全文获取类型
收费全文 | 12349篇 |
免费 | 1127篇 |
国内免费 | 4篇 |
出版年
2023年 | 46篇 |
2022年 | 148篇 |
2021年 | 323篇 |
2020年 | 199篇 |
2019年 | 217篇 |
2018年 | 270篇 |
2017年 | 239篇 |
2016年 | 394篇 |
2015年 | 662篇 |
2014年 | 678篇 |
2013年 | 824篇 |
2012年 | 1034篇 |
2011年 | 942篇 |
2010年 | 573篇 |
2009年 | 538篇 |
2008年 | 762篇 |
2007年 | 708篇 |
2006年 | 571篇 |
2005年 | 593篇 |
2004年 | 531篇 |
2003年 | 544篇 |
2002年 | 450篇 |
2001年 | 114篇 |
2000年 | 85篇 |
1999年 | 127篇 |
1998年 | 138篇 |
1997年 | 102篇 |
1996年 | 87篇 |
1995年 | 75篇 |
1994年 | 84篇 |
1993年 | 90篇 |
1992年 | 65篇 |
1991年 | 81篇 |
1990年 | 56篇 |
1989年 | 58篇 |
1988年 | 62篇 |
1987年 | 60篇 |
1986年 | 62篇 |
1985年 | 66篇 |
1984年 | 52篇 |
1983年 | 52篇 |
1982年 | 66篇 |
1981年 | 65篇 |
1980年 | 58篇 |
1979年 | 39篇 |
1978年 | 39篇 |
1977年 | 43篇 |
1976年 | 34篇 |
1973年 | 30篇 |
1969年 | 29篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
931.
D. E. Chamberlain A. Joys P. J. Johnson L. Norton R. E. Feber R. J. Fuller 《Biology letters》2010,6(1):82-84
The generally higher biodiversity on organic farms may be influenced by management features such as no synthetic pesticide and fertilizer inputs and/or by differences in uncropped habitat at the site and landscape scale. We analysed bird and habitat data collected on 48 paired organic and conventional farms over two winters to determine the extent to which broad-scale habitat differences between systems could explain overall differences in farmland bird abundance. Density was significantly higher on organic farms for six out of 16 species, and none on conventional. Total abundance of all species combined was higher on organic farms in both years. Analyses using an information-theoretic approach suggested that both habitat extent and farm type were important predictors only for starling and greenfinch. Organic farming as currently practised may not provide significant benefits to those bird species that are limited by winter food resources, in particular, several declining granivores. 相似文献
932.
Sean C Sleight Bryan A Bartley Jane A Lieviant Herbert M Sauro 《Journal of biological engineering》2010,4(1):12
Background
One problem with engineered genetic circuits in synthetic microbes is their stability over evolutionary time in the absence of selective pressure. Since design of a selective environment for maintaining function of a circuit will be unique to every circuit, general design principles are needed for engineering evolutionary robust circuits that permit the long-term study or applied use of synthetic circuits. 相似文献933.
Multilocus species identification and fungal DNA barcoding: insights from blue stain fungal symbionts of the mountain pine beetle 总被引:1,自引:0,他引:1
There is strong community-wide interest in applying molecular techniques to fungal species delimitation and identification, but selection of a standardized region or regions of the genome has not been finalized. A single marker, the ribosomal DNA internal transcribed spacer region, has frequently been suggested as the standard for fungi. We used a group of closely related blue stain fungi associated with the mountain pine beetle (Dendroctonus ponderosae Hopkins) to examine the success of such single-locus species identification, comparing the internal transcribed spacer with four other nuclear markers. We demonstrate that single loci varied in their utility for identifying the six fungal species examined, while use of multiple loci was consistently successful. In a literature survey of 21 similar studies, individual loci were also highly variable in their ability to provide consistent species identifications and were less successful than multilocus diagnostics. Accurate species identification is the essence of any molecular diagnostic system, and this consideration should be central to locus selection. Moreover, our study and the literature survey demonstrate the value of using closely related species as the proving ground for developing a molecular identification system. We advocate use of a multilocus barcode approach that is similar to the practice employed by the plant barcode community, rather than reliance on a single locus. 相似文献
934.
In the 1990s, liver transplantation for hepatitis B and C virus (HBV and HCV) related-liver diseases was a very controversial issue since recurrent infection of the graft was inevitable. Significant progress has been made in the prophylaxis and treatment of recurrent hepatitis B/C (or HBV/HCV infection) after liver transplantation. In this paper, we propose a mathematical model of ordinary differential equations describing the dynamics of the HBV/HCV and its interaction with both liver and blood cells. A single model is used to describe infection of either virus since the dynamics in-host (infected of the liver) are similar. Analyzing the model, we observe that the system shows either a transcritical or a backward bifurcation. Explicit conditions on the model parameters are given for the backward bifurcation to be present. Consequently, we investigate possible factors that are responsible for HBV/HCV infection and assess control strategies to reduce HBV/HCV reinfection and improve graft survival after liver transplantation. 相似文献
935.
Species with complex life cycles pose challenges for understanding what processes regulate population densities, especially if some life stages disperse. Most studies of such animals that are thought to be recruitment limited focus on the idea that juvenile mortality limits the density of recruits (and hence population density), fewer consider the possibility that egg supply may be important. For species that oviposit on specific substrata, environmental constraints on oviposition sites may limit egg supply. Female mayflies in the genus Baetis lay egg masses on the underside of stream rocks that emerge above the water’s surface. We tested the hypothesis that egg mass densities are constrained by emergent rock densities within and between streams, by counting egg masses on emergent rocks. All emergent rocks were counted along 1-km lengths of four streams, revealing significant variation in emergent rock density within streams and a more than three-fold difference between streams. In each stream, egg mass density increased with the density of emergent rocks in 30-m stretches. We used regression equations describing these small-scale relationships, coupled with the large-scale spatial variation of emergent rocks, to estimate egg mass densities for each 1-km stream length, a scale relevant to population processes. Scaled estimates were positively associated with emergent rock density and provided better estimates than methods that ignored environmental variation. Egg mass crowding was inversely related to emergent rock density at the stream scale, a pattern consistent with the idea that oviposition substrata were in short supply in streams with few emergent rocks, but crowding did not compensate entirely for differences in emergent rock densities. The notion that egg supply, not larval mortality, may limit population density is an unusual perspective for stream insects. Environmental constraints on egg supply may be widespread among other species with specialised oviposition behaviours. 相似文献
936.
Antony M. Hooper Muniru K. Tsanuo Keith Chamberlain Kay Tittcomb Julie Scholes Ahmed Hassanali Zeyaur R. Khan John. A. Pickett 《Phytochemistry》2010,71(8-9):904-908
In East African small-holder farming of maize, the cattle forage legume, Desmodium uncinatum is used as an intercrop due to its allelopathic inhibition of parasitism by Striga hermonthica, an obligate parasitic weed that can devastate the maize crop. Bioassay-guided fractionation of the root extract of D. uncinatum revealed isoschaftoside to be the main compound in the most potent fraction inhibiting growth of germinated S. hermonthica radicles. Bioassays repeated with isoschaftoside isolated from a different plant source, Passiflora incarnata, proved it to be a biologically active component. Analysis of the root exudates produced by hydroponically grown D. uncinatum showed isoschaftoside to be present in the hydroponic media at biologically active concentrations of 10–100 nM. 相似文献
937.
Plant neighbor identity influences plant biochemistry and physiology related to defense 总被引:1,自引:0,他引:1
Amanda K Broz Corey D Broeckling Clelia De-la-Peña Matthew R Lewis Erick Greene Ragan M Callaway Lloyd W Sumner Jorge M Vivanco 《BMC plant biology》2010,10(1):115
Background
Chemical and biological processes dictate an individual organism's ability to recognize and respond to other organisms. A small but growing body of evidence suggests that plants may be capable of recognizing and responding to neighboring plants in a species specific fashion. Here we tested whether or not individuals of the invasive exotic weed, Centaurea maculosa, would modulate their defensive strategy in response to different plant neighbors. 相似文献938.
939.
John P Zajicek Wendy M Ingram Jane Vickery Siobhan Creanor Dave E Wright Jeremy C Hobart 《BMC neurology》2010,10(1):88
Background
There is a need for greater understanding of the impact of multiple sclerosis (MS) from the perspective of individuals with the condition. The South West Impact of MS Project (SWIMS) has been designed to improve understanding of disease impact using a patient-centred approach. The purpose is to (1) develop improved measurement instruments for clinical trials, (2) evaluate longitudinal performance of a variety of patient-reported outcome measures, (3) develop prognostic predictors for use in individualising drug treatment for patients, particularly early on in the disease course. 相似文献940.
Buckner JH 《Nature reviews. Immunology》2010,10(12):849-859
A lack of regulatory T (T(Reg)) cells that express CD4, CD25 and forkhead box P3 (FOXP3) results in severe autoimmunity in both mice and humans. Since the discovery of T(Reg) cells, there has been intense investigation aimed at determining how they protect an organism from autoimmunity and whether defects in their number or function contribute to the development of autoimmunity in model systems. The next phase of investigation - that is, to define the role that defects in T(Reg) cells have in human autoimmunity - is now underway. This Review summarizes our progress so far towards understanding the role of CD4(+)CD25(+)FOXP3(+) T(Reg) cells in human autoimmune diseases and the impact that this knowledge might have on the diagnosis and treatment of these diseases. 相似文献