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排序方式: 共有145条查询结果,搜索用时 15 毫秒
91.
Vattemi G Mechref Y Marini M Tonin P Minuz P Grigoli L Guglielmi V Klouckova I Chiamulera C Meneguzzi A Di Chio M Tedesco V Lovato L Degan M Arcaro G Lechi A Novotny MV Tomelleri G 《Molecular & cellular proteomics : MCP》2011,10(4):M110.002964
Mitochondrial diseases (MD) are heterogeneous disorders because of impairment of respiratory chain function leading to oxidative stress. We hypothesized that in MD the vascular endothelium may be affected by increased oxidative/nitrative stress causing a reduction of nitric oxide availability. We therefore, investigated the pathobiology of vasculature in MD patients by assaying the presence of 3-nitrotyrosine in muscle biopsies followed by the proteomic identification of proteins which undergo tyrosine nitration. We then measured the flow-mediated vasodilatation as a proof of altered nitric oxide generation/bioactivity. Here, we show that 3-nitrotyrosine staining is specifically located in the small vessels of muscle tissue and that the reaction is stronger and more evident in a significant percentage of vessels from MD patients as compared with controls. Eleven specific proteins which are nitrated under pathological conditions were identified; most of them are involved in energy metabolism and are located mainly in mitochondria. In MD patients the flow-mediated vasodilatation was reduced whereas baseline arterial diameters, blood flow velocity and endothelium-independent vasodilatation were similar to controls. The present results provide evidence that in MD the vessel wall is a target of increased oxidative/nitrative stress. 相似文献
92.
Grings M Tonin AM Knebel LA Zanatta A Moura AP Filho CS Wajner M Leipnitz G 《Molecular and cellular biochemistry》2012,366(1-2):335-343
Phytanic acid (Phyt) accumulates in tissues and biological fluids of patients affected by Refsum disease. Although cardiomyopathy is an important clinical manifestation of this disorder, the mechanisms of heart damage are poorly known. In the present study, we investigated the in vitro effects of Phyt on important parameters of oxidative stress in heart of young rats. Phyt significantly increased thiobarbituric acid-reactive substances levels (P < 0.001) and carbonyl formation (P < 0.01), indicating that this fatty acid induces lipid and protein oxidative damage, respectively. In contrast, Phyt did not alter sulfhydryl oxidation. Phyt also decreased glutathione (GSH) concentrations (P < 0.05), an important non-enzymatic antioxidant defense. Moreover, Phyt increased 2',7'-dichlorofluorescin oxidation (DCFH) (P < 0.01), reflecting increased reactive species generation. We also found that the induced lipid and protein oxidative damage, as well as the decreased GSH levels and increased DCFH oxidation provoked by this fatty acid were prevented or attenuated by the reactive oxygen species scavengers melatonin, trolox, and GSH, but not by the nitric oxide inhibitor N: (ω)-nitro-L: -arginine methyl ester, suggesting that reactive oxygen species were involved in these effects. Next, we verified that Phyt strongly inhibited NADH-cytochrome c oxidoreductase (complex I-III) activity (P < 0.001) in heart supernatants, and decreased membrane potential and the NAD(P)H pool in heart mitochondria, indicating that Phyt acts as a metabolic inhibitor and as an uncoupler of the electron transport chain. Therefore, it can be presumed that disturbance of cellular energy and redox homeostasis induced by Phyt may possibly contribute to the cardiomyopathy found in patients affected by Refsum disease. 相似文献
93.
Lagatolla C Skerlavaj S Dolzani L Tonin EA Monti Bragadin C Bosco M Rizzo R Giglio L Cescutti P 《FEMS microbiology letters》2002,209(1):99-106
Eleven strains of Burkholderia cepacia were isolated directly from clinical specimens: 10 from sputum of cystic fibrosis patients, and one from a vaginal swab. They were biochemically identified using API20NE and confirmed by a PCR-based assay. The genomovar characterisation obtained by specific PCR amplification revealed seven strains belonging to genomovar I, three belonging to genomovar IIIA and one belonging to genomovar IV. All isolates were also typed by ribotyping and random amplification of polymorphic DNA analysis. Some of the characterised strains were examined for the ability to produce exopolysaccharides, with the aim of correlating the genomovar with the exopolysaccharide structure. The polysaccharides were analysed by means of methylation analysis and 1H-NMR spectroscopy in order to determine structural similarities. It was shown that different strains are capable of producing chemically different polysaccharides. 相似文献
94.
95.
G Viale F Grassi M Pelagi R Alzani S Ménard S Miotti R Buffa A Gini A G Siccardi 《Journal of immunology (Baltimore, Md. : 1950)》1987,139(12):4250-4255
MBr1 is a murine monoclonal antibody, defining a saccharidic epitope [CaMBr1] of a human tissue-specific, tumor-associated globoside, present on the mammary carcinoma cell line MCF-7. The same epitope is shared by glycoproteins present on normal and neoplastic mammary epithelial cells, and by mucins from some ovarian cyst fluids. We have used MBr1 as the monoclonal antitumor antibody in an idiotypic sequence of immunizations in order to obtain and characterize "internal images" of the original epitope to be used as substitutes of the nominal antigen in serologic immunoassays. Two monoclonal anti-idiotypic antibodies (beta-1 and beta-2), which reacted with paratope-related idiotopes on MBr1, were obtained. The analysis of the antigenic and immunogenic properties of these molecules by both "antigen" and "antibody" competition assays provided evidence that both beta-1 and beta-2 bear "internal images" of the MBr1-defined epitope. Moreover, when injected in mice and rabbits both beta-1 and beta-2 induced anti anti-idiotypic antibodies, which mimicked MBr1 in binding MCF-7 as well as normal and neoplastic mammary gland epithelial cells. These data are discussed in terms of their possible application to the production of tumor-associated antigen substitutes and their use in serologic immunoassays. 相似文献
96.
97.
N. Rahman Fatima Abidi Deborah Ford Laura Arbour Elizabeth Rapley Patricia Tonin David Barton Gillian Batcup J. Berry Finbarr Cotter Val Davison Mary Gerrard Elizabeth Gray Richard Grundy Magdi Hanafy Derek King Ian Lewis Annette Ridolfi Luethy Lisa Madlensky Jill Mann Anne O’Meara Tony Oakhill Mark Skolnick Louise Strong Dick Variend Steven Narod Charles Schwartz Kathryn Pritchard-Jones Michael R. Stratton 《Human genetics》1998,103(5):547-556
A susceptibility gene for Wilms’ tumour (WT), designated FWT1, was previously mapped to chromosome 17q12–q21 by linkage analysis of a single family. We now confirm the existence of this
gene by analysis of additional cases in the original family (3-point LOD score=5.69), and by detecting strong evidence of
linkage to this region in an unrelated pedigree with seven cases of WT (3-point LOD score=2.56). Analysis of 11 smaller WT
families confirms that there is genetic heterogeneity in familial WT, as three families exhibit strong evidence against linkage
to FWT1. One of these was subsequently found to have a predisposing WT1 mutation. However, the other two families show evidence against both FWT1 and WT1, suggesting that at least one further familial WT gene exists. Analysis of the phenotype of 16 WT cases from the families
linked to FWT1 demonstrates that they present at a significantly older age and a significantly later stage than both sporadic WT and the
six cases from two families unlinked to either FWT1 or WT1. The results confirm the role of FWT1 in susceptibility to WT, provide strong evidence for genetic heterogeneity in familial WT and suggest there are phenotypic
differences between familial WT due to FWT1, familial WT due to other genes and non-familial WT.
Received: 24 April 1998 / Accepted: 8 July 1998 相似文献
98.
Camila Gazolla Volpiano Bruno Brito Lisboa Jackson Freitas Brilhante São José Andreia Mara Rotta de Oliveira Anelise Beneduzi Luciane Maria Pereira Passaglia Luciano Kayser Vargas 《Plant and Soil》2018,432(1-2):229-243
Aims
To identify Rhizobium strains’ ability to biocontrol Sclerotium rolfsii, a fungus that causes serious damage to the common bean and other important crops, 78 previously isolated rhizobia from common bean were assessed.Methods
Dual cultures, volatiles, indole-acetic acid (IAA), siderophore production and 16S rRNA sequencing were employed to select strains for pot and field experiments.Results
Thirty-three antagonistic strains were detected in dual cultures, 16 of which were able to inhibit ≥84% fungus mycelial growth. Antagonistic strains produced up to 36.5 μg mL?1 of IAA, and a direct correlation was verified between IAA production and mycelium inhibition. SEMIA 460 inhibited 45% of mycelial growth through volatile compounds. 16S rRNA sequences confirmed strains as Rhizobium species. In pot condition, common bean plants grown on S. rolfsii-infested soil and inoculated with SEMIA 4032, 4077, 4088, 4080, 4085, or 439 presented less or no disease symptoms. The most efficient strains under field conditions, SEMIA 439 and 4088, decreased disease incidence by 18.3 and 14.5% of the S. rolfsii-infested control.Conclusions
Rhizobium strains could be strong antagonists towards S. rolfsii growth. SEMIA 4032, 4077, 4088, 4080, 4085, and 439 are effective in the biological control of the collar rot of the common bean.99.
Mutations in BRCA1 and BRCA2 in breast cancer families: are there more breast cancer-susceptibility genes? 总被引:11,自引:3,他引:8 下载免费PDF全文
O M Serova S Mazoyer N Puget V Dubois P Tonin Y Y Shugart D Goldgar S A Narod H T Lynch G M Lenoir 《American journal of human genetics》1997,60(3):486-495
To estimate the proportion of breast cancer families due to BRCA1 or BRCA2, we performed mutation screening of the entire coding regions of both genes supplemented with linkage analysis of 31 families, 8 containing male breast cancers and 23 site-specific female breast cancer. A combination of protein-truncation test and SSCP or heteroduplex analyses was used for mutation screening complemented, where possible, by the analysis of expression level of BRCA1 and BRCA2 alleles. Six of the eight families with male breast cancer revealed frameshift mutations, two in BRCA1 and four in BRCA2. Although most families with female site-specific breast cancers were thought to be due to mutations in either BRCA1 or BRCA2, we identified only eight mutations in our series of 23 site-specific female breast cancer families (34%), four in BRCA1 and four in BRCA2. According to the posterior probabilities calculated for mutation-negative families, based on linkage data and mutation screening results, we would expect 8-10 site-specific female breast cancer families of our series to be due to neither BRCA1 nor BRCA2. Thus, our results suggest the existence of at least one more major breast cancer-susceptibility gene. 相似文献
100.
Fernanda da S. Moreira Pedro B. da Costa Rocheli de Souza Anelise Beneduzi Bruno B. Lisboa Luciano K. Vargas Luciane M. P. Passaglia 《Genetics and molecular biology》2016,39(1):111-121
In the pursuit of sustainable agriculture, bioinoculants usage as providers of a
crop''s needs is a method to limit environmental damage. In this study, a
collection of cultivable putative plant growth promoting (PGP) bacteria
associated with wheat crops was obtained and this bacterial sample was
characterized in relation to the functional diversity of certain PGP features.
The isolates were obtained through classical cultivation methods, identified by
partial 16S rRNA gene sequencing and characterized for PGP traits of interest.
Functional diversity characterization was performed using Categorical Principal
Component Analysis (CatPCA) and Multiple Correspondence Analysis (MCA). The most
abundant genera found among the 346 isolates were Pseudomonas,
Burkholderia, and Enterobacter. Occurrence of PGP
traits was affected by genus, niche, and sampling site. A large number of genera
grouped together with the ability to produce indolic compounds; phosphate
solubilization and siderophores production formed a second group related to
fewer genera, in which the genus Burkholderia has a great
importance. The results obtained may help future studies aiming prospection of
putative plant growth promoting bacteria regarding the desired organism and PGP
trait. 相似文献