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71.
Wang Fubiao Sun Huimin Rong Lingling Li Zhaowei An Ting Hu Wenhai Ye Zipiao 《Plant Growth Regulation》2021,95(1):121-136
Plant Growth Regulation - To clarify the genotypic-dependent alternation in D1 protein turnover and PSII repair cycle and its relation to light intensity in senescent leaves of rice, two rice... 相似文献
72.
Kim Jung-Il Karagozlu Mustafa Zafer An Hyung-Eun Choi Tae-June Yeo Yonggu Kim Chang-Bae 《Molecular biology reports》2021,48(7):5787-5793
Molecular Biology Reports - Trichoglossus haematodus is the most popular parrots globally and one of the most bred species in Korea's zoos. However, despite its popularity, there are limited... 相似文献
73.
Wang Guang-Long An Ya-Hong Wang Ya-Hui Liu Jie-Xia Wang Ji-Zhong Sun Min Xiong Ai-Sheng 《Journal of Plant Growth Regulation》2021,40(2):787-797
Journal of Plant Growth Regulation - Gibberellins (GAs) are a group of plant hormones that play important roles in various processes. Previous studies demonstrated that GA can increase the... 相似文献
74.
Alexandru Paraschiv Thibaut J. Lagny Christian Vanhille Campos Evelyne Coudrier Patricia Bassereau Anđela Šarić 《Biophysical journal》2021,120(4):598-606
The cell membrane is an inhomogeneous system composed of phospholipids, sterols, carbohydrates, and proteins that can be directly attached to underlying cytoskeleton. The protein linkers between the membrane and the cytoskeleton are believed to have a profound effect on the mechanical properties of the cell membrane and its ability to reshape. Here, we investigate the role of membrane-cortex linkers on the extrusion of membrane tubes using computer simulations and experiments. In simulations, we find that the force for tube extrusion has a nonlinear dependence on the density of membrane-cortex attachments: at a range of low and intermediate linker densities, the force is not significantly influenced by the presence of the membrane-cortex attachments and resembles that of the bare membrane. For large concentrations of linkers, however, the force substantially increases compared with the bare membrane. In both cases, the linkers provided membrane tubes with increased stability against coalescence. We then pulled tubes from HEK cells using optical tweezers for varying expression levels of the membrane-cortex attachment protein Ezrin. In line with simulations, we observed that overexpression of Ezrin led to an increased extrusion force, while Ezrin depletion had a negligible effect on the force. Our results shed light on the importance of local protein rearrangements for membrane reshaping at nanoscopic scales. 相似文献
75.
76.
Duan Yali Li Changchong Deng Li An Shuhua Zhu Yun Wang Wei Zhang Meng Xu Lili Xu Baoping Chen Xiangpeng Xie Zhengde 《中国病毒学》2021,36(3):382-392
To investigate the molecular epidemiology and genetic variation of human adenovirus type 7(HAdV-7) in children with acute respiratory infections(ARI) in China. HAdV-7-positive respiratory samples collected from children with ARI in Beijing, Shijiazhuang, Wenzhou and Guangzhou from 2014–2018 were selected for gene amplification and sequence analysis. Fifty-seven HAdV-7 clinical strains with hexon, penton base and fiber gene sequences were obtained. Meanwhile17 strains were selected randomly from different cities for whole genome sequencing. Phylogenetic and variation analyses were performed based on the obtained sequences, HAdV-7 prototype strain Gomen(AY594255), vaccine strains(AY495969 and AY594256) and representative sequences of strains. The phylogenetic trees constructed based on whole genome sequences, major capsid protein genes(hexon, penton base and fiber) and the early genes(E1, E2, E3 and E4) were not completely consistent. The HAdV-7 strains obtained in this study always clustered with most of the circulating strains worldwide from the 1980 s to the present. Compared with the HAdV-7 prototype strain Gomen(AY594255), some amino acid mutations in loop1 and loop2 of hexon and the RGD loop region of the penton base gene were observed. Recombination analysis showed that partial regions of 55 k Da protein and 100 kDa hexon-assembly associated protein genes among all HAdV-7 strains in this study were from HAdV-16 and HAdV-3, respectively. Our study demonstrated the molecular evolution characteristics of HAdV-7 strains circulating in China and provided basic reference data for the prevention, control and vaccine development of HAdV-7. 相似文献
77.
为探究北盘江大峡谷流域内浮游生物群落组成及多样性情况,于2017年10月对该流域进行浮游生物调查及多样性分析.结果表明该流域内浮游植物共有7门40属61种、其中绿藻门、硅藻门和蓝藻门种类分别为21种、19种和12种,分别占到34.43%、31.15%和19.67%,浮游植物平均密度为4 298.33(375~9 500)ind./L,Shannon-Wiener藻类生物多样性指数均处于1和2之间;浮游动物3门12属21种,其中轮虫类、枝角类和桡足类种类分别为11种、5种和5种,各占到52.38%、23.81%和23.81%,浮游动物平均密度为436.11(55~1 437.5)ind./L,Shannon-Wiener生物多样性指数均处于1和2之间.本研究结果该地区水生生物资源积累了本底资料. 相似文献
78.
Mengtian Fan Jinghong Wu Xian Li Yingjiu Jiang Xiaowen Wang Mengjun Bie Yaguang Weng Sicheng Chen Bin Chen Liqin An Menghao Zhang Gaigai Huang Mengying Zhu Qiong Shi 《Journal of cellular and molecular medicine》2021,25(1):132-146
It has been reported that chemokine CX3CL1 can regulate various tumours by binding to its unique receptor CX3CR1. However, the effect of CX3CL1-CX3CR1 on the lung adenocarcinoma and lung squamous cell carcinoma is still unclear. Here, we showed that CX3CL1 can further invasion and migration of lung adenocarcinoma A549 and lung squamous cell carcinoma H520. In addition, Western blot and immunofluorescence test indicated CX3CL1 up-regulated the phosphorylation level of cortactin, which is a marker of cell pseudopodium. Meanwhile, the phosphorylation levels of c-Src and c-Abl, which are closely related to the regulation of cortactin phosphorylation, are elevated. Nevertheless, the src/abl inhibitor bosutinib and mutations of cortactin phosphorylation site could inhibit the promotion effect of CX3CL1 on invasion and migration of A549 and H520. Moreover, these results of MTT, Hoechst staining and Western blot suggested that CX3CL1 had no effect on the proliferation and apoptosis of A549 and H520 in vitro. The effects of CX3CL1 were also verified by the subcutaneous tumour formation in nude mice, which showed that it could promote proliferation and invasion of A549 in vivo. In summary, our results indicated that CX3CL1 furthered invasion and migration in lung cancer cells partly via activating cortactin, and CX3CL1 may be a potential molecule in regulating the migration and invasion of lung cancer. 相似文献
79.
Ovarian cancer (OC) remains one of the most lethal gynecological malignancies. The unfavourable prognosis is mainly due to the lack of early-stage diagnosis, drug resistance and recurrence. Therefore, it needs to investigate the mechanism of OC tumorigenesis and identify effective biomarkers for the clinical diagnosis. It is reported that long noncoding RNAs (lncRNAs) play important roles during the tumorigenesis of OC. Therefore, the present study aimed to study the role and clinical significance of LncRNAs ATB (lnc-ATB) in the development and progression of OC. In our research, lnc-ATB expression in OC tissues was elevated compared with adjacent normal tissues and high expression of lnc-ATB was associated with poor outcomes of OC patients. The silencing of lnc-ATB blocked cell proliferation, invasion and migration in SKOV3 and A2780 cells. RNA immunoprecipitation and RNA pull-down results showed that lnc-ATB positively regulated the expression of EZH2 via directly interacting with EZH2. Besides, the overexpression of EZH2 partly rescued lnc-ATB silencing-inducing inhibition of cell proliferation, invasion and migration. Chromatin immunoprecipitation assay results demonstrated that the silencing of lnc-ATB reduced the occupancy of caudal-related homeobox protein 1, Forkhead box C1, Large tumour suppressor kinase 2, cadherin-1 and disabled homolog 2 interacting protein promoters on EZH2 and H3K27me3. These data revealed the oncogenic of lnc-ATB and provided a novel biomarker for OC diagnosis. Furthermore, these findings indicated the mechanism of lnc-ATB functioning in the progression of OC, which provided a new target for OC therapy. 相似文献
80.
Xiang Gao Zhaoxia Zhu Haiyan Xu Litao Liu Jing An Boyang Ji Shuifeng Ye 《Environmental microbiology》2021,23(2):713-727