排序方式: 共有153条查询结果,搜索用时 15 毫秒
31.
Andressa C. S. Nakagawa Haruka Itoyama Yuri Ariyoshi Nobuyuki Ario Yuki Tomita Yukari Kondo Mari Iwaya-Inoue Yushi Ishibashi 《Acta Physiologiae Plantarum》2018,40(6):111
Soybean seeds have high lipid and protein contents. Adverse environmental conditions restrict seed yield and quality. We examined the changes in storage compounds caused by drought stress from R5 stage (beginning seed growth stage). Under drought stress, contents of lipid in seed were remarkably low compared to control at 24 and 29 days after treatment. Protein contents in seed were immediately decreased after water deficit treatment. On the other hand, soluble sugar contents in seed were increased by drought stress. Drought stress decreased the expression of genes involved in lipid biosynthesis (PK, BCCP2, and KAS1) and increased the genes expression involved in lipid degradation (ACX2, MS, and PEPCK). These results suggest that the increasing of sugar content in seed under drought stress was complemented by degradation of lipids. The expressions of genes encoding storage protein (Gy4 and β-conglycinin) were also decreased by drought stress. This study showed how drought stress during seed filling affects seed quality, especially lipid and protein contents, that may facilitate further research on seed storage compounds metabolism under environmental stresses. 相似文献
32.
Andriéli Borges Santos Andressa Silva Fernandes Roger Wagner Eduardo Jacob-Lopes Leila Queiroz Zepka 《Journal of applied phycology》2016,28(3):1561-1570
The objective of this study was to investigate the volatile organic compounds (VOCs) produced from heterotrophic cultivation of the cyanobacterium Phormidium autumnale with different sources of monosaccharides. The volatiles were isolated by headspace solid-phase micro-extraction in different residence times, separated by gas chromatography, and identified by mass spectrometry (SPME-GC/MS). The profile of volatiles contained a total of 44 volatile compounds when P. autumnale was grown heterotrophically on glucose and 35 when grown on fructose. A combined total of 68 compounds was identified and 11 volatiles were common to both extracts. The compound 3-methyl-butanol was identified among the major volatile compounds formed, reaching a concentration of 141.5 μg mg?1 dry weight for the glucose-grown cultures and 69.5 μg mg?1 for the fructose-grown cultures after 144 h. Many of the compounds detected during heterotrophic cultivation originated from terpenoids (β-ionone, β-cyclocitral, and 5,6-epoxy-β-ionone), fatty acids (hexanol, hexanal), or the 2-keto acid pathway (3-methyl-butanol, propanol, butanol). 相似文献
33.
João Paulo P. Rosa Dayane F. Rodrigues Andressa Silva Mário Antônio de Moura Simim Varley T. Costa Franco Noce 《Chronobiology international》2016,33(4):435-440
The organizing committee of the 2016 Rio Olympic Games recently announced that some of the preliminary and final competitions will be held at night. The present article discusses the potential harmful effects of these late-night competitions on sleep, circadian rhythms and athletic performance during the Olympic Games. Specifically, night-time competition could lead to injury and may compromise an athlete’s decision-making, attentional, physiological and other processes. Consequently, these impacts could negatively affect the performance of athletes and their teams. Thus, it is suggested that technical commissions take special care when creating strategies to minimize harm to the athletes by considering factors such as light exposure, melatonin intake, sleep hygiene and scheduled naps, and training at local competition time. Furthermore, it is necessary for specialists in chronobiology and sleep to engage with members of the national teams to develop an activity schedule for physical, technical, tactical and psychological preparation that accounts for circadian rhythms, thereby creating the best possible environment for the athletes to achieve their ideal performance. 相似文献
34.
Maria Carolina Quecine Thiago Falda Leite Andressa Peres Bini Thais Regiani Lívia Maria Franceschini Ilara Gabriela Frasson Budzinski Felipe Garbelini Marques M?nica Teresa Veneziano Labate Simone Guidetti-Gonzalez David Henry Moon Carlos Alberto Labate 《PloS one》2016,11(1)
Puccinia psidii sensu lato (s.l.) is the causal agent of eucalyptus and guava rust, but it also attacks a wide range of plant species from the myrtle family, resulting in a significant genetic and physiological variability among populations accessed from different hosts. The uredospores are crucial to P. psidii dissemination in the field. Although they are important for the fungal pathogenesis, their molecular characterization has been poorly studied. In this work, we report the first in-depth proteomic analysis of P. psidii s.l. uredospores from two contrasting populations: guava fruits (PpGuava) and eucalyptus leaves (PpEucalyptus). NanoUPLC-MSE was used to generate peptide spectra that were matched to the UniProt Puccinia genera sequences (UniProt database) resulting in the first proteomic analysis of the phytopathogenic fungus P. psidii. Three hundred and fourty proteins were detected and quantified using Label free proteomics. A significant number of unique proteins were found for each sample, others were significantly more or less abundant, according to the fungal populations. In PpGuava population, many proteins correlated with fungal virulence, such as malate dehydrogenase, proteossomes subunits, enolases and others were increased. On the other hand, PpEucalyptus proteins involved in biogenesis, protein folding and translocation were increased, supporting the physiological variability of the fungal populations according to their protein reservoirs and specific host interaction strategies. 相似文献
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37.
Chiarella AP Arruda C Pina A Costa TA Ferreira RC Calich VL 《Microbes and infection / Institut Pasteur》2007,9(9):1078-1088
Protective immunity in paracoccidioidomycosis (PCM) is believed to be mediated by cellular immunity, but the role of T cell subsets has never been investigated. The aim of this study was to characterize the function of CD4+ and CD8+ T cells in the immunity developed by susceptible, intermediate and resistant mice after P. brasiliensis infection. In susceptible mice, depletion of CD4+ T cells did not alter disease severity and anergy of cellular immunity but diminished antibody production. Anti-CD8 treatment led to increased fungal loads, but restored DTH reactivity. In resistant mice, both CD4+ and CD8+ T cells control fungal burdens and cytokines although only the former regulate DTH reactions and antibody production. In the intermediate strain, deficiency of whole T and CD8+ T cells but not of CD4+ T or B cells led to increased mortality rates. Thus, in pulmonary PCM: (a) irrespective of the host susceptibility pattern, fungal loads are mainly controlled by CD8+ T cells, whereas antibody production and DTH reactions are regulated by CD4+ T cells; (c) CD4+ T cells play a protective role in the resistant and intermediate mouse strains, whereas in susceptible mice they are deleted or anergic; (d) genetic resistance to PCM is associated with concomitant CD4+ and CD8+ T cell immunity secreting type 1 and type 2 cytokines. 相似文献
38.
Cláudia Funchal Carlos Augusto Souza Carvalho Tanise Gemelli Andressa S. Centeno Robson Brum Guerra Mirian Salvador Caroline Dani Adriana Coitinho Rosane Gomez 《Cellular and molecular neurobiology》2010,30(7):1135-1142
Organotellurium compounds have been synthesized since 1840, but pharmacological and toxicological studies about them are still
incipient. Therefore, the objective of this study was to verify the effect of acute administration of the organochalcogen
3-butyl-1-phenyl-2-(phenyltelluro)oct-en-1-one on some parameters of oxidative stress in the brain of 30-day-old rats. Animals
were treated intraperitoneally with a single dose of the organotellurium (125, 250, or 500 μg/kg body weight) and sacrificed
60 min after the injection. The cerebral cortex, the hippocampus, and the cerebellum were dissected and homogenized in KCl.
Afterward, thiobarbituric acid reactive substances (TBARS), carbonyl, sulfhydryl, catalase (CAT), superoxide dismutase (SOD),
nitric oxide (NO) formation, and hydroxyl radical production were measured in the brain. The organotellurium enhanced TBARS
in the cerebral cortex and the hippocampus, and increased protein damage (carbonyl) in the cerebral cortex and the cerebellum.
In contrast, the compound provoked a reduced loss of thiol groups measured by the sulfhydryl assay in all the tissues studied.
Furthermore, the activity of the antioxidant enzyme CAT was reduced by the organochalcogen in the cerebral cortex and the
cerebellum, and the activity of SOD was inhibited in all the brain tissues. Moreover, NO production was increased in the cerebral
cortex and the cerebellum by this organochalcogen, and hydroxyl radical formation was also enhanced in the cerebral cortex.
Our findings indicate that this organotellurium compound induces oxidative stress in the brain of rats, corroborating that
this tissue is a potential target for organochalcogen action. 相似文献
39.
Patricia Andressa de Almeida Buranello Maria Raquel Isnard Moulin Devandir Antonio Souza Jr Maria Célia Jamur Maria Cristina Roque-Barreira Constance Oliver 《PloS one》2010,5(3)
Background
The D-mannose binding lectin ArtinM is known to recruit neutrophils, to degranulate mast cells and may have potential therapeutic applications. However, the effect of ArtinM on mast cell recruitment has not been investigated.Methodology
Male Wistar rats were injected i.p. with ArtinM or ConA (control). The ability of the lectin to degranulate peritoneal and mesenteric mast cells was examined. Recruitment of mast cells to the peritoneal cavity and mesentery after ArtinM injection was examined with or without depletion of peritoneal mast cells by distilled water.Results
ArtinM degranulated both peritoneal and mesentery mast cells in vitro. Three days after i.p. injection of the lectin there were reduced numbers of mast cells in the peritoneal lavage, while at 7 days post injection of ArtinM, the number of peritoneal mast cells was close to control values. Since immature mast cells are recruited from the bone marrow, the effect of the lectin on bone marrow mast cells was examined. Injection of ArtinM resulted in an increased number of mast cells in the bone marrow. To determine if degranulation of mast cells in the peritoneal cavity was required for the increase in bone marrow mast cells, the peritoneal cavity was depleted of mast cells with ultrapure water. Exposure to ArtinM increased the number of mast cells in the bone marrow of rats depleted of peritoneal mast cells.Conclusions
The ArtinM induced recruitment of mast cells from the bone marrow to the peritoneal cavity may partially explain the therapeutic actions of ArtinM. 相似文献40.
Andressa Carneiro do Nascimento José Montalva Mariano Lucia Daniel de Paiva Silva 《Journal of Applied Entomology》2023,147(1):23-35
The introduction of exotic species into native ecosystems can be a cause for concern when those species are invasive. Invasive species cause ecological problems and have socio-cultural impacts on human health and the economy; for example, invasive bees may negatively impact their introduced ecosystem by spreading diseases or outcompeting native pollinators. Xylocopa spp. bees are diverse and distributed throughout the Neotropics. However, Xylocopa augusti (Lepeletier, 1841) and Xylocopa splendidula (Lepeletier, 1841) are not native to Mediterranean Chile. This study aimed to evaluate the invasive potential of these exotic species and predict the potential macroecological effects of their invasions. We also aimed to pinpoint possible distributions for these species throughout South America. We correlated biogeographic occurrence data with climatic variables for each species to model their potential distribution in both current and future scenarios. The models provide strong evidence that both species are changing their distributions: their ranges are expanding towards western South America, particularly Bolivia, Chile and Peru. We demonstrate an increase in niche overlap between these species and show there are new geographic areas vulnerable to the establishment of these invasive bees under current and future climate conditions. These data suggest that these bees may adapt their geographic distribution as the climate changes and pose a threat to native pollinators in new geographic areas. 相似文献