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41.
Angela Haczku 《Respiratory research》2012,13(1):80
The pulmonary innate immune system is heavily implicated in the perpetual airway inflammation and impaired host defense characterizing Chronic Obstructive Pulmonary Disease (COPD). The airways of patients suffering from COPD are infiltrated by various immune and inflammatory cells including macrophages, neutrophils, T lymphocytes, and dendritic cells. While the role of macrophages, neutrophils and T lymphocytes is well characterized, the contribution of dendritic cells to COPD pathogenesis is still the subject of emerging research. A paper by Botelho and colleagues in the current issue of Respiratory Research investigates the importance of dendritic cell recruitment in cigarette-smoke induced acute and chronic inflammation in mice. Dendritic cells of the healthy lung parenchyma and airways perform an important sentinel function and regulate immune homeostasis. During inflammatory responses the function and migration pattern of these cells is dramatically altered but the underlying mechanisms are incompletely understood. Botelho and colleagues demonstrate here the importance of IL-1R1/IL-1α related mechanisms including CCL20 production in cigarette-smoke induced recruitment of dendritic cells and T cell activation in the mouse lung. 相似文献
42.
Costa DL Dias-Melicio LA Acorci MJ Bordon AP Tavian EG Peraçoli MT Soares AM 《Microbiology and immunology》2007,51(1):73-80
Paracoccidioidomycosis, a deep mycosis endemic in Latin America, is a chronic granulomatous disease caused by the fungus Paracoccidioides brasiliensis. Phagocytic cells play a critical role against this fungus, and several studies have shown the effects of activator and suppressive cytokines on macrophage and monocyte functions. However, studies on polymorphonuclear neutrophils (PMNs), that are the first cells recruited to the infection sites, are scarcer. Thus, the objective of this paper was to assess whether interleukin-10 (IL-10), a potent anti-inflammatory cytokine, is able to block the activity of IFN-gamma-activated human PMNs upon P. brasiliensis intracellular killing, in vitro. The results showed that IFN-gamma-activated PMNs have an effective fungicidal activity against the fungus. This activity was associated with the release of high levels of H(2)O(2), the metabolite involved in phagocytic cells antifungal activities. However, the concomitant incubation of these cells with IFN-gamma and IL-10 significantly blocked IFN-gamma activation. As a consequence, PMNs killing activity and H(2)O(2) release were inhibited. Together, our results show the importance of PMNs exposure to activator or suppressor cytokines in the early stages of paracoccidioidomycosis infection. 相似文献
43.
Elizabeth?R?Unger Rosane?Nisenbaum Harvey?Moldofsky Angela?Cesta Christopher?Sammut Michele?Reyes William?C?ReevesEmail author 《BMC neurology》2004,4(1):6
Background
Chronic fatigue syndrome (CFS) is a disabling condition that affects approximately 800,000 adult Americans. The pathophysiology remains unknown and there are no diagnostic markers or characteristic physical signs or laboratory abnormalities. Most CFS patients complain of unrefreshing sleep and many of the postulated etiologies of CFS affect sleep. Conversely, many sleep disorders present similarly to CFS. Few studies characterizing sleep in unselected CFS subjects have been published and none have been performed in cases identified from population-based studies.Methods
The study included 339 subjects (mean age 45.8 years, 77% female, 94.1% white) identified through telephone screen in a previously described population-based study of CFS in Wichita, Kansas. They completed questionnaires to assess fatigue and wellness and 2 self-administered sleep questionnaires. Scores for five of the six sleep factors (insomnia/hypersomnia, non-restorative sleep, excessive daytime somnolence, sleep apnea, and restlessness) in the Centre for Sleep and Chronobiology's Sleep Assessment Questionnaire© (SAQ©) were dichotomized based on threshold. The Epworth Sleepiness Scale score was used as a continuous variable.Results
81.4% of subjects had an abnormality in at least one SAQ© sleep factor. Subjects with sleep factor abnormalities had significantly lower wellness scores but statistically unchanged fatigue severity scores compared to those without SAQ© abnormality. CFS subjects had significantly increased risk of abnormal scores in the non-restorative (adjusted odds ratio [OR] = 28.1; 95% confidence interval [CI]= 7.4–107.0) and restlessness (OR = 16.0; 95% CI = 4.2–61.6) SAQ© factors compared to non-fatigued, but not for factors of sleep apnea or excessive daytime somnolence. This is consistent with studies finding that, while fatigued, CFS subjects are not sleepy. A strong correlation (0.78) of Epworth score was found only for the excessive daytime somnolence factor.Conclusions
SAQ© factors describe sleep abnormalities associated with CFS and provide more information than the Epworth score. Validation of these promising results will require formal polysomnographic sleep studies.44.
Esposito F Kharlamova T Distinto S Zinzula L Cheng YC Dutschman G Floris G Markt P Corona A Tramontano E 《The FEBS journal》2011,278(9):1444-1457
45.
Reyer Heinz-Ulrich; Bollmann Kurt; Schlapfer Alex R.; Schymainda Angela; Klecack Gabriela 《Behavioral ecology》1997,8(5):534-543
Behavioral ecology has successfully explained the diversityin social mating systems through differences in environmentalconditions, but diversity in genetic mating systems is poorlyunderstood. The difference is important in situations whereparents care for extrapair young (EPY) originating from extrapairpaternity (EPP), extrapair maternity (EPM), and intraspecificbrood parasitism (IBP). In birds, IBP and EPM are rare, butEPP is widespread and highly variable among species and populations.Explanations for this variability are controversial, mainlybecause detailed ecological information is usually lacking inpaternity studies. Here we present results of the first studyto identify the ecological determinants of extrapair activitiesfor both sexes of the same species, the water pipit (Anthusspinoletta). DNA fingerprints of 1052 young from 258 nests revealedEPP in 5.2% of the young from 12.4% of the nests. EPM and IBP,both involving egg dumping (EDP), each occurred in 0.5% of theyoung from 1.9% of the nests. Nests with and without EPY couldnot be distinguished by traits of the breeders and by reproductivesucccess, but they differed with respect to ecology: nests withEPP young were characterized by asynchronous clutch initiation,nests with EPM and IBP young were characterized by higher overlapwith neighboring territories and closer proximity to communalfeeding sites. We suggest that chance events, resulting fromthe temporal and spatial distribution of broods, offer a betterexplanation for the occurence of extrapair activities than femalesearch for genetic or phenotypic benefits. This possibilityof "accidental" extrapair reproduction as an "ecological epiphenomenon"with low potential for selection should also be considered forspecies other than the water pipit. 相似文献
46.
47.
Dos Santos RA Batista J Rosa SI Torquato HF Bassi CL Ribeiro TA De Sousa PT Bessera AM Fontes CJ Da Silva LE Piuvezam MR 《Parasitology》2011,138(10):1224-1233
Leishmaniasis is one of the neglected diseases. High cost, systemic toxicity, and diminished efficacy due to development of resistance by the parasites has a negative impact on the current treatment options. Thus, the search for a new, effective and safer anti-leishmanial drug becomes of paramount importance. Compounds derived from natural products may be a better and cheaper source in this regard. This study evaluated the in vitro anti-leishmanial activity of Spiranthera odoratíssima (Rutaceae) fractions and isolated compounds, using promastigote and amastigote forms of different Leishmania species. J774 A.1 macrophage was used as the parasite host cell for the in vitro assays. Evaluations of cytoxicity, nitric oxide (NO), interleukin-10 and in silico analysis were carried out. In vitro experiments showed that the fruit hexanic fraction (Fhf) and its alkaloid skimmianine (Skm) have a significant (P<0·001) effect against L. braziliensis. This anti-L. braziliensis activity of Fhf and Skm was due to increased production of NO and attenuation of IL-10 production in the macrophages at concentrations ranging from 1·6 to 40·0 μg/ml. The in silico assay demonstrated significant interaction between Skm and amino acid residues of NOS2. Skm is thus a promising drug candidate for L. braziliensis due to its potent immunomodulatory activity. 相似文献
48.
Manoj Kumar Vladimir León Angela De Sisto Materano Olaf A. Ilzins 《World journal of microbiology & biotechnology》2007,23(2):211-220
A Bacillus sp. strain DHT, isolated from oil-contaminated soil, grew and produced biosurfactant when cultured in variety of substrate
at salinities of up to 100 g l−1 and temperatures up to 45°C. It was capable of utilizing crude oil, fuels, various pure alkanes and PAHs as a sole carbon
and energy source across a wide range of temperature and salinity. Over the range evaluated, the degradation of hydrocarbon
and biosurfactant production was not influenced by salinity (0–10% wv−1) and temperature (30–45°C). The biosurfactant produced by the organism emulsified a range of hydrocarbons with hexadecane
as the best substrate and toluene as the poorest. From 16S rDNA analysis, strain DHT was related to Bacillus licheniformis. 相似文献
49.
Abscisic acid is a hormonal stress signal that moves in the xylem from the root to the different parts of the shoot where it regulates transpirational water loss and leaf growth. The factors that modify the intensity of the ABA signal in the xylem are of particular interest because target cells recognize concentrations. ABA(xyl), will be decreased as radial water flow through the roots is increased, assuming that radial ABA transport occurs in the symplast only. Such dilutions of the plant hormone concentration can be compensated in different ways, which help to keep the ABA-concentrations in the xylem constant: (i) apoplastic bypass flows of ABA, (ii) ABA flows between the stem parenchyma and the xylem during transport and (iii) the action of beta-D-glucosidases that release free ABA from its conjugates to the root cortex and the leaf apoplast. The significance of reflection coefficients (sigma(ABA)), permeability coefficients of membranes (P(S)(ABA)) and apoplastic barriers for ABA is discussed. 相似文献