全文获取类型
收费全文 | 4106篇 |
免费 | 402篇 |
出版年
2021年 | 40篇 |
2020年 | 29篇 |
2019年 | 52篇 |
2018年 | 68篇 |
2017年 | 63篇 |
2016年 | 93篇 |
2015年 | 123篇 |
2014年 | 133篇 |
2013年 | 228篇 |
2012年 | 247篇 |
2011年 | 264篇 |
2010年 | 185篇 |
2009年 | 159篇 |
2008年 | 183篇 |
2007年 | 172篇 |
2006年 | 183篇 |
2005年 | 171篇 |
2004年 | 165篇 |
2003年 | 170篇 |
2002年 | 165篇 |
2001年 | 115篇 |
2000年 | 114篇 |
1999年 | 98篇 |
1998年 | 49篇 |
1997年 | 46篇 |
1996年 | 47篇 |
1995年 | 45篇 |
1994年 | 45篇 |
1993年 | 43篇 |
1992年 | 81篇 |
1991年 | 52篇 |
1990年 | 49篇 |
1989年 | 60篇 |
1988年 | 34篇 |
1987年 | 47篇 |
1986年 | 52篇 |
1985年 | 38篇 |
1984年 | 36篇 |
1983年 | 48篇 |
1982年 | 32篇 |
1981年 | 39篇 |
1980年 | 29篇 |
1979年 | 26篇 |
1978年 | 32篇 |
1977年 | 25篇 |
1975年 | 25篇 |
1974年 | 21篇 |
1973年 | 20篇 |
1971年 | 20篇 |
1954年 | 30篇 |
排序方式: 共有4508条查询结果,搜索用时 15 毫秒
971.
Frank B. Jensen Niels A. Andersen Norbert Heisler 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1987,157(5):533-541
Summary Adult carp were subjected to 1 mM environmental nitrite for 48 h and nitrite uptake and changes in blood respiratory properties, extracellular electrolyte composition and acid-base status were examined.A constant influx of nitrite caused an accumulation of NO
2
–
in plasma to 5.4 mM in 48 h. The fraction of methaemoglobin rose with plasma [NO
2
–
] to 83%, and the arterial oxygen content decreased to extremely low values. Arterial
increased as a compensation to this O2-shortage, whereas the O2 saturation of the functional (unoxidized) haemoglobin decreased, revealing a reduction in its O2 affinity.Blood haematocrit decreased as a result of red cell shrinkage, which caused very high red cell haemoglobin (Hb) concentrations. The erythrocytic nucleoside triphosphate (NTP) concentration showed a parallel increase whereby NTP/Hb, as well as the relative contributions of ATP and GTP to NTP, remained unchanged.Plasma [Cl–] declined by 15 mM in 48 h, off-setting the plasma [NO
2
–
] increase, minor changes in plasma [HCO
3
–
] and a considerable increase in plasma [lactate]. Arterial pH and [HCO
3
–
] rose slightly during the first 24 h of nitrite exposure, but returned to control values at 48 h. The rise in plasma [lactate] was not reflected in an extracellular metabolic acidosis. Plasma [K+] increased by 94% in 48 h, revealing an uncompensated extracellular hyperkalemia, whereas plasma [Na+] decreased, and plasma [Ca++] was unchanged. Plasma osmolality remained essentially constant.The NO
2
–
accumulation could be reversed by transfer of the fish to NO
2
–
-free water, but nitrite off-loading was slower than the preceding NO
2
–
loading.Abbreviations Hb
hemoglobin
-
NTP
nucleoside triphosphate
-
Hct
hematocrit
-
fractional saturation of Hb with oxygen 相似文献
972.
H H Parving A R Andersen U M Smidt E Hommel E R Mathiesen P A Svendsen 《BMJ (Clinical research ed.)》1987,294(6585):1443-1447
The effect of long term, aggressive antihypertensive treatment on kidney function in diabetic nephropathy was studied prospectively in 11 insulin dependent diabetics (mean age 30). During the mean pretreatment period of 32 (range 23-66) months the glomerular filtration rate decreased significantly and albuminuria and the arterial blood pressure increased significantly. During the 72 (range 32-91) month period of antihypertensive treatment the average arterial blood pressure fell from 143/96 mm Hg to 129/84 mm Hg and albuminuria decreased from 1038 micrograms/min to 504 micrograms/min. The rate of decline in the glomerular filtration rate decreased from 0.89 (range 0.44-1.46) ml/min/month before treatment to 0.22 (range 0.01-0.40) ml/min/month during treatment. The rate of decline in the glomerular filtration rate was significantly smaller during the second three years compared with the first three years in patients who received long term antihypertensive treatment (greater than or equal to 6 years). One patient died from acute myocardial infarction (glomerular filtration rate 46 ml/min/1.74 m2). Effective antihypertensive treatment postpones renal insufficiency in diabetic nephropathy. 相似文献
973.
Bret A. M. Morris Kim A. Richardson Mark T. Andersen Richard C. Gardner 《Plant molecular biology》1988,11(6):795-803
Plant infections with cassava latent virus (CLV) were mediated by the Ti plasmid of Agrobacterium tumefaciens containing either monomeric or dimeric copies of the virus genome. The CLV DNAs caused typical symptoms when they were inoculated in Agrobacterium strains C58, LBA4404 and a virE mutant A1026, but not other Agrobacterium strains with mutations in other vir loci or an E. coli polA strain. Virus-specific DNA forms characteristic of normal CLV infections were found after such infection. Characterization of progeny CLV DNA from selected plants identified several infectious mutants. These were found to be small insertions and/or deletions in the coat protein gene of DNA 1 and in the intergenic region of DNA 2. 相似文献
974.
975.
H J Haga K J Andersen T Rygh B M Iversen R Matre 《The International journal of biochemistry》1988,20(8):793-800
1. Experimental proteinuria (262.9 mg protein/24 hr urine) was induced in rats by repeated intraperitoneal injections of BSA. 2. Hypertrophy of the kidney cortex was significant 8 days after the start of the BSA injections, and the activities of lysosomal enzymes in kidney cortex and urine were significantly higher in proteinuric compared to nonproteinuric rats. 3. Lysosome populations in the kidney cortex were examined by rate sedimentation of the homogenate and by rate zonal and isopycnic centrifugation of the lysosome-rich ML fraction. 4. The activity of lysosomal enzymes in the kidney cortex increased slightly, essentially in the large, fragile lysosomes mainly recovered from the proximal tubule. 5. Proteinuria induced a shift/reduction in the density of small lysosomes from 1.235 and 1.20 g/ml to 1.225 and 1.185 g/ml, respectively. 6. Proteinuria induced a new population of small lysosomes (density 1.185 g/ml) enriched in cathepsin D. 相似文献
976.
W E Niedzwiadek G T O'Bryan F A Blumenstock T M Saba T T Andersen 《Biochemistry》1988,27(18):7116-7124
Fibronectin domain structure, as influenced by interaction with heparin, calcium, or chondroitin sulfate C, was analyzed by differential scanning calorimetry. A complex thermal denaturation transition was observed with a large sharp endotherm at 63 degrees C, a broad endotherm between 70 and 80 degrees C, and an exotherm at 80-90 degrees C. Analysis of the denaturation profiles revealed the existence of four thermal transitions, 59.1, 62.2, 67.3, and 74.3 degrees C, and an exotherm at 83.9 degrees C. The calorimetric enthalpies of the four endotherms are 1146 +/- 259, 866 +/- 175, 1010 +/- 361, and 676 +/- 200 kcal/mol, respectively. In all cases, the calorimetric to van't Hoff enthalpy ratio was greater than 1.0. Computer analysis of the primary structure of fibronectin revealed 29 +/- 8% homology among the type I homology units and 28 +/- 7% homology among type III homology units, suggesting that different structural domains could arise from the same homology type. This may explain why more thermal transitions are observed for fibronectin than there are homology types. Addition of heparin to fibronectin in varying molar ratios, i.e., 10:1 to 30:1, resulted in a larger calorimetric enthalpy for the first type of structural domain (Tm = 59.1 degrees C) of fibronectin. At higher heparin to fibronectin ratios (40:1 or 75:1), the enthalpy of this domain decreased, while the others remained unchanged. In the presence of 5 mM calcium chloride, fibronectin thermal denaturation occurred at lower temperatures and was associated with precipitation of fibronectin.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
977.
978.
The function of micF RNA. micF RNA is a major factor in the thermal regulation of OmpF protein in Escherichia coli 总被引:10,自引:0,他引:10
J Andersen S A Forst K Zhao M Inouye N Delihas 《The Journal of biological chemistry》1989,264(30):17961-17970
The role of chromosomally derived micF RNA as a repressor of outer membrane protein OmpF of Escherichia coli was examined for various growth conditions. Levels of micF RNA as determined by Northern analyses are found to increase in response to cell growth at high temperature, in high osmolarity or in the presence of ethanol. After a switch to higher growth temperature, the levels of ompF mRNA and of newly synthesized OmpF decrease with time in E. coli strain, MC4100 but these decreases are not observed in isogenic micF deletion strain, SM3001. In addition, while levels of ompF mRNA are substantially reduced in both strains in response to high osmolarity or ethanol at 24 degrees C, the reduced levels in the parental strain are still 4-5-fold lower compared with the micF deletion strain. These findings indicate that chromosomally derived micF RNA plays a major role in the thermal regulation of OmpF and represses OmpF synthesis in response to several environmental signals by decreasing the levels of ompF mRNA. Analyses of the effect of a multicopy micF plasmid on the levels of OmpF and ompF mRNA after an increase in temperature indicated that multicopies of micF RNA markedly inhibited OmpF synthesis but did not accentuate ompF mRNA decrease. These data suggest that multicopy micF inhibits OmpF synthesis primarily through translational inactivation of ompF mRNA and that a limiting factor in addition to micF RNA is necessary to destabilize ompF mRNA. 相似文献
979.
Suppression of monocyte oxidative response by phenolic glycolipid I of Mycobacterium leprae 总被引:4,自引:0,他引:4
M Vachula T J Holzer B R Andersen 《Journal of immunology (Baltimore, Md. : 1950)》1989,142(5):1696-1701
Mycobacterium leprae synthesizes a unique phenolic glycolipid (PGL-I) in abundant quantities. We studied the effect of PGL-I on the generation of superoxide anion (O2-) by stimulated human monocytes. Peripheral blood monocytes pretreated with PGL-I released less O2- when stimulated with M. leprae than did control monocytes. Monocytes pretreated with dimycocerosyl phthiocerol, mycoside A of Mycobacterium kansasii, or mycoside B of Mycobacterium microti, on the other hand, released O2- in quantities comparable to control monocytes in response to M. leprae stimulation. Monocyte O2- release in response to other stimuli of the oxidative metabolic burst, such as PMA, zymosan, Mycobacterium bovis Bacille Calmette-Guérin, or M. kansasii, was unaffected by lipid pretreatment. These findings demonstrate that PGL-I has a direct effect on monocyte O2- generation in response to M. leprae and suggest that PGL-I is a modulator of phagocytic cell function. 相似文献
980.
Plasma-desorption mass spectrometry as an aid in protein sequence determination. Application of the method on a cuticular protein from the migratory locust (Locusta migratoria).
下载免费PDF全文
![点击此处可从《The Biochemical journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
The complete amino acid sequence of a structural protein, protein 8, isolated from the pharate cuticle of the locust Locusta migratoria was determined. Protein 8 contains 148 amino acid residues and has an Mr of 15,224. By the extensive use of information obtained by plasma-desorption mass spectrometry (p.d.m.s.) it was possible to reduce the need for conventional sequence determination and to improve the reliability of the results. On the basis of the determined Mr of the intact protein all the peptides that constitute the complete sequence could be isolated from a time-course enzymic digestion. The isolated peptides were sequenced by using a combination of Edman degradation and carboxypeptidase digestion monitored by p.d.m.s. The alignment of the peptides was established from the time-course digestion and further verified by a second enzymic digestion. The primary structure of the protein consists of two hydrophilic and two hydrophobic regions. The hydrophobic regions are enriched in alanine, valine and proline and dominated by a repetitive sequence Ala-Ala-Pro-(Ala/Val). The sequence strengthens the view that the cuticle proteins belong to a unique family of structural proteins. 相似文献