Aberrant antigenic expression in extranodal NK/T-cell lymphoma: a multi-parameter study from Thailand

Background

Extraosseous plasmacytoma, so called extramedullary plasmacytoma (EMP) is relatively rare in China. The aim was investigate the clinicopathologic features of EMP and the role of Immunophenotype and genotype detection in diagnosis of EMP.

Methods

Thirty-two cases of EMP were investigated retrospectively by histopathology, immunophenotype, genotype and survival analysis.

Results

Clinically, the mean age of the patients was 53.4. Most of the patients received no treatment after the diagnosis was established, and the prognosis was relatively poor. Histologically, in 40% of the cases, the neoplastic cells were grade II or III. The neoplastic cells expressed one or more PC associated antigens. The immunophenotype of EMP and inflammation of sinonasal regions with numerous PC infiltrations were compared and showed some difference in expression of CD45, CD27, CD44v6 and Bcl-2 as well. Ig light chain restriction was detected in 87.5% of the cases.

Conclusions

we described 32 Chinese cases of EMP, compare with that reported in the literature, some differences are presented, including higher percentage of grade II and III cases, clinically inconsistent treatment and management as well as poor outcome of the disease.     

Distribution and metabolism of selenite and selenomethionine in the Japanese quail

Compared to the many studies on the physiological and toxicological effects of selenium (Se) in mammals, avian Se metabolism is still an unexplored topic. Some birds are useful as poultry for human nutrition. Moreover, birds belong to higher trophic levels in the biosphere and thus may play an important role in Se circulation in the ecosystem in the same way as mammals do. In this study, we analyzed the distribution and metabolism of Se in an experimental bird, the Japanese quail, which was fed drinking water containing sodium selenite or selenomethionine (SeMet). The highest concentration of Se was detected in the pancreas, followed by down feathers, liver, and kidneys. SeMet was more efficiently incorporated into the quail than selenite. The specific and preferable distribution of Se to the high molecular weight fraction in the serum of the quail was observed only in the SeMet-ingestion group. As in mammals, selenosugar and trimethylselenonium were the major metabolites in quail excreta. Three unknown Se metabolites were detected by HPLC-ICP-MS. Although part of the metabolic pathway of Se in the Japanese quail fed selenite and SeMet was the same as that observed in mammals, the bird also showed certain avian-specific metabolic process for Se.     

Structural characterization of lipidic systems under nonequilibrium conditions

This review covers recent studies on the characterization of the dynamics of lipidic nanostructures formed via self-assembly processes. The focus is placed on two main topics: First, an overview of advanced experimental small-angle X-ray scattering (SAXS) setups combined with various sample manipulation techniques including, for instance, stop-flow mixing or rapid temperature-jump perturbation is given. Second, our recent synchrotron SAXS findings on the dynamic structural response of gold nanoparticle-loaded vesicles upon exposure to an ultraviolet light source, the impact of rapidly mixing negatively charged vesicles with calcium ions, and in?situ hydration-induced formation of inverted-type liquid-crystalline phases loaded with the local anesthetic bupivacaine are summarized. These in?situ time-resolved experiments allow real-time monitoring of the dynamics of the structural changes and the possible formation of intermediate states in the millisecond to second range. The need for investigating self-assembled systems, mainly stimuli-responsive drug nanocarriers, under nonequilibrium conditions is discussed. For pharmaceutically relevant applications, it is essential to combine these investigations with appropriate in?vitro and in?vivo studies.     

人乳汁中microRNA的稳定性研究

为了研究人乳汁中的microRNAs(miRNAs)在体外储存状态下的稳定性。本研究采用模拟体外乳汁储存条件,对乳汁进行室温孵育24h,反复冻融6次,100℃孵育10min,以及模拟体内消化环境(RNA酶A/T137℃孵育1h)的处理条件,通过实时荧光定量PCR(qRT-PCR)方法检测处理前后乳汁中9个内源性免疫相关和1个外源性人工合成miRNA的相对表达量变化差异。研究结果显示:处理前后乳汁中内源性miRNAs和外源性miRNA均显著性降解(p<0.01)。内源性miRNAs降解为初始量的25%～70%之间,而外源性miRNA则急剧降解至初始量的0.06%～0.96%。我们的实验表明人乳汁中内源性的miRNAs较外源性miRNAs在体外不同环境下具有更强的稳定性。     

西鄂尔多斯4种荒漠植物光合作用特征与差异性

植物对光能的高效吸收、传递和转换机理是光合作用的核心。为了厘清西鄂尔多斯地区4种荒漠植物光合生理生态适应性和生境适宜性,运用LI-6800光合作用测定系统对四合木(Tetraena mongolica)、霸王(Sarcozygium xanthoxylon)、沙冬青(Ammopiptanthus mongolicus)和白刺(Nitraria tangutorum)进行光合作用日变化进行了测定。研究结果表明:(1) 4种荒漠植物的光合作用日变化的净光合速率(Pn)、蒸腾速率(Tr)、气孔导度(Gs)和胞间CO_2浓度(Ci)均表现出明显的分异;(2)净光合速率均呈"双峰"曲线的变化趋势,4种荒漠植物的净光合速率(Pn)依次排序为四合木(4.37μmol m~(-2) s~(-1))霸王(3.58μmol m~(-2) s~(-1))沙冬青(2.63μmol m~(-2) s~(-1))和白刺(2.26μmol m~(-2) s~(-1))。说明四合木和近缘种霸王比其他二种荒漠植物具有较强的光合生理生态适应性与生境适宜性;(3)从影响光合作用的有关生理生态因子来看,净光合速率与气孔导度与蒸腾速率、水分利用效率呈现明显的正相关性,与微气象因子大气相对湿度(RH)的相关性不明显;(4)水分因子是限制4种荒漠植物生长的重要因素之一。该研究以期为我国西北荒漠区珍稀濒危植物的保护提供案例借鉴与理论依据。     

Intranuclear dynamics of chromosome 6 in nurse cells of <Emphasis Type="Italic">Calliphora erythrocephala</Emphasis> Mg. (Diptera: Calliphoridae)

Intranuclear dynamics of chromosome 6 in nurse cell nuclei of Calliphora erythrocephala Mg. (Diptera: Calliphoridae) was studied. The 3D FISH method was used for the first time to study chromosome territories in highly polyploid nuclei whose chromosomes undergo morphological changes. A considerable change in the intranuclear location of chromosome 6 and a morphological alteration of the chromosome territory in the course of chromatin polytenization were revealed.     

2个猪品种的背最长肌组织中兰尼定受体(RYR1)基因表达的发育性变化

本研究采用实时荧光定量PCR方法检测了长白猪和梅山猪背最长肌组织中RYR1基因mRNA表达量在初生(0月龄)、1月龄、2月龄、3月龄、4月龄和5月龄间的变化及其与体重、肌纤维面积(CSA)和肌内脂肪含量(IMF)之间的相关性。结果表明:长白猪的体重、CSA在多数月龄均高于梅山猪,而梅山猪的IMF在多数月龄均高于长白猪。两品种RYR1基因表达量的发育性表达模式极为相似,均表现为波动起伏中逐渐上升的趋势。相同月龄时,梅山猪RYR1基因的表达量均高于长白猪。本文初步揭示了两猪种生长发育过程中RYR1基因表达的发育性变化模式和品种差异,为进一步研究RYR1基因对猪肉质性状的影响提供了基础数据。     

Comparison of selenohomolanthionine and selenomethionine in terms of selenium distribution and toxicity in rats by bolus administration

The distribution and metabolism of selenohomolanthionine (4,4'-selenobis[2-aminobutanoic acid], SeHLan), a newly identified selenoamino acid in selenized Japanese pungent radish, were compared with those of selenomethionine (SeMet) in rats. Either selenoamino acid was injected intravenously at a bolus dose of 1.0 mg Se/kg body weight. SeMet was preferably accumulated in the pancreas, increasing the serum amylase level, an index of pancreatic damage. SeHLan was preferably accumulated in the kidneys, raising the serum creatinine level, an index of kidney damage. On the other hand, the levels of two major urinary selenometabolites, i.e., Se-methylseleno-N-acetyl-galactosamine and trimethylselenonium, were comparable between SeHLan- and SeMet-administered rats, suggesting that there may be no differences in the efficiency of metabolism of these two selenoamino acids to the urinary selenometabolites despite the difference in distribution. SeHLan is expected to be a potential supplemental source of Se without inducing the onset of pancreatic damage. The specific toxicity of SeHLan to the kidneys may be avoided if its dose is lower than the one used in the present study.     

Development of ovarioles and nurse-cell cytoskeleton in <Emphasis Type="Italic">Calliphora erythrocephala</Emphasis> Mg (Diptera: Calliphoridae)

We have examined ovariole morphology and their development in Calliphora erythrocephala Mg. (Diptera: Calliphoridae). It has been shown that several regions can be distinguished in C. erythrocephala germarium. In these regions, cyst morphogenesis subsequently continues until the separation of the formed egg chamber. The germarium has variously branching fusomes. Two egg chambers develop in the C. erythrocephala vitellarium. The distribution of actin filaments and the formation of ring canals was studied in C. erythrocephala nurse cells. The diameter of the ring canal increases in the germarium zone from 1.5 μm to 14.7 μm during the third and fourth studies of oogenesis. A scheme is presented that illustrates the irregular stretching of actin rollers, which are formed during middle and late oogenesis around ring canals, which joins the proximal and neighboring parts and dramatically increases the size of nurse cells.