
potentiates inflammation in the lungs in response to OVA, although anti-OVA-IgE
levels suggest a potential reduction of the inflammatory process through this
mechanism. 相似文献