全文获取类型
收费全文 | 281篇 |
免费 | 20篇 |
出版年
2022年 | 7篇 |
2021年 | 2篇 |
2020年 | 4篇 |
2017年 | 1篇 |
2016年 | 1篇 |
2015年 | 9篇 |
2014年 | 3篇 |
2013年 | 12篇 |
2012年 | 18篇 |
2011年 | 20篇 |
2010年 | 17篇 |
2009年 | 16篇 |
2008年 | 21篇 |
2007年 | 16篇 |
2006年 | 11篇 |
2005年 | 25篇 |
2004年 | 12篇 |
2003年 | 14篇 |
2002年 | 13篇 |
2001年 | 6篇 |
2000年 | 6篇 |
1999年 | 4篇 |
1998年 | 1篇 |
1997年 | 3篇 |
1996年 | 5篇 |
1995年 | 8篇 |
1994年 | 4篇 |
1993年 | 2篇 |
1991年 | 1篇 |
1990年 | 5篇 |
1989年 | 4篇 |
1988年 | 2篇 |
1987年 | 2篇 |
1986年 | 1篇 |
1985年 | 3篇 |
1984年 | 3篇 |
1983年 | 1篇 |
1982年 | 1篇 |
1981年 | 2篇 |
1980年 | 1篇 |
1979年 | 1篇 |
1978年 | 1篇 |
1976年 | 1篇 |
1972年 | 1篇 |
1971年 | 2篇 |
1962年 | 1篇 |
1910年 | 1篇 |
1899年 | 1篇 |
1897年 | 2篇 |
1895年 | 1篇 |
排序方式: 共有301条查询结果,搜索用时 15 毫秒
161.
Federico Cappa Giulia Torrini Giuseppe Mazza Alberto Francesco Inghilesi Claudia Benvenuti Leonardo Viliani Pio Federico Roversi Rita Cervo 《Insect Science》2020,27(5):1031-1042
Parasites and pathogens can follow different patterns of infection depending on the host developmental stage or sex. In fact, immune function is energetically costly for hosts and trade‐offs exist between immune defenses and life history traits as growth, development and reproduction and organisms should thus optimize immune defense through their life cycle according to their developmental stage. Identifying the most susceptible target and the most virulent pathogen is particularly important in the case of insect pests, in order to develop effective control strategies targeting the most vulnerable individuals with the most effective control agent. Here, we carried out laboratory tests to identify the most susceptible target of infection by infecting different stages of the red palm weevil Rhynchophorus ferrugineus (larvae, pupae, male, and female adults) with both a generic pathogen, antibiotic‐resistant Gram‐negative bacteria Escherichia coli XL1‐Blue, and two specific strains of entomopathogenic nematodes (EPNs), Steinernema carpocapsae ItS‐CAO1 and Heterorhabditis bacteriophora ItH‐LU1. By evaluating bacterial clearance, host mortality and parasite progeny release, we demonstrate that larvae are more resistant than adults to bacterial challenge and they release less EPNs progeny after infection despite a higher mortality compared to adults. Considering the two EPN strains, S. carpocapsae was more virulent than H. bacteriophora both in terms of host mortality and more abundant progeny released by hosts after death. The outcomes attained with unspecific and specific pathogens provide useful information for a more efficient and sustainable management of this invasive pest. 相似文献
162.
Purification and characterization of aconitase isoforms from etiolated pumpkin cotyledons 总被引:3,自引:0,他引:3
Luigid De Bellis Ryuji Tsugeki Amedeo Alpi Mikio Nishimura 《Physiologia plantarum》1993,88(3):485-492
Although aconitase (EC 4.2.1.3) is involved in the glyoxylate cycle, which is localized in glyoxysomes, we detected very low aconitase activity in glyoxysomal fractions after sucrose gradient centrifugation of extracts prepared from etiolated pumpkin ( Cucurbita sp.) colyledons. Two aconitase isoforms were purified to homogeneity, albeit in low yield, by hydrophobic interaction, hydroxylapatite and anion exchange chromatography. They were designated Aco I and Aco II; both were shown to be monomeric proteins of M1 100 000 or 98 000 by gel filtration and SDS-PAGE analysis, respectively; isoelectric points were 5.0 and 4.8, respectively. Kinetic studies revealed similarities between Aco I and Aco II. A third aconitase isoform (Aco III) was revealed but not purified to homogeneity. 相似文献
163.
Valentina Cappelletti Thomas Hauser Ilaria Piazza Monika Pepelnjak Liliana Malinovska Tobias Fuhrer Yaozong Li Christian Dörig Paul Boersema Ludovic Gillet Jan Grossbach Aurelien Dugourd Julio Saez-Rodriguez Andreas Beyer Nicola Zamboni Amedeo Caflisch Natalie de Souza Paola Picotti 《Cell》2021,184(2):545-559.e22
- Download : Download high-res image (211KB)
- Download : Download full-size image
164.
165.
A Capetti S Landonio P Meraviglia A Di Biagio S Lo Caputo G Sterrantino A Ammassari B Menzaghi M Franzetti GV De Socio G Pellicanò E Mazzotta A Soria M Meschiari M Trezzi L Sasset BM Celesia P Zucchi S Melzi E Ricci G Rizzardini 《PloS one》2012,7(7):e39222
Background
Long term efficacy of raltegravir (RAL)-including regimens in highly pre-treated HIV-1-infected patients has been demonstrated in registration trials. However, few studies have assessed durability in routine clinical settings.Methods
Antiretroviral treatment-experienced patients initiating a RAL-containing salvage regimen were enrolled. Routine clinical and laboratory follow-up was performed at baseline, week 4, 12, and every 12 weeks thereafter. Data were censored at week 96.Results
Out of 320 patients enrolled, 292 (91.25%) subjects maintained their initial regimen for 96 weeks; 28 discontinued prematurely for various reasons: death (11), viral failure (8), adverse events (5), loss to follow-up (3), consent withdrawal (1). Eight among these 28 subjects maintained RAL but changed the accompanying drugs. The mean CD4+ T-cell increase at week 96 was 227/mm3; 273 out of 300 patients (91%), who were still receiving RAL at week 96, achieved viral suppression (HIV-1 RNA <50 copies/mL). When analyzing the immuno-virologic outcome according to the number of drugs used in the regimen, 2 (n = 45), 3 (n = 111), 4 (n = 124), or >4 (n = 40), CD4+ T-cell gain was similar across strata: +270, +214, +216, and +240 cells/mm3, respectively, as was the proportion of subjects with undetectable viral load. Laboratory abnormalities (elevation of liver enzymes, total cholesterol and triglycerides) were rare, ranging from 0.9 to 3.1%. The mean 96-week total cholesterol increase was 23.6 mg/dL.Conclusions
In a routine clinical setting, a RAL-based regimen allowed most patients in salvage therapy to achieve optimal viral suppression for at least 96 weeks, with relevant immunologic gain and very few adverse events. 相似文献166.
Amedeo A. Fantini 《Genetics》1962,47(2):161-177
167.
Asproni B Murineddu G Pau A Pinna GA Langgård M Christoffersen CT Nielsen J Kehler J 《Bioorganic & medicinal chemistry》2011,19(1):642-649
A series of phenylimidazole-pyrazolo[1,5-c]quinazolines 1a-q was designed, synthesized and characterised as a novel class of potent phophodiesterase 10A (PDE10A) inhibitors. In this series, 2,9-dimethyl-5-(2-(1-methyl-4-phenyl-1H-imidazol-2-yl)ethyl)pyrazolo[1,5-c]quinazoline (1q) showed the highest affinity for PDE10A enzyme (IC50 = 16 nM). 相似文献
168.
169.
170.
Andreas Schuster Matthias Paul Nuno Bettencourt Shazia T. Hussain Geraint Morton Shelby Kutty Boris Bigalke Amedeo Chiribiri Divaka Perera Eike Nagel Philipp Beerbaum 《PloS one》2015,10(4)