排序方式: 共有26条查询结果,搜索用时 15 毫秒
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Aikaterini Zgouridou Eirini Tripidaki Ioannis A. Giantsis John A. Theodorou Maria Kalaitzidou Dionysios E. Raitsos Athanasios Lattos Apostolia-Maria Mavropoulou Sarantis Sofianos Dimitrios Karagiannis Ilias Chaligiannis Andreas Anestis Nikos Papadakis Konstantinos Feidantsis Dionysia Mintza Alexandra Staikou Basile Michaelidis 《Environmental microbiology》2022,24(3):1012-1034
Global warming affects the aquatic ecosystems, accelerating pathogenic microorganisms' and toxic microalgae's growth and spread in marine habitats, and in bivalve molluscs. New parasite invasions are directly linked to oceanic warming. Consumption of pathogen-infected molluscs impacts human health at different rates, depending, inter alia, on the bacteria taxa. It is therefore necessary to monitor microbiological and chemical contamination of food. Many global cases of poisoning from bivalve consumption can be traced back to Mediterranean regions. This article aims to examine the marine bivalve's infestation rate within the scope of climate change, as well as to evaluate the risk posed by climate change to bivalve welfare and public health. Biological and climatic data literature review was performed from international scientific sources, Greek authorities and State organizations. Focusing on Greek aquaculture and bivalve fisheries, high-risk index pathogenic parasites and microalgae were observed during summer months, particularly in Thermaikos Gulf. Considering the climate models that predict further temperature increases, it seems that marine organisms will be subjected in the long term to higher temperatures. Due to the positive linkage between temperature and microbial load, the marine areas most affected by this phenomenon are characterized as ‘high risk’ for consumer health. 相似文献
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Tsakiridis A Tzouanacou E Larralde O Watts TM Wilson V Forrester L Brickman JM 《Genesis (New York, N.Y. : 2000)》2007,45(6):353-360
Gene trapping is an insertional mutagenesis strategy that allows for simultaneous gene identification and mutation in embryonic stem (ES) cells. Gene trap vectors both disrupt coding sequence and report on the genes' endogenous expression. The most popular gene trap reporter to date combines beta-galactosidase expression with neomycin resistance in a fusion protein known as beta-geo. Here we describe a refinement to this reporter that also incorporates real time fluorescent readouts. We have constructed a series of gene trap vectors incorporating a novel tripartite fusion protein consisting of EGFP, beta-galactosidase, and the neomycin or hygromycin resistance activities. Our results indicate that these triple fusions can function efficiently as reporters of endogenous trapped gene expression and subcellular localization. We show that these fusion proteins constitute versatile gene trap reporters whose activity can be detected in real time by fluorescence and in fixed tissue with a sensitive enzymatic activity. 相似文献
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Themis-Dimitrios Kyprianou Hans O. Pörtner Andreas Anestis Basile Kostoglou Konstantinos Feidantsis Basile Michaelidis 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》2010,180(7):1005-1018
The winter syndrome in the gilthead sea bream Sparus aurata indicates that the species is exposed to critically low temperatures in Mediterranean aquaculture in winter. The present
study of metabolic patterns and molecular stress responses during cold exposure was carried out to investigate this “disease”,
in light of the recent concept of oxygen and capacity limited thermal tolerance. The metabolic profile of fuel oxidation was
examined by determining the activities of the enzymes hexokinase (HK), aldolase (Ald), pyruvate kinase (PK), l-lactate dehydrogenase (l-LDH), citrate synthase (CS), malate dehydrogenase (MDH) and 3-hydroxyacyl CoA dehydrogenase (HOAD) in heart, red and white
muscle after exposure to temperatures of 10, 14 and 18°C. Especially, the increase in LDH activity combined with the accumulation
of l-lactate in tissues indicates that temperatures below 14°C are critical for Sparus aurata and stimulate the anaerobic component of metabolism. Increase in the activity of HOAD suggests that oxidation of free fatty
acids might contribute to ATP turnover at low temperatures. The expression of Hsp70 and Hsp90 in all tissues examined revealed
a cellular stress response during cooling below 18°C. In the light of winter temperatures in S. aurata cultures around 10°C, our data suggest that the fish are exposed to stressful conditions at the low end of their thermal
tolerance window. These conditions likely impair the aerobic capacity of the fish, compromise the rates of growth and reproduction
and may contribute to elicit pathological conditions. 相似文献
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