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151.
Mihaela Gheorghiu Andreea Olaru Aurelia Tar Cristina Polonschii Eugen Gheorghiu 《Biosensors & bioelectronics》2009,24(12):3517-3523
A new and exciting biosensing avenue based on assessment of the non-monotonous, concentration dependent effect of pore formation is discussed. A novel kinetic model is advanced to relate surface plasmon resonance (SPR) data with actual concentrations of interacting partners. Lipid modified L1 sensor chip provide the accessible platform for SPR exploration of peptide–membrane interaction, with POPC and melittin as model systems. We show that quantitative assessment of the interaction between an antimicrobial peptide and lipid modified sensors is capable to provide both sensing avenues and detailed mechanistic insights into effects of pore-forming compounds. The proposed model combined with appropriate design of the experimental protocol adds a new depth to the classic SPR investigation of peptide–lipid interaction offering a quantitative platform for detection, improved understanding of the manifold facets of the interaction and for supporting the controlled design of novel antimicrobial compounds. This biosensing approach can be applied to an entire set of pore-forming compounds including antimicrobial peptides and exo-toxins. 相似文献
152.
- The shift from shade‐intolerant species to shade‐tolerant mesophytic species in deciduous and mixed forests of the temperate zone is well described in studies from North America. This process has been termed mesophication and it has been linked to changes in fire regime. Fire suppression results in the cessation of establishment of heliophytic, fire‐dependent tree species such as oak (Quercus) and pine (Pinus). Due to the scarcity of old‐growth forests in Europe, data on long‐term compositional changes in mixed forests are very limited, as is the number of studies exploring whether fire played a role in shaping the dynamics.
- The aim of this study was to reconstruct tree succession in a 43‐ha natural mixed deciduous forest stand in Bia?owie?a Forest (BF), Poland using dendrochronological methods. In addition, the presence of aboveground fire legacies (charred and fire‐scarred deadwood) enabled the fire history reconstruction.
- Dendrochronological data revealed tree establishment (Quercus) back to the end of the 1500s and fires back to 1659. Under a regime of frequent fires until the end of the 18th century, only oak and pine regenerated, sporadically. A shift in the fire regime in the first half of the 19th century triggered oak and pine cohort regeneration, then gradually spruce (Picea) encroached. Under an increasingly dense canopy and less flammable conditions, regeneration of shade‐tolerant Carpinus, Tilia, and Acer began simultaneously with the cessation of oak and pine recruitment.
- Synthesis. The study reports the first evidence of mesophication in temperate Europe and proves that fire was involved in shaping the long‐term dynamics of mixed deciduous forest ecosystems. Our data suggest that fire exclusion promoted a gradual recruitment of fire‐sensitive, shade‐tolerant species that inhibited the regeneration of oak and pine in BF.