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91.
João Paulo Rodrigues Martins Luiz Carlos de Almeida Rodrigues Lorenzo Toscano Conde Andreia Barcelos Passos Lima Gontijo Antelmo Ralph Falqueto 《Plant biosystems》2020,154(1):87-99
AbstractUnder in vitro culture conditions, plants may present physiological and anatomical disorders, which can interfere negatively after ex vitro transfer. The aim of this investigation was to analyze the impacts of natural ventilation and sucrose supply on the anatomy and physiology of Vriesea imperialis. Plants previously grown in vitro were transferred to culture medium containing 0, 15, 30 or 45?g L?1 sucrose. Three different culture container sealing systems were tested: lids with a green filter (81.35 gas exchanges per day), yellow filter (13.09 gas exchanges per day) or lids with a yellow filter covered with three layers of transparent polyvinylchloride (PVC) film (blocking fluent gas exchange). Sucrose concentrations influenced thickness, lignin and suberin deposition of exodermis cell wall. The modifications verified in leaves, such as higher density of stomata and trichome scales, showed that sucrose can induce osmotic stress in the plants. Photomixotrophic conditions, using containers with intermediate rate of gas exchange (yellow filter) and with 15–30?g L?1 sucrose, produced an improvement in the growth traits and did not induce anatomical and physiological disturbances. 相似文献
92.
Valdir Diola Giovani Greigh de Brito Eveline Teixeira Caixeta Eunize Maciel-Zambolim Ney Sussumu Sakiyama Marcelo Ehlers Loureiro 《Tree Genetics & Genomes》2011,7(6):1199-1208
Coffee leaf rust caused by the fungus Hemileia vastatrix causes considerable economic losses for coffee producers. Although agrochemical products can provide sufficient disease control,
the use of resistant cultivars is a safer alternative. This resistance may be constrained by one or a few genetic factors,
mainly those found in material originating from interspecific hybrids. In this study, the genetic analysis of an F
2
population consisting of 224 plants derived from a crossing of Híbrido de Timor UFV 427-15 (resistant) with Catuaí Amarelo
IAC 30 (susceptible) showed that a dominant gene confers the resistance of coffee to race II of H. vastatrix. From a genetic map saturated with 25 amplified fragment length polymorphism (AFLP) markers linked to the resistance gene,
we developed a high-density genetic map with six sequence-characterized amplified region (SCAR) markers delimiting a chromosomal
region of 9.45 cM and flanking the dominant gene at 0.7 and 0.9 cM. This is the first saturated and high-density genetic map
obtained from this region containing the resistance gene. The results of this study are of great importance for the introduction
of molecular markers for marker-assisted selection; they will also facilitate studies related to the cloning, structure, and
function of race-specific genes involved in the resistance of coffee trees to H. vastatrix. 相似文献
93.
High levels of extracellular ATP lead to chronic inflammatory response in melanoma patients
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94.
Subtilisin‐like proteases (or subtilases) are a very diverse family of serine peptidases present in many organisms, but mostly in plants. With a broad spectrum of biological functions, ranging from protein turnover and plant development to interactions with the environment, subtilases have been gaining increasing attention with regard to their involvement in plant defence responses against the most diverse pathogens. Over the last 5 years, the number of published studies associating plant subtilases with pathogen resistance and plant immunity has increased tremendously. In addition, the observation of subtilases and serine protease inhibitors secreted by pathogens has also gained prominence. In this review, we focus on the active participation of subtilases in the interactions established by plants with the environment, highlighting their role in plant–pathogen communication. 相似文献
95.
96.
Fábia Guimarães-Dias Anna C. Neves-Borges Alessandra J. Conforte Leonardo Giovanella-Kampmann André V. J. Ferreira Regina M. S. Amorim Magda A. Benevent Maria Eugênia Lisei de Sá Rosilene O. Mesquita Fabiana A. Rodrigues Alexandre L. Nepomuceno Eduardo Romano Marcelo E. Loureiro Maria Fátima Grossi-de-Sá Márcio Alves-Ferreira 《Plant Molecular Biology Reporter》2016,34(6):1167-1180
97.
Evolutionary history of Trachylepis skinks in the Seychelles islands: introgressive hybridization,morphological evolution and geographic structure
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Sara Rocha Anna Perera Andreia Silva David Posada D. James Harris 《Biological journal of the Linnean Society. Linnean Society of London》2016,119(1):15-36
The Seychelles is a remarkably interesting archipelago for evolutionary studies, but only recently have molecular markers been used to explore its biogeographic patterns. Here we used morphological and molecular data to examine diversity and phylogenetic relationships of two endemic skink sister‐species from this archipelago: Trachylepis sechellensis and Trachylepis wrightii. Mitochondrial DNA genealogy rendered a monophyletic T. wrightii nested within a paraphyletic T. sechellensis, whereas nuclear DNA sequences from five unlinked markers reflected the accepted taxonomy. Hybridization and massive mtDNA introgression leading to the complete replacement of the native mtDNA lineage of T. sechellensis in some of the islands were invoked to explain this result, and morphological variation also seemed to reflect this pattern of reticulation. A Mio‐Pliocene divergence between both species is suggested. Multilocus molecular data were used to uncover biogeographic patterns within the archipelago, which reflected shared patterns with other co‐distributed lizard taxa; specifically a north–south marked structure, a close relationship between populations from Fregate and the southern islands, and a detectable isolation within the southern group, between Mahé, and Silhouette and North Islands. Gene flow from these latter islands towards the northern group was also suggested. These results add to the growing body of evidence of the influence of geographic distance and sea‐level oscillations in shaping the genetic structure of Seychellois taxa and of the existence of common biogeographic patterns across the archipelago. 相似文献
98.
Pedro A. Soares Jean A. Trejaut Teresa Rito Bruno Cavadas Catherine Hill Ken Khong Eng Maru Mormina Andreia Brandão Ross M. Fraser Tse-Yi Wang Jun-Hun Loo Christopher Snell Tsang-Ming Ko António Amorim Maria Pala Vincent Macaulay David Bulbeck James F. Wilson Leonor Gusmão Luísa Pereira Stephen Oppenheimer Marie Lin Martin B. Richards 《Human genetics》2016,135(3):309-326
99.
Flávia Rosa Santoro Gilney Charll Santos Washington Soares Ferreira Júnior Leonardo da Silva Chaves Thiago Antonio Sousa Araújo Andre Luiz Borba Nascimento André Sobral Josivan Soares Silva Juliana Loureiro Almeida Campos Ulysses Paulino Albuquerque 《Evolutionary biology》2017,44(2):216-226
The malaria hypothesis, which addresses a strong selective pressure on human genes resulting from a chain of processes that originated with the practice of agriculture, is an example of an evolutionary consequence of niche construction. This scenario has led us to formulate the following questions: Are the genetic adaptations of populations with a history of contact with malaria reflected in the local medical systems? Likewise, could environmental changes (deforestation) and the incidence of malaria result in an adaptive response in these local health care systems? We collected secondary data for the entire African continent from different databases and secondary sources and measured the response of health care systems as the variation in the richness of antimalarial medicinal plants. Our results did not indicate a cause-and-effect relationship between the tested variables and the medical systems, but a subsequent analysis of variance showed an increase in the mean of medicinal plants in regions with a higher incidence of malaria prior to disease control measures. We suggest that this response had a greater impact on local medical knowledge than other variables, such as genetic frequency and deforestation. 相似文献
100.
Ji Eun Song Jing Su Ana Loureiro Madalena Martins Artur Cavaco‐Paulo Carla Silva 《Engineering in Life Science》2017,17(10):1108-1117
Bacterial cellulose (BC) was obtained by static cultivation using commercial BC gel from scoby. BC membranes (oven dried and freeze‐dried) were swelled with 8% NaOH, in the absence and in the presence of ultrasound (US), for 30, 60, and 90 min. The influence of swelling conditions on both physico‐chemical properties and molecules entrapment was evaluated. Considering the highest levels of entrapment, an optimum swelling procedure was established: 8% NaOH for 30 min at room temperature in the presence of US. Native and PEGylated laccase from Myceliophthora thermophila was immobilized on BC membranes and a different catalytic behaviour was observed after immobilization. Native laccase presented activity values similar to published reports (5–7 U/gBC) after immobilization whereas PEGylated enzymes showed much lower activity (1–2 U/gBC). BC swelled membranes are presented herein as a potential support for the preparation of immobilized enzymes for industrial applications, like phenolics polymerization. 相似文献