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51.
Six analogues of geranyl pyrophosphate (the monophosphates of geraniol and tetrahydrogeraniol, and the pyrophosphates of nerol, octan-1-ol, tetrahydrogeraniol and citronellol) were synthesized, and were found to be inhibitors of pig liver prenyl- (geranyl-)transferase. The effects of each analogue were analysed in kinetic experiments, which showed the pyrophosphates of citronellol, tetrahydrogeraniol and octan-1-ol to be the most potent inhibitors. The results are interpreted to support a previous hypothesis that the main forces in the binding of substrates to prenyltransferase are non-specific lipophilic forces and a pyrophosphate-binding force. 相似文献
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An Improved Diluent for Rubella Hemagglutination and Hemagglutination-Inhibition Tests 总被引:3,自引:2,他引:1 下载免费PDF全文
Angela E. Auletta Gary L. Gitnick Carrie E. Whitmire John L. Sever 《Applied microbiology》1968,16(5):691-694
Rubella hemagglutinating (HA) antigen was prepared in BHK-21 tissue as 5% cell suspensions and from unconcentrated and 20× concentrated infected supernatant fluids. In some instances, unconcentrated fluids were treated with Tween 80 and ether; cell suspensions were treated with ether alone. Preparations were tested for HA activity in dextrose-gelatin-Veronal (DGV) buffer solutions; 0.85% NaCl; Sorenson's phosphate-buffered saline, pH 7.2; and a diluent of 0.9% NaCl, 0.1% CaCl2 (anhydrous), and 0.1% MgSO4·7H2O. HA titers were consistently two- to fourfold higher in the saline with added Ca++ and Mg++ than in DGV. Hemagglutination-inhibition titers of paired human sera were the same in either diluent. It is suggested that the interaction between rubella HA antigen and the red cells of young (less than 1-day-old) chicks may be at least partially ion dependent and that titers are enhanced by increased quantities of divalent cations. 相似文献
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The levels of airborne contamination in various areas of two hospitals were determined during a 15-month sampling period, using Casella and Andersen volumetric samplers. Based on nearly 5,000 samples, the mean count per ft3 ranged from 4.5 in obstetric-gynecology delivery rooms to 72.4 in waste-handling areas. The mean count for the entire hospital environment was on the order of 20 contaminants per ft3; 48% were associated with particles >5 μ diam, 30% with particles between 2 and 6 μ diam, and 22% with particles <2 μ diam. The airborne contamination was influenced by traffic, activity, ventilation considerations, and gross surface contamination, but not markedly by seasonal changes. When suitable control measures were implemented, the level of contamination could be diminished and kept low. 相似文献
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Over 10,000 airborne microorganisms, isolated from various areas of two hospitals, were characterized according to colonial and microscopic morphology and certain physiological reactions, including penicillin resistance and hemolysis. On the basis of all isolates examined during a 15-month period, 42.6% were gram-positive cocci, 19.2% were gram-positive rods, 14.0% were gram-negative rods, 17.1% were molds, 2.2% were actinomycetes, 1.2% were yeasts, and the remainder were assorted diphtheroids and coccobacillary types. The distribution of types varied according to hospital area, locations within a given area, and level of gross airborne contamination, but did not vary significantly with season of the year. There appeared to be some relationship between contaminant particle size and type of organism associated with the particle. Distribution of penicillin-resistant types differed markedly in different hospital areas, with proportions ranging from 21.4% in surgery areas to 4.3% in incinerator rooms. Of all gram-positive cocci isolated, 34.9% were hemolytic, and 16.4% were penicillin-resistant. 相似文献
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