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排序方式: 共有277条查询结果,搜索用时 15 毫秒
61.
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Isolation of a yeast gene, SRH1, that encodes a homologue of the 54K subunit of mammalian signal recognition particle 总被引:12,自引:0,他引:12
A 1.7 kilobase HindIII fragment of Saccharomyces cerevisiae DNA was cloned by cross-hybridization with the Escherichia coli secY gene. The complete nucleotide sequence of the 2.6 kb fragment of the yeast genomic DNA containing the cross-hybridizing HindIII fragment was determined. The sequence showed no apparent similarity with that of the E. coli secY gene with the exception of a completely matched sequence of 21 bp, but it contained a 1,623 nucleotide open reading frame coding for a protein of 541 amino acids with a calculated Mr of 59,600. The N-terminal portion of 303 residues of the predicted sequence was homologous to the cytosolic domain of the alpha-subunit of the signal recognition particle receptor (SR alpha), including consensus sequence elements for a GTP binding site, whereas the C-terminal portion of 238 residues had an unusual methionine-rich domain containing several repetitive sequences. An mRNA of 2.0 kb was detected on Northern blotting analysis. The predicted sequence was 48% identical with the reported sequences of the 54K subunit of the mammalian signal recognition particle (SRP54) (Romisch K. et al. (1989) Nature 340, 478-483; Bernstein, H.D. et al. (1989) Nature 340, 482-486). We designated this gene as SRH1 (SRP54 homologue). Gene disruption experiments showed that the SRH1 gene product is essential for cell growth. 相似文献
63.
Sara OLIVáN Ana Cristina CALVO Amaya RANDO María Jesús MU?OZ Pilar ZARAGOZA Rosario OSTA 《Experimental Animals》2015,64(2):147-153
In preclinical trials, a sensitive functional test is required to detect changes in the
motor behaviour of the SOD1G93A mouse model of amyotrophic lateral sclerosis (ALS). We
evaluated changes in body weight and motor impairment in behavioural tests, such as the
rotarod, the hanging-wire test and the treadmill, of transgenic and wild type mice. We
found differences in detection of the onset of symptoms and progression of the disease
between the different tests assessed. Moreover, the data showed significant gender
differences in the motor behaviour of this mouse model. The rotarod and the hanging-wire
test were more sensitive to detect early motor impairment. Moreover, the results suggested
that the rotarod and hanging-wire became the most accurate tests rather than treadmill to
characterise the ALS disease phenotype. 相似文献
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Engel T Gomez-Villafuertes R Tanaka K Mesuret G Sanz-Rodriguez A Garcia-Huerta P Miras-Portugal MT Henshall DC Diaz-Hernandez M 《FASEB journal》2012,26(4):1616-1628
Prolonged seizures [status epilepticus (SE)] constitute a neurological emergency that can permanently damage the brain. SE results from a failure of the normal mechanisms to terminate seizures; in particular, γ-amino butyric acid-mediated inhibition, and benzodiazepine anticonvulsants are often incompletely effective. ATP acts as a fast neurotransmitter via ionotropic ligand-gated P2X receptors. Here we report that SE induced by intra-amygdala kainic acid in mice selectively increased hippocampal levels of P2X7 receptors relative to other P2X receptors. Using transgenic P2X7 reporter mice expressing enhanced green fluorescent protein, we identify dentate granule neurons as the major cell population transcribing the P2X7 receptor after SE. Pretreatment of mice with an intracerebroventricular microinjection of 1.75 nmol A438079, a P2X7 receptor antagonist, reduced seizure duration by 58% and reduced seizure-induced neuronal death by 61%. Injection of brilliant blue G (1 pmol), another selective antagonist, reduced seizure duration by 48% and was also neuroprotective. A438079 was seizure-suppressive when injected shortly after induction of SE, and coinjection of A438079 with lorazepam 60 min after triggering SE, when electrographic seizure-responsiveness to lorazepam had decreased, also terminated SE. Our results suggest that P2X7 receptor antagonists may be a promising class of drug for seizure abrogation and neuroprotection in SE. 相似文献
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Roberto Fraile Ana Isabel Calvo Amaya Castro Delia Fernández-González Eduardo García-Ortega 《Aerobiologia》2006,22(1):35-45
Long-range transport of gases and aerosols depends on vertical and horizontal air shifts: studies on atmospheric transport
have to be based on the meteorological conditions of the environment. The aim of this paper is to review the physical principles
on which meteorological models are based, and to make an exhaustive inventory of the main models used for the prognosis and
diagnosis of air masses and a list of the long-range transport models associated to them. URL addresses are provided for all
of these models to allow the reader to check their usefulness. A practical application is included with reference to trajectories
followed by air masses that reach the Iberian Peninsula at various altitudes, depending on the synoptic meteorological situation.
Further examples describe air masses entering Europe with desert dust from Africa. This dust may eventually develop into freezing
nuclei and form hailstones in summer storms. 相似文献
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Fibroblast growth factor (FGF) signalling has been implicated during several phases of early embryogenesis, including the patterning of the embryonic axes, the induction and/or maintenance of several cell lineages and the coordination of morphogenetic movements. Here, we summarise our current understanding of the regulation and roles of FGF signalling during early vertebrate development. 相似文献
70.