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81.
Proteomics and transcriptomics investigation on longissimus muscles in Large White and Casertana pig breeds 总被引:1,自引:0,他引:1
Murgiano L D'Alessandro A Egidi MG Crisà A Prosperini G Timperio AM Valentini A Zolla L 《Journal of proteome research》2010,9(12):6450-6466
Consumer complaints against the blandness of modern lean meat and the frequent reference to the more strongly flavored meat that was available years ago have prompted reconsideration of high fat-depositing typical pig breeds. Casertana and Large White pig breeds are characterized by a different tendency toward fat accumulation as they exhibit opposite genetic and physiological traits with respect to the energy metabolism. These physiological differences were investigated in longissimus lumborum muscles through proteomics (2-DE, MS/MS) and microarray approaches. Data were analyzed for pathway and network analyses, as well as GO term enrichment of biological functions. As a result, Casertana showed a greater amount of proteins involved in glycolitic metabolism and mainly rely on fast-mobilizable energy sources. Large White overexpressed cell cycle and skeletal muscle growth related genes. Metabolic behavior and other implications are discussed. 相似文献
82.
Carru C Zinellu A Galistu F Barca M Pasciu V Lumbau F Sanna B Tadolini B Deiana L 《Journal of biochemical and biophysical methods》2004,61(3):271-281
LDL-oxidation is considered a contributing factor to the development of atherosclerotic lesions. However, to utilise the oxidative state of LDL as a marker of cardiovascular risk, reliable analytical methods for its detection must be defined. We have compared three methods for their capacity to evaluate the difference in the oxidation state of isolated LDL subjected to either dialysis (D-LDL) or gel filtration (F-LDL) to remove EDTA. Their susceptibility to oxidation promoted by Cu(2+) was monitored by following the time course of conjugated diene (CD) and lipid hydroperoxide (ROOH) accumulation. The relative electrophoretic mobility (REM) of the same LDL samples was evaluated by capillary electrophoresis. As measured by all three methods, F-LDL are less prone to oxidation than D-LDL when added with CuSO(4). REM of F-LDL and D-LDL significantly differs already before the addition of the metal catalyst, whereas CD and ROOH contents become significantly different only after it. Besides confirming that a rapid centrifugation followed by gel filtration is a more convenient procedure than dialysis to remove EDTA during LDL isolation, our study suggests the REM of isolated-LDL as the biochemical marker of choice in the evaluation of its oxidative state. 相似文献
83.
Jay S. Berger James A. Ernst Angelo C. Nicoletta Leslie A. Hull Jianxin Yang Rong Qiu 《Journal of biomolecular structure & dynamics》2013,31(3):285-291
Abstract Whether or not surface salt bridges have a strong stabilizing effect on the native structure in proteins remains uncertain. Previous studies of model peptides have shown that salt bridges spaced at i,i+4 along the chain are more stabilizing than those spaced at i,i+3, with a preference for the order acid-base rather than base-acid from N to C terminus. An analysis of the effect of spacing the ion pairs in short helical peptides is presented, in which acidic and basic side chains spaced two or three residues apart alternate along the chain. The mixed spacing proves to be stabilizing relative to pure spacings. A control peptide in which salt bridges were spaced uniformly three residues apart proved to form a β-sheet structure rather than a-helix. This is due to formation of a silk-like apolar face consisting of alanine side chains; the mesoscopic structure formed by these sheets can be imaged by scanning microscopy. 相似文献
84.
Abstract The discover and morphological characters of a Dematiaceous Hyphomycetes are described. The material, studied under a morphological point of view directly collected on the natural substratum, allows us to establish the appartenance to the genus Chaetopsina. Since this species does not appeare described till now, the Authors propose for it the nomenclature ivoriensis as a new species. 相似文献
85.
Rachael A. Murtaugh Angelo P. Capparella John C. Kostelnick Greg D. Johnson 《The Journal of wildlife management》2019,83(6):1345-1351
Bat fatality at wind energy facilities is a conservation issue, but its effect on bat populations is difficult to estimate. We have little understanding of wind turbine effects on bat population persistence, in part because we have poor knowledge of bat migration pathways and hence the source populations for individual fatalities. We used deuterium ratio analysis combined with genetic algorithm for rule-set prediction and the web-based isoscapes modeling, analysis, and prediction in a geographic information system environment as a novel approach. Our objectives were to explore the utility of these methods together and map the geographic extents of eastern red bat (Lasiurus borealis) specimens salvaged in 2008–2010 from a single, 92-km2 wind energy facility in Illinois, USA. Results indicate that combining these methods can be successful and support their use with species where ranges may be less well defined. Because of the migratory nature of this species and the range of deuterium values of pixels in our isotope model, we predicted that 18% and 82% of the specimens would have isotope results inside and outside of the wind facility's isocline respectively. We concluded that 71.4% of the specimens had isotope signatures placing them outside the wind facility's isocline. It could be argued that the wide distribution of bat fatalities dilutes the overall effect of those fatalities on the bat species; however, if other facilities show a similar pattern, each facility could have cumulative and far reaching population-level effects. © 2019 The Wildlife Society. 相似文献
86.
Yumnam Silla Swati Varshney Arjun Ray Trayambak Basak Angelo Zinellu Varatharajan Sabareesh Ciriaco Carru Shantanu Sengupta 《Proteins》2019,87(8):625-634
An increased level of homocysteine, a reactive thiol amino acid, is associated with several complex disorders and is an independent risk factor for cardiovascular disease. A majority (>80%) of circulating homocysteine is protein bound. Homocysteine exclusively binds to protein cysteine residues via thiol disulfide exchange reaction, the mechanism of which has been reported. In contrast, homocysteine thiolactone, the cyclic thioester of homocysteine, is believed to exclusively bind to the primary amine group of lysine residue leading to N-homocysteinylation of proteins and hence studies on binding of homocysteine thiolactone to proteins thus far have only focused on N-homocysteinylation. Although it is known that homocysteine thiolactone can hydrolyze to homocysteine at physiological pH, surprisingly the extent of S-homocysteinylation during the exposure of homocysteine thiolactone with proteins has never been looked into. In this study, we clearly show that the hydrolysis of homocysteine thiolactone is pH dependent, and at physiological pH, 1 mM homocysteine thiolactone is hydrolysed to ~0.71 mM homocysteine within 24 h. Using albumin, we also show that incubation of HTL with albumin leads to a greater proportion of S-homocysteinylation (0.41 mol/mol of albumin) than N-homocysteinylation (0.14 mol/mol of albumin). S-homocysteinylation at Cys34 of HSA on treatment with homocysteine thiolactone was confirmed using LC-MS. Further, contrary to earlier reports, our results indicate that there is no cross talk between the cysteine attached to Cys34 of albumin and homocysteine attached to lysine residues. 相似文献
87.
88.
Sérgio Oyama Jr. M. Terêsa M. Miranda Sumika Kiyota Angelo G. Gambarini 《Journal of molecular modeling》1999,5(4):90-96
Molecular modeling has allowed us to propose that one main contact surface of the Fibroblast Growth Factor Receptor -1 (FGFR-1) to the ligand FGF-1 is formed by a 16 amino acid sequence comprised by the C-terminal region of the domain II (DII) plus the hinge linking DII and DIII domains and the N-terminal region of domain III (DIII). Therefore, this sequence was used to design the following three peptides: Ac-YQLDVVERS-NH2 (R1); Ac-YQLDVVERSPHRPILQ-NH2 (R2) and Ac-RSPHRPILQ-NH2 (R3). The synthetic peptides were tested in their ability to inhibit the mitogenic activity of FGF-1 and FGF-2 in cultured Balb/c 3T3 fibroblasts. The results showed that R1 and R2 inhibited the activity of FGF-1 (ID50 = 40 -50 7M) but not that of FGF-2. Molecular modeling studies of R1 and its docking to FGF-1 suggested that this peptide could assume a conformation very similar to that found in the corresponding segment of FGFR-1. All these results support our hypothesis that the C-terminal residues of the DII domain, represented by peptide R1, are part of a surface responsible for the binding of FGF-1 to FGFR-1 but not of FGF-2. Also, they indicate that peptide R1 may be useful for the development of small selective peptide inhibitors of the FGF-1 biological activities. 相似文献
89.
In higher plant cells, there are some enzymes capable of utilizing pyrophosphate (PPi ) as an energy donor. Among these, membrane-bound proton pumping pyrophosphatases (H+ -PPi ase) have been identified. In addition to the well-known vacuolar H+ -PPi ase (V-PPi ase), there is evidence for the presence of a mitochondrial H+ -PPi ase. This enzyme is localized on the inner surface of the inner membrane and catalyzes the specific hydrolysis of PPi , coupled to proton transport, with a H+ /PPi stoichiometry of ca 2. This activity is Mg2+ -requiring, is stimulated by monovalent cations, and is inhibited by Ca2+ , F− and diphosphonates. The H+ -PPi ase contains a catalytic head which is constituted by a 35-kDa protein which is loosely bound to the inner membrane. This protein exhibits a PPi ase activity, stimulated by phospholipids, with characteristics very similar to the membrane-bound enzyme. The mitochondrial PPi ase is distinct from the V-PPi ase, because an antibody raised against the 35-kDa protein does not react with tonoplast membranes. The mitochondrial H+ -PPi ase seems to have an F-type structure, similar to the F-ATP synthase and the membrane-bound PPi ases from mammalian and yeast mitochondria. It is suggested that, beside synthesizing PPi , this enzyme may act as a buffer for the electrochemical proton gradient, by hydrolyzing PPi, during conditions of oxygen deprivation. 相似文献
90.
Pavan S Schiavulli A Appiano M Marcotrigiano AR Cillo F Visser RG Bai Y Lotti C Ricciardi L 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,123(8):1425-1431
The powdery mildew disease affects several crop species and is also one of the major threats for pea (Pisum sativum L.) cultivation all over the world. The recessive gene er1, first described over 60 years ago, is well known in pea breeding, as it still maintains its efficiency as a powdery mildew
resistance source. Genetic and phytopathological features of er1 resistance are similar to those of barley, Arabidopsis, and tomato mlo powdery mildew resistance, which is caused by the loss of function of specific members of the MLO gene family. Here, we describe the obtainment of a novel er1 resistant line by experimental mutagenesis with the alkylating agent diethyl sulfate. This line was found to carry a single
nucleotide polymorphism in the PsMLO1 gene sequence, predicted to result in premature termination of translation and a non-functional protein. A cleaved amplified
polymorphic sequence (CAPS) marker was developed on the mutation site and shown to be fully co-segregating with resistance
in F2 individuals. Sequencing of PsMLO1 from three powdery mildew resistant cultivars also revealed the presence of loss-of-function mutations. Taken together, results
reported in this study strongly indicate the identity between er1 and mlo resistances and are expected to be of great breeding importance for the development of resistant cultivars via marker-assisted
selection. 相似文献