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141.
A New Klebsiella planticola Strain (Cd-1) Grows Anaerobically at High Cadmium Concentrations and Precipitates Cadmium Sulfide
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Pramod K. Sharma David L. Balkwill Anatoly Frenkel Murthy A. Vairavamurthy 《Applied microbiology》2000,66(7):3083-3087
Heavy metal resistance by bacteria is a topic of much importance to the bioremediation of contaminated soils and sediments. We report here the isolation of a highly cadmium-resistant Klebsiella planticola strain, Cd-1, from reducing salt marsh sediments. The strain grows in up to 15 mM CdCl2 under a wide range of NaCl concentrations and at acidic or neutral pH. In growth medium amended with thiosulfate, it precipitated significant amounts of cadmium sulfide (CdS), as confirmed by x-absorption spectroscopy. In comparison with various other strains tested, Cd-1 is superior for precipitating CdS in cultures containing thiosulfate. Thus, our results suggest that Cd-1 is a good candidate for the accelerated bioremediation of systems contaminated by high levels of cadmium. 相似文献
142.
Neurochemical Research - 相似文献
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144.
Anatoly V. Zaytsev Jeanne E. Mick Evgeny Maslennikov Boris Nikashin Jennifer G. DeLuca Ekaterina L. Grishchuk 《Molecular biology of the cell》2015,26(10):1829-1844
Microtubule (MT) attachment to kinetochores is vitally important for cell division, but how these interactions are controlled by phosphorylation is not well known. We used quantitative approaches in vitro combined with molecular dynamics simulations to examine phosphoregulation of the NDC80 complex, a core kinetochore component. We show that the outputs from multiple phosphorylation events on the unstructured tail of its Hec1 subunit are additively integrated to elicit gradual tuning of NDC80-MT binding both in vitro and in silico. Conformational plasticity of the Hec1 tail enables it to serve as a phosphorylation-controlled rheostat, providing a new paradigm for regulating the affinity of MT binders. We also show that cooperativity of NDC80 interactions is weak and is unaffected by NDC80 phosphorylation. This in vitro finding strongly supports our model that independent molecular binding events to MTs by individual NDC80 complexes, rather than their structured oligomers, regulate the dynamics and stability of kinetochore-MT attachments in dividing cells. 相似文献
145.
Pavel M. Borodin Ivan P. Gorlov Alexandr I. Agulnik Sergei I. Agulnik Anatoly O. Ruvinsky 《Chromosoma》1991,101(4):252-258
In order to clarify the relationship between meiotic pairing and recombination, and electron microscopic (EM) study of synaptonemal complexes (SC) and an analysis of chiasma frequency and distribution were made in male mice singly and doubly heterozygous for Robertsonian [Rb(16.17)7Bnr] and reciprocal [T(16:17)43H] translocations and also in tertiary trisomics for the proximal region of chromosome 17. In all these genotypes an extensive zone of asynapsis/desynapsis around the breakpoints was revealed. At the same time a high frequency of non-homologous pairing was observed in precentromeric regions of acrocentric chromosomes. The presence in the proximal region of chromosome 17 of the t haplotype did not affect the synaptic behaviour of this region. Chiasma frequency in the proximal region of chromosome 17 in the T(16:17)43H heterozygotes and trisomics was increased when compared with that in Robertsonian heterozygotes.by H.C. Macgregor 相似文献
146.
Igor G. Morgunov Olga V. Karpukhina Svetlana V. Kamzolova Vladimir A. Samoilenko Anatoly N. Inozemtsev 《Preparative biochemistry & biotechnology》2018,48(1):1-5
The effect of biologically active form (threo-Ds-) of isocitric acid (ICA) on oxidative stress was studied using the infusorian Paramecium caudatum stressed by hydrogen peroxide and salts of some heavy metals (Cu, Pb, Zn, and Cd). ICA at concentrations between 0.5 and 10?mM favorably influenced the infusorian cells with oxidative stress induced by the toxicants studied. The maximal antioxidant effect of ICA was observed at its concentration 10?mM irrespective of the toxicant used (either H2O2 or heavy metal ions). ICA was found to be a more active antioxidant than ascorbic acid. Biologically active pharmaceutically pure threo-Ds-ICA was produced through cultivation of the yeast Yarrowia lipolytica and isolated from the culture liquid in the form of crystalline monopotassium salt with a purity of 99.9%. 相似文献
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148.
Skalnaya Margarita G. Jaiswal Sumit K. Prakash Ranjana Prakash N. Tejo Grabeklis Andrei R. Zhegalova Irina V. Zhang Feng Guo Xiong Tinkov Alexey A. Skalny Anatoly V. 《Biological trace element research》2018,184(2):523-528
Biological Trace Element Research - The primary objective of the present study was to assess the level of selenium and toxic trace elements in wheat, rice, maize, and mustard from seleniferous... 相似文献
149.
Fang?TianEmail author Xianyong?Cao Anne?Dallmeyer Gerrit?Lohmann Xu?Zhang Jian?Ni Andrei?Andreev Patricia?M.?Anderson Anatoly?V.?Lozhkin Elena?Bezrukova Natalia?Rudaya Ulrike?Herzschuh 《Vegetation History and Archaeobotany》2018,27(2):365-379
Recent global warming is pronounced in high-latitude regions (e.g. northern Asia), and will cause the vegetation to change. Future vegetation trends (e.g. the “arctic greening”) will feed back into atmospheric circulation and the global climate system. Understanding the nature and causes of past vegetation changes is important for predicting the composition and distribution of future vegetation communities. Fossil pollen records from 468 sites in northern and eastern Asia were biomised at selected times between 40 cal ka bp and today. Biomes were also simulated using a climate-driven biome model and results from the two approaches compared in order to help understand the mechanisms behind the observed vegetation changes. The consistent biome results inferred by both approaches reveal that long-term and broad-scale vegetation patterns reflect global- to hemispheric-scale climate changes. Forest biomes increase around the beginning of the late deglaciation, become more widespread during the early and middle Holocene, and decrease in the late Holocene in fringe areas of the Asian Summer Monsoon. At the southern and southwestern margins of the taiga, forest increases in the early Holocene and shows notable species succession, which may have been caused by winter warming at ca. 7 cal ka bp. At the northeastern taiga margin (central Yakutia and northeastern Siberia), shrub expansion during the last deglaciation appears to prevent the permafrost from thawing and hinders the northward expansion of evergreen needle-leaved species until ca. 7 cal ka bp. The vegetation-climate disequilibrium during the early Holocene in the taiga-tundra transition zone suggests that projected climate warming will not cause a northward expansion of evergreen needle-leaved species. 相似文献
150.
Margarita G. Skalnaya Anatoly V. Skalny Andrey R. Grabeklis Eugeny P. Serebryansky Vasily A. Demidov Alexey A. Tinkov 《Biological trace element research》2018,186(1):12-20
The objective of the present study was to investigate the level of toxic and essential trace elements in hair of adult overweight and obese persons as well as its association with metabolic parameters. Hair trace element levels were assessed using inductively coupled plasma mass-spectrometry in 112 overweight and obese patients and 106 lean controls. Serum total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), triglycerides (TG), glucose, uric acid (UA) levels, and cholinesterase (CE) and gamma-glutamyltransferase (GGT) activity were also assessed. Excessive body weight significantly affected hair trace element levels. In particular, hair Co (33%), Cu (13%), I (30%), Mg (2-fold), Mn (25%), Zn (17%), and Ni (21%) levels were lower, whereas Al (14%) and As levels were higher in comparison to those in the control group. Correlation analysis demonstrated the most significant correlations for hair Mg with body weight, BMI, systolic and diastolic blood pressure, and UA, and for hair Al with body weight, BMI, TC, glucose, TG, CE, GGT, and UA. Multiple regression analysis demonstrated that trace elements were not associated with TC and LDL-C levels neither in crude nor in adjusted models. In turn, crude and adjusted models accounted for 25 and 43% of serum TG variance. The most significant associations were observed for hair Al, Fe, Si, and V in adjusted model. The obtained data demonstrate that obesity-related metabolic disorders may be at least partially mediated by altered trace element and mineral levels. 相似文献