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21.
Anders Hay-Schmidt 《Zoomorphology》1992,112(4):189-205
Summary Planktotrophic brachiopod larvae ofGlottidia sp. have been investigated for the occurrence of glyoxylic acid induced fluorescence in catecholamines (CA), and serotonin-like (5-HT) and neuropeptide FMRFamidelike (FMRFamide) immunoreactivity (ir). The location of CA, 5-HT-ir and FMRFamide-ir cells and processes were compared with the location of neurons and nerve processes found by transmission electron microscopy. The apical ganglion contains 5-HT-ir and FMRFamideir cells and processes and CA processes. From the dorsal part of the apical ganglion extend dorsal 5-HT-ir and FMRFamide-ir processes; from the nine pairs of tentacles stage (9. pt) they project to the ventral ganglion. These dorsal lophophore processes follow themusculus lophophoralis and them. brachialis. The 5-HT-ir and some of the FMRFamide-ir processes project along the muscles to the tentacles. From the ventral part of the apical ganglion extend CA, 5-HT-ir and FMRFamide-ir processes which follow the ciliary band of the lophophore and project to the tentacles. An intense band of CA processes was also observed in the lophophore, but the dorsal/ventral location could not be ascertained. The ventral ganglion contains 5-HT-ir and FMRFamide-ir cells which project either caudally on the metasome or rostrally as part of the dorsal lophophore processes. The neuropil of the ventral ganglion contains CA, 5-HT-ir and FMRFamide-ir processes. The nervous system of the planktotrophic brachiopod larvae seems to consist of a ventral lophophore system innervating the ciliary bands and a dorsal lophophore system including the ventral ganglion innervating the body musculature. The latter system develops later in ontogeny and is regarded as a specialization due to the presence of shells and associated musculature. The former system is regarded as homologous with the nervous system of actinotroch larvae (Phoronida) and planktotrophic larvae of the echinoderms. 相似文献
22.
Effect of pyridoxine on tumor necrosis factor activitiesin vitro 总被引:1,自引:0,他引:1
Clinical trials with tumor necrosis factor (TNF) as an antitumor agent have so far given rather disappointing results. In this study we show that the naturally occuring vitamin B6 compound, pyridoxine, enhances TNF-induced cytolysis of three subclones of a mouse fibrosarcoma cell line (WEHI 164). The degree of pyridoxine-induced enhancement of TNF cytotoxicity seems to be dependent on the cells sensitivity to TNF, as the enhancement was much more pronounced in the relatively TNF resistant subclone act-R(cl.12)-WEHI 164, than in the very TNF sensitive subclone WEHI 164 clone 13. Furthermore, our study shows that pyridoxine, in contrast to its enhancing effect on TNF-induced cytotoxicity, rather inhibits TNF-induced growth of human FS-4 fibroblasts. Pyridoxine also enhances lymphotoxin (LT)-induced tumor cell killing and inhibits LT-induced fibroblast growth. Pyridoxine is a relatively non-toxic agentin vivo. Our results suggest that a combination of TNF and pyridoxine may be more efficient than TNF alone, in the treatment of cancer patients. 相似文献
23.
Cytomegalovirus (CMV) lytic-phase DNA replication requires both trans-acting factors, such as the virus-coded DNA polymerase, and a previously undefined cis-acting element, the origin, within which initiation occurs. We have located a candidate origin of CMV lytic-phase DNA replication, oriLyt, in both simian and human strains by assessing the ability of cloned restriction fragments to mediate phosphonoformic acid-sensitive DNA replication after transfection into human fibroblasts when required trans-acting factors were supplied by infection. In initial experiments the simian CMV-like strain Colburn EcoRI D fragment directed DNA replication; this fragment contains all of the single-stranded DNA-binding protein gene (dbp) and about 7 kbp of upstream sequence. A larger region upstream of human CMV dbp also mediated replication in transient assays. Subsequent subcloning and deletion analyses defined a CMV strain Colburn region sufficient for origin function, spanning about 1,300 bp in the apparently noncoding region upstream of dbp. The nucleotide sequence of this region revealed four distinct domains, containing (i) a 9-bp repeated sequence, (ii) an A+T-rich segment, (iii) an 11-bp direct repeat, and (iv) a 47-bp direct repeat. At least some part of each of these domains was required for origin function. Therefore, like the Epstein-Barr virus lytic-phase origin of DNA replication, CMV oriLyt appears to be structurally complex. 相似文献
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In the past year, additional experimental data have expanded our understanding of the molecular mechanisms that underlie nuclear receptor control of regulatory programs. It is increasingly clear -that steroid members (e.g. glucocorticoid and estrogen) and non-steroid members (e.g. retinoic acid, thyroid hormone, and vitamin D) of the nuclear receptor superfamily may utilize distinct strategies in achieving their complex control of gene regulation. 相似文献
27.
O Nilsson B W?ngberg A Wigander K Lundmark A Dahlstr?m H Ahlman A Bjartell R Ekman 《Peptides》1991,12(5):1077-1083
Delta sleep-inducing peptide (DSIP)-like immunoreactive (LI) material has been detected in nine different human pheochromocytoma tumors by immunocytochemistry. In primary tumors subjected to indirect immunofluorescence a variable number of tumor cells (25-75%) showed positive cytoplasmic labeling after incubation with DSIP antiserum. Tumor cells grown in culture were strongly labeled by the DSIP antiserum with DSIP-LI concentrated to cell bodies. Electron microscopic immunocytochemistry (immunogold labeling) of pheochromocytoma cells demonstrated DSIP-LI over the dense core of secretory granules. The presence of DSIP-LI in several HPLC fractions from conditioned culture media indicates secretion of DSIP-LI from cultured pheochromocytoma cells. The observations suggest that DSIP-LI is synthesized and stored in secretory granules before release. The different HPLC profiles from each of the tumors may reflect differences in processing or turnover of DSIP-LI in pheochromocytoma cells. 相似文献
28.
The malmö polymorphism of coagulation factor IX, an immunologic polymorphism due to dimorphism of residue 148 that is in linkage disequilibrium with two other F.IX polymorphisms
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John B. Graham Dennis B. Lubahn Susan T. Lord Julie Kirshtein Inga Marie Nilsson Anders Wallmark Rolf Ljung L. D. Frazier Jerry L. Ware Shu Wah Lin Darrell W. Stafford John Bosco 《American journal of human genetics》1988,42(4):573-580
A mouse monoclonal antibody (MAB 9.9) to coagulation factor IX (F.IX) detects a polymorphism in the plasma of normal people. Its epitope has been narrowed down to <6 amino acids in the activation peptide of the X-linked F.IX protein. The activation peptide contains a dimorphism—Thr:Ala—at position 148 of the protein. Using synthetic oligonucleotides, we have demonstrated that (1) the F.IX which reacts with 9.9 has Thr at position 148 and (2) that which does not has Ala. Positive reactors (148thr) are designated Malmö A, and negative reactors (148ala) are designated Malmö B. The plasma levels of AA women are indistinguishable from those of A men, and both B men and BB women are null against MAB 9.9. The plasma level of Malmö A in AB women is approximately half that of AA women, and “lyonization” is clearly operating in the heterozygotes. The dimorphism is in strong linkage disequilibrium with two other intragenic RFLPs, TaqI and XmnI. Furthermore, intragenic crossing-over—including double crossing-over—appears to have occurred between the three sites. Seven of the eight possible haplotypes have been identified, five in men and two others in women. The immunoassay that identifies ~50% of the AB women in the pool of Malmö A females with 95% confidence identifies men unambiguously as A or B. The assay would be very useful for population-genetic studies of the Malmö epitope if the studies were limited to men. 相似文献
29.
Anders Hargeby 《Hydrobiologia》1986,133(3):271-274
A chamber for laboratory rearing of aquatic invertebrates is described. The animals are reared in plastic dishes, through which a recirculating water volume is passed. The design ensures identical water quality in a high number of parallels. The chamber is constructed for rearing the freshwater amphipod Gammarus pulex, but can be used in studies of growth, reproduction etc. of other aquatic invertebrates as well. 相似文献
30.
Identification and characterization of a major early cytomegalovirus DNA-binding protein 总被引:9,自引:8,他引:1
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We characterized a DNA-binding protein with an approximate molecular weight of 129,000 (DB129) which is present in the nuclei of cytomegalovirus- (strain Colburn) infected cells, but not in virus particles. Results of two types of experiments demonstrated that DB129 is a member of the early class of herpesviral proteins. First, time course pulse-labeling experiments showed that its synthesis begins after that of the immediate-early protein IE94, but prior to the appearance of late viral proteins, and was reduced at late times. Second, in the presence of inhibitors of viral DNA replication, DB129 continued to be made and accumulated to elevated levels. A second set of experiments showed that DB129 bound to single-stranded DNA in vitro and was eluted by a NaCl gradient in two peaks, one at about 0.2 M and the second at about 0.6 M. A similar pattern of release was observed when infected-cell nuclei were serially extracted with increasing NaCl concentrations. In addition, treatment of nuclei with DNase I selectively released DB129, along with a small but significant fraction of another DNA-binding protein, DB51. These results suggest that DB129 is associated with DNA in vivo and that it interacts directly with single-stranded DNA. It was also shown that cells infected with human cytomegalovirus (strain Towne) contain a slightly larger counterpart to DB129, which was designated DB140. Similarities between these proteins and the major DNA-binding protein of herpes simplex virus are discussed. 相似文献