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31.
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The sea water temperature off the Norwegian coast was lower in 1994 than in 1992 and 1993. RNA/DNA ratios of Norwegian spring spawning herring increased with increasing larval dry weight all years, except for larvae sampled south of 62°N in 1994. The RNA/DNA ratios indicated that each year, only a small portion ( 0.7%) of the larvae were starving. RNA/DNA ratios and temperature were negatively correlated in 1992, but in other years no significant correlations were found. Residuals of In RNA v . In DNA and In W v. L s were poorly correlated in all years, but residuals of In RNA v. In DNA and In DNA v. In W were negatively correlated in all years. Principal component analysis showed that the RNA/DNA ratio and DNA (% of weight) were correlated with different axes. Abundance data for herring at the early larval and 0-group stages in 1992–1994 indicated higher mortality in 1994 compared with the other years. The data do not indicate that average larval condition was poorer in 1994 than in other years. However, the variability in larval condition was higher in 1994 than in other years, and the condition of later larval stages was relatively lower in 1994 than in other years. 相似文献
33.
Ane Cecilie Kvernvik Clara Jule Marie Hoppe Evelyn Lawrenz Ondřej Prášil Michael Greenacre Józef Maria Wiktor Eva Leu 《Journal of phycology》2018,54(4):461-470
Arctic microalgae experience long periods of continuous darkness during the polar night, when they are unable to photosynthesize. Despite numerous studies on overwintering strategies, such as utilization of stored energy products, formation of resting stages, reduction of metabolic rates and heterotrophic lifestyles, there have been few attempts to assess the in situ physiological state and restoration of the photosynthetic apparatus upon re‐illumination. In this study, we found diverse and active marine phytoplankton communities during the polar night at 78°N. Furthermore, we observed rapid changes (≤20 min) in the efficiency of photosynthetic electron transport upon re‐illumination. High photosynthetic capacity and net primary production were established after 24 h of re‐illumination. Our results suggest that some Arctic autotrophs maintain fully functional photosystem II and downstream electron acceptors during the polar night even though the low in situ net primary production levels measured in January prove that light was not sufficient to support any measurable primary production. Due to low temperatures resulting in low respiratory rates as well as the absence of photodamage during the polar night, maintenance of basic photosynthetic machinery may actually pose relatively low metabolic costs for algal cells. This could allow Arctic microalgae to endure the polar night without the formation of dormant stages, enabling them to recover and take advantage of light immediately upon the suns return during the winter–spring transition. 相似文献
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Ane Larraaga-Vera Miguel Marco-Bonilla Raquel Largo Gabriel Herrero-Beaumont Arnzazu Mediero Bruce Cronstein 《Purinergic signalling》2021,17(4):591
Extracellular adenosine triphosphate (ATP) plays a central role in a wide variety of joint diseases. ATP is generated intracellularly, and the concentration of the extracellular ATP pool is determined by the regulation of its transport out of the cell. A variety of ATP transporters have been described, with connexins and pannexins the most commonly cited. Both form intercellular channels, known as gap junctions, that facilitate the transport of various small molecules between cells and mediate cell–cell communication. Connexins and pannexins also form pores, or hemichannels, that are permeable to certain molecules, including ATP. All joint tissues express one or more connexins and pannexins, and their expression is altered in some pathological conditions, such as osteoarthritis (OA) and rheumatoid arthritis (RA), indicating that they may be involved in the onset and progression of these pathologies. The aging of the global population, along with increases in the prevalence of obesity and metabolic dysfunction, is associated with a rising frequency of joint diseases along with the increased costs and burden of related illness. The modulation of connexins and pannexins represents an attractive therapeutic target in joint disease, but their complex regulation, their combination of gap-junction-dependent and -independent functions, and their interplay between gap junction and hemichannel formation are not yet fully elucidated. In this review, we try to shed light on the regulation of these proteins and their roles in ATP transport to the extracellular space in the context of joint disease, and specifically OA and RA. 相似文献
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Late Prehistoric Wood Use in an Andean Intermontane Valley. Economic Botany 59(4): 337-355, 2005. Data from carbonized wood remains from five late prehistoric sites (A.D. 1300–1530)
in the Upper Mantaro Valley, Peru, are presented. The wood assemblage recovered is diverse. We illustrate and describe the
twelve main morpho-types, one of which is more than likely to have been imported from the eastern slopes of the Andean mountains
into the Mantaro Valley. The Inka conquest of the area created a change in socio-political power and also brought about a
shift in the way wood species were utilized. From the Wanka II to the Inka occupations we find an increase in theBuddleja, Polylepis, Alnus, andColletia morpho-types: all of these species are reported as being cultivated by the Inka and possibly by pre-Inka inhabitants of the
valley. A quantitative analysis shows higher amounts of wood in the elite dwellings of both the Wanka II and Wanka III times,
suggesting a social aspect in the way this valuable Andean commodity was distributed and used.
Résumé El Uso de Madera Prehistorico Tardio en un Valle Andino. Fueron analizados los restos de madera provenientes de cinco sitios del período prehistorico tardio (1300–1530 d.C.) del Alto Valle Mantaro, Perú. La asamblea de moderns era muy variada Ilutramos y describimos las doce categorías taxonómicas prinicipales, una de las cuales probablamente fue importada desde las cuestas orientales de los Andes. La conquista Inkaica de la región efectuó un cambio de énfasis en los morfotipos de madera usados, con un aumento deBuddleja, Polylepis,Alnus, yColletia. Un análisis cuantitativo muestra una concentracion de maderas en las habitaciones de la clase élite, sugeriendo un aspecto social en el uso de la madera.相似文献
38.
Axel Gansmüller Maria Concistrè Neville McLean Ole G. Johannessen Ildefonso Marín-Montesinos Petra H.M. Bovee-Geurts Peter Verdegem Johan Lugtenburg Richard C.D. Brown Willem J. DeGrip Malcolm H. Levitt 《生物化学与生物物理学报:生物膜》2009,1788(6):1350-1357
Photoisomerization of the membrane-bound light receptor protein rhodopsin leads to an energy-rich photostate called bathorhodopsin, which may be trapped at temperatures of 120 K or lower. We recently studied bathorhodopsin by low-temperature solid-state NMR, using in situ illumination of the sample in a purpose-built NMR probe. In this way we acquired 13C chemical shifts along the retinylidene chain of the chromophore. Here we compare these results with the chemical shifts of the dark state chromophore in rhodopsin, as well as with the chemical shifts of retinylidene model compounds in solution. An earlier solid-state NMR study of bathorhodopsin found only small changes in the 13C chemical shifts upon isomerization, suggesting only minor perturbations of the electronic structure in the isomerized retinylidene chain. This is at variance with our recent measurements which show much larger perturbations of the 13C chemical shifts. Here we present a tentative interpretation of our NMR results involving an increased charge delocalization inside the polyene chain of the bathorhodopsin chromophore. Our results suggest that the bathochromic shift of bathorhodopsin is due to modified electrostatic interactions between the chromophore and the binding pocket, whereas both electrostatic interactions and torsional strain are involved in the energy storage mechanism of bathorhodopsin. 相似文献
39.
Ane Marcos-Carcavilla Carole Moreno Magdalena Serrano Pascal Laurent Edmond P. Cribiu Olivier Andréoletti Julien Ruesche Jean-Louis Weisbecker Jorge H. Calvo Katayoun Moazami-Goudarzi 《Cell stress & chaperones》2010,15(4):343-349
Susceptibility to scrapie is mainly controlled by point mutations at the PRNP locus. However, additional quantitative trait loci (QTL) have been identified across the genome including a region in OAR18.
The gene which encodes the inducible form of the cytoplasmic Hsp90 chaperone (HSP90AA1) maps within this region and seems to be associated with the resistance/susceptibility to scrapie in sheep. Here, we have
analyzed several polymorphisms which were previously described in the ovine HSP90AA1 5′ flanking region and in intron 10 in two naturally scrapie infected Romanov sheep populations. First, we have studied 58
ARQ/VRQ animals pertaining to the sire family where the QTL influencing scrapie incubation period in OAR18 was detected. We
have found a significant association between polymorphisms localized at −660 and −528 in the HSP90AA1 5′ flanking region and the scrapie incubation period. These two polymorphisms have also been studied in a second sample constituted
by 62 VRQ/VRQ sheep showing an extreme incubation period. Results are concordant with the first dataset. Finally, we have
studied the HSP90AA1 expression in scrapie and control animals (N = 41) with different HSP90AA1 genotypes by real time PCR on blood samples. The HSP90AA1 expression rate was equivalent in CC−600AA−528 and CG−600AG−528 scrapie resistant animals (ARR/ARR) and was higher in their CC−600AA−528 than in their CG−600AG−528 scrapie susceptible counterparts (VRQ/VRQ). Our results support the hypothesis that the ovine HSP90AA1 gene acts as a modulator of scrapie susceptibility, contributing to the observed differences in the incubation period of
scrapie infected animals with the same PRNP genotype. 相似文献
40.
Ane Landajuela Martha Braun Christopher D. A. Rodrigues Alejandro Martínez-Calvo Thierry Doan Florian Horenkamp Anna Andronicos Vladimir Shteyn Nathan D. Williams Chenxiang Lin Ned S. Wingreen David Z. Rudner Erdem Karatekin 《PLoS biology》2021,19(6)
Little is known about mechanisms of membrane fission in bacteria despite their requirement for cytokinesis. The only known dedicated membrane fission machinery in bacteria, fission protein B (FisB), is expressed during sporulation in Bacillus subtilis and is required to release the developing spore into the mother cell cytoplasm. Here, we characterized the requirements for FisB-mediated membrane fission. FisB forms mobile clusters of approximately 12 molecules that give way to an immobile cluster at the engulfment pole containing approximately 40 proteins at the time of membrane fission. Analysis of FisB mutants revealed that binding to acidic lipids and homo-oligomerization are both critical for targeting FisB to the engulfment pole and membrane fission. Experiments using artificial membranes and filamentous cells suggest that FisB does not have an intrinsic ability to sense or induce membrane curvature but can bridge membranes. Finally, modeling suggests that homo-oligomerization and trans-interactions with membranes are sufficient to explain FisB accumulation at the membrane neck that connects the engulfment membrane to the rest of the mother cell membrane during late stages of engulfment. Together, our results show that FisB is a robust and unusual membrane fission protein that relies on homo-oligomerization, lipid binding, and the unique membrane topology generated during engulfment for localization and membrane scission, but surprisingly, not on lipid microdomains, negative-curvature lipids, or curvature sensing.Little is known about how membrane fission occurs in bacteria; this study suggests that the membrane fission protein FisB exploits the unique cellular geometry encountered during sporulation to enable its localization to the fission site through a novel mechanism, where it catalyzes membrane scission. 相似文献