全文获取类型
收费全文 | 18993篇 |
免费 | 1467篇 |
国内免费 | 8篇 |
出版年
2023年 | 99篇 |
2022年 | 222篇 |
2021年 | 484篇 |
2020年 | 274篇 |
2019年 | 328篇 |
2018年 | 480篇 |
2017年 | 423篇 |
2016年 | 670篇 |
2015年 | 999篇 |
2014年 | 1098篇 |
2013年 | 1289篇 |
2012年 | 1682篇 |
2011年 | 1620篇 |
2010年 | 1018篇 |
2009年 | 760篇 |
2008年 | 1208篇 |
2007年 | 1064篇 |
2006年 | 1031篇 |
2005年 | 977篇 |
2004年 | 880篇 |
2003年 | 749篇 |
2002年 | 748篇 |
2001年 | 228篇 |
2000年 | 179篇 |
1999年 | 182篇 |
1998年 | 151篇 |
1997年 | 109篇 |
1996年 | 101篇 |
1995年 | 76篇 |
1994年 | 63篇 |
1993年 | 71篇 |
1992年 | 104篇 |
1991年 | 97篇 |
1990年 | 84篇 |
1989年 | 76篇 |
1988年 | 51篇 |
1987年 | 54篇 |
1986年 | 62篇 |
1985年 | 41篇 |
1984年 | 40篇 |
1983年 | 27篇 |
1981年 | 38篇 |
1980年 | 23篇 |
1979年 | 31篇 |
1978年 | 26篇 |
1977年 | 28篇 |
1975年 | 38篇 |
1974年 | 23篇 |
1973年 | 30篇 |
1971年 | 30篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
91.
92.
The virus origin of a Czechoslovak isolate of potato witches' broom disease is discounted: electron micrographs of ultrathin section of phloem tissues from plants infected with potato witches' broom disease demonstratedMycoplasma-like bodies, spherical or elongated showing binary fission and fragmentation. The minute corpuscles have a diameter of about 50–60 nm, the largest bodies of 1000 nm. The width of elongated filamentous structures is about 200 nm, most oval bodies have a diameter of 250 nm. A weak tetracycline treatment of diseased plants causes a delay of symptom development; a strong dose of tetracycline (applied by means of the wick method into the stem) inhibits symptom appearance completely. Tetracycline produces a phytotoxic effect inhibiting the growth of tomato plants and causing (at higher concentrations) necrosis and death of these plants. There is a note in the paper dealing with the term “mycoplasma”. The word mycoplasma in the sense ofEriksson (1897) or ofMereschkowsky (1910) does not correspond to the genus nameMycoplasma Nowak (1929). 相似文献
93.
94.
95.
A further study on the dietary-regulated biosynthesis of high-sulphur wool proteins 总被引:2,自引:2,他引:0
下载免费PDF全文
![点击此处可从《The Biochemical journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
When the diet of sheep is supplemented by the infusion of sulphur-containing amino acids or casein into the abomasum, the newly synthesized wool shows characteristic changes in its amino acid composition, with significant increases in cystine, proline and serine and decreases in aspartic acid and phenylalanine. This modification seems to be due entirely to an alteration in the overall composition of the high-sulphur proteins and to an increase in their proportion in the fibre. These variations are not the result of a change in the composition of individual proteins, but are due to alterations in their relative proportions and to the initiation of the synthesis of `new' proteins, many of which are extremely rich in cystine. It is suggested that the heterogeneity of the high-sulphur proteins may be due, in part, to similar changes in composition caused by natural variations in the nutrition of sheep. 相似文献
96.
Jaroslav Brčák 《Biologia Plantarum》1965,7(2):93-97
Chenopodium species react on infection with tobacco mosaic virus by the formation of chlorotic or necrotic lesions and later by the abscission of infected leaves. A transition of local infection into the stem has been observed exceptionally inChenopodium quinoa, C. hybridum, andC. rubrum, but no systemic infection of the leaves followed. Systemic infection was demonstrated only inC. polyspermum andC. murale. The recovery of new sprouts was demonstrated in C.murale in the late chronic phase of infection. 相似文献
97.
The human immunoglobulin heavy-chain constant region gene locus is organized in three main gene groups, the physical distances of which are unknown. Different types of gene deletions, originated by unequal crossingover, have been found to encompass one or more genes in the locus. We have analyzed some of these deletions by means of pulsed-field gel electrophoresis, which allows resolution of large DNA fragments. By identifying a fragment containing two of the main gene groups and by observing the size reduction of this fragment in subjects with deletions, we were able to estimate the distance between the two groups and better locate the pseudogene in-between. 相似文献
98.
Neural cell recognition molecule F11: homology with fibronectin type III and immunoglobulin type C domains 总被引:27,自引:0,他引:27
We report here the complete cDNA sequence of F11 130 kd polypeptide, a chick neural cell surface-associated glycoprotein implicated in neurite fasciculation and elongation. The predicted protein sequence of 1010 amino acids includes an amino-terminal signal peptide and a carboxy-terminal hydrophobic stretch, which is compatible with the consensus motif for covalent attachment of glycosyl-phosphatidylinositol. Accordingly, F11 lacks an intracellular domain, which is consistent with evidence obtained from protease protection experiments on isolated microsomes. In addition, the molecule comprises six domains related to the immunoglobulin domain type C and four resembling fibronectin repeat type III. Both types of repeats resemble those present in neural cell adhesion molecules L1 and N-CAM. The possible identity of F11 with the chick neural glycoprotein contactin is discussed. 相似文献
99.
Surface distribution of the mannose 6-phosphate receptors in epithelial Madin-Darby canine kidney cells 总被引:8,自引:0,他引:8
We have analyzed the surface polarity of both the cation-independent (CI-MPR) and the cation-dependent (CD-MPR) mannose 6-phosphate receptors in the epithelial Madin-Darby canine kidney (MDCK) cell line grown on polycarbonate filters. The surface localization was studied by plasma membrane domain-specific surface labeling methods and by confocal microscopy using MPR-specific antibodies. The CI-MPR was shown to be exclusively present on the basolateral cell surface. In contrast, the CD-MPR was expressed neither apically nor basolaterally. However, an intracellular pool of CD-MPR could be detected. In MDCKII-RCAr cells, cell surface CI-MPR was shown to recycle between the basolateral plasma membrane and the trans-Golgi network. After exogalactosylation, cell surface CI-MPR acquired sialic acid residues in a time-dependent manner. Furthermore, the basolateral CI-MPR was shown to be functional. Lysosomal enzymes, bearing the mannose 6-phosphate recognition marker, were taken up from the basolateral medium and endocytosed into the cells. Uptake of lysosomal enzymes from the apical side was insignificant and not MPR mediated. These results extend previous immunoelectron microscopic studies on the intracellular polarity of the CI-MPR (Parton, R. G., Prydz, K., Bomsel, M., Simons, K., and Griffiths, G. (1989) J. Cell Biol. 109, 3259-3272) which showed that the CI-MPR was present in basolateral early endosomes and in late endosomes but absent from apical early endosomes. 相似文献
100.
Andrea Streit reas Faissner Bernd Gehrig Melitta Schachner 《Journal of neurochemistry》1990,55(5):1494-1506
The monoclonal L5 antibody reacts with an N-glycosidically linked carbohydrate structure which is present on the neural cell adhesion molecule L1, neural chondroitin sulfate proteoglycans, and other not yet identified glycosylated proteins. Using this antibody, we isolated and characterized proteoglycans from adult mouse brain and cultured astrocytes biosynthetically labeled with Na2 35SO4 and a 3H-amino acid mixture. Our data suggest that the L5 proteoglycans of both sources are identical in their biochemical properties. The apparent molecular mass of the L5 proteoglycan is approximately 500 kDa. Digestion of the iodinated L5 proteoglycan from mouse brain and of the [35S]methionine-labeled L5 proteoglycan from cultured astrocytes with proteinase-free chondroitinases ABC and AC revealed three major core proteins with apparent molecular masses of approximately 380, 360, and 260 kDa. These represent molecularly distinct protein cores. 相似文献