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排序方式: 共有1826条查询结果,搜索用时 31 毫秒
881.
van Koolwijk LM Ramdas WD Ikram MK Jansonius NM Pasutto F Hysi PG Macgregor S Janssen SF Hewitt AW Viswanathan AC ten Brink JB Hosseini SM Amin N Despriet DD Willemse-Assink JJ Kramer R Rivadeneira F Struchalin M Aulchenko YS Weisschuh N Zenkel M Mardin CY Gramer E Welge-Lüssen U Montgomery GW Carbonaro F Young TL;DCCT/EDIC Research Group Bellenguez C McGuffin P Foster PJ Topouzis F Mitchell P Wang JJ Wong TY Czudowska MA Hofman A Uitterlinden AG Wolfs RC de Jong PT Oostra BA 《PLoS genetics》2012,8(5):e1002611
Intraocular pressure (IOP) is a highly heritable risk factor for primary open-angle glaucoma and is the only target for current glaucoma therapy. The genetic factors which determine IOP are largely unknown. We performed a genome-wide association study for IOP in 11,972 participants from 4 independent population-based studies in The Netherlands. We replicated our findings in 7,482 participants from 4 additional cohorts from the UK, Australia, Canada, and the Wellcome Trust Case-Control Consortium 2/Blue Mountains Eye Study. IOP was significantly associated with rs11656696, located in GAS7 at 17p13.1 (p = 1.4×10−8), and with rs7555523, located in TMCO1 at 1q24.1 (p = 1.6×10−8). In a meta-analysis of 4 case-control studies (total N = 1,432 glaucoma cases), both variants also showed evidence for association with glaucoma (p = 2.4×10−2 for rs11656696 and p = 9.1×10−4 for rs7555523). GAS7 and TMCO1 are highly expressed in the ciliary body and trabecular meshwork as well as in the lamina cribrosa, optic nerve, and retina. Both genes functionally interact with known glaucoma disease genes. These data suggest that we have identified two clinically relevant genes involved in IOP regulation. 相似文献
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The ladybird beetle Clitostethus arcuatus Rossi (Coleoptera: Coccinellidae) is one of the most effective predators of the greenhouse whitefly, Trialeurodes vaporariorum Westwood, and the ash whitefly, Siphoninus phillyreae Haliday (Hem: Aleyrodidae). Two stock cultures of C. arcuatus were established under controlled conditions (25 ± 2 °C, 65 ± 5%RH and L–D:16–8), one using T. vaporariorum and one using S. phillyreae as prey. Newly laid C. arcuatus eggs from each culture were evaluated for immature survival. Thirty pairs of C. arcuatus (24 h-old) were selected for studying the reproductive life history of C. arcuatus on the two hosts. Results showed that the period between oviposition and hatch did not show significant difference on different prey. Duration of different instars stages of C. arcuatus differed significantly, except second instars, which was 4 days for both diets. Pupal period differed significantly between the two prey types. Egg hatch was 95% and 91.7% for adults fed on T. vaporariorum and S. phillyreae, respectively. There was a significant difference among some treatments for gross fecundity rate and net reproductive rate (R0), indicating that prey had a significant impact on the biological activities of C. arcuatus. The intrinsic rate (rm) and finite rate of increase (λ) were 0.063 and 0.078, respectively, for T. vaporariorum, and 1.0026 and, 1.08, respectively, for S. phillyreae. 相似文献
884.
Staphylococcal infections have high occurrence in Jordanian patients. This study was carried out to determine the rates of high- and low-level mupirocin resistance (MupH and MupL) among staphylococci with the molecular characterization. Two hundred and thirty-two non-duplicate Staphylococcus spp. isolated from different clinical specimens were tested for mupirocin susceptibility using disk diffusion method and minimum inhibitory concentration (MIC). Resistance genes and clone relatedness was studied using polymerase chain reaction (PCR) and enterobacterial repetitive intergenic consensus primers (Eric-PCR) for the latter. Plasmid curing was performed to determine the genetic location of MupA gene. Among the 232 strains, 144 (62%) were methicillin-resistant Staphylococcus aureus (MRSA), 33 (14.2%) methicillin-susceptible Staphylococcus aureus (MSSA) and 55 (23.7%) were of other coagulase-negative Staphylococcus spp. (CoNS). Of all strains tested, only 6 (2.6%) were mupirocin resistant. MecA gene was detected in both MupL and MupH strains but MupA gene was only detected in MupH. Plasmid curing improved the plasmidic location of MupA gene. Molecular typing by Eric-PCR method revealed heterogenicity of the genetic make up of our MupL and MupH strains. Staphylococci with MupA-carrying genes are present in Jordanian hospitals, but thank to the limited use of mupirocin, they remain rare. 相似文献
885.
Neoechinorhynchus manubrianus Amin, Ha & Ha, 2011 (Acanthocephala: Neoechinorhynchidae) (formerly Neoechinorhynchus manubriensis Amin, Ha & Ha, 2011), was recently described based on optical microscopy of four males and two females (none was gravid) from caroun croaker, Johnius carouna (Cuvier), flower croaker, Nibea albiflora (Richardson), and silver croaker, Pennabia argentata (Houttuyen) (Sciaenidae) in Halong Bay, Vietnam. Subsequently, many more specimens became available from N. albiflora that were studied using SEM. SEM studies showed many additional features that were not possible to discern with optical microscopy. These included the prominent angulation of the anterior trunk, the presence of (1) anterio-dorsal and (2) undulating mid-lateral fin-like protrusions of the body wall, uniquely shaped eggs as well as details of trunk micropores, proboscis, bursa, and female gonopore. Microscopical examination of eggs from the new collection demonstrated the presence of polar prolongation of fertilization membrane which places N. manubriensis in the subgenus Hebesoma. The features of trunk angulation, trunk fins, and egg morphology further distinguish N. manubriensis from all other species of Neoechinorhynchus Stiles and Hassall, 1905 from Vietnam or from any where else in the world. 相似文献
886.
Sarmast MK Salehi H Ramezani A Abolimoghadam AA Niazi A Khosh-Khui M 《Molecular biotechnology》2012,50(3):181-188
Randomly amplified polymorphic DNA (RAPD) was used as a tool to assess the genetic fidelity of in vitro propagated Araucaria excelsa R. Br. var. glauca with explants taken from orthotropic stem along with their related mother plants after treatment with
kinetin, 2iP, BA (0.02–0.26 mg/l) and TDZ (0.001–1 mg/l) to produce axillary shoots. TDZ and kinetin induced more shoot and
higher length per explant. Results showed a total of 1,676 fragments were generated with 12 RAPD primers in micropropagated
plants and their donor mother plants. The number of loci ranged from 6 in OPB 12–18 in OPY 07 with a size ranging from 250
bp in OPH 19–3500 bp in OPH 11. Cluster analysis of RAPD data using UPGMA (unweighted pair group method with arithmetic average)
revealed more than 92% genetic similarities between tissue cultured plants and their corresponding mother plant measured by
the Jaccard’s similarity coefficient. Similarity matrix and PCoA (two dimensional principal coordinate analysis) resulted
in the same affinity. Primers had shown 36% polymorphism. However, careful monitoring of tissue culture derived plants might
be needed to determine that rooted shoots are adventitious in origin. 相似文献
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Ayaz Najafov Hoang Son Luu Adnan K. Mookhtiar Lauren Mifflin Hong-guang Xia Palak P. Amin Alban Ordureau Huibing Wang Junying Yuan 《Molecular cell》2021,81(2):370-385.e7
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