全文获取类型
收费全文 | 1961篇 |
免费 | 162篇 |
国内免费 | 2篇 |
出版年
2023年 | 10篇 |
2022年 | 12篇 |
2021年 | 49篇 |
2020年 | 29篇 |
2019年 | 43篇 |
2018年 | 46篇 |
2017年 | 45篇 |
2016年 | 70篇 |
2015年 | 103篇 |
2014年 | 117篇 |
2013年 | 144篇 |
2012年 | 178篇 |
2011年 | 141篇 |
2010年 | 107篇 |
2009年 | 102篇 |
2008年 | 110篇 |
2007年 | 127篇 |
2006年 | 106篇 |
2005年 | 105篇 |
2004年 | 84篇 |
2003年 | 68篇 |
2002年 | 71篇 |
2001年 | 19篇 |
2000年 | 16篇 |
1999年 | 16篇 |
1998年 | 16篇 |
1997年 | 10篇 |
1996年 | 8篇 |
1995年 | 10篇 |
1994年 | 6篇 |
1993年 | 6篇 |
1991年 | 4篇 |
1990年 | 5篇 |
1989年 | 5篇 |
1987年 | 8篇 |
1986年 | 9篇 |
1985年 | 5篇 |
1984年 | 10篇 |
1983年 | 7篇 |
1980年 | 4篇 |
1978年 | 6篇 |
1974年 | 11篇 |
1973年 | 5篇 |
1972年 | 9篇 |
1971年 | 6篇 |
1970年 | 6篇 |
1969年 | 6篇 |
1968年 | 6篇 |
1967年 | 8篇 |
1965年 | 7篇 |
排序方式: 共有2125条查询结果,搜索用时 46 毫秒
61.
62.
The ability to model algal productivity under transient conditions of light intensity and temperature is critical for assessing the profitability and sustainability of full-scale algae cultivation outdoors. However, a review of over 40 modeling approaches reveals that most of the models hitherto described in the literature have not been validated under conditions relevant to outdoor cultivation. With respect to light intensity, we therefore categorized and assessed these models based on their theoretical ability to account for the light gradients and short light cycles experienced in well-mixed dense outdoor cultures. Type I models were defined as models predicting the rate of photosynthesis of the entire culture as a function of the incident or average light intensity reaching the culture. Type II models were defined as models computing productivity as the sum of local productivities within the cultivation broth (based on the light intensity locally experienced by individual cells) without consideration of short light cycles. Type III models were then defined as models considering the impacts of both light gradients and short light cycles. Whereas Type I models are easy to implement, they are theoretically not applicable to outdoor systems outside the range of experimental conditions used for their development. By contrast, Type III models offer significant refinement but the complexity of the inputs needed currently restricts their practical application. We therefore propose that Type II models currently offer the best compromise between accuracy and practicability for full scale engineering application. With respect to temperature, we defined as “coupled” and “uncoupled” models the approaches which account and do not account for the potential interdependence of light and temperature on the rate of photosynthesis, respectively. Due to the high number of coefficients of coupled models and the associated risk of overfitting, the recommended approach is uncoupled models. Most of models do not include the modeling of endogenous respiration and the modeling of light and temperature acclimation in spite of their potential effect on productivity. 相似文献
63.
Thankaraj Salammal Mariashibu Kondeti Subramanyam Muthukrishnan Arun Subramanian Mayavan Manoharan Rajesh Jeevaraj Theboral Markandan Manickavasagam Andy Ganapathi 《Acta Physiologiae Plantarum》2013,35(1):41-54
For the first time we have developed a reliable and efficient vacuum infiltration-assisted Agrobacterium-mediated genetic transformation (VIAAT) protocol for Indian soybean cultivars and recovered fertile transgenic soybean plants through somatic embryogenesis. Immature cotyledons were used as an explant and three Agrobacterium tumefaciens strains (EHA 101, EHA 105, and KYRT 1) harbouring the binary vector pCAMBIA1301 were experimented in the co-cultivation. The immature cotyledons were pre-cultured in liquid somatic embryo induction medium prior to vacuum infiltration with the Agrobacterium suspension and co-cultivated for 3 days on co-cultivation medium containing 50 mg l?1 citric acid, 100 µM acetosyringone, and 100 mg l?1 l-cysteine. The transformed somatic embryos were selected in liquid somatic embryo induction medium containing 10 mg l?1 hygromycin and the embryos were germinated in basal medium containing 20 mg l?1 hygromycin. The presence and integration of the hpt II and gus genes into the soybean genome were confirmed by GUS histochemical assay, polymerase chain reaction, and Southern hybridization. Among the different combinations tested, high transformation efficiency (9.45 %) was achieved when immature cotyledons of cv. Pusa 16 were pre-cultured for 18 h and vacuum infiltrated with Agrobacterium tumefaciens KYRT 1 for 2 min at 750 mm of Hg. Among six Indian soybean cultivars tested, Pusa 16 showed highest transformation efficiency of 9.45 %. The transformation efficiency of this method (VIAAT) was higher than previously reported sonication-assisted Agrobacterium-mediated transformation. These results suggest that an efficient Agrobacterium-mediated transformation protocol for stable integration of foreign genes into soybean has been developed. 相似文献
64.
Estela Puchulu-Campanella Haiyan Chu David J. Anstee Jacob A. Galan W. Andy Tao Philip S. Low 《The Journal of biological chemistry》2013,288(2):848-858
Glycolytic enzymes (GEs) have been shown to exist in multienzyme complexes on the inner surface of the human erythrocyte membrane. Because no protein other than band 3 has been found to interact with GEs, and because several GEs do not bind band 3, we decided to identify the additional membrane proteins that serve as docking sites for GE on the membrane. For this purpose, a method known as “label transfer” that employs a photoactivatable trifunctional cross-linking reagent to deliver a biotin from a derivatized GE to its binding partner on the membrane was used. Mass spectrometry analysis of membrane proteins that were biotinylated following rebinding and photoactivation of labeled GAPDH, aldolase, lactate dehydrogenase, and pyruvate kinase revealed not only the anticipated binding partner, band 3, but also the association of GEs with specific peptides in α- and β-spectrin, ankyrin, actin, p55, and protein 4.2. More importantly, the labeled GEs were also found to transfer biotin to other GEs in the complex, demonstrating for the first time that GEs also associate with each other in their membrane complexes. Surprisingly, a new GE binding site was repeatedly identified near the junction of the membrane-spanning and cytoplasmic domains of band 3, and this binding site was confirmed by direct binding studies. These results not only identify new components of the membrane-associated GE complexes but also provide molecular details on the specific peptides that form the interfacial contacts within each interaction. 相似文献
65.
Zhao Shan Qinglin Han Jia Nie Xuezhi Cao Zuojia Chen Shuying Yin Yayi Gao Fang Lin Xiaohui Zhou Ke Xu Huimin Fan Zhikang Qian Bing Sun Jin Zhong Bin Li Andy Tsun 《The Journal of biological chemistry》2013,288(49):35093-35103
Although lysine methylation is classically known to regulate histone function, its role in modulating antiviral restriction factor activity remains uncharacterized. Interferon-induced transmembrane protein 3 (IFITM3) was found monomethylated on its lysine 88 residue (IFITM3-K88me1) to reduce its antiviral activity, mediated by the lysine methyltransferase SET7. Vesicular stomatitis virus and influenza A virus infection increased IFITM3-K88me1 levels by promoting the interaction between IFITM3 and SET7, suggesting that this pathway could be hijacked to support infection; conversely, IFN-α reduced IFITM3-K88me1 levels. These findings may have important implications in the design of therapeutics targeting protein methylation against infectious diseases. 相似文献
66.
67.
Andy Powell Craig Baker-Austin Sariqa Wagley Amanda Bayley Rachel Hartnell 《Microbial ecology》2013,65(4):924-927
Vibrio parahaemolyticus is a Gram-negative, halophilic bacterium found commonly in temperate and warm estuarine waters worldwide. V. parahaemolyticus is considered an emerging bacterial pathogen in Europe and has been responsible for several recent seafood-associated outbreaks. During ad hoc testing of raw shellfish produce in May 2012, pandemic group (O3:K6) V. parahaemolyticus was isolated from Pacific oysters (Crassostrea gigas), harvested in Southern England. Follow-on testing of water and shellfish, encompassing a small number geographically diverse sites, also retrieved pandemic group isolates. These strains are amongst the most northerly pandemic strains described to date and represent the first instance of pandemic V. parahaemolyticus isolated in the UK, highlighting the expanding geographical distribution of these foodborne pathogens in the environment. 相似文献
68.
Andy Gardner 《Biology & philosophy》2013,28(5):787-791
My understanding is that proximate explanations concern adaptive mechanism and that ultimate explanations concern adaptive rationale. Viewed in this light, the two kinds of explanation are quite distinct, but they interact in a complementary way to give a full understanding of biological adaptations. In contrast, Laland et al. (2013)—following a literal reading of Mayr (Science 134:1501–1506, 1961)—have characterized ultimate explanations as concerning any and all mechanisms that have operated over the course of an organism’s evolutionary history. This has unfortunate consequences, such as allowing random drift to form the basis for ultimate explanations, and allowing proximate and ultimate explanations to bleed into each other until their distinction is meaningless. Here, I suggest Laland et al’s explanatory framework of “reciprocal causation” is not conducive to successful biological science, and that they have misunderstood key elements of the theory of Darwinian adaptation. 相似文献
69.
Capsule A newly created wetland has been beneficial for the breeding of five heron species and for the settlement and expansion of Glossy Ibises in the region. Aim To determine whether created wetland would lead to the establishment of a heron and ibis breeding colony, and if so, to determine its effects on the distribution of these species in the wider region. Methods Number of pairs breeding at the new wetland and in the region were estimated annually from 1996 to 2008. We investigated whether the growth of the new colony was explained by redistribution of breeding pairs within the region. Results Purple Herons, Squacco Herons and Glossy Ibises colonised the new wetland in 1996. Black‐crowned Night Herons settled in 1998, while Cattle and Little Egrets were the last species to establish in the mixed colony in 2001. The population increase was particularly marked for Glossy Ibises (which had bred in Doñana only sporadically through the 20th century). Our findings suggest that simple redistribution of pairs does not explain the formation and growth of the new colony. Since the first year of establishment numbers of Glossy Ibises and Purple Herons have shown a significant growth trend at both the new colony and in Doñana. Conclusion The creation and management of a new wetland has been successful for the conservation of heron and egret species and promoted the expansion of the Glossy Ibis population across the western Mediterranean region. 相似文献
70.
Species traits explain variation in detectability of UK birds 总被引:1,自引:0,他引:1
Alison Johnston Stuart E. Newson Kate Risely Andy J. Musgrove Dario Massimino Stephen R. Baillie 《Bird Study》2013,60(3):340-350
Capsule Heterogeneous detectability amongst species may impact multi-species bird surveys and if not accounted for, may bias community level conclusions. Estimates of detectability were produced for 195 UK bird species, and detectability was significantly affected by bird size, diet and habitat specialization.Aims To estimate detectability and understand which species traits may impact detectability.Methods We estimated the detectability of 195 species of birds in the UK using distance sampling methods and examined the average detectability of genetically related groups. We tested the significance of species traits in describing variation in detectability, whilst controlling for phylogenetic relationships.Results Passeriformes had the lowest median detectability of 0.37 and Charadriiformes the highest median detectability of 0.65, of the seven largest orders considered. Species most associated with closed habitats such as woodland and urban areas had the lowest detectability. Smaller species had lower detectability than larger species.Conclusion Heterogeneity in species detectability could lead to biased conclusions, particularly when calculating multi-species indices such as species richness or diversity. Accounting for detectability will be most important in studies that cover a wide range of habitat types or a diverse spread of taxa. 相似文献