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121.
本文旨在研究槲皮素填充的卵磷脂脂质体(quercetin-filled phosphatidylcholine liposomes,PCL-Q)对非致死性全身电离辐射处理的大鼠胸主动脉平滑肌细胞(smooth muscle cells,SMCs)上的大电导钙离子依赖性钾通道(large conductance Ca2+-dependent K+channels,BKCa)的作用。我们使用膜片钳技术,观察到去极化阶跃脉冲刺激的SMCs上出现外向K+电流,该电流对paxilline(BKCa的一种特异性抑制剂)敏感,因此认为该电流主体为BKCa电流。相对正常对照大鼠,辐照处理大鼠SMCs的BKCa电流幅度明显减小。这种通道抑制效应在辐照后第9天表现显著,且在整个30天的实验期间是逐渐增强的。辐照处理大鼠的SMCs的血管舒张能力可能因此而减弱。在本实验中,PCL-Q有效地恢复了辐照后SMCs的BKCa功能。而PCL-Q的组分,单一的槲皮素或空白的脂质体(PCL)恢复BKCa功能的能力较二者的组合体有明显的减弱。上述结果表明,PCL-Q能恢复辐照处理后SMCs的BKCa正常功能。PCL-Q的保护能力可能来源于其抗氧化... 相似文献
122.
James R. Skinner Trevor M. Shew Danielle M. Schwartz Anatoly Tzekov Christin M. Lepus Nada A. Abumrad Nathan E. Wolins 《The Journal of biological chemistry》2009,284(45):30941-30948
Fatty acid-induced triacylglycerol synthesis produces triacylglycerol droplets with a protein coat that includes perilipin 3/TIP47 and perilipin 4/S3-12. This study addresses the following two questions. Where do lipid droplets emerge, and how are their coat proteins recruited? We show that perilipin 3- and perilipin 4-coated lipid droplets emerge along the endoplasmic reticulum (ER). Blocking membrane trafficking with AlF4− during fatty acid-induced triacylglycerol synthesis drove perilipin 3 to the tubular ER. Forskolin, which like AlF4− activates adenylate cyclase, did not redistribute perilipin 3, but when added together with AlF4− perilipin 3 was recruited to lipid droplets rather than the ER. Thus inhibiting trafficking with AlF4− redistributed perilipin 3 differently under conditions of triacylglycerol synthesis (fatty acid addition) versus hydrolysis (forskolin) suggesting a shared acylglycerol-mediated mechanism. We tested whether diacylglycerol (DG), the immediate precursor of triacylglycerol and its first hydrolytic product, affects the distribution of perilipin 3. Stabilizing DG with the DG lipase inhibitor enhanced the perilipin 3 recruited to lipid droplets and raised DG levels in this fraction. Treating cells with a membrane-permeable DG recruited perilipin 3 to the ER. Stabilizing DG, by blocking its hydrolysis with RHC80267 or its acylation with triacsin C, enhanced recruitment of perilipin 3 to the ER. Expressing the ER enzyme DGAT1, which removes DG by converting it to triacylglycerol, attenuated perilipin 3 DG-induced ER recruitment. Membrane-permeable DG also drove perilipin 4 and 5 onto the ER. Together the data suggest that these lipid droplet proteins are recruited to DG-enriched membranes thereby linking lipid coat proteins to the metabolic state of the cell. RHC80267相似文献
123.
Anatoly Mikhailik James Mazella Linda Tseng 《Biochemical and biophysical research communications》2009,388(3):479-3612
The purpose of this study is to investigate the expression patterns and role of Notch signaling in human endometrial cells. Notch receptors, Notch 1-3 were expressed in both endometrial epithelial and stromal cells. Notch ligands, Jag1 and Dll4 and Notch target genes, Hes1 and Hey1 were predominantly expressed in endometrial epithelial cells and scarce in stromal cells. Increased de novo synthesis of Dll4 or Jag1 in stromal cells by retroviral delivery significantly induced Hes1 and Hey1. Evaluations of global gene expression by microarrays revealed that more than 400 genes in stromal cells were significantly regulated by Jag1. Gene annotation-based functional analysis classified these genes into biological processes of cell adhesion, cell structure and motility, cell communication, cell cycle, and angiogenesis. This study provides evidence that Notch ligands control the Notch gene activities and may enhance development of human endometrium. 相似文献
124.
Background
Combining multiple independent tests, when all test the same hypothesis and in the same direction, has been the subject of several approaches. Besides the inappropriate (in this case) Bonferroni procedure, the Fisher's method has been widely used, in particular in population genetics. This last method has nevertheless been challenged by the SGM (symmetry around the geometric mean) and Stouffer's Z-transformed methods that are less sensitive to asymmetry and deviations from uniformity of the distribution of the partial P-values. Performances of these different procedures were never compared on proportional data such as those currently used in population genetics. 相似文献125.
Chuvikovsky DV Esipov RS Skoblov YS Chupova LA Muravyova TI Miroshnikov AI Lapinjoki S Mikhailopulo IA 《Bioorganic & medicinal chemistry》2006,14(18):6327-6332
Ribokinase (RK) was expressed in the Escherichia coli ER2566 cells harboring the constructed expression plasmid encompassing the rbsK gene, encoding ribokinase. The recombinant enzyme was purified from sonicated cells by double chromatography to afford a preparation that was ca. 90% pure and had specific activity of 75 micromol/min mg protein. Catalytic activity of RK: (i) is strongly dependent on the presence of monovalent cations (potassium>ammonium>cesium), and (ii) is cooperatively enhanced by divalent magnesium and manganese ions. Besides D-ribose and 2-deoxy-D-ribose, RK was found to catalyze the 5-O-phosphorylation of D-arabinose, D-xylose, and D-fructose in the presence of ATP, and potassium and magnesium ions; L-ribose and L-arabinose are not substrates for the recombinant enzyme. A new radiochemical method for monitoring the formation of D-pentofuranose-5-[32P]phosphates in the presence of [gamma-32P]ATP and RK is reported. 相似文献
126.
A non-linear model for measuring grapevine leaf thickness by means of red-edge/near-infrared spectral reflectance 下载免费PDF全文
Qin J L Donald Rundquist Anatoly Gitelson Mark Steele Christopher Harkins Rebecca Briles 《农业工程》2010,30(6):297-303
Vegetation is a key element of our ecology system. The leaf area and its thickness provide valuable information about the status of our environment. Thus, there is a need for accurate, efficient, practical methodologies to estimate this biochemical parameter. Hyperspectral measurement is a means of quickly assessing leaf parameter in situ. In the past decades, there were lots of work (Boyd et al.) that focused on measurement of leaf area index, but very few on measurement of leaf thickness. In this paper, reflectance of grape leaves was measured over the spectral range of 350–1010 nm. The corresponding thickness of leaves from four grapevine cultivars was also measured as part of seventeen field campaigns undertaken during the summer of 2007. An artificial-intelligence technique, the support vector machine (SVM) model, was introduced to establish the relationship between the leaf thickness and red-edge/near-infrared (NIR) reflectance, with variability examined among individual cultivars as well as at various growth stages. The best wavelengths were variable depending on the grape cultivar and growth stage. The SVM model allows compilation of factors such as cultivar and growth stage with spectral information to yield a superior result. 相似文献
127.
128.
Antonov AS Kalinovsky AI Dmitrenok PS Kalinin VI Stonik VA Mollo E Cimino G 《Carbohydrate research》2011,346(14):2182-2192
Seven new triterpene glycosides, erylosides R1 (1), T1 (3), T2 (4), T3 (5), T4 (6), T5 (7), and T6 (8) along with the known formoside (2) were isolated from the sponge Erylus formosus collected along the Caribbean coast of Mexico. Glycoside 1 was determined as a trisaccharide, glycoside 2 as a tetrasaccharide while glycosides 3–8 were hexasaccharide. Their carbohydrate chains were unprecedented and have never been found in oligosaccharides from other biological sources, except Erylus spp. Three carbohydrate chains in the glycosides 3 and 6, 4 and 7, 5 and 8 correspondingly are new. The glycosides 1–5 have penasterol as aglycone while glycosides 6–8 proved to be glycoconjugates of 24-methylene-14-carboxy-lanost-8(9)-en-3β-ol. 相似文献
129.
Lutz Schirrmeister Guido Grosse Moritz Schnelle Margret Fuchs Matthias Krbetschek Mathias Ulrich Viktor Kunitsky Mikhail Grigoriev Andrei Andreev Frank Kienast Hanno Meyer Olga Babiy Irina Klimova Anatoly Bobrov Sebastian Wetterich Georg Schwamborn 《Palaeogeography, Palaeoclimatology, Palaeoecology》2011,299(1-2):175-196
The three main Lena Delta terraces were formed during different stages of the late Quaternary. While only the first floodplain terrace is connected with active deltaic processes, the second and third terraces, which dominate the western part of the delta, are erosional remnants of arctic paleolandscapes affected by periglacial processes. The landscape dynamics of the second and the third terraces, and their relationship to each other, are of particular importance in any effort to elucidate the late Quaternary paleoenvironment of western Beringia.Multidisciplinary studies of permafrost deposits on the second terrace were carried out at several sites of the Arga Complex, named after the largest delta island, Arga–Muora–Sise. The frozen sediments predominantly consist of fluvial sands several tens of meters thick, radiocarbon-dated from > 52 to 16 kyr BP. These sands were deposited under changing fluvial conditions in a dynamic system of shifting river channels, and have been additionally modified by synsedimentary and postsedimentary cryogenesis. Later thermokarst processes affected this late Pleistocene fluvial landscape during the Lateglacial and the Holocene. In addition, eolian activity reworked the fluvial sands on exposed surfaces at least since the Lateglacial, resulting in dune formation in some areas. Contrary to the Arga Complex, the third terrace is mainly composed of polygenetic alluvial and proluvial ice-rich permafrost sequences (Ice Complex deposits) radiocarbon-dated from 50 to 17 kyr BP which cover older fluvial sand units luminescence-dated to about 100–50 kyr BP. Paleoecological records reflect tundra-steppe conditions that varied locally, depending on landscape dynamics, during the Marine Isotope Stage (MIS) 4 and 3 periods, and a persistent change to shrub and arctic tundra during Lateglacial and Holocene periods.The study results indicate a continuous fluvial sedimentation environment for the Laptev Sea shelf in the region of the second Lena Delta terrace during the late Pleistocene, and confirm the presence of a dynamic channel system of the paleo-Lena River that flowed at the same time as the nearby subaerial Ice Complex deposits were being formed. 相似文献
130.
Imaging spectroscopy is a powerful technique for monitoring the biochemical constituents of vegetation and is critical for
understanding the fluxes of carbon and water between the land surface and the atmosphere. However, spectral observations are
subject to the sun–observer geometry and canopy structure which impose confounding effects on spectral estimates of leaf pigments.
For instance, the sun–observer geometry influences the spectral brightness measured by the sensor. Likewise, when considering
pigment distribution at the stand level scale, the pigment content observed from single view angles may not necessarily be
representative of stand-level conditions as some constituents vary as a function of the degree of leaf illumination and are
therefore not isotropic. As an alternative to mono-angle observations, multi-angular remote sensing can describe the anisotropy
of surface reflectance and yield accurate information on canopy structure. These observations can also be used to describe
the bi-directional reflectance distribution which then allows the modeling of reflectance independently of the observation
geometry. In this paper, we demonstrate a method for estimating pigment contents of chlorophyll and carotenoids continuously
over a year from tower-based, multi-angular spectro-radiometer observations. Estimates of chlorophyll and carotenoid content
were derived at two flux-tower sites in western Canada. Pigment contents derived from inversion of a CR model (PROSAIL) compared
well to those estimated using a semi-analytical approach (r
2 = 0.90 and r
2 = 0.69, P < 0.05 for both sites, respectively). Analysis of the seasonal dynamics indicated that net ecosystem productivity was strongly
related to total canopy chlorophyll content at the deciduous site (r
2 = 0.70, P < 0.001), but not at the coniferous site. Similarly, spectral estimates of photosynthetic light-use efficiency showed strong
seasonal patterns in the deciduous stand, but not in conifers. We conclude that multi-angular, spectral observations can play
a key role in explaining seasonal dynamics of fluxes of carbon and water and provide a valuable addition to flux-tower-based
networks. 相似文献