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Flavoridin and echistatin, isolated from the venom of Trimeresurus flavoviridis and Echis carinatus, respectively, belong to the disintegrin family of integrin beta 1 and beta 3 inhibitors of low molecular weight RGD-containing, cysteine-rich peptides. Since disulfide bonds are critical for expression of biological activity, we sought to determine their location in these two proteins. In flavoridin, direct evidence for the existence of linkage between Cys4-Cys19 and between Cys45 and Cys64 was obtained by analysis of proteolytic products, and indirect evidence suggests links between Cys6-Cys14 and Cys13-Cys36. In echistatin, links between Cys8-Cys37 and Cys20-Cys39 were identified by direct chemical analysis. 相似文献
88.
Andrew M. Smith 《Journal of experimental marine biology and ecology》1992,160(2):205-220
The attachment mechanism used by limpets in the rocky, wave-swept intertidal zone of California was determined during high tide and low tide. The two mechanisms that limpets are known to use, suction and glue-like adhesion, were distinguished by measuring the limpets' attachment forces in shear and by staining for glue-like residues where the limpets had been attached. The results show that ≈ 73% of limpets at high tide use suction, while the rest use glue-like adhesion. Conversely, ≈ 75% of limpets at low tide use glue-like adhesion, while the rest use suction. The normal tenacity of limpets was also measured at high and low tide. The mean tenacity at high tide was significantly less than that at low tide. From these data it was estimated that the mean tenacity of glue-like adhesion is ≈ 0.23 MN·m−2 and the mean tenacity of suction adhesion is ≈ 0.09 MN·m−2. It is hypothesized that the cycle of alternating attachment mechanisms is linked to the limpets foraging cycles. 相似文献
89.
Summary Culture conditions have been optimised to enable resting cell cultures ofAcinetobacter calcoaceticus NCIMB 9871 to selectively undertake either oxidative or reductive biotransformations of various bicyclic ketones. 相似文献
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Porphyrins and corrins have been immobilized onto solid-phase supports and used to dehalogenate organohalides under reducing conditions. One such system has been used to dehalogenate an aqueous solution (10 ppm) of lindane for over 90 days at over 98% efficiency and for a further 150 days in excess of 65% efficiency. Dichloromethane has been shown to be effectively dehalogenated when supplied to immobilized systems in gaseous form.
Correspondence to: T. S. Marks 相似文献