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31.
R. Anandan B. Ganesan T. Obulesu S. Mathew K. K. Asha P. T. Lakshmanan A. A. Zynudheen 《Cell stress & chaperones》2013,18(1):121-125
Aging has been defined as the changes that occur in living organisms with the passage of time that lead to functional impairment and ultimately to death. Free radical-induced oxidative damage has long been thought to be the most important consequence of the aging process. In the present study, an attempt has been made to study the salubrious effects of dietary supplementation of chitosan on glutathione-dependent antioxidant defense system in young and aged rats. The dietary supplementation of chitosan significantly reduced the age-associated dyslipidemic abnormalities noted in the levels of total cholesterol, HDL-cholesterol, and LDL-cholesterol in plasma and heart tissue. Its administration significantly (P < 0.05) attenuated the oxidative stress in the heart tissue of aged rats through the counteraction of free radical formation by maintaining the enzymatic [glutathione peroxidase (GPx) and glutathione reductase (GR)] and non-enzymatic [reduced glutathione (GSH)] status at levels comparable to that of normal young rats. Our results conclude that dietary intake of chitosan restores the depleted myocardial antioxidant status and suggest that it could be an effective therapeutic agent in treatment of age-associated disorders where hypercholesterolemia and oxidative stress are the major causative factors. 相似文献
32.
Bhagavatheeswaran Sambhavi Ramachandran Vinu Shanmugam Sambantham Balakrishnan Anandan 《Molecular biology reports》2022,49(1):279-291
Molecular Biology Reports - Angiogenesis is the formation of new blood vessels from an existing vasculature through a series of processes such as activation, proliferation, and directed migration... 相似文献
33.
Christeller JT Farley PC Marshall RK Anandan A Wright MM Newcomb RD Laing WA 《Journal of molecular evolution》2006,63(6):747-757
The squash (Cucurbita maxima) phloem exudate-expressed aspartic proteinase inhibitor (SQAPI) is a novel aspartic acid proteinase inhibitor, constituting
a fifth family of aspartic proteinase inhibitors. However, a comparison of the SQAPI sequence to the phytocystatin (a cysteine
proteinase inhibitor) family sequences showed ∼30% identity. Modeling SQAPI onto the structure of oryzacystatin gave an excellent
fit; regions identified as proteinase binding loops in cystatin coincided with regions of SQAPI identified as hypervariable,
and tryptophan fluorescence changes were also consistent with a cystatin structure. We show that SQAPI exists as a small gene
family. Characterization of mRNA and clone walking of genomic DNA (gDNA) produced 10 different but highly homologous SQAPI
genes from Cucurbita maxima and the small family size was confirmed by Southern blotting, where evidence for at least five loci was obtained. Using primers
designed from squash sequences, PCR of gDNA showed the presence of SQAPI genes in other members of the Cucurbitaceae and in
representative members of Coriariaceae, Corynocarpaceae, and Begoniaceae. Thus, at least four of seven families of the order
Cucurbitales possess member species with SQAPI genes, covering ∼99% of the species in this order. A phylogenetic analysis
of these Cucurbitales SQAPI genes indicated not only that SQAPI was present in the Cucurbitales ancestor but also that gene
duplication has occurred during evolution of the order. Phytocystatins are widespread throughout the plant kingdom, suggesting
that SQAPI has evolved recently from a phytocystatin ancestor. This appears to be the first instance of a cystatin being recruited
as a proteinase inhibitor of another proteinase family.
[Reviewing Editor: Dr. Antony Dean] 相似文献
34.
Strengthening the laboratory diagnosis of pathogenic Corynebacterium species in the Vaccine era
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S.K. Rajamani Sekar B. Veeraraghavan S. Anandan N.K. Devanga Ragupathi L. Sangal S. Joshi 《Letters in applied microbiology》2017,65(5):354-365
Over the last three decades, successful implementation of the diphtheria vaccination in the developed and developing countries has reduced the infections caused by the toxigenic strains of Corynebacterium diphtheriae, but a concomitant increase in the invasive infections due to the nontoxigenic strains was seen. In addition, the recent reports on the emergence of nontoxigenic toxin gene‐bearing strains, having the potential to revert back to toxigenic form poses a significant threat to human beings. Besides infections caused by C. diphtheriae, the emergence of the respiratory, cutaneous and invasive infections by related pathogenic Corynebacterium species like C. ulcerans and C. pseudotuberculosis, complicate the diagnosis and management of infection. These observations together with the widespread prevalence of diphtheria in the vaccine era, necessitates the strengthening of the epidemiological surveillance and laboratory diagnosis of the pathogen. This review provides the overview of the advantages and limitations of different molecular methods and the role of MALDI‐TOF in the laboratory diagnosis of Diphtheria. The contribution of next generation sequencing technology and different genotyping techniques in understanding the pathogenicity, transmission dynamics and epidemiology of the C. diphtheriae is discussed. 相似文献
35.
Genetic characterization of a signal transduction pathway requires the isolation of mutations in the pathway. Characterization of these mutated genes and their loci enumerates the components of the pathway and leads to an understanding of the role of each gene locus in the pathway under study. We have designed and developed a strategy based on resistance to the chemical flucytosine for the identification of mutations in a given pathway. In this study, the Escherichia coli codA gene, which encodes the enzyme cytosine deaminase, was fused to the light-intensity-regulated gene promoter psbDII. Cytosine deaminase converts 5′-fluorocytosine to the toxic product 5-fluorouracil. Wild-type cells containing an intact signal transduction pathway that regulates the psbDII promoter will die in the presence of this chemical. Cells that carry mutations in the pathway that inactivate the psbDII promoter will not express the codA gene and, consequently, will live on 5′-fluorocytosine, allowing the isolation and subsequent characterization of mutations in this signaling pathway. Utilizing this selection method, we have successfully isolated and characterized mutations in the psbDII pathway. This selection scheme can be used with a tissue-specific or phase-specific promoter fused to the codA gene to direct the timing of expression of codA to obtain mutants defective in temporal or cell-specific expression of a particular pathway. This scheme also allows the isolation of mutants even when a clearly identifiable phenotype is not available. The selection scheme presented here extends the molecular tools available for the genetic dissection of signal transduction pathways. 相似文献
36.
Bimal P. Mohanty T. V. Sankar Satabdi Ganguly Arabinda Mahanty R. Anandan Kajal Chakraborty B. N. Paul Debajit Sarma J. Syama Dayal Suseela Mathew K. K. Asha Tandrima Mitra D. Karunakaran Soumen Chanda Neetu Shahi Puspita Das Partha Das Md Shahbaz Akhtar P. Vijayagopal N. Sridhar 《Biological trace element research》2016,174(2):448-458
37.
K.?K.?AshaEmail authorView authors OrcID profile K.?R.?Remya Kumari K.?Ashok Kumar Niladri?S.?Chatterjee R.?Anandan Suseela?Mathew 《International journal of peptide research and therapeutics》2016,22(3):421-433
Soft tissue from cultured farm fresh oysters (Crassostrea madrasensis) was subjected to two standard enzymatic peptide extraction procedures using pepsin and papain. The crude extracts obtained were partially purified by column chromatography and were freeze-dried. The hydrolysates obtained were compared with respect to their degree of hydrolysis (DH), antioxidant potential (AP) and total phenolic content (TPC). The hydrolysate showing better antioxidant property was further subjected to purification by high performance liquid chromatography and characterized by LC-MS/MS. Papain-digested oyster protein (OPHpap) hydrolysate showed higher DH, AP and TPC. OPHpap was further subjected to ultrafiltration and fractionated into 3 sizes namely, above 10, 3–10 and 1–3 kDa according to the molecular size. Antioxidant capacity of <3 kDa fraction OPHpap-3 evaluated by DPPH free radical scavenging assay, metal chelating activity, linoleic acid autoxidation assay showed maximum effectiveness. Of the seven fractions collected by purification of OPH-pap-3 on semi-preparative RP-HPLC, fraction 7 that showed the highest antioxidant activity was further characterized by LC-ESI-MS/MS and its sequence determined. An antioxidant peptide molecule with thirteen amino acids was identified in oyster protein hydrolysate obtained by papain digestion that may find application as a nutraceutical or may be utilized in food industry for prevention of rancidity in foods. 相似文献
38.
B. Ganesan S. Buddhan R. Anandan R. Sivakumar R. AnbinEzhilan 《Molecular biology reports》2010,37(3):1319-1327
We investigated the antioxidant preventive effect of betaine on isoprenaline-induced myocardial infarction in male albino
rats. Isoprenaline induced myocardial infarction was manifested by a moderate elevation in the levels of diagnostic marker
enzymes (alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase and creatine phosphokinase) and homocysteine
in plasma of experimental rats. Significant rise in the level of lipid peroxidation with a concomitant decline in the levels
of myocardial non-enzymic (reduced glutathione) and enzymic antioxidants (glutathione peroxidase, glutathione-S-transferase, catalase and superoxide dismutase) was also observed. Oral pretreatment with betaine significantly prevented
isoprenaline-induced alterations in the levels of diagnostic marker enzymes and homocysteine in plasma of experimental groups
of rats. It counteracted the isoprenaline-induced lipid peroxidation and maintained the myocardial antioxidant defense system
at near normal. Histopathological observations also confirmed the protective effect of betaine against isoprenaline-induced
myocardial infarction. The results of the present investigation indicate that the protective effect of betaine is probably
related to its ability to strengthen the myocardial membrane by its membrane stabilizing action or to a counteraction of free
radicals by its antioxidant property. 相似文献
39.
Fabrication of metallic nanopillar array structures and their application as electrodes in electrochemical-based biosensors are discussed in this report. Vertically standing nanopillar array structures were fabricated using an electrodeposition technique and their electrochemical characteristics were evaluated. For possible use in biosensing applications, these standing nanopillars should have sufficient mechanical stability to sustain the capillary forces caused by the nanopillar-liquid interactions in aqueous environment and should provide increased signal response in an electrochemical process. Our results showed that the developed nanopillar arrays were mechanically stable in aqueous environments and the nanostructured electrodes exhibited increased electrochemical response compared with flat electrodes. 相似文献
40.
Sampath-Kumar Anandan Richard D. Gless 《Bioorganic & medicinal chemistry letters》2010,20(9):2740-2744
The impact of various secondary and tertiary pharmacophores on in vitro potency of soluble epoxide hydrolase (sEH) inhibitors based on the unsymmetrical urea scaffold 1 is discussed. N,N′-Diaryl urea inhibitors of soluble epoxide hydrolase exhibit subtle variations in inhibitory potency depending on the secondary pharmacophore but tolerate considerable structural variation in the second linker/tertiary pharmacophore fragment. 相似文献