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排序方式: 共有266条查询结果,搜索用时 46 毫秒
181.
Induction of transient macroapertures in endothelial cells through RhoA inhibition by Staphylococcus aureus factors 总被引:1,自引:0,他引:1 下载免费PDF全文
Boyer L Doye A Rolando M Flatau G Munro P Gounon P Clément R Pulcini C Popoff MR Mettouchi A Landraud L Dussurget O Lemichez E 《The Journal of cell biology》2006,173(5):809-819
The GTPase RhoA is a major regulator of the assembly of actin stress fibers and the contractility of the actomyosin cytoskeleton. The epidermal cell differentiation inhibitor (EDIN) and EDIN-like ADP-ribosyltransferases of Staphylococcus aureus catalyze the inactivation of RhoA, producing actin cable disruption. We report that purified recombinant EDIN and EDIN-producing S. aureus provoke large transcellular tunnels in endothelial cells that we have named macroapertures (MAs). These structures open transiently, followed by the appearance of actin-containing membrane waves extending over the aperture. Disruption of actin cables, either directly or indirectly, through rhoA RNAi knockdown also triggers the formation of MAs. Intoxication of endothelial monolayers by EDIN produces a loss of barrier function and provides direct access of the endothelium basement membrane to S. aureus. 相似文献
182.
Amel Chebel Serge Bauwens Luc-Marie Gerland Aurélie Belleville Iwona Urbanowicz Aude Roborel de Climens Yves Tourneur Wei Wen Chien Régine Catallo Gilles Salles Eric Gilson Martine Ffrench 《Aging cell》2009,8(1):52-64
Normal lymphocytes represent examples of somatic cells that are able to induce telomerase activity when stimulated. As previously reported, we showed that, during lymphocyte long-term culture and repeated stimulations, the appearance of senescent cells is associated with telomere shortening and a progressive drop in telomerase activity. We further showed that this shortening preferentially occured at long telomeres and was interrupted at each stimulation by a transitory increase in telomere length. In agreement with the fact that telomere uncapping triggers lymphocyte senescence, we observed an increase in γ-H2AX and 53BP1 foci as well as in the percentage of cells exhibiting DNA damage foci in telomeres. Such a DNA damage response may be related to the continuous increase of p16 ink4a upon cell stimulation and cell aging. Remarkably, at each stimulation, the expression of shelterin genes, such as hTRF1 , hTANK1 , hTIN2 , hPOT1 and hRAP1 , was decreased. We propose that telomere dysfunction during lymphocyte senescence caused by iterative stimulations does not only result from an excessive telomere shortening, but also from a decrease in shelterin content. These observations may be relevant for T-cell biology and aging. 相似文献
183.
184.
Sabri Denden Ramzi Lakhdar Nadia Leban Jemni Ben Chibani Amel Haj Khelil 《Molecular biotechnology》2010,45(2):111-115
Alpha 1 antitrypsin deficiency (AATD) is a well recognized genetic risk factor for pulmonary disease and less common liver
disease. The two most common deficiency alleles worldwide PI*S and PI*Z can be easily detected using several molecular methods.
However, there are at least 30 other AATD variants, which are only detectable by alpha 1 antitrypsin (AAT) gene sequencing
and, therefore, seem to be more under-recognized than the PI*S and PI*Z alleles. PI*Mmalton is the most frequent AATD variant
in different regions of the Southern Mediterranean basin countries, where its prevalence seems to prevail over PI*S and PI*Z.
In this work, we report the development of a simple PCR-based analysis designed for the detection of the PI*Mmalton deficiency
alleles using two specific primers. A one-tube reaction enables the distinction between the different genotypes. This reliable,
easy, fast, and low-cost technique might be useful for laboratories involved in the study of AATD-related diseases, especially
those of the Southern Mediterranean basin area with modest budget or where sophisticated equipment is not available. This
will allow larger targeted screening for PI*Mmalton in order to better understand this mutation epidemiology and its origin. 相似文献
185.
Baraket Ghada Ben Abdelkrim Ahmed Chatti Khaled Saddoud Olfa Mars Messaoud Trifi Mokhtar Salhi-Hannachi Amel 《Biochemical Systematics and Ecology》2010
Here, we report the nucleotide variation in two non-coding regions of the chloroplast DNA (cpDNA) to construct a possible evolutionary scenario in Ficus carica L. Our results suggest the occurrence of haplotypic and nucleotide diversity with a large variation level of chloroplast non-coding regions. Furthermore, our results demonstrated an explicit rejection of the null hypothesis that within F. carica the intron trnL and the spacer trnL-trnF evolved under a strictly neutral model of molecular evolution. Although, recent population expansion could serve as one alternative explanation for the detected excess of singleton, our results imply a positive selection and the genetic hitchhiking effect is unlikely. Parameters performed supported scenario of sweep selection and recent expansion of F. carica across Tunisia. Our results indicate that both positive selection and demographic histories have jointly contributed to the observed patterns of nucleotide diversity and haplotypes structure. Based on the results, we characterize the fig resources and provide several suggestions for effective conservation and improvement programs. 相似文献
186.
Taoufik Khalfaoui Nuria Basora Amel Ouertani-Meddeb 《Journal of molecular histology》2010,41(2-3):143-152
The expression of apoptotic factors Bcl-2 and Bax were studied in the conjunctiva of diabetic patients with and without retinopathy. All patients underwent a complete ophthalmic examination including ocular fundus and retinal fluorescein angiography. The indirect immunoperoxidase method was performed on 15 normal conjunctiva taken during cataract surgery (group 1), on 40 eyes of 40 patients with type 2 diabetes without diabetic retinopathy (group 2) and 13 eyes of 13 patients with diabetic retinopathy (group 3). In normal human conjunctiva, Bax showed positive expression in epithelial, vascular and stromal cells whereas Bcl-2 staining was negative. In the conjunctiva of diabetic patients without diabetic retinopathy, Bax was widely, and strongly, expressed in epithelial cells, vascular endothelial cells, fibroblasts and infiltrating cells such as macrophages. For patients with diabetic retinopathy, Bax was consistently strong to very strong. Bcl-2 protein expression became weak to negative for diabetic patients both with and without diabetic retinopathy. Immunoreactivity was not correlated between Bcl-2 and Bax in the conjunctiva of diabetic patients. Bax was always localized in tissues characterized by a high rate of apoptosis, whereas, Bcl-2 was absent. Our results suggest that diabetic human conjunctiva, with its inflammatory phenomena, is considered as a privileged target for programmed cell death. 相似文献
187.
Hadj-Bouazza A Teste K Colombeau L Chaleix V Zerrouki R Kraemer M Sainte Catherine O 《Nucleosides, nucleotides & nucleic acids》2008,27(5):439-448
The synthesis and biological activity of a novel DNA cross-linking antitumor agent is presented. The new alkylating agent significantly inhibited cell proliferation, migration and invasion as tested in vitro on the A431 vulvar epidermal carcinoma cell line. 相似文献
188.
Colombeau L Teste K Hadj-Bouazza A Chaleix V Zerrouki R Kraemer M Catherine OS 《Nucleosides, nucleotides & nucleic acids》2008,27(2):110-120
The synthesis and biological activity of chloroethyl pyrimidine nucleosides is presented. One of these new nucleosides analogues significantly inhibited cell proliferation, migration and invasion as tested in vitro on the A431 vulvar epidermal carcinoma cell line. 相似文献
189.
Ketata I Denier X Hamza-Chaffai A Minier C 《Comparative biochemistry and physiology. Toxicology & pharmacology : CBP》2008,147(3):261-270
Research on endocrine disruption has been a major topic of the past decade. Although most studies concentrated on vertebrate species, invertebrates are now gaining more attention. In particular, data on molluscs is increasing. One of the best-documented and more relevant examples of endocrine disruption is the imposex phenomenon affecting some gastropod species. But the increasing interest is also due to the fact that molluscs, especially bivalves, are good bioindicators used for decades in environmental studies and that progress have been made in the understanding of the physiology and endocrinology of some mollusc species. Recent results suggest that molluscs can be adversely affected by compounds that alter their reproduction and that vertebrate-type sex-steroids metabolism or mechanism of action could be involved in these effects. Nevertheless, the endocrine system of molluscs appears to be dissimilar in many aspects to those of vertebrates and sex-steroids might not have the same importance in all mollusc species. This diversity constitutes an important opportunity to examine and understand new and alternative mechanisms for endocrine disruption. 相似文献
190.
Ten simple rules for organizing a bioinformatics training course in low- and middle-income countries
Benjamin Moore Patricia Carvajal-Lpez Paballo Abel Chauke Marco Cristancho Victoria Dominguez Del Angel Selene L. Fernandez-Valverde Amel Ghouila Piraveen Gopalasingam Fatma Zahra Guerfali Alice Matimba Sarah L. Morgan Guilherme Oliveira Verena Ras Alejandro Reyes Javier De Las Rivas Nicola Mulder 《PLoS computational biology》2021,17(8)