Untangling inflorescences in Miconieae (Melastomataceae): development,typology, and the systematic and evolutionary implications

Journal of Plant Research - This study aimed to describe the origin, position, development and typology of inflorescences in Miconieae through ontogenetic and morphological analyses using light...     

Phylloseptins: a novel class of anti-bacterial and anti-protozoan peptides from the Phyllomedusa genus

Six novel peptides called phylloseptins (PS-1, -2, -3, -4, -5, and -6) showing anti-bacterial (PS-1) and anti-protozoan (PS-4 and -5) activities were isolated from the skin secretion of the Brazilian tree-frogs, Phyllomedusa hypochondrialis and Phyllomedusa oreades. Phylloseptins have a primary structure consisting of 19-21 amino acid residues (1.7-2.1 kDa). They have common structural features, such as a highly conserved N-terminal region and C-terminal amidation. Phylloseptin-1 (FLSLIPHAINAVSAIAKHN-NH2) demonstrated a strong effect against gram-positive and gram-negative bacteria (MICs ranging from 3 to 7.9 microM), without showing significant hemolytic activity (<0.6% at the MIC range) towards mammalian cells. Atomic force microscopy experiments indicated that the bacteriolytic properties of these peptides might be related to their disruptive action on the cell membrane, characterized by a number of bubble-like formations, preceding every cell lysis. PS-4 and PS-5 showed anti-protozoan activity with IC50 at about 5 microM for Trypanosoma cruzi.     

Evaluation of the fatty acid profiles of two fairy shrimp species,Branchipus pasai Cottarelli, 1969 and Chirocephalus kerkyrensis Pesta, 1936 (Crustacea,Anostraca) fed different diets

Laboratory cultured fairy shrimps Branchipus pasai and Chirocephalus kerkyrensis, fed on an alga (Selenastrum capricornutum), a yeast (Saccharomyces cerevisiae) and an HUFA enriched yeast (Lansy PZ, produced by Artemia Systems, Gent, Belgium) were evaluated for their fatty acid (FA) profiles and total lipid content in order to obtain information on species differences in food conversion. The results indicate significant qualitative and quantitative differences (P < 0.001) in FA profiles both of feed and of fairy shrimp species. Among the three different diets, an appreciable lipid amount was recorded in the alga, as compared with baker‘s yeast which showed the poorest lipid content. The algal fatty acid profile showed adequate amounts of the EFA 18:2n6, 18:3n3 and20:5n3 (the most meaningful for aquacultural purposes). The enriched yeast was characterised by a considerable total lipid amount and by the presence of all the EFA. The overall amounts of fatty acids in the fairy shrimps correlated well with their levels in the feed(r > 0.9, P <0.001). An exception was noted in then-3 HUFA (highly unsaturated fatty acids) and n-6acids, among the individuals fed on yeast. Highly significant differences (P < 0.001) were also noted between the two fairy shrimp species fed on the same food type. When fed enriched yeast, both B. pasai and C. kerkyrensis profiles roughly reflected diet composition. However, when fed on algae or baker‘s yeast, the two species, though to different extents, exhibited higher EFA levels than those recorded in the food. This seems to support the existence of a partial FA bioconversion capacity in fairy shrimps previously noted in the case of the brine shrimp Artemia. This revised version was published online in August 2006 with corrections to the Cover Date.     

A 1.5-Mb deletion in 17p11.2-p12 is frequently observed in Italian families with hereditary neuropathy with liability to pressure palsies.

Hereditary neuropathy with liability to pressure palsies (HNPP) is an autosomal dominant disorder characterized by recurrent mononeuropathies. A 1.5-Mb deletion in chromosome 17p11.2-p12 has been associated with HNPP. Duplication of the same 1.5-Mb region is known to be associated with Charcot-Marie-Tooth disease type 1 (CMT1A), a more severe peripheral neuropathy characterized by symmetrically slowed nerve conduction velocity (NCV). The CMT1A duplication and HNPP deletion appear to be the reciprocal products of a recombination event involving a repeat element (CMT1A-REP) that flanks the 1.5-Mb region involved in the duplication/deletion. Patients from nine unrelated Italian families who were diagnosed with HNPP on the basis of clinical, electrophysiological, and histological evaluations were analyzed by molecular methods for DNA deletion on chromosome 17p. In all nine families, Southern analysis using a CMT1A-REP probe detected a reduced hybridization signal of a 6.0-kb EcoRI fragment mapping within the distal CMT1A-REP, indicating deletion of one copy of CMT1A-REP in these HNPP patients. Families were also typed with a polymorphic (CA)n repeat and with RFLPs corresponding to loci D17S122, D17S125, and D17S61, which all map within the deleted region. Lack of allelic transmission from affected parent to affected offspring was observed in four informative families, providing an independent indication for deletion. Furthermore, pulsed-field gel electrophoresis analysis of SacII-digested genomic DNA detected junction fragments specific to the 1.5-Mb HNPP deletion in seven of nine Italian families included in this study. These findings suggest that a 1.5-Mb deletion on 17p11.2-p12 is the most common mutation associated with HNPP.     

Use of the cytocentrifuge for electron microscopy investigations

A simple method of cytocentrifugally processing cell suspensions for conventional and histochemical investigations at the ultrastructural level is described. Fixed sediments from cell-poor suspensions are resuspended in an albumin-buffered solution. A few drops of the albumin cell solution are cytocentrifuged, leaving a cell disc on a plastic support. A brief dipping in a paraformaldehyde-glutaraldehyde mixture transforms the cell disc into a compact thin fragment attached to the plastic support. Cytocentrifugation of cell-rich suspensions, on the other hand, produces a thicker cell disc, which can be easily detached from the plastic slide. In both cases, the postfixation, dehydration and infiltration are directly carried out on the cell disc. The present method is particularly useful for the ultrastructural study of cell-poor suspensions and can also be performed on cell suspensions previously stained with several histochemical procedures.