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101.
102.
Konstantin B Shumaev Andrey A Gubkin Vladimir A Serezhenkov Irina I Lobysheva Olga V Kosmachevskaya Enno K Ruuge Vadim Z Lankin Alexey F Topunov Anatoly F Vanin 《Nitric oxide》2008,18(1):37-46
Destructive effect of superoxide anions O2- derived from KO(2) or xanthine-xanthine oxidase system on dinitrosyl-iron complexes bound with bovine albumin or methemoglobin (DNIC-BSA or DNIC-MetHb) was demonstrated. The sensitivity of DNIC-BSA synthesized by the addition of DNIC with cysteine, thiosulfate or phosphate (DNIC-BSA-1, DNIC-BSA-2 or DNIC-BSA-3, respectively) to destructive action of O2- decreased in row: DNIC-BSA-1>DNIC-BSA-3>DNIC-BSA-2. The estimated rate constant for the reaction between O2- and DNIC-BSA-3 was equal to approximately 10(7)M(-1)s(-1). However, hydrogen peroxide and tert-butyl hydrogenperoxide (t-BOOH) did not induce any noticeable degradation of DNIC-BSA-3 even when used at concentrations exceeding by one order of magnitude those of the complex. As to their action on DNIC-MetHb both hydrogen peroxide and t-BOOH-induced rapid degradation of the complex. Both agents could induce the process due to the effect of alkylperoxyl or protein-derived free radicals formed at the interaction of the agents with ferri-heme groups of MetHb. Peroxynitrite (ONOO(-)) could also initiate protein-bound DNIC degradation more efficiently in the reaction with DNIC-BSA-3. Higher resistance of DNIC-MetHb to peroxynitrite was most probably due to the protective action of heme groups on ONOO(-). However, the analysis allows to suggest that the interaction of protein-bound DNICs with O2- is the only factor responsible for the degradation of the complexes in cells and tissues. 相似文献
103.
Inhibition of yes‐associated protein suppresses brain metastasis of human lung adenocarcinoma in a murine model 下载免费PDF全文
Ping‐Chih Hsu Jinbai Miao Zhen Huang Yi‐Lin Yang Zhidong Xu Joanna You Yuyuan Dai Che‐Chung Yeh Geraldine Chan Shu Liu Anatoly Urisman Cheng‐Ta Yang David M. Jablons Liang You 《Journal of cellular and molecular medicine》2018,22(6):3073-3085
Yes‐associated protein (YAP) is a main mediator of the Hippo pathway and promotes cancer development and progression in human lung cancer. We sought to determine whether inhibition of YAP suppresses metastasis of human lung adenocarcinoma in a murine model. We found that metastatic NSCLC cell lines H2030‐BrM3(K‐rasG12C mutation) and PC9‐BrM3 (EGFRΔexon19 mutation) had a significantly decreased p‐YAP(S127)/YAP ratio compared to parental H2030 (K‐rasG12C mutation) and PC9 (EGFRΔexon19 mutation) cells (P < .05). H2030‐BrM3 cells had significantly increased YAP mRNA and expression of Hippo downstream genes CTGF and CYR61 compared to parental H2030 cells (P < .05). Inhibition of YAP by short hairpin RNA (shRNA) and small interfering RNA (siRNA) significantly decreased mRNA expression in downstream genes CTGF and CYR61 in H2030‐BrM3 cells (P < .05). In addition, inhibiting YAP by YAP shRNA significantly decreased migration and invasion abilities of H2030‐BrM3 cells (P < .05). We are first to show that mice inoculated with YAP shRNA‐transfected H2030‐BrM3 cells had significantly decreased metastatic tumour burden and survived longer than control mice (P < .05). Collectively, our results suggest that YAP plays an important role in promoting lung adenocarcinoma brain metastasis and that direct inhibition of YAP by shRNA suppresses H2030‐BrM3 cell brain metastasis in a murine model. 相似文献
104.
Styliani Consta Anatoly Malevanets Myong In Oh Mahmoud Sharawy 《Molecular simulation》2018,44(13-14):1033-1043
AbstractThe free energy calculation method emerges as a viable technique for ‘in-silico’ calorimetry. Efficient sampling techniques and the good choice of a reaction path connecting the reactant and the product state enable accurate computations of the free energy differences. We argue that in many cases the thermodynamic integration technique has the lowest variance when the transformation between the reactant and the product state proceeds along the natural path of the studied chemical reaction. We provide examples of free energy calculations for the fragmentation of the charged clusters and the swapping reaction of oligomer formation in proteins that follow a tentative reaction mechanism. 相似文献
105.
Elena Berezhnaya Maria Neginskaya Anatoly B. Uzdensky Andrey Y. Abramov 《Molecular neurobiology》2018,55(1):90-95
Photodynamic therapy is selective destruction of cells stained with a photosensitizer upon irradiation with light at a specific wavelength in the presence of oxygen. Cell death upon photodynamic treatment is known to occur mainly due to free radical production and subsequent development of oxidative stress. During photodynamic therapy of brain tumors, healthy cells are also damaged; considering this, it is important to investigate the effect of the treatment on normal neurons and glia. We employed live-cell imaging technique to investigate the cellular mechanism of photodynamic action of radachlorin (200 nM) on neurons and astrocytes in primary rat cell culture. We found that the photodynamic effect of radachlorin increases production of reactive oxygen species measured by dihydroethidium and significantly decrease mitochondrial membrane potential. Mitochondrial depolarization was independent of opening of mitochondrial permeability transition pore and was insensitive to blocker of this pore cyclosporine A. However, irradiation of cells with radachlorin dramatically decreased NADH autofluorescence and also reduced mitochondrial NADH pool suggesting inhibition of mitochondrial respiration by limitation of substrate. This effect could be prevented by inhibition of poly (ADP-ribose) polymerase (PARP) with DPQ. Thus, irradiation of neurons and astrocytes in the presence of radachlorin leads to activation of PARP and decrease in NADH that leads to mitochondrial dysfunction. 相似文献
106.
Nikolay I. Zhurilo Mikhail V. Chudinov Andrey V. Matveev Olga S. Smirnova Irina D. Konstantinova Anatoly I. Miroshnikov Alexander N. Prutkov Lyubov E. Grebenkina Natalya V. Pulkova Vitaly I. Shvets 《Bioorganic & medicinal chemistry letters》2018,28(1):11-14
The novel isosteric ribavirin analogues were synthesized by two different ways. Some of them showed significant antiviral action against hepatitis C virus (HCV), herpes simplex (HCV-1) and influenza A virus comparable to that of ribavirin itself. The data obtained confirm the proposed theory of the ribavirin possible antiviral activity mechanism related with bioisosterism. 相似文献
107.
Svetlana E Medvedeva Anatoly Boyandin Yuly Lankin Dmitry Kotov Emma Rodicheva Lyudmila Popova 《Luminescence》2005,20(2):90-96
The Institute of Biophysics SB RAS hosts and maintains a specialized collection of luminous bacteria (CCIBSO 836) containing over 700 strains isolated in various regions of the world's oceans. The culture collection is a source of lux genes and biologically active substances. The wide application of bioluminescence in medicine and ecology has given importance to analysing information on the structure and functioning of bioluminescence systems in natural and transgenic microorganisms, as well as on their features that are closely interrelated with bioluminescence. The aims of our BIOLUMBASE database are: gathering information on microorganisms with lux genes, their analysis and free access, and distribution of this data throughout the global network. The database includes two sections, natural and transgenic luminous microorganisms, and is updated by our own experimental results, the published literature and internet resources. For the future, a publicly available internet site for BIOLUMBASE is planned. This will list the strains and provide comprehensive information on the properties and functions of luminous bacteria, the mechanisms of regulation of bioluminescence systems, constructs with lux genes, and applications of bioluminescence in microbiology, ecology, medicine and biotechnology. It is noteworthy that this database will also be useful for evaluation of biological hazards of transgenic strains. Users will be able to carry out bibliographic and strain searches starting from any feature of interest. 相似文献
108.
Yvonne Lundberg Giwercman Andrej Nikoshkov Kristina Lindsten Birgitta Byström Å. Pousette Alexander V. Chibalin Sivonne Arvidsson Anatoly Tiulpakov Tatiana V. Semitcheva Valentina Peterkova Kerstin Hagenfeldt E. Martin Ritzén A. Wedell 《Human genetics》1998,103(4):529-431
Five mutations in the ligand-binding domain of the androgen receptor gene were identified in patients with complete (A765T,
C784Y, R831X and M895T) or partial (R840G) androgen insensitivity. A765T and R831X have been reported previously whereas the
other three mutations are novel. Receptors carrying these mutations were transiently expressed in COS-1 cells, and androgen
binding and capacity to transactivate an androgen-responsive reporter gene were assayed. C784Y led to abolished androgen
binding and transactivating capacity, R840G and M895T showed reduced specific binding and partial transactivation. The in
vitro functions of the R840G and M895T mutants were improved with supraphysiological concentrations of steroid.
Received: 10 June 1998 / Accepted: 10 September 1998 相似文献
109.
Several anecdotal reports indicate that walruses (Odobenus rosmarus) occasionally attack seabirds, which potentially impacts local bird populations. However, the manner in which walruses interact with seabirds and the motivational basis of such interactions have not been investigated. Here, we describe and characterize the behaviour of wild Pacific walruses (O. r. divergens) directed at seabirds in water near the summer haulout in the Chukchi Sea. Although most walruses showed no interest in seabirds nearby, some individuals intentionally approached them both alone and in groups. A total of 74 seabird encounters for 71 individually identified walruses were registered. Behavioural analysis based on detailed observations, photography and video recording showed that the most common types of walrus behaviour toward a bird were approach by surfacing and splash, approach by surfacing and hit and attack from below. Immature individuals initiated 82% of encounters. The motivation to approach a bird was low in adult individuals, with the majority of encounters involving adults initiated by males. Walrus encounters with live birds showed a very low rate of bird kill. Encounters with dead birds were followed by further manipulations with bird carcasses, which included both the consumption of bird soft tissue and object play taking the form of drop–catch behaviour. In addition eight cases of the object-oriented play with a bird carcass in a group of walruses were observed. Object play in wild walruses is reported for the first time. Our results indicate that in seabird encounters, walruses display diverse behavioural patterns, not limited to predatory responses. 相似文献
110.
High‐Temperature Treatment of Li‐Rich Cathode Materials with Ammonia: Improved Capacity and Mean Voltage Stability during Cycling 下载免费PDF全文
Evan M. Erickson Hadar Sclar Florian Schipper Jing Liu Ruiyuan Tian Chandan Ghanty Larisa Burstein Nicole Leifer Judith Grinblat Michael Talianker Ji‐Yong Shin Jordan K. Lampert Boris Markovsky Anatoly I. Frenkel Doron Aurbach 《Liver Transplantation》2017,7(18)
Li‐rich electrode materials of the family x Li2MnO3·(1?x )LiNia Cob Mnc O2 (a + b + c = 1) suffer a voltage fade upon cycling that limits their utilization in commercial batteries despite their extremely high discharge capacity, ≈250 mA h g?1. Li‐rich, 0.35Li2MnO3·0.65LiNi0.35Mn0.45Co0.20O2, is exposed to NH3 at 400 °C, producing materials with improved characteristics: enhanced electrode capacity and a limited average voltage fade during 100 cycles in half cells versus Li. Three main changes caused by NH3 treatment are established. First, a general bulk reduction of Co and Mn is observed via X‐ray photoelectron spectroscopy and X‐ray absorption near edge structure. Next, a structural rearrangement lowers the coordination number of Co? O and Mn? O bonds, as well as formation of a surface spinel‐like structure. Additionally, Li+ removal from the bulk causes the formation of surface LiOH, Li2CO3, and Li2O. These structural and surface changes can enhance the voltage and capacity stability of the Li‐rich material electrodes after moderate NH3 treatment times of 1–2 h. 相似文献