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51.
Coating of electrospun poly(lactic‐co‐glycolic acid) nanofibers with willemite bioceramic: improvement of bone reconstruction in rat model 下载免费PDF全文
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Mohsen Rashidi Alireza Ahmadzadeh Seyed Ali Ziai Mahsa Narenji Hamidreza Jamshidi 《In vitro cellular & developmental biology. Animal》2017,53(1):7-11
Cytotoxicity of umbelliprenin has been found in various cancer cell lines such as, prostate, breast, CLL, and skin. Encapsulating chemotherapeutic agents with nanoliposomes have been resulted in improved cytotoxicity effects than their free forms. However, whether nanoliposomal form of umbelliprenin could have higher cytotoxic effect than free umbelliprenin is not clarified yet. After synthesizing umbelliprenin, different concentrations (3, 6, 12, 25, 50, 100, 200 μg/ml) applied on the mouse mammary carcinoma cell line (4T1) for 24, 48, and 72 h at 37°C. Afterwards, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was performed to analyze cytotoxicity. MTT assay results showed that IC50 of umbelliprenin in dimethyl sulfoxide (DMSO) (30.92, 30.64, and 62.23 for 24, 48, 72 h incubation, respectively) decreased (5.8, 5.0, 3.5 for 24, 48, 72 h incubation, respectively) when encapsulated with nanoliposomes. Nanoliposomal umbelliprenin cytotoxicity affected cell viability in concentration and time-dependent manner. Our study recommended nanoliposomal umbelliprenin as the most effective chemotherapeutic agent against the mouse mammary carcinoma cell line viability. Future in vivo studies and clinical trials are needed. 相似文献
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Sialic acid is a cellular receptor for coxsackievirus A24 variant, an emerging virus with pandemic potential 总被引:1,自引:0,他引:1
Binding to target cell receptors is a critical step in the virus life cycle. Coxsackievirus A24 variant (CVA24v) has pandemic potential and is a major cause of acute hemorrhagic conjunctivitis, but its cellular receptor has hitherto been unknown. Here we show that CVA24v fails to bind to and infect CHO cells defective in sialic acid expression. Binding of CVA24v to and infection of corneal epithelial cells are efficiently inhibited by treating cells with a sialic acid-cleaving enzyme or sialic acid-binding lectins and by treatment of the virus with soluble, multivalent sialic acid. Protease treatment of cells efficiently inhibited virus binding, suggesting that the receptor is a sialylated glycoprotein. Like enterovirus type 70 and influenza A virus, CVA24v can cause pandemics. Remarkably, all three viruses use the same receptor. Since several unrelated viruses with tropism for the eye use this receptor, sialic acid-based antiviral drugs that prevent virus entry may be useful for topical treatment of such infections. 相似文献
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Gorzi Ali Jamshidi Firooz Rahmani Ahmad Krause Neto Walter 《Molecular biology reports》2020,47(7):5305-5314
Molecular Biology Reports - The neuromuscular junction underwent adaptations to meet the demands of muscles following increased muscle activity. This study aimed to investigate the effects of... 相似文献
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Ali Ebrahim Eivind Almaas Eugen Bauer Aarash Bordbar Anthony P Burgard Roger L Chang Andreas Dräger Iman Famili Adam M Feist Ronan MT Fleming Stephen S Fong Vassily Hatzimanikatis Markus J Herrgård Allen Holder Michael Hucka Daniel Hyduke Neema Jamshidi Sang Yup Lee Nicolas Le Novère Joshua A Lerman Nathan E Lewis Ding Ma Radhakrishnan Mahadevan Costas Maranas Harish Nagarajan Ali Navid Jens Nielsen Lars K Nielsen Juan Nogales Alberto Noronha Csaba Pal Bernhard O Palsson Jason A Papin Kiran R Patil Nathan D Price Jennifer L Reed Michael Saunders Ryan S Senger Nikolaus Sonnenschein Yuekai Sun Ines Thiele 《Molecular systems biology》2015,11(10)
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Gholamreza?Daryabor Mahdi?Mahmoudi Ahmadreza?Jamshidi Keramat?Nourijelyani Aliakbar?Amirzargar Nooshin?Ahmadzadeh Elham?Farhadi Mohammad?Hossein?NicknamEmail author 《European cytokine network》2014,25(1):24-29
Introduction
The result of recent genome-wide association studies revealed that, in addition to HLA-B27, a few non-HLA genes are associated with susceptibility to ankylosing spondylitis (AS) in Caucasian populations. According to these studies, IL-23R is one of the genes that is associated with AS. In this study, we evaluated five important single nucleotide polymorphisms (SNPs) of the IL-23R gene which confers susceptibility to AS, and its effects on the severity of the disease in HLA-B27 positive and negative patients and several subtypes of HLA-B27.Materials and methods
The study population consisted of 294 AS patients and 352 age-, sex-, and ethnicity-matched healthy controls. All patients were examined by rheumatologists, and met modified,NewYork criteria for the disease. Five SNPs (rs1004819, rs11209032, rs1495965, rs11465804, and rs1004819) of the IL-23R gene were genotyped using the Real-Time PCR TaqMan genotyping method.Results
We found that only rs1004819 has a significant association with AS, and that the remaining four SNP alleles are not associated with AS. Also, there was no association between these five polymorphisms and BASDAI, BASFI, and BASMI indices. Two haplotypes, ACGAT and ACGAG, were found to be associated with the heritability of AS. In addition, two significant, protective diplotypes (D8, \(\frac{{GCGAG}}{{GTGGG}}\); and D9, \(\frac{{ACGAG}}{{GCGAG}}\)) were discovered.Conclusion
This study supported our previous findings regarding the differences between the genetic patterns of AS in Iranian patients compared with those in other parts of the world.57.
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Fatemeh Jamshidi‐Adegani Ehsan Seyedjafari Nematollah Gheibi Masoud Soleimani Mehdi Sahmani 《Biotechnology progress》2016,32(4):990-997
In this study, prevention of the adhesion bands and inflammatory features has been investigated using poly (lactic‐co‐glycolic acid)‐ibuprofen (PLGA‐IB) nanofibrous meshes in a mice model. To find the optimized membrane for prevention of postoperative adhesion bands, we have compared PLGA‐IB group with PLGA, IB, and control groups in a mice adhesion model. Two scoring adhesion systems were used to represent the outcome. According to the results obtained in this study, the PLGA‐IB nanofiber membrane showed a greater reduction in adhesion band than other groups. In conclusion, among FDA‐approved polymers and drugs, PLGA‐IB meshes could be applicable as a potential candidate for prevention of postoperative abdominal inflammation and adhesion bands formation. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:990–997, 2016 相似文献
60.
Birgit Harbeck Sven Danneberg Amir-Hossein Rahvar Christian S. Haas Hendrik Lehnert Peter Kropp Heiner Mönig 《Applied psychophysiology and biofeedback》2016,41(3):341-347
Hydrocortisone (HC) substitution is essential in the treatment for patients with adrenal insufficiency (AI). Current replacement regimens however only incompletely mimic the physiological circadian rhythm of cortisol secretion, thereby resulting in subclinical temporary hypo- and hypercortisolism. Several studies point toward impairment of cognitive functions under these conditions, in part due to affected catecholamine secretion. Aim of this study was to evaluate the influence of long-term versus short-term HC replacement therapy on the adrenomedullary system and cognitive functions. Fourteen patients with primary or secondary AI were divided into two groups, depending on the duration of disease and HC replacement therapy (<15 years). All subjects underwent standardized neurocognitive testing; in addition, cortisol and catecholamine levels as well as physiological parameters and quality of life (QoL) were assessed. Patients with HC replacement therapy ≥15 years (n = 7) received significantly higher equivalent glucocorticoid doses than those with a shorter lasting therapy (n = 7; p = 0.048). Neuropsychological tests, QoL, physiological parameters, and cortisol levels did not differ significantly between both groups. Of note, norepinephrine levels were significantly lower in patients on short-term HC replacement therapy (p = 0.025). However, there were no significant differences in catecholamines with respect to the underlying pathophysiology, gender, or age. Irrespective of the duration of use, male patients scored significantly better for single aspects of QoL, whereas females performed significantly better in the attention test. Overall, we showed that duration of cortisol replacement therapy may have an impact on catecholamine release, but does not seem to affect cognitive functions and QoL. 相似文献