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排序方式: 共有903条查询结果,搜索用时 15 毫秒
891.
STBD1 (starch-binding domain-containing protein 1) belongs to the CBM20 (family 20 carbohydrate binding module) group of proteins, and is implicated in glycogen metabolism and autophagy. However, very little is known about its regulation or interacting partners. Here, we show that the CBM20 of STBD1 is crucial for its stability and ability to interact with glycogen-associated proteins. Mutation of a conserved tryptophan residue (W293) in this domain abolished the ability of STBD1 to bind to the carbohydrate amylose. Compared with the WT (wild-type) protein, this mutant exhibited rapid degradation that was rescued upon inhibition of the proteasome. Furthermore, STBD1 undergoes ubiquitination when expressed in COS cells, and requires the N-terminus for this process. In contrast, inhibition of autophagy did not significantly affect protein stability. In overexpression experiments, we discovered that STBD1 interacts with several glycogen-associated proteins, such as GS (glycogen synthase), GDE (glycogen debranching enzyme) and Laforin. Importantly, the W293 mutant of STBD1 was unable to do so, suggesting an additional role for the CBM20 domain in protein–protein interactions. In HepG2 hepatoma cells, overexpressed STBD1 could associate with endogenous GS. This binding increased during glycogenolysis, suggesting that glycogen is not required to bridge this interaction. Taken together, our results have uncovered new insights into the regulation and binding partners of STBD1. 相似文献
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893.
894.
Amber Davis 《Inorganica chimica acta》2004,357(12):3493-3502
Titanium complexes based on the (R,R)-N,N′-bis(2-hydroxy-3-functionalised-benzylidene)-1,2-diaminocyclohexane ligand and containing either chloro or bridged oxo co-ligands have been prepared, subjected to single crystal X-ray analysis and examined as pre-catalysts for the asymmetric phospho-aldol (PA) reaction. Catalysis does take place although at a much slower rate than with related aluminium complexes and then only to afford racemic products; significant observations that lead to an important design point in PA pre-catalysts. 相似文献
895.
896.
Laura A. Elenich D. Nandi A. Elizabeth Kent T. Scott McCluskey M. Cruz Mohan N. Iyer Elaine C. Woodward Christopher W. Conn Amber L. Ochoa David B. Ginsburg J. J. Monaco 《Immunogenetics》1999,49(10):835-842
The proteasome is a large multicatalytic proteinase that plays a role in the generation of peptides for presentation by major
histocompatibility complex class I molecules. The 20S proteolytic core of mammalian proteasomes is assembled from a group
of 17 protein subunits that generate a distinctive pattern of spots upon two-dimensional gel electrophoresis. The genes for
most of these subunits have been cloned from humans and rats. We isolated cDNA clones for the mouse orthologues of ten of
the subunits [PSMA1 (C2), PSMA2 (C3), PSMA3 (C8), PSMA4 (C9), PSMA5 (ZETA), PSMA6 (IOTA), PSMA7 (C6-I), PSMB2 (C7-I), PSMB3
(C10-II), and PSMB5 (X)] to complete the cloning of all of the mouse subunits. Using antisera raised against these subunits
or their orthologues, we verified the identity of these proteins by two-dimensional NEPHGE-PAGE.
Received: 8 March 1999 / Accepted: 8 April 1999 相似文献
897.
Background
RNA-binding motif protein 19 (RBM19, NCBI Accession # NP_083038) is a conserved nucleolar protein containing 6 conserved RNA recognition motifs. Its biochemical function is to process rRNA for ribosome biogenesis, and it has been shown to play a role in digestive organ development in zebrafish. Here we analyzed the role of RBM19 during mouse embryonic development by generating mice containing a mutation in the Rbm19 locus via gene-trap insertion. 相似文献898.
Evan NairGill Massimo Bonora Xue Zhong Aijie Liu Amber Miranda Nathan Stewart Sara Ludwig Jamie Russell Thomas Gallagher Paolo Pinton Bruce Beutler 《The EMBO journal》2021,40(9)
Endoplasmic reticulum (ER) calcium (Ca2+) stores are critical to proteostasis, intracellular signaling, and cellular bioenergetics. Through forward genetic screening in mice, we identified two members of a new complex, Pacs1 and Wdr37, which are required for normal ER Ca2+ handling in lymphocytes. Deletion of Pacs1 or Wdr37 caused peripheral lymphopenia that was linked to blunted Ca2+ release from the ER after antigen receptor stimulation. Pacs1‐deficient cells showed diminished inositol triphosphate receptor expression together with increased ER and oxidative stress. Mature Pacs1 −/− B cells proliferated and died in vivo under lymphocyte replete conditions, indicating spontaneous loss of cellular quiescence. Disruption of Pacs1‐Wdr37 did not diminish adaptive immune responses, but potently suppressed lymphoproliferative disease models by forcing loss of quiescence. Thus, Pacs1‐Wdr37 plays a critical role in stabilizing lymphocyte populations through ER Ca2+ handling and presents a new target for lymphoproliferative disease therapy. 相似文献
899.
900.
We report a phenomenon wherein induction of cell death by a variety of means in wing imaginal discs of Drosophila larvae resulted in the activation of an anti-apoptotic microRNA, bantam. Cells in the vicinity of dying cells also become harder to kill by ionizing radiation (IR)-induced apoptosis. Both ban activation and increased protection from IR required receptor tyrosine kinase Tie, which we identified in a genetic screen for modifiers of ban. tie mutants were hypersensitive to radiation, and radiation sensitivity of tie mutants was rescued by increased ban gene dosage. We propose that dying cells activate ban in surviving cells through Tie to make the latter cells harder to kill, thereby preserving tissues and ensuring organism survival. The protective effect we report differs from classical radiation bystander effect in which neighbors of irradiated cells become more prone to death. The protective effect also differs from the previously described effect of dying cells that results in proliferation of nearby cells in Drosophila larval discs. If conserved in mammals, a phenomenon in which dying cells make the rest harder to kill by IR could have implications for treatments that involve the sequential use of cytotoxic agents and radiation therapy. 相似文献