No first night shift effect observed following a nocturnal main sleep and a prophylactic 1-h afternoon nap

ABSTRACT

Neurobehavioural impairment on the first night shift is often greater than on subsequent night shifts due to extended wakefulness. The aim of the study was to determine whether a 1-h afternoon nap prior to the first night shift is sufficient to produce neurobehavioural performance at levels comparable to the second night shift. Twelve male volunteers (mean age 22.9 years) participated in a laboratory protocol that simulated two 12-h night shifts. A nap preceded the first shift and a 7-h daytime sleep was scheduled between shifts. Neurobehavioural performance and subjective sleepiness measured across each night did not significantly differ between first and second shifts.     

Isolation, characterization and oxygen equilibrium of an extracellular haemoglobin from Eunice aphroditois (Passas).

The extracellular haemoglobin from the polychaeta,Eunice aphroditois, existed as a mixture of a heavy major component (so20, w = 56.96 +/- 0.125) and a light minor component (so20, w = 10.00 +/- 0.13S), the latter probably being a dissociation product of the former. The molecular weight of the purified heavier component, as detetermined by sedimentation equilibrium, was 3.44 x 10(6) +/- 0.04x10(6). The molecule had the electron-microscopic appearance typical of annelid haemoglobins, consisting of a stack of two hexagonal plates, with dimensions 26.32 +/- 0.27 nm across the flats of the hexagon, height of stack 17.86 +/- 0.34 nm. The sugar composition is reported, and the isoelectric point was approx. pH7.8. The haem content was 2.31 +/- 0.01%, corresponding to a minimal mol.wt. of 26700. Detergent/gel electrophoresis revealed the presence of at least four bands with molecular weights in the range 14600-31000. Five N-terminal amino acids were found. In addition to the 10S component, which co-existed with the 57S component at all pH values in the range 4.0-10.6, at low pH values (less than pH.5.0) A 16S and a 1.9 S component were found. The absorption and circular-dichroic spectra are reported, and the alpha-helical content, calculated from the ellipticity at 222 nm, was about 40%. The molecule bound O2 co-operatively with a maximum value of the Hill coefficient, h, of 3.9. Over the pH range 7.0-8.0 there was a positive Bohr effect.     

Molecules that inhibit T-cell functions: cytochemical localization and shuttling

Adaptive immune responses to antigens are mediated by specific receptors expressed on B cells (BCR's) and T cells (TCR's). Effector cells and memory cells are produced following a proliferative wave that accounts for clonal expansion. If not down-regulated, clonal expansion might lead to uncontrolled lymphoproliferation that would be harmful for the organism. Several mechanisms that account for the down-sizing of activated lymphocyte clones are briefly reviewed here. We next consider in detail one such mechanism that deals with the functional characterization and the immunocytochemical localization of two T-cell inhibitory molecules, namely the Cytotoxic T Lymphocyte Antigen-4 (CTLA-4) and the HP-F1 antigen, both present in all T lymphocytes. CTLA-4 and HP-F1 inhibit CD4+ T-helper cell proliferation and the lytic ability of CD8+ T-cytotoxic cells in non-specific and in antigen-specific cytolytic assays. Interestingly, a clonal distribution exists as for the ability of CTLA-4 and HP-F1 to inhibit T-cell functions. In resting and activated T cells, both molecules are largely confined in the endosomal compartment, as shown by immunofluorescence analyses. However, upon interaction of T cells with Antigen-Presenting Cells (APC's) or with target cells that must be killed, CTLA-4 molecules are transported to the plasma membrane, at the site of cell-to-cell contact where, following interaction with ligands, they trigger inhibitory signals.     

Cunninghamella elegans biomass optimisation for textile wastewater biosorption treatment: an analytical and ecotoxicological approach

The effect of pre-treatments on the composition of Cunninghamella elegans biomass and on its biosorption yields in the treatment of simulated textile wastewaters was investigated. The inactivated biomass was subjected to physical treatments, such as oven drying and lyophilisation, and chemical treatments using acid or alkali. The wastewater colour, COD and toxicity variations were evaluated. The lyophilisation sped up the biosorption process, whereas the chemical pre-treatment changed the affinity of biomass for different dyes. The alkali per-treated biomass achieved the highest COD reduction in the treatment of alkali wastewaters, probably because no release of alkali-soluble biomass components occurred under the alkaline pH conditions. Accordingly, only the acid pre-treated biomass decreased the COD of the acidic effluent. The ecotoxicity test showed significant toxicity reduction after biosorption treatments, indicating that decolourisation corresponds to an actual detoxification of the treated wastewaters. Fourier transform infrared spectroscopy, differential scanning calorimetry and thermogravimetric analyses of biomasses allowed highlighting their main chemical and physical properties and the changes induced by the different pre-treatments, as well as the effect of the chemical species adsorbed from wastewaters.     

Microbiological quality of drinking water from dispensers in Italy

Background  

Water coolers are popular in office buildings and commercial stores and the quality of this source of drinking water has the potential to cause waterborne outbreaks, especially in sensitive and immunocompromised subjects. The aim of this study was to determine the quality of water plumbed in coolers from commercial stores in comparison with tap water in Italy.     

On the oxidation of alkyl and aryl sulfides by [(Me3TACN)MnO(OH)2]: A density functional study

Density functional theory suggests that the formal 2-electron oxidation of sulfides, RR′S, to sulfoxides, by the model Mn^VO catalyst, [(TACN)Mn^V O(OH)₂]⁺, proceeds in two quite distinct 1-electron steps. Transfer of the first electron is barrierless and generates a sulfur radical cation, antiferromagnetically coupled to a Mn^IV centre via a covalent μ-oxo bridge. The second electron-transfer step is accompanied by migration of the oxygen atom to the sulfur centre, and is rate-determining. The absence of a barrier in the first step, where a sulfur radical is formed, means that the presence of electron-donating or withdrawing substituents on the sulfide has only a minor impact on the rate of reaction.     

RNA: Prebiotic Product,or Biotic Invention?

Spectacular advances in structural and molecular biology have added support to the 'RNA world' hypothesis, and provide a mandate for chemistry to explain how RNA might have been generated prebiotically on the early earth. Difficulties in achieving a prebiotically plausible synthesis of RNA, however, have led many to ponder the question posed in the title of this paper. Herein, we review recent experimental work on the assembly of potential RNA precursors, focusing on methods for stereoselective C-C bond construction by aldolisation and related processes. This chemistry is presented in the context of a broader picture of the potential constitutional self-assembly of RNA. Finally, the relative accessibility of RNA and alternative nucleic acids is considered.     

IEEE 802.11b Ad Hoc Networks: Performance Measurements

In this paper we investigate the performance of IEEE 802.11b ad hoc networks by means of an experimental study. An extensive literature, based on simulation studies, there exists on the performance of IEEE 802.11 ad hoc networks. Our analysis reveals several aspects that are usually neglected in previous simulation studies. Firstly, since different transmission rates are used for control and data frames, different transmission ranges and carrier-sensing ranges may exist at the same time in the network. In addition, the transmission ranges are in practice much shorter than usually assumed in simulation analysis, not constant but highly variable (even in the same session) and depends on several factors. Finally, the results presented in this paper indicate that for correctly understanding the behavior of an 802.11b network operating in ad hoc mode, several different ranges must be considered. In addition to the transmission range, the physical carrier sensing range is very important. The transmission range is highly dependent on the data rate and is up to 100 m, while the physical carrier sensing range is almost independent from the data rate and is approximately 200 m. Furthermore, even though stations are outside from their respective physical carrier sensing range, they may still interfere if their distance is lower than 350 m.Giuseppe Anastasi received the Laurea degree in Electronics Engineering and the Ph.D. degree in Computer Engineering both from the University of Pisa, Italy, in 1990 and 1995, respectively. He is currently an associate professor of Computer Engineering at the Department of Information Engineering of the University of Pisa. His research interests include architectures and protocols for mobile computing, energy management, QoS in mobile networks, and ad hoc networks. He was a co-editor of the book Advanced Lectures in Networking (LNCS 2497, Springer, 2002), and published more than 50 papers, both in international journals and conference proceedings, in the area of computer networking. He served in the TPC of several international conferences including IFIP Networking 2002 and IEEE PerCom 2003. He is a member of the IEEE Computer Society.Eleonora Borgia received the Laurea degree in Computer Engineering from the University of Pisa, Italy, in 2002. She is currently working toward her Ph.D. degree at the IIT Institute of the Italian National Research Council (CNR). Her research interests are in the area of the wireless and mobile networks with particular attention to MAC protocols and routing algorithms for ad hoc networks.Marco Conti received the Laurea degree in Computer Science from the University of Pisa, Italy, in 1987. In 1987 he joined the Italian National Research Council (CNR). He is currently a senior researcher at CNR-IIT. His research interests include Internet architecture and protocols, wireless networks and ad hoc networking, mobile computing, and QoS in packet switching networks. He co-authored the book Metropolitan Area Networks (Springer, London, 1997), and published in journal and conference proceedings more than 100 research papers related to design, modeling, and performance evaluation of computer-network architectures and protocols. He served as the technical program committee chair of the IFIP-TC6 conferences Networking 2002 and PWC 2003, and technical program committee co-chair of ACM WoWMoM 2002. He is serving as technical program committee co-chair of the IEEE Symposium on a World of Wireless, Mobile and Multimedia Networks (WoWMoM 2005). He served as guest editor for the Cluster Computing Journal (special issue on Mobile Ad Hoc Networking), IEEE Transactions on Computers (special issue on Quality of Service issues in Internet Web Services), and ACM/Kluwer Mobile Networks & Applications Journal (special issue on Mobile Ad hoc Networks). He is member of IFIP WGs 6.2, 6.3 and 6.8.Enrico Gregori received the Laurea in electronic engineering from the University of Pisa in 1980. He joined CNUCE, an institute of the Italian National Research Council (CNR) in 1981. He is currently a CNR research director. In 1986 he held a visiting position in the IBM research center in Zurich working on network software engineering and on heterogeneous networking. He has contributed to several national and international projects on computer networking. He has authored more than 100 papers in the area of computer networks and has published in international journals and conference proceedings and is co-author of the book Metropolitan Area Networks (Springer, London, 1997). He was the General Chair of the IFIP TC6 conferences: Networking2002 and PWC2003 (Personal Wireless Communications). He served as guest editor for the Networking2002 journal special issues on: Performance Evaluation, Cluster Computing and ACM/Kluwer Wireless Networks Journals. He is a member of the board of directors of the Create-Net association, an association with several Universities and research centres that is fostering research on networking at European level. He is on the editorial board of the Cluster Computing, of the Computer Networks and of the Wireless Networks Journals. His current research interests include: Wireless access to Internet, Wireless LANs, Quality of service in packet-switching networks, Energy saving protocols, Evolution of TCP/IP protocols.     

Decolorization and detoxication of reactive industrial dyes by immobilized fungi <Emphasis Type="Italic">Trametes pubescens</Emphasis> and <Emphasis Type="Italic">Pleurotus ostreatus</Emphasis>

Trametes pubescens and Pleurotus ostreatus, immobilized on polyurethane foam cubes in bioreactors, were used to decolorize three industrial and model dyes at concentrations of 200, 1000 and 2000 ppm. Five sequential cycles were run for each dye and fungus. The activity of laccase, Mn-dependent and independent peroxidases, lignin peroxidase, and aryl-alcohol oxidase were daily monitored during the cycles and the toxicity of media containing 1000 and 2000 ppm of each dye was assessed by the Lemna minor (duckweed) ecotoxicity test. Both fungi were able to efficiently decolorize all dyes even at the highest concentration, and the duckweed test showed a significant reduction (p 相似文献