全文获取类型
收费全文 | 8857篇 |
免费 | 899篇 |
国内免费 | 3篇 |
出版年
2023年 | 41篇 |
2022年 | 111篇 |
2021年 | 216篇 |
2020年 | 119篇 |
2019年 | 153篇 |
2018年 | 182篇 |
2017年 | 170篇 |
2016年 | 253篇 |
2015年 | 452篇 |
2014年 | 471篇 |
2013年 | 570篇 |
2012年 | 753篇 |
2011年 | 698篇 |
2010年 | 448篇 |
2009年 | 455篇 |
2008年 | 547篇 |
2007年 | 572篇 |
2006年 | 531篇 |
2005年 | 457篇 |
2004年 | 490篇 |
2003年 | 385篇 |
2002年 | 359篇 |
2001年 | 101篇 |
2000年 | 87篇 |
1999年 | 97篇 |
1998年 | 90篇 |
1997年 | 56篇 |
1996年 | 56篇 |
1995年 | 53篇 |
1994年 | 44篇 |
1993年 | 49篇 |
1992年 | 51篇 |
1991年 | 52篇 |
1990年 | 54篇 |
1989年 | 49篇 |
1988年 | 31篇 |
1987年 | 38篇 |
1986年 | 40篇 |
1985年 | 46篇 |
1984年 | 39篇 |
1983年 | 32篇 |
1982年 | 26篇 |
1981年 | 25篇 |
1980年 | 15篇 |
1979年 | 16篇 |
1974年 | 21篇 |
1973年 | 18篇 |
1972年 | 15篇 |
1969年 | 13篇 |
1967年 | 12篇 |
排序方式: 共有9759条查询结果,搜索用时 68 毫秒
921.
Duplication of the MECP2 region is a frequent cause of severe mental retardation and progressive neurological symptoms in males
下载免费PDF全文
![点击此处可从《American journal of human genetics》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Van Esch H Bauters M Ignatius J Jansen M Raynaud M Hollanders K Lugtenberg D Bienvenu T Jensen LR Gecz J Moraine C Marynen P Fryns JP Froyen G 《American journal of human genetics》2005,77(3):442-453
Loss-of-function mutations of the MECP2 gene at Xq28 are associated with Rett syndrome in females and with syndromic and nonsyndromic forms of mental retardation (MR) in males. By array comparative genomic hybridization (array-CGH), we identified a small duplication at Xq28 in a large family with a severe form of MR associated with progressive spasticity. Screening by real-time quantitation of 17 additional patients with MR who have similar phenotypes revealed three more duplications. The duplications in the four patients vary in size from 0.4 to 0.8 Mb and harbor several genes, which, for each duplication, include the MR-related L1CAM and MECP2 genes. The proximal breakpoints are located within a 250-kb region centromeric of L1CAM, whereas the distal breakpoints are located in a 300-kb interval telomeric of MECP2. The precise size and location of each duplication is different in the four patients. The duplications segregate with the disease in the families, and asymptomatic carrier females show complete skewing of X inactivation. Comparison of the clinical features in these patients and in a previously reported patient enables refinement of the genotype-phenotype correlation and strongly suggests that increased dosage of MECP2 results in the MR phenotype. Our findings demonstrate that, in humans, not only impaired or abolished gene function but also increased MeCP2 dosage causes a distinct phenotype. Moreover, duplication of the MECP2 region occurs frequently in male patients with a severe form of MR, which justifies quantitative screening of MECP2 in this group of patients. 相似文献
922.
Sullivan A 《American journal of physical anthropology》2005,128(2):252-272
This paper presents three distinct models for the development of acquired anemia: iron-deficiency anemia produced by the inadequate intake and/or absorption of iron, the anemia of chronic disease (ACD) caused by the body's natural iron-withholding defense against microbial invaders, and megaloblastic anemia caused by insufficient intake and/or absorption of vitamin B(12) or folic acid. These etiological models are used to interpret the distribution and etiology of anemia among adult individuals interred at the Medieval Gilbertine Priory of St. Andrew, Fishergate, York (n = 147). This bioarchaeological analysis uncovered not only a strong relationship between decreasing status and increasing prevalence of anemia for both men and women, but also identified clear sex-based differences at this site. Within the high-status group, blood and iron loss as a result of rampant parasitism likely produced an environment ripe for the development of iron-deficiency anemia, while the parasitic consumption of vitamin B(12) may have caused occasional cases of megaloblastic anemia. As status decreases, the interpretation of anemia becomes more complex, with megaloblastic anemia and ACD emerging as viable, potentially heavy contributors to the anemia experiences of low-status people at St. Andrew's. Apart from status effects, women (especially young women) are disproportionately affected by anemia when compared to men within their own status group and, on average, are also more likely to have experienced anemia than their male peers from other status groups. This suggests that high iron-demand reproductive functions helped to make iron-deficiency anemia a chronic condition in many women's lives irrespective of their status affiliation. 相似文献
923.
Loss of CD127 expression defines an expansion of effector CD8+ T cells in HIV-infected individuals 总被引:15,自引:0,他引:15
Paiardini M Cervasi B Albrecht H Muthukumar A Dunham R Gordon S Radziewicz H Piedimonte G Magnani M Montroni M Kaech SM Weintrob A Altman JD Sodora DL Feinberg MB Silvestri G 《Journal of immunology (Baltimore, Md. : 1950)》2005,174(5):2900-2909
The immunodeficiency that follows HIV infection is related to the virus-mediated killing of infected CD4(+) T cells, the chronic activation of the immune system, and the impairment of T cell production. In this study we show that in HIV-infected individuals the loss of IL-7R (CD127) expression defines the expansion of a subset of CD8(+) T cells, specific for HIV as well as other Ags, that show phenotypic (i.e., loss of CCR7 and CD62 ligand expression with enrichment in activated and/or proliferating cells) as well as functional (i.e., production of IFN-gamma, but not IL-2, decreased ex vivo proliferative potential and increased susceptibility to apoptosis) features of effector T cells. Importantly, in HIV-infected individuals the levels of CD8(+)CD127(-) T cells are directly correlated with the main markers of disease progression (i.e., plasma viremia and CD4(+) T cell depletion) as well as with the indices of overall T cell activation. In all, these results identify the expansion of CD8(+)CD127(-) effector-like T cells as a novel feature of the HIV-associated immune perturbation. Further studies are thus warranted to determine whether measurements of CD127 expression on CD8(+) T cells may be useful in the clinical management of HIV-infected individuals. 相似文献
924.
Nucleotide-binding oligomerization domain-2 modulates specific TLR pathways for the induction of cytokine release 总被引:13,自引:0,他引:13
Netea MG Ferwerda G de Jong DJ Jansen T Jacobs L Kramer M Naber TH Drenth JP Girardin SE Kullberg BJ Adema GJ Van der Meer JW 《Journal of immunology (Baltimore, Md. : 1950)》2005,174(10):6518-6523
The recognition of peptidoglycan by cells of the innate immune system has been controversial; both TLR2 and nucleotide-binding oligomerization domain-2 (NOD2) have been implicated in this process. In the present study we demonstrate that although NOD2 is required for recognition of peptidoglycan, this leads to strong synergistic effects on TLR2-mediated production of both pro- and anti-inflammatory cytokines. Defective IL-10 production in patients with Crohn's disease bearing loss of function mutations of NOD2 may lead to overwhelming inflammation due to a subsequent Th1 bias. In addition to the potentiation of TLR2 effects, NOD2 is a modulator of signals transmitted through TLR4 and TLR3, but not through TLR5, TLR9, or TLR7. Thus, interaction between NOD2 and specific TLR pathways may represent an important modulatory mechanism of innate immune responses. 相似文献
925.
MARCO is the major binding receptor for unopsonized particles and bacteria on human alveolar macrophages 总被引:3,自引:0,他引:3
Arredouani MS Palecanda A Koziel H Huang YC Imrich A Sulahian TH Ning YY Yang Z Pikkarainen T Sankala M Vargas SO Takeya M Tryggvason K Kobzik L 《Journal of immunology (Baltimore, Md. : 1950)》2005,175(9):6058-6064
Alveolar macrophages (AMs) avidly bind and ingest inhaled environmental particles and bacteria. To identify the particle binding receptor(s) on human AMs, we used functional screening of anti-human AM hybridomas and isolated a mAb, PLK-1, which inhibits AM binding of unopsonized particles (e.g., TiO2, latex beads; 63 +/- 5 and 67 +/- 4% inhibition, respectively, measured by flow cytometry; n = 11) and unopsonized bacteria ( approximately 84 and 41% inhibition of Escherichia coli and Staphylococcus aureus binding by mAb PLK-1, respectively). The PLK-1 Ag was identified as the human class A scavenger receptor (SR) MARCO (macrophage receptor with collagenous structure) by observing specific immunolabeling of COS cells transfected with human MARCO (but not SR-AI/II) cDNA and by immunoprecipitation by PLK-1 of a protein of appropriate molecular mass (approximately 70 kDa) from both normal human bronchoalveolar lavage cells (>90% AMs) and human MARCO-transfected COS cells. PLK-1 also specifically inhibited particle binding by COS cells, only after transfection with human MARCO cDNA. Immunostaining showed specific labeling of AMs within human lung tissue, bronchoalveolar lavage samples, as well as macrophages in other sites (e.g., lymph node and liver). Using COS transfectants with different truncated forms of MARCO, allowed epitope mapping for the PLK-1 Ab to MARCO domain V between amino acid residues 420 and 431. A panel of Abs to various SRs identified expression on AMs, but failed to inhibit TiO2 or S. aureus binding. The data support a dominant role for MARCO in the human AM defense against inhaled particles and pathogens. 相似文献
926.
Molecular basis for the potency of IL-10-deficient dendritic cells as a highly efficient APC system for activating Th1 response 总被引:2,自引:0,他引:2
927.
We report a novel developmental mechanism. Anterior-posterior positional information for the vertebrate trunk is generated by sequential interactions between a timer in the early non-organiser mesoderm and the organiser. The timer is characterised by temporally colinear activation of a series of Hox genes in the early ventral and lateral mesoderm (i.e., the non-organiser mesoderm) of the Xenopus gastrula. This early Hox gene expression is transient, unless it is stabilised by signals from the Spemann organiser. The non-organiser mesoderm and the Spemann organiser undergo timed interactions during gastrulation which lead to the formation of an anterior-posterior axis and stable Hox gene expression. When separated from each other, neither non-organiser mesoderm nor the Spemann organiser is able to induce anterior-posterior pattern formation of the trunk. We present a model describing that convergence and extension continually bring new cells from the non-organiser mesoderm within the range of organiser signals and thereby create patterned axial structures. In doing so, the age of the non-organiser mesoderm, but not the age of the organiser, defines positional values along the anterior-posterior axis. We postulate that the temporal information from the non-organiser mesoderm is linked to mesodermal Hox expression. 相似文献
928.
GATA4 is a dosage-sensitive regulator of cardiac morphogenesis 总被引:15,自引:0,他引:15
929.
Human breast epithelium and the stroma are separated by a layer of myoepithelial (ME) cells and basement membrane, whose disruption is a prerequisite for tumor invasion. The dissolution of the basement membrane is traditionally attributed primarily to an over-production of proteolytic enzymes by the tumor or the surrounding stromal cells. The results from matrix metalloproteinase inhibitor clinical trials, however, suggest that this "protease-centered" hypothesis is inadequate to completely reflect the molecular mechanisms of tumor invasion. The causes and signs of ME cell layer disruption are currently under-explored. Our studies revealed that a subset of pre- and micro-invasive tumors contained focal disruptions in the ME cell layers. These disruptions were associated with immunohistochemical and genetic alterations in the overlying tumor cells, including the loss of estrogen receptor expression, a higher frequency of loss of heterozygosity, and a higher expression of cell cycle, angiogenesis, and invasion-related genes. Focal ME layer disruptions were also associated with a higher rate of epithelial proliferation and leukocyte infiltration. We propose the novel hypothesis that a localized death of ME cells and immunoreactions that accompany an external environmental insult or internal genetic alterations are triggering factors for ME layer disruptions, basement membrane degradation, and subsequent tumor progression and invasion. 相似文献
930.