排序方式: 共有153条查询结果,搜索用时 15 毫秒
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Isabela T. Pereira Edneia A. S. Ramos Erico T. Costa Anamaria A. Camargo Graciele C. M. Manica Liliane M. B. Klassen Andressa Chequin Karin Braun-Prado Fábio de O. Pedrosa Emanuel M. Souza Fabricio F. Costa Giseli Klassen 《PloS one》2014,9(9)
Metastasis accounts for more than 90% of cancer deaths. Cells from primary solid tumors may invade adjacent tissues and migrate to distant sites where they establish new colonies. The tumor microenvironment is now recognized as an important participant in the signaling that induces cancer cell migration. An essential process for metastasis is extracellular matrix (ECM) degradation by metalloproteases (MMPs), which allows tumor cells to invade local tissues and to reach blood vessels. The members of this protein family include gelatinase A, or MMP-2, which is responsible for the degradation of type IV collagen, the most abundant component of the basal membrane, that separates epithelial cells in the stroma. It is known that fibronectin is capable of promoting the expression of MMP-2 in MCF7 breast cancer cells in culture. In addition, it was already shown that the MMP2 gene expression is regulated by epigenetic mechanisms. In this work, we showed that fibronectin was able to induce MMP2 expression by 30% decrease in its promoter methylation. In addition, a histone marker for an open chromatin conformation was significantly increased. These results indicate a new role for fibronectin in the communication between cancer cells and the ECM, promoting epigenetic modifications. 相似文献
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Vendruscolo Raquel Guidetti Fernandes Andressa Silva Fagundes Mariane Bittencourt Zepka Leila Queiroz de Menezes Cristiano Ragagnin Jacob–Lopes Eduardo Wagner Roger 《Journal of applied phycology》2021,33(4):1987-1997
Journal of Applied Phycology - There are still limitations in the pigment extraction methods used in microalgae biomass, especially for laboratory scale. This work aimed to develop a simple method... 相似文献
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Carolina V Morgante Patricia M Guimarães Andressa CQ Martins Ana CG Araújo Soraya CM Leal-Bertioli David J Bertioli Ana CM Brasileiro 《BMC research notes》2011,4(1):1-11
Background
Molecular genetic studies on rare tumour entities, such as bone tumours, often require the use of decalcified, formalin-fixed, paraffin-embedded tissue (dFFPE) samples. Regardless of which decalcification procedure is used, this introduces a vast breakdown of DNA that precludes the possibility of further molecular genetic testing. We set out to establish a robust protocol that would overcome these intrinsic hurdles for bone tumour research.Findings
The goal of our study was to establish a protocol, using a modified DNA isolation procedure and quality controls, to select decalcified samples suitable for array-CGH testing. Archival paraffin blocks were obtained from 9 different pathology departments throughout Europe, using different fixation, embedding and decalcification procedures, in order to preclude a bias for certain lab protocols. Isolated DNA samples were subjected to direct chemical labelling and enzymatic labelling systems and were hybridised on a high resolution oligonucleotide chip containing 44,000 reporter elements. Genomic alterations (gains and losses) were readily detected in most of the samples analysed. For example, both homozygous deletions of 0.6 Mb and high level of amplifications of 0.7 Mb were identified.Conclusions
We established a robust protocol for molecular genetic testing of dFFPE derived DNA, irrespective of fixation, decalcification or sample type used. This approach may greatly facilitate further genetic testing on rare tumour entities where archival decalcified, formalin fixed samples are the only source. 相似文献46.
Thaddeus Gregory Blanchette Ana Paula Silva Andressa Raylane Bento 《Dialectical Anthropology》2013,37(2):195-227
Based upon the contradictory definitions of the crime, the Brazilian movement against trafficking in persons situates itself as a “struggle against modern slavery.” Within this moralistic context, the movement has frequently utilized invented statistics and apocalyptic declarations regarding trafficking in order to achieve greater “advocacy value” among members of the Brazilian public. A key component of this discursive formation has been the creation and promulgation of a mythological view of a “typical” trafficking victim’s experience: what we call “The Myth of Maria, an exemplary trafficking victim.” The present article seeks to follow the history of the Myth of Maria, developing an initial chronology mapped out and analyzed by Adriana Piscitelli in 2004 and extending this into the post-2006 period when Brazil established its first national policies and plans to combat trafficking in persons. We then analyze how the myth ignores many of the realities revealed by the past decade of ethnographic research into trafficking in Brazil. Finally, we conclude with a structuralist hypothesis (drawn from the field of feminist anthropology) regarding the Myth’s continuing unabated popularity among almost all actors in the political field of anti-trafficking policy. 相似文献
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Cioato Stefania Giotti Medeiros Liciane Fernandes Lopes Bettega Costa de Souza Andressa Medeiros Helouise Richardt Assumpção José Antônio Fagundes Caumo Wolnei Roesler Rafael Torres Iraci L. S. 《Purinergic signalling》2020,16(4):573-584
This study aimed to evaluate the effect of a single administration of IB-MECA, an A3 adenosine receptor agonist, upon the nociceptive response and central biomarkers of rats submitted to chronic pain models. A total of 136 adult male Wistar rats were divided into two protocols: (1) chronic inflammatory pain (CIP) using complete Freund’s adjuvant and (2) neuropathic pain (NP) by chronic constriction injury of the sciatic nerve. Thermal and mechanical hyperalgesia was measured using von Frey (VF), Randal-Selitto (RS), and hot plate (HP) tests. Rats were treated with a single dose of IB-MECA (0.5 μmol/kg i.p.), a vehicle (dimethyl sulfoxide—DMSO), or positive control (morphine, 5 mg/kg i.p.). Interleukin 1β (IL-1β), brain-derived neurotrophic factor (BDNF), and nerve growth factor (NGF) levels were measured in the brainstem and spinal cord using enzyme-linked immunosorbent assay (ELISA). The establishment of the chronic pain (CIP or NP) model was observed 14 days after induction by a decreased nociceptive threshold in all three tests (GEE, P < 0.05). The antinociceptive effect of a single dose of IB-MECA was observed in both chronic pain models, but this was more effective in NP model. There was an increase in IL-1β levels promoted by CIP. NP model promoted increase in the brainstem BDNF levels, which was reversed by IB-MECA 相似文献
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Klanovicz Natalia Camargo Aline Frumi Stefanski Fábio Spitza Zanivan Jessica Scapini Thamarys Pollon Rafaela Warken Andressa Paliga Letícia Preczeski Karina Paula Ribeiro Anderson André Genro Alves Garda-Buffon Jaqueline Fongaro Gislaine Treichel Helen 《Bioprocess and biosystems engineering》2020,43(2):261-272
Bioprocess and Biosystems Engineering - Enzymes are becoming tools in industrial processes because of several advantages, including activity in mild environmental conditions, and high specificity.... 相似文献
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Ariane Fernanda da Silva Rodrigo Juliano Oliveira Andressa Megumi Niwa Gláucia Fernanda Rocha D’Epiro Lúcia Regina Ribeiro Mário Sérgio Mantovani 《Cytotechnology》2013,65(1):41-48
β-glucan is an important polysaccharide due to its medicinal properties of stimulating the immune system and preventing chronic diseases such as cancer. The aim of the present study was to determine the anticlastogenic effect of β-glucan in cells exposed to ultraviolet radiation (UV). Chromosome aberration assay was performed in drug-metabolizing cells (HTC) and non drug-metabolizing cells (CHO-K1 and repair-deficient CHO-xrs5), using different treatment protocols. Continuous treatment (UV + β-glucan) was not effective in reducing the DNA damage only in CHO-xrs5 cells. However, the pre-treatment protocol (β-glucan before UV exposition) was effective in reducing DNA damage only in CHO-K1 cells. In post-treatment (β-glucan after UV exposition) did not show significative anticlastogenic effects, although there was a tendency toward prevention. The data suggest that β-glucan has more than one action mechanism, being capable of exerting desmutagenic as well as bio-antimutagenic action. The findings also suggest that the presence of the xenobiotic metabolizing system can reduce the chemopreventive capacity of β-glucan. Therefore, these results indicate that β-glucan from Saccharomyces cerevisiae can be used in the prevention and/or reduction of DNA damage. 相似文献