全文获取类型
收费全文 | 5088篇 |
免费 | 366篇 |
国内免费 | 1篇 |
专业分类
5455篇 |
出版年
2023年 | 35篇 |
2022年 | 98篇 |
2021年 | 146篇 |
2020年 | 91篇 |
2019年 | 142篇 |
2018年 | 184篇 |
2017年 | 157篇 |
2016年 | 192篇 |
2015年 | 265篇 |
2014年 | 268篇 |
2013年 | 392篇 |
2012年 | 379篇 |
2011年 | 423篇 |
2010年 | 270篇 |
2009年 | 236篇 |
2008年 | 237篇 |
2007年 | 238篇 |
2006年 | 182篇 |
2005年 | 175篇 |
2004年 | 142篇 |
2003年 | 123篇 |
2002年 | 116篇 |
2001年 | 90篇 |
2000年 | 108篇 |
1999年 | 81篇 |
1998年 | 58篇 |
1997年 | 31篇 |
1996年 | 31篇 |
1995年 | 27篇 |
1994年 | 15篇 |
1993年 | 20篇 |
1992年 | 25篇 |
1991年 | 21篇 |
1990年 | 15篇 |
1989年 | 26篇 |
1988年 | 17篇 |
1987年 | 15篇 |
1986年 | 21篇 |
1985年 | 16篇 |
1984年 | 27篇 |
1983年 | 23篇 |
1982年 | 25篇 |
1981年 | 14篇 |
1980年 | 34篇 |
1979年 | 29篇 |
1978年 | 27篇 |
1977年 | 25篇 |
1976年 | 13篇 |
1974年 | 12篇 |
1973年 | 12篇 |
排序方式: 共有5455条查询结果,搜索用时 0 毫秒
991.
Gregory J. Crowther Heidi K. Hillesland Katelyn R. Keyloun Molly C. Reid Maria Jose Lafuente-Monasterio Sonja Ghidelli-Disse Stephen E. Leonard Panqing He Jackson C. Jones Mallory M. Krahn Jack S. Mo Kartheek S. Dasari Anna M. W. Fox Markus Boesche Majida El Bakkouri Kasey L. Rivas Didier Leroy Raymond Hui Gerard Drewes Dustin J. Maly Wesley C. Van Voorhis Kayode K. Ojo 《PloS one》2016,11(3)
In 2010 the identities of thousands of anti-Plasmodium compounds were released publicly to facilitate malaria drug development. Understanding these compounds’ mechanisms of action—i.e., the specific molecular targets by which they kill the parasite—would further facilitate the drug development process. Given that kinases are promising anti-malaria targets, we screened ~14,000 cell-active compounds for activity against five different protein kinases. Collections of cell-active compounds from GlaxoSmithKline (the ~13,000-compound Tres Cantos Antimalarial Set, or TCAMS), St. Jude Children’s Research Hospital (260 compounds), and the Medicines for Malaria Venture (the 400-compound Malaria Box) were screened in biochemical assays of Plasmodium falciparum calcium-dependent protein kinases 1 and 4 (CDPK1 and CDPK4), mitogen-associated protein kinase 2 (MAPK2/MAP2), protein kinase 6 (PK6), and protein kinase 7 (PK7). Novel potent inhibitors (IC50 < 1 μM) were discovered for three of the kinases: CDPK1, CDPK4, and PK6. The PK6 inhibitors are the most potent yet discovered for this enzyme and deserve further scrutiny. Additionally, kinome-wide competition assays revealed a compound that inhibits CDPK4 with few effects on ~150 human kinases, and several related compounds that inhibit CDPK1 and CDPK4 yet have limited cytotoxicity to human (HepG2) cells. Our data suggest that inhibiting multiple Plasmodium kinase targets without harming human cells is challenging but feasible. 相似文献
992.
Baioumy Asmaa Ali Swelim Hamdy Hamed Ibrahim Ahmed Adly Mohamed Fatma El‑Sayed Marzouk Aleya Soliman El‑Alfy Sherif Helmy 《Experimental & applied acarology》2022,86(4):609-610
Experimental and Applied Acarology - 相似文献
993.
994.
Pavel Veprek Jan Jezek Jirí Velek Hatem Tallima Mona Montash Rashika El Ridi 《Journal of peptide science》2004,10(6):350-362
Four monoepitopic MAPs (MAP A, B, C and E) and one bis-diepitopic MAP B-E derived fromthe primary sequence of Schistosoma mansoni glyceraldehyde 3-phosphate dehydrogenase, previously tested in BALB/c mice, were examined for their immunogenicity and protective capacity in C57BL/6 mice. Despite multimerization into MAPs, MAP Aand MAP C were poorly immunogenic. In contrast toBALB/c mice, MAP E was non-immunogenic in C57BL/6 mice. Peptide B in the form of MAP B orbis-diepitopic MAPB-E elicited immune responses in C57BL/6 mice that were associated with a significant decrease in worm burden. The MAPs were prepared by the stepwise solid-phase peptide synthesis using Boc/Bzl chemistry, successfully purified on the RP-HPLC column and characterized by RP-HPLC, HPCE and MALDI-TOF MS techniques. A general strategy for MAPs purification is discussed here and the purification of MAP Band MAP E is documented in detail. 相似文献
995.
996.
997.
998.
J. Gutt M. C. Alvaro A. Barco A. Böhmer A. Bracher B. David C. De Ridder B. Dorschel M. Eléaume D. Janussen D. Kersken P. J. López-González I. Martínez-Baraldés M. Schröder A. Segelken-Voigt N. Teixidó 《Polar Biology》2016,39(5):829-849
The Southern Ocean ecosystem at the Antarctic Peninsula has steep natural environmental gradients, e.g. in terms of water masses and ice cover, and experiences regional above global average climate change. An ecological macroepibenthic survey was conducted in three ecoregions in the north-western Weddell Sea, on the continental shelf of the Antarctic Peninsula in the Bransfield Strait and on the shelf of the South Shetland Islands in the Drake Passage, defined by their environmental envelop. The aim was to improve the so far poor knowledge of the structure of this component of the Southern Ocean ecosystem and its ecological driving forces. It can also provide a baseline to assess the impact of ongoing climate change to the benthic diversity, functioning and ecosystem services. Different intermediate-scaled topographic features such as canyon systems including the corresponding topographically defined habitats ‘bank’, ‘upper slope’, ‘slope’ and ‘canyon/deep’ were sampled. In addition, the physical and biological environmental factors such as sea-ice cover, chlorophyll-a concentration, small-scale bottom topography and water masses were analysed. Catches by Agassiz trawl showed high among-station variability in biomass of 96 higher systematic groups including ecological key taxa. Large-scale patterns separating the three ecoregions from each other could be correlated with the two environmental factors, sea-ice and depth. Attribution to habitats only poorly explained benthic composition, and small-scale bottom topography did not explain such patterns at all. The large-scale factors, sea-ice and depth, might have caused large-scale differences in pelagic benthic coupling, whilst small-scale variability, also affecting larger scales, seemed to be predominantly driven by unknown physical drivers or biological interactions. 相似文献
999.
BackgroundPrivacy legislation in most jurisdictions allows the disclosure of health data for secondary purposes without patient consent if it is de-identified. Some recent articles in the medical, legal, and computer science literature have argued that de-identification methods do not provide sufficient protection because they are easy to reverse. Should this be the case, it would have significant and important implications on how health information is disclosed, including: (a) potentially limiting its availability for secondary purposes such as research, and (b) resulting in more identifiable health information being disclosed. Our objectives in this systematic review were to: (a) characterize known re-identification attacks on health data and contrast that to re-identification attacks on other kinds of data, (b) compute the overall proportion of records that have been correctly re-identified in these attacks, and (c) assess whether these demonstrate weaknesses in current de-identification methods.ConclusionsThe current evidence shows a high re-identification rate but is dominated by small-scale studies on data that was not de-identified according to existing standards. This evidence is insufficient to draw conclusions about the efficacy of de-identification methods. 相似文献
1000.
El Fitori J Kleeff J Giese NA Guweidhi A Bosserhoff AK Büchler MW Friess H 《Cancer cell international》2005,5(1):3