Amal Korrida  Juan Pablo Gutiérrez  Samuel E. Aggrey  Amal Amin‐Alami 《Zoo biology》2013,32(4):366-373

A Moroccan Houbara Bustard pedigree was analyzed to evaluate the genetic variability in captive breeding population using genealogical approaches. The whole Houbara breeding flock (WP) for the period 1993–2004 was made up of 531 birds comprising 346 females and 185 males. The reference population (RP) comprised 198 individuals ready for reproduction from 2000 to 2004 cohorts. The corresponding percentage of known ancestors was estimated as 98.23% for the parent generation, 41.19% for the grandparent generation and 7.00% for the great grandparents generation. The average generation interval for Houbara was computed as 4.64 years. Genetic variability loss per generation was ascertained using the effective population size (), the founder genome equivalent (fge), the effective number of ancestors and founders (fa) and (fe), respectively, for the RP and across each cohort. The results showed no bottleneck events in the breed but some loss of genetic variability just after the initiation of the conservation program. However, the annual effective population size based on the realized increase in inbreeding () was estimated to be 207 for the RP and 1,000 for the WP. With regard to conservation breeding schemes, the genealogical evidence presented here is very useful as it revealed the positive effect of migration on Houbara breeding. The mating strategies will assist in the future control and management of the genetic variability of this population. Zoo Biol. 32:366‐373, 2013. © 2012 Wiley Periodicals, Inc.  相似文献   

    

Olfat Shaker  Amal El‐Shehaby  Salwa Fayez  Amr Zahra  Samar Marzouk  Maissa El Raziky 《Cell biochemistry and function》2013,31(7):620-625

This study aimed to determine the relationship between osteopontin gene polymorphisms and its protein level and the efficacy of interferon‐based therapies in Hepatitis C virus (HCV) patients. Hundreds HCV patients genotype 4, treated with pegylated interferon alfa‐2b plus ribavirin and 60 healthy subjects were enrolled. All individuals were subjected to clinical and laboratory parameters, including hepatitis markers and HCV quantitation by real‐time polymerase chain reaction. Single nucleotide polymorphisms (SNPs) of osteopontin (OPN) gene (nucleotide ?155, ?443 and ?1748) were analysed by direct sequencing in addition to estimation of serum level of OPN. SNP at ?443 (C/C versus C/T, T/T) was found to represent predictors for treatment response by univariate logistic regression analysis. OPN serum level was independent predictors for treatment response by both univariate and multivariate logistic regression analysis. SNP at nucleotide ?443 and serum OPN protein levels could be used as useful markers to predict the efficacy of treatment. Copyright © 2013 John Wiley & Sons, Ltd.  相似文献   

    

Brooks MN  Rajaram MV  Azad AK  Amer AO  Valdivia-Arenas MA  Park JH  Núñez G  Schlesinger LS 《Cellular microbiology》2011,13(3):402-418

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Amal O. Amer 《Cellular microbiology》2010,12(2):140-147

Legionella pneumophila has become a model system to decipher the non‐apoptotic functions of caspases and their role in immunity. In permissive cells, the L. pneumophila‐containing vacuole evades endosomal traffic and is remodelled by the endoplasmic reticulum. Evasion of the endosomes is mediated by the Dot/Icm type IV secretion system. Upon L. pneumophila infection of genetically restrictive cells such as wild‐type (WT) C57Bl/6J murine macrophages, flagellin is sensed by the NOD‐like receptor Nlrc4 leading to caspase‐1 activation by the inflammasome complex. Then, caspase‐7 is activated downstream of the Nlrc4 inflammasome, promoting non‐apoptotic functions such as L. pneumophila‐containing phagosome maturation and bacterial degradation. Interestingly, caspase‐3 is activated in permissive cells during early stages of infection. However, caspase‐3 activation does not lead to apoptosis until late stages of infection because it is associated with potent Dot/Icm‐mediated anti‐apoptotic stimuli that render the infected cells resistant to external apoptotic inducers. Therefore, the role of caspase‐1 and non‐apoptotic functions of executioner caspases are temporally and spatially modulated during infection by L. pneumophila, which determine permissiveness to intracellular bacterial proliferation. This review will examine the novel activation pathways of caspases by L. pneumophila and discuss their role in genetic restriction and permissiveness to infection.  相似文献   

    

Shervington A  Pawar V  Menon S  Thakkar D  Patel R 《Molecular biology reports》2009,36(5):1181-1186

Telomerase expression strongly correlates with the grade of malignancy in glioma with inhibition illustrating a definite increase in chemosensitivity. This study was designed to investigate the effects of a green tea derivative, epigallocatechin-3-gallate (EGCG); together with either cisplatin or tamoxifen in glioma, and to investigate whether these effects are mediated through telomerase suppression. EGCG showed a significant cytotoxic effect on 1321N1 cells after 24 h and on U87-MG cells after 72 h (P < 0.001) without significantly affecting the normal astrocytes. Treatment with EGCG inhibited telomerase expression significantly (P < 0.01) and enhanced the effect of cisplatin and tamoxifen in both 1321N1 (P < 0.01) and U87-MG (P < 0.001) cells. EGCG, as a natural product has enormous potential to be an anti-cancer agent capable of enhancing tumour cell sensitivity to therapy.  相似文献   

    

David V. Smil  Sukhdev Manku  Yves A. Chantigny  Silvana Leit  Amal Wahhab  Theresa P. Yan  Marielle Fournel  Christiane Maroun  Zuomei Li  Anne-Marie Lemieux  Alina Nicolescu  Jubrail Rahil  Sylvain Lefebvre  Anthony Panetta  Jeffrey M. Besterman  Robert Déziel 《Bioorganic & medicinal chemistry letters》2009,19(3):688-692

In an effort to identify HDAC isoform selective inhibitors, we designed and synthesized novel, chiral 3,4-dihydroquinoxalin-2(1H)-one and piperazine-2,5-dione aryl hydroxamates showing selectivity (up to 40-fold) for human HDAC6 over other class I/IIa HDACs. The observed selectivity and potency (IC₅₀ values 10–200 nM against HDAC6) is markedly dependent on the absolute configuration of the chiral moiety, and suggests new possibilities for use of chiral compounds in selective HDAC isoform inhibition.  相似文献   

    

Oscar M. Saavedra  Ljubomir Isakovic  David B. Llewellyn  Lijie Zhan  Naomy Bernstein  Stephen Claridge  Franck Raeppel  Arkadii Vaisburg  Nadine Elowe  Andrea J. Petschner  Jubrail Rahil  Norman Beaulieu  A. Robert MacLeod  Daniel Delorme  Jeffrey M. Besterman  Amal Wahhab 《Bioorganic & medicinal chemistry letters》2009,19(10):2747-2751

The inhibitory activity of base-modified SAH analogues and the specificity of inhibiting human DNMT1 and DNMT3b2 enzymes was explored. The 6-amino group was essential while the 7-N of the adenine ring of SAH could be replaced by CH– without loss of activity against both enzymes. The introduction of small groups at the 2-position of the adenine moiety favors DNMT1 over DNMT3b2 inhibition whereas alkylation of the N⁶-amino moiety favors the inhibition of DNMT3b2 enzyme.  相似文献   

    

Taha MO  Atallah N  Al-Bakri AG  Paradis-Bleau C  Zalloum H  Younis KS  Levesque RC 《Bioorganic & medicinal chemistry》2008,16(3):1218-1235

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El Sheikh AF  Klotz MG 《Environmental microbiology》2008,10(11):3026-3035

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Zakaria M. Sawan  Mahmoud H. Mahmoud  Amal H. El-Guibali 《Journal of Plant Ecology》2008,1(4):259

Aims Supplying optimal quantities of mineral nutrients to growing crop plants is one way to improve crop yields. Nutrients need to be used rationally in order to avoid a negative ecological impact and undesirable effects on the sustainability of agricultural production systems. Excessive application of nutrients also affects the farmer's economy. In order to calculate the amount of fertilizer to be applied to crops, it is necessary to develop recommendation programmers that adjust nutrient rates to crop requirements.Methods Experiments in two successive seasons were conducted to investigate the effect of K fertilization and foliar application of Zn and P on yield and fiber properties of cotton cv. Giza 86. Potassium (0.0 and 47.4 kg of K ha^-1) was soil applied, while chelated zinc (0.0 and 57.6 g of Zn ha^-1, applied twice at 70 and 85 days after sowing 'DAS') and phosphorus (0.0, 576, 1?152 and 1?728 g of P ha^-1, applied twice at 80 and 95 DAS) were applied to the foliage.Important findings Dry matter yield, total chlorophyll concentration, K, Zn and P uptake per plant, number of opened bolls per plant, boll weight, seed index, lint index, seed cotton yield per plant, seed cotton and lint yield ha^-1 and earliness of harvest increased with the application of K, Zn and P. Treatments generally had no significant effect on lint percentage and fiber properties, with exceptions, for micronaire reading and flat bundle strength, and uniformity ratio, where the mean values of these characters were significantly increased over the untreated control by applying K, and for the micronaire reading in the first season, when applying P at 1?728 g ha^-1, and uniformity ratio in the second season, when applying P at 1?152 and 1?728 g ha^-1, where the mean values of these characters were significantly increased over the untreated control by applying P. Under the conditions of this study, applying K fertilization at 47.4 kg ha^-1 combined with spraying cotton plants with zinc at 57.6 g ha^-1 and also with P at 1?728 g ha^-1 improved growth and yield of Egyptian cotton.  相似文献