全文获取类型
收费全文 | 41265篇 |
免费 | 3070篇 |
国内免费 | 6篇 |
出版年
2023年 | 196篇 |
2022年 | 272篇 |
2021年 | 644篇 |
2020年 | 478篇 |
2019年 | 518篇 |
2018年 | 1283篇 |
2017年 | 1077篇 |
2016年 | 1288篇 |
2015年 | 1869篇 |
2014年 | 1852篇 |
2013年 | 2526篇 |
2012年 | 2989篇 |
2011年 | 2825篇 |
2010年 | 1780篇 |
2009年 | 1531篇 |
2008年 | 2288篇 |
2007年 | 2233篇 |
2006年 | 2195篇 |
2005年 | 1919篇 |
2004年 | 1885篇 |
2003年 | 1700篇 |
2002年 | 1586篇 |
2001年 | 849篇 |
2000年 | 789篇 |
1999年 | 717篇 |
1998年 | 415篇 |
1997年 | 316篇 |
1996年 | 305篇 |
1995年 | 302篇 |
1994年 | 239篇 |
1993年 | 223篇 |
1992年 | 412篇 |
1991年 | 413篇 |
1990年 | 343篇 |
1989年 | 310篇 |
1988年 | 299篇 |
1987年 | 257篇 |
1986年 | 250篇 |
1985年 | 248篇 |
1984年 | 196篇 |
1983年 | 154篇 |
1982年 | 138篇 |
1981年 | 170篇 |
1980年 | 138篇 |
1979年 | 186篇 |
1978年 | 158篇 |
1977年 | 134篇 |
1976年 | 139篇 |
1975年 | 128篇 |
1974年 | 130篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
81.
82.
M. Foulon M. Robbe-Saule J. Manry L. Esnault Y. Boucaud A. Alcaïs M. Malloci M. Fanton dAndon T. Beauvais N. Labarriere P. Jeannin L. Abel J. P. Saint-Andr A. Crou Y. Delneste I. G. Boneca L. Marsollier E. Marion 《PLoS pathogens》2020,16(12)
Mycolactone, a lipid-like toxin, is the major virulence factor of Mycobacterium ulcerans, the etiological agent of Buruli ulcer. Its involvement in lesion development has been widely described in early stages of the disease, through its cytotoxic and immunosuppressive activities, but less is known about later stages. Here, we revisit the role of mycolactone in disease outcome and provide the first demonstration of the pro-inflammatory potential of this toxin. We found that the mycolactone-containing mycobacterial extracellular vesicles produced by M. ulcerans induced the production of IL-1β, a potent pro-inflammatory cytokine, in a TLR2-dependent manner, targeting NLRP3/1 inflammasomes. We show our data to be relevant in a physiological context. The in vivo injection of these mycolactone-containing vesicles induced a strong local inflammatory response and tissue damage, which were prevented by corticosteroids. Finally, several soluble pro-inflammatory factors, including IL-1β, were detected in infected tissues from mice and Buruli ulcer patients. Our results revisit Buruli ulcer pathophysiology by providing new insight, thus paving the way for the development of new therapeutic strategies taking the pro-inflammatory potential of mycolactone into account. 相似文献
83.
84.
85.
86.
87.
88.
89.
P Navarro-Rosinés C Roux A Bellemère 《Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie》2001,324(5):443-451
An ultrastructural study of Weddellomyces epicallopisma (ascomata wall, asci, ascospores and vegetative hyphae), the first done on the family Dacampiaceae, confirms most of the observations made in light microscopy. Moreover it shows that ascospores are provided with an endospore (not visible in light microscope) and that the structure of the ascospore septum is more complex. The similarity of the wall structure between the ascospore and the hyphoid appendages, developed on the upper part of the ascoma, is emphasized. 相似文献
90.
A heterozygous mutant for the two- and six-rowed character was isolated in the barley cultivar Igri through application of sodium azide to isolated microspore cultures and posterior regeneration. Six-rowed and two-rowed homozygotic plants were subsequently identified in the self-pollinated M2 progenies of the original heterozygous M1. Detailed molecular markers confirmed the isogenic nature of this recovered mutant and the original cultivar Igri. A comparative study of the anther culture response of this six-rowed induced mutant vs. diploid 'Igri' was performed to assess whether the two- or six-rowed gene influences anther culture response in barley through a pleiotropic effect or via linkage disequilibrium. No significant differences for any of the recorded variables throughout the in vitro regeneration process were detected between the 'Igri' six-rowed mutant and any of their two-rowed isogenic lines. This suggests that row-type association with anther culture response in barley cultivars is due to the effect of a tight linkage with other genes directly responsible for androgenic response. 相似文献